Bibliometric Analysis of Worldwide Publications on Antimalarial Drug Resistance (2006–2015)

Background. In response to international efforts to control and eradicate malaria, we designed this study to give a bibliometric overview of research productivity in antimalarial drug resistance (AMDR). Methods. Keywords related to AMDR were used to retrieve relevant literature using Scopus database. Results. A total of 976 publications with an h-index of 63 were retrieved. The number of publications showed a noticeable increase starting in the early 1990s. The USA was the most productive country with 337 publications equivalent to one-third of worldwide publications in this field. More than two-thirds of publications by the USA (236, 70.03%) were made by international collaboration. Of the top ten productive countries, two countries were from Mekong subregion, particularly Thailand and Cambodia. The Malaria Journal was the most productive journal (136, 13.93%) in this field. Mahidol University (80, 8.20%) in Thailand was the most productive institution. Seven articles in the top-ten list were about artemisinin resistance in Plasmodium falciparum, one was about chloroquine resistance, one was about sulfadoxine-pyrimethamine resistance, and the remaining one was about general multidrug resistance. Conclusion. Eradication and control of AMDR require continuing research activity to help international health organizations identify spots that require an immediate action to implement appropriate measures.

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Are Transporter Genes Other than the Chloroquine Resistance Locus (pfcrt) and Multidrug Resistance Gene (pfmdr) Associated with Antimalarial Drug Resistance?

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.49.9.3990.2005 ◽

2005 ◽

Vol 49 (9) ◽

pp. 3990-3990 ◽

Cited By ~ 1

Author(s):

Timothy J. C. Anderson ◽

Shalini Nair ◽

Huang Qin ◽

Sittaporn Singlam ◽

Alan Brockman ◽

...

Keyword(s):

Drug Resistance ◽

Multidrug Resistance ◽

Resistance Gene ◽

Antimalarial Drug ◽

Chloroquine Resistance ◽

Resistance Locus ◽

Multidrug Resistance Gene ◽

Antimalarial Drug Resistance

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Ten-Year Molecular Surveillance of Drug-Resistant Plasmodium spp. Isolated From the China–Myanmar Border

Frontiers in Cellular and Infection Microbiology ◽

10.3389/fcimb.2021.733788 ◽

2021 ◽

Vol 11 ◽

Author(s):

Tongke Tang ◽

Yanchun Xu ◽

Long Cao ◽

Penghai Tian ◽

Jiang Shao ◽

...

Keyword(s):

Drug Resistance ◽

Low Frequency ◽

Artemisinin Resistance ◽

Border Region ◽

Chloroquine Resistance ◽

Antimalarial Drug Resistance ◽

Molecular Surveillance ◽

First Choice ◽

Greater Mekong Subregion ◽

C580y Mutation

Antimalarial drug resistance has emerged as a major threat to global malaria control efforts, particularly in the Greater Mekong Subregion (GMS). In this study, we analyzed the polymorphism and prevalence of molecular markers associated with resistance to first-line antimalarial drugs, such as artemisinin, chloroquine, and pyrimethamine, using blood samples collected from malaria patients in the China–Myanmar border region of the GMS from 2008 to 2017, including 225 cases of Plasmodium falciparum and 194 cases of Plasmodium vivax. In artemisinin resistance, only the C580Y mutation with low frequency was detected in pfk13, and no highly frequent stable mutation was found in pvk12. In chloroquine resistance, the frequency of K76T mutation in pfcrt was always high, and the frequency of double mutations in pvmdr1 of P. vivax has been steadily increasing every year. In pyrimidine resistance, pfdhfr and pvdhfr had relatively more complex mutant types associated with drug resistance sites, and the overall mutation rate was still high. Therefore, artemisinin-based combination therapies are still suitable for use as the first choice of antimalarial strategy in the China–Myanmar border region in the future.

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Detection of antimalarial drug resistance polymorphisms in Plasmodium falciparum chloroquine resistance transporter and Plasmodium falciparum multidrug resistance 1 genes of Plasmodium falciparum found in Kano State, Nigeria

Calabar Journal of Health Sciences ◽

10.25259/cjhs_14_2020 ◽

2021 ◽

Vol 5 ◽

pp. 8-14

Author(s):

Al-Mukhtar Yahuