Periodontal clinical features of bronchial asthma patient using inhalation drugs

Introduction: Bronchial asthma patients use inhalation drugs as therapy to achieve a controlled asthma state. Inhaled corticosteroids and β2 agonists are the medication that is being used in this case. Prolonged use of inhalation drugs will affect the health of periodontal tissue. The effect of these drugs can reduce the periodontal defense mechanism and increase the risk of periodontal disease. This study aims to determine the periodontal clinical features of bronchial asthma patients using inhalation drugs. Method: This research was a descriptive study. The sample of the study were 22 females and 8 males bronchial asthma patients at the Community Lung Health Center of Bandung (Balai Besar Kesehatan Paru Masyarakat Bandung/BBKPM Bandung) who had been using inhalation drugs for at least one year. The periodontal clinical feature was assessed by employing a Community Periodontal Index (CPI) modified with probing the gingival sulcus to assess the presence of gingival bleeding, periodontal pocket, and loss of clinical attachment where then a periodontal status was obtained. The periodontal status data were then processed using the periodontitis case definitions from the Centers for Disease Control and Prevention/American Academy of Periodontology (CDC/AAP) to determine periodontal disease in asthmatics patients. Results: Clinical features of gingival bleeding were found in 26 asthmatic patients (86.7%), periodontal pockets in 18 asthmatic patients (60%), and loss of clinical attachment in 27 asthmatic patients (90%). Based on the CDC/AAP case definition, 23 asthmatic patients (76.7%) had periodontitis. Bronchial asthma patients who used inhalation drugs had a risk of periodontitis.Conclusion: Bronchial asthma patients who used inhalation drugs for at least one year had gingival bleeding and loss of clinical attachment and periodontal pocket.

The potential of cassava leaves extract in gingival fibroblasts of periodontitis of rats with ovarian dysfunction

Introduction: The prevalence of periodontitis has reached 42.8% in Indonesia. One contributing factor is Porphyromonas gingivalis. The ovarian dysfunction caused by ovariectomy procedures may also cause periodontitis. Post ovariectomy conditions resemble menopause, lowering the production of estrogen and progesterone hormones, leaving patients vulnerable to periodontitis. To prevent the side effects of the treatment, herbal ingredients are commonly used. The extract of cassava leaves is known to have pharmacological effects as an antioxidant and, thus, can be applied as a medicine for periodontitis. Methods: A total of 21 rats were divided into three groups, (1) control: healthy rats; (2) Group P.1: P. gingivalis-induced rats; (3) Group P.2: ovariectomized rats. Groups P.1 and P.2 were further divided into three subgroups that were administered cassava leaves extract at a dosage of 179.2 mg/ kg BW, aquades and vitamin C. Rats were euthanized at day eight after the initial treatment to collect left mandibular first molar. The mandibular sections were immunohistochemically stained to quantify SOD expression using light microscope while the Image J software was used. Fibroblast cells expressing SOD are characterized by brown coloration in the cytoplasm and darker nucleus. Results: In periodontitis conditions, either due to P.gingivalis induction or ovarian dysfunction, fibroblast cells in rats given cassava leaves extract expressed higher SOD than rats given aquades, but still lower than rats given vitamin C. The result of the one way ANOVA test showed p value of 0,00, which means that there is a significant difference in all groups. Conclusion: Cassava leaves extract demonstrated the potential to increase the antioxidant expression in P. gingivalis-induced and ovarian dysfunction rats.

