scholarly journals A Simple Spectrophotometric Method for the Determination of Thiobarbituric Acid Reactive Substances in Fried Fast Foods

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Alam Zeb ◽  
Fareed Ullah
Keyword(s):  
Acetic Acid ◽  
Spectrophotometric Method ◽  
Glacial Acetic Acid ◽  
Thiobarbituric Acid ◽  
Potato Chips ◽  
Thiobarbituric Acid Reactive Substances ◽  
Acetic Acid Medium ◽  
Fast Foods ◽  
Acetic Acid Water

A simple and highly sensitive spectrophotometric method was developed for the determination of thiobarbituric acid reactive substances (TBARS) as a marker for lipid peroxidation in fried fast foods. The method uses the reaction of malondialdehyde (MDA) and TBA in the glacial acetic acid medium. The method was precise, sensitive, and highly reproducible for quantitative determination of TBARS. The precision of extractions and analytical procedure was very high as compared to the reported methods. The method was used to determine the TBARS contents in the fried fast foods such as Shami kebab, samosa, fried bread, and potato chips. Shami kebab, samosa, and potato chips have higher amount of TBARS in glacial acetic acid-water extraction system than their corresponding pure glacial acetic acid and vice versa in fried bread samples. The method can successfully be used for the determination of TBARS in other food matrices, especially in quality control of food industries.

scholarly journals A rapid spectrophotometric method for determination of thiobarbituric acid reactive substances in rainbow trout feed

2020 ◽  
Vol 47 (1) ◽  
pp. 43-53
Author(s):  
Slađana Rakita ◽  
Dušica Čolović ◽  
Alenka Levart ◽  
Vojislav Banjac ◽  
Radmilo Čolović ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Spectrophotometric Method ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substances

scholarly journals Simultaneous Determination of Amoxycillin Trihydrate and Clavulanate Potassium in Pharmaceutical Preparations by Thin-Layer Chromatography/Densitometry

2000 ◽  
Vol 83 (6) ◽  
pp. 1493-1496 ◽  
Author(s):  
Gunawan Indrayanto ◽  
Tan Kiauw Sa ◽  
Sonja Widjaja
Keyword(s):  
Quality Control ◽  
Acetic Acid ◽  
Simultaneous Determination ◽  
Glacial Acetic Acid ◽  
Butyl Acetate ◽  
Pharmaceutical Preparations ◽  
Distilled Water ◽  
Acetic Acid Water ◽  
Layer Chromatography

Abstract A simple and rapid densitometric method has been developed for the simultaneous determination of amoxycillin trihydrate and clavulanate potassium in pharmaceutical preparations. After extraction of the analytes with distilled water, the extracts were spotted on precoated silica gel plates, which were then eluted with butyl acetate–methanol–glacial acetic acid–water (15 + 7.5 + 7.5 + 3, v/v). Quantitative evaluation was performed by measuring the absorbance reflectance of the analyte spots at λ = 240 nm. The densitometric method is rapid, selective, precise, and accurate and, thus, can be used for routine analysis of pharmaceutical preparations in quality control laboratories of the pharmaceutical industry.

scholarly journals Optimized steps in determination of malondialdehyde (MDA) standards on diagnostic of lipid peroxidation

2018 ◽  
Vol 30 (2) ◽  
pp. 136
Author(s):  
Prima Nanda Fauziah ◽  
Ani Melani Maskoen ◽  
Tri Yuliati ◽  
Erlina Widiarsih
Keyword(s):  
Lipid Peroxidation ◽  
High Sensitivity ◽  
Thiobarbituric Acid ◽  
Oxidation Products ◽  
Second Phase ◽  
Thiobarbituric Acid Reactive Substances ◽  
Acetic Acid Medium ◽  
Standard Curve ◽  
Two Phases

Introduction: Lipid peroxidation, one of the known indices of oxidative stress, is documented in various diseases. Secondary oxidation products such as malondialdehyde (MDA) is commonly measured to observe lipid peroxidation. In this study, a spectrophotometric method was evaluated to measure thiobarbituric acid reactive substances (TBARS) with high sensitivity. This study was aimed to optimisation standard of MDA using tetraethoxypropane (TEP) 97% (FW=220.3). Methods: The method is based upon the reaction of malondialdehyde (MDA) and TBA in the glacial acetic acid medium. MDA is a known biomarker of oxidative status in a biological system. This research consists of two phases: first, making a stock of TEP, and the second phase was testing the concentration of TEP for finding the standard curve of MDA before used in diagnostic of lipid peroxidation. Results: Result showed the concentration 1,875-60 uM of TEP could form a precise standard curve. Conclusion: This concentration of TEP can be used as a reference as the standard of control in diagnostic of lipid peroxidation using TBARS method.

scholarly journals Nitration of Jharia basin coals, India: a study of structural modifications by XRD and FTIR techniques

2021 ◽  
Author(s):  
Prabal Boral ◽  
Atul K. Varma ◽  
Sudip Maity
Keyword(s):  
Acetic Acid ◽  
Nitric Acid ◽  
Linear Relationship ◽  
Glacial Acetic Acid ◽  
Vitrinite Reflectance ◽  
Aqueous Media ◽  
Interlayer Spacing ◽  
Acetic Acid Medium ◽  
X Ray ◽  
The Media

AbstractFour coal samples from Jharia basin, India are treated with nitric acid in glacial acetic acid and aqueous media to find out the chemical, petrographic and spatial structure of the organic mass by X-ray diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FTIR) techniques. X-ray parameters of coal like interlayer spacing (d002), crystallite size (Lc), aroamticity (fa), average number of aromatic layers (Nc), and coal rank (I26/I20) have been determined using profile-fitting software. Considerable variation is observed in treated coals in comparison to the demineralized coals. The d002 values of treated coals have increased in both the media showing increase in disordering of organic moieties. A linear relationship has been observed between d002 values with the volatile matter of the coals. Similarly, the d002 values show linear relationship with Cdmf contents for demineralized as well as for the treated coals in both the media. The Lc and Nc values have decreased in treated coals corresponding to demineralized coals. The present study shows that nitration in both the media is capable of removing the aliphatic side chains from the coals and aromaticity (fa) increases with increase in rank and shows a linear relationship with the vitrinite reflectance. The corresponding I26/I20 values are least for treated coals in glacial acetic acid medium followed by raw and then to treated coals in aqueous medium. FTIR studies show that coal arenes of the raw coals are converted into nitro-arenes in structurally modified coals (SMCs) in both the media, the corresponding bands at 1550–1490 and 1355–1315 cm−1 respectively. FTIR study confirms that nitration is the predominant phenomenon, though, oxidation and nitration phenomena takes place simultaneously during treatment with nitric acid to form SMCs. In comparison to raw coals, the SMCs show higher aromaticity and may be easily converted to coal derived products like activated carbon and specialty carbon materials.

Micro-determination of pyrrole derivatives with N-bromosuccinimide in acetic acid medium

The Analyst ◽  
1976 ◽  
Vol 101 (1208) ◽  
pp. 867
Author(s):  
J. P. Sharma ◽  
V. K. S. Shukla ◽  
A. K. Dubey
Keyword(s):  
Acetic Acid ◽  
Acid Medium ◽  
Acetic Acid Medium ◽  
Pyrrole Derivatives

scholarly journals A spectrophotometric method for determination of fluorophore-to-protein ratios in conjugates of the blue fluorophore 7-amino-4-methylcoumarin-3-acetic acid (AMCA)

1990 ◽  
Vol 38 (1) ◽  
pp. 87-94 ◽  
Author(s):  
M W Wessendorf ◽  
S J Tallaksen-Greene ◽  
R M Wohlhueter
Keyword(s):  
Acetic Acid ◽  
Optical Density ◽  
Extinction Coefficient ◽  
Spectrophotometric Method ◽  
Extinction Coefficients ◽  
Beer's Law ◽  
Beer’S Law ◽  
The Given