Flexural properties of electrospun polymethyl methacrylate microfiber-reinforced BisGMA for dental post prefabrication

Introduction: A dental post is a restoration to preserve the remaining tooth structure thus can be functioned normally. Many researchers suggested a fiber dental post due to its biomechanical properties that are similar to dentin structure. This study aims to analyse the flexural properties of electrospun polymethyl methacrylate microfiber-reinforced BisGMA for dental post prefabrication. Methods: The sample used was following the ADA guideline, as well as for the number of samples. The sample size was 25×2×2mm, which is close to the average dental post size. PMMA microfibers were prepared by dissolving heat cure PMMA powder with 99% acetone, then electrospinning with a rotary collector. Acquired PMMA microfibers were immersed into the resin matrix containing BisGMA, camphorquinone, and 2-dimethylaminoethyl methacrylate (DMAEMA) as a monomer, initiator, and co-initiator, respectively, to prepare the dental posts. Results: PMMA microfibers structure and surface fracture of dental posts were confirmed by Scanning Electron Microscopy (SEM). PMMA microfibers show unaligned fiber morphology with an approximate diameter size of 1-5 µm. A universal testing machine was used to measure the dental post's flexural properties (flexural strength and flexural modulus). Dental posts with PMMA fibers showed higher flexural strength (83.5 ± 10.7 MPa) compared to the dental post without PMMA fibers (61.7 ± 3.03 MPa) with a p-value <0.05. On the other hand, PMMA fibers' addition did not significantly increase the dental post's flexural modulus. Conclusion: The PMMA microfibers can intimately adhere to the BisGMA mixture as the resin matrix. Therefore, the PMMA microfiber significantly improves the flexural strength of the BisGMA for dental post prefabrication.

Antibacterial potential of strawberries and basil extracts combination against Streptococcus sanguinis (ATCC 10556)

Introduction: Streptococcus sanguinis is a commensal microorganism as well as a pioneer colony in forming dental plaque. Oral biofilm formation can be prevented by a mechanical cleaning procedure followed by the use of mouthwash. The current gold standard for mouthwash is chlorhexidine. Nevertheless, it has side effects that are not recommended for long-term use. Previous studies had proven that herbal-based mouthwashes such as basil leaves (Ocimum basilicum) and strawberry fruit (Fragaria x ananassa) have been shown to have antibacterial properties. The effectivity of antibacterial activity phenomenon in combined extracts has been reported in other studies. This research aims to observe the antibacterial potential of the F. x ananassa and O. basilicum extract combinations against S. sanguinis (ATCC 10556). Methods: The sample of this study was a combination of F. x ananassa and O. basilicum extract, which initially screened for their antibacterial activities. Antibacterial activities of F. x ananassa and O. basilicum extracts against S. sanguinis were observed using Kirby Bauer method, while Minimum Inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) by serial microdilution method. The 2% concentration from each extract was combined in 1:1, 1:2, and 2:1 volume ratio variations then tested for inhibitory zones, MIC, and MBC. Results: F. x ananassa extract had 0.125% and 0.25% for MIC and MBC respectively, while O. basilicum extract showed the value of MIC and MBC as 0.031% and 0.063% against S. sanguinis (ATCC 10556). The extract combinations in 1:1, 1:2, and 2:1 volume ratio variations showed 0.016% for MIC and 0.031% for MBC. Conclusions: It was concluded that combining extracts of 2 % F. x ananassa and 2% O. basilicum in various ratios were observably to have the antibacterial potential against S. sanguinis (ATCC 10556).

Ethanol extract of soursop leaves inhibits acid production and adhesion of Streptococcus mutans

ABSTRACTIntroduction: Dental caries and dental plaque are the most popular global oral health problems. The primary step of dental caries is characterized by damage of tooth surfaces affected by acids which are by-products of sugar metabolism by a cariogenic bacteria. One of the cariogenic bacteria is Streptococcus mutans. Annona muricata is traditionally applied as an herbal remedy for various illnesses and has been recognized in a previous study as an antimicrobial agent. The aim of this study was to investigate acid production and adhesion of ethanol extract of Soursop on Streptococcus mutans. Methods: Soursop leaf extracted by maceration using 70% ethanol solvent. The extracts obtained were tested at various concentrations. To examine the effect of ethanol extract of Soursop on acid production by S. mutans, the pH of the culture was determined using a pH meter. Inhibition of adhesion of S. mutans to the saliva-coated hydroxyapatite (S-HA) discs was quantified using colony counting on TYS20B agar plates. Results: The pH of S. mutans cultures in the presence of ethanol extract of Soursop leaf at various concentrations was higher than negative control, but there were no differences in pH value between the various concentrations of ethanol extract of Soursop leaf. Adhesion of S. mutans to S-HA discs was inhibited by various concentrations of ethanol extract of Soursop leaf. Adhesion decreased with increasing concentrations of ethanol extract of Soursop leaf, but there was not significant difference in colony count between the various concentrations of ethanol extract of Soursop leaf. Conclusions: Ethanol extract of Soursop leaf attenuates the acid production and adhesion of S. mutans to hydroxyapatite discs. Keywords: Soursop (Annona muricata); Streptococcus mutans; acid production; adhesion