7-Amino-4-methylcoumarin-3-acetic acid (AMCA) has been found to be a useful fluorophore for immunofluorescence. The present study describes a spectrophotometric method for determining the ratio of moles AMCA to moles protein (or the f/p ratio) in an AMCA-conjugated IgG. The concentration of a substance absorbing light can be determined spectrophotometrically using Beer's Law: Absorbance = Concentration x Extinction coefficient. From Beer's law, one can derive the following formula for determining the f/p ratio of AMCA-IgG conjugates: f/p = (epsilon 280IgG).A350 - (epsilon 350IgG).A280/(epsilon 350AMCA).A280 - (epsilon 280AMCA).A350 where A is the optical density of the conjugate at the given wavelength and epsilon is the extinction coefficient of a substance at the wavelength specified. Using conjugates of model proteins, it was found that the extinction coefficients of the AMCA moiety of AMCA-conjugated protein were 1.90 x 10(4) at 350 nm and 8.29 x 10(3) at 280 nm. Similarly, it was found that the extinction coefficients of swine IgG were 1.56 x 10(3) at 350 nm and 1.26 x 10(5) at 280 nm. Thus, for AMCA-conjugated swine IgG: f/p = (1.26 x 10(5)).A350 - (1.56 x 10(3)).A280/(1.47 x 10(4)).A280 - (6.42 x 10(3)).A350 [corrected]. Based on this formula, the f/p ratios of some AMCA-IgG conjugates useful for immunohistochemistry have been found to range between 6 and 24.

Reverse Phase Liquid Chromatographic Determination of Chlorophacinone and Diphacinone in Bait Formulations

1982 ◽  
Vol 65 (4) ◽  
pp. 927-929
Author(s):  
Brian R Bennett ◽  
Gregory S Grimes
Keyword(s):  
Acetic Acid ◽  
Glacial Acetic Acid ◽  
Chromatographic Determination ◽  
Reverse Phase ◽  
Target Concentration ◽  
External Standard ◽  
Liquid Chromatographic Determination ◽  
Phase Liquid ◽  
Liquid Chromatographic

Abstract Chlorophacinone and diphacinone are extracted at the 0.005% level from grain or paraffinized baits with glacial acetic acid. The target concentration is 0.01 mg/mL. The filtered supernate is chromatographed on a Partisil PXS ODS10/25 liquid chromatography column with premixed and degassed glacial acetic acid-tetrahydrofuran-water (14 + 2 + 9) and detected at 288 nm. The concentration is calculated by using an external standard. The recovery from spiked samples averaged 96.6% for both analytes. The response is linear from 0.001 to 0.040 mg/mL. The coefficient of variation of within-day replicates ranged from 1.1 to 2.5%.

Titrimetric Determination of Iodine-Bromine Numbers of Some Edible Oils Using Three N-Chloroimides

1987 ◽  
Vol 70 (4) ◽  
pp. 762-763
Author(s):  
Narayanan Jayasree ◽  
Parameswaran Indrasenan
Keyword(s):  
Acetic Acid ◽  
Reaction Time ◽  
Solid State ◽  
Palm Olein ◽  
Edible Oils ◽  
Aqueous Acetic Acid ◽  
Acetic Acid Medium ◽  
Back Titration ◽  
Titrimetric Determination

Abstract Three simple titrimetric methods have been developed to determine iodine-bromine numbers of some edible oils, such as coconut, gingelly, groundnut, mustard, olive, palm olein, and sunflower, using 3 N-chloroimides. The 3 N-chloroimides are N-chlorophthalimide, N-chlorosuccinimide, and N-chlorosaccharin, all of which have recently been developed as potential oxidimetric titrants for use in aqueous acetic acid medium. The proposed excess back-titration methods have advantages over existing methods in terms of ease of extraction into aqueous acetic acid layer, shorter reaction time of NCSA method, and stability of N-chloroimides in solid state.

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