Differences of anteroposterior facial dimensions in male and female children on intermediate mixed and early permanent dentition using Cervical Vertebrae Maturation Index

Introduction: Cervical vertebrae are one of the indicators for craniofacial bones maturation. Timing of craniofacial bone maturation determined achievement of orthodontic early treatment. Some previous researchers recommended cervical vertebral maturation to assess craniofacial growth. This study was aimed to analyse the differences of anteroposterior facial dimensions in male and female children on intermediate mixed and early permanent dentition using Cervical Vertebrae Maturation Index (CVMI). Methods: An analytic observational study with a cross-sectional design was conducted on the students of Islamic Elementary School (Madrasah Ibtidaiyah) in Depok district, Sleman, Yogyakarta, from July 2019 to January 2020. Subjects consisted of 22 males and 22 females aged 8-11 years, obtained by a consecutive sampling technique. The anteroposterior facial analysis was performed on the lateral cephalometry for measuring the distance of Sella turcica to Nasion (S-N) representing the anterior cranial base, Posterior Nasal Spine to Anterior Nasal Spine (PNS-ANS) representing the maxilla and Gonion-Menton (Go-Me) and Condylion-Gnathion (Co-Gn) represents the mandible. Assessment of CVMI was decided by the Hassel and Farman methods. Data were analysed by One Way ANOVA. Results: The mean value of S-N, PNS-ANS, Go-Me, and Co-Gn dimensions, generally were higher in males than females; however, only dimensions of maxillary and mandibular were showed significant difference (p<0.05), while the S-N dimension was not significantly different (p>0.05). At the interval of CVMI 3 and 4, the Go-Me and Co-Gn dimensions showed a significant difference (p<0.05) both in males and females. Conclusion: There was a difference in anteroposterior dimensions of the maxillary and mandibular in cervical vertebral maturation in children with intermediate mixed and early permanent dentition, however, no difference was found in the anterior cranial base.

Q-EEG map of parietal and frontal lobes out of brain waves recording during dental hypnosis practice

Introduction: A patient with fear and anxiety is a common case to deal with for a dentist, therefore, dental hypnosis has been widely used to ease this situation. In a hypnotized state, the human brain may easily accept any suggestion. This is projected in the brain waves. Electroencephalograph (EEG) is a brain wave recording device, reflecting several states of consciousness. Beta for conscious, alpha and theta for subconscious, and delta for sleep. Dental hypnosis puts down beta waves to alpha or theta. Quantitative Electroencephalography (Q-EEG) or brain mapping is a comprehensive analysis of (Electroencephalography, EEG) in a colored topographic map, reflecting the brain's electrical activity. The objective of this article was reporting the parietal and frontal lobes activity during dental hypnosis based on the Q-EEG mapping. Methods: The research applied a quantitative research method using observatory study. The sample was taken with an accidental sampling method, with inclusion criteria, patients with dental anxiety and exclusion criteria was patients with special need and high level of dental anxiety. Data of the EEG records was taken in January-March 2018, and processed after in Pramita laboratorium Bandung. Results: Parietal lobe affected more during the inducement than temporal lobe. During dental hypnosis, the hypnotic markers (theta and alpha states) observed from the EEG were found to be more reactive. Conclusion: Dental hypnosis effects can be observed easily using Quantitative Electroencephalography method. Dental hypnosis affects brainwaves and brain mapping which indicate relaxations of brain waves especially on parietal lobes.

Preliminary study on the potential of topical anaesthesia from betel leaf and clove leaf extract

Introduction: Betel leaf (Piper betle Linn.) and clove leaf (Syzygium aromaticum) are Indonesian herbs which are known to cause a numb sensation. The main ingredients of betel leaf, which act as an anesthetic, are caryophyllene, eugenol, methyl eugenol, eucalyptol (1.8-cineol), linalool, α-Pinene, estragole, while cloves have local anesthetic effects through their ingredients, namely eugenol, and β-Caryophyllene. Research on the benefits of these two ingredients as topical anesthetics in Indonesia is still rare. This study was aimed to describe the potential of betel leaf and clove leaf extract as a topical anesthetic in Wistar rats. Methods: This research was a laboratory experimental research with simple randomized controlled design. The sample consisted of 6 male Wistar rats, each of which was given four treatments, namely extract with a concentration of 50%, 75%, and 100%, and topical anaesthetic benzocaine 20% as a control. Each treatment was tested using an electric stimulator and observed onset, duration of action, and depth of topical anaesthesia. The data were processed using descriptive statistics with tables and graphs. Results: Betel leaf extract with a concentration of 100% had a better mean onset, duration of work, and depth of anesthesia than the 20% benzocaine control, whereas 75% clove leaf extract had the longest duration of action compared to 20% benzocaine and 100% concentration was the topical anesthetic agent with the highest anesthetic depth. Conclusion: Based on the onset, duration of action, and depth of anesthesia, betel leaf, and clove leaf have potential as topical anesthetic agents.

Functional tooth units and masticatory ability in elderly population

Introduction: The elders are the ones that most often experience tooth loss which affects masticatory function. Masticatory function can be evaluated subjectively through masticatory ability assessment. The masticatory function is influenced by the number of posterior occluding pairs; this also may be called as functional tooth units (FTUs). The purpose of this study was to describe FTUs and masticatory ability in the elderly. Methods: This research was a descriptive study with a cross-sectional study design. Subjects were taken by purposive sampling technique. The study was conducted in the Lebakgede Sub-district, Bandung City. Data mentioned were obtained by conducting interviews regarding data on subject characteristics and questionnaires of masticatory ability, as well as examining the oral cavity to obtain FTUs data. Results: Subjects obtained were 74 people consisting of 63 women (85.14%) and 11 men (14.86%). Based on the results, the average age of the research subjects was 68.49 years, and those with good masticatory ability status were 26 elderly (35.14%) with an average FTU value of 5.9 while 48 elderly (64.86%) had poor masticatory ability status with an average FTU score of 1.5, even 31 subjects (41.89%) had a zero FTU score. Conclusion: The majority of the elderly in this research mostly had a low total FTUs score which means that the masticatory ability status in the elderly tended to be poor.

Effectiveness of the novel teledentistry “HI BOGI” an android-based oral health application in increasing oral health knowledge of elementary school children

Introduction: Knowledge is the domain of behaviour. A person's good oral health behaviour must meet the elements of good oral health knowledge. Lack of oral health knowledge is one of the causes of oral dental disease. The most common oral dental disease, particularly in elementary school, is dental caries. One of the efforts to improve oral health knowledge is to be obtained from health promotion. The COVID-19 pandemic has led to many online health promotion media. One of the health promotion media is an Android-based oral health application. The purpose of this study was to determine the effectiveness of the application of oral health on knowledge of oral health in elementary school children during the COVID-19 pandemic. Methods: Using an observational analytic method with a cross-sectional design. The sampling technique used is simple random sampling. The research instrument is a questionnaire via google form and the HI BOGI application. Analysis of the data used is the Wilcoxon test. Results: The study was conducted on 143 elementary school students in Cimahi City. 55.2% are female, and 36.4% are 12 years old. The level of knowledge of oral health before downloading the HI BOGI application was in the moderate category and became good after downloading the application. There was a significant value of knowledge before and after downloading the HI BOGI application with a significant value p<0,0000. Conclusion: There is an effect of HI BOGI to increase oral health knowledge in elementary school children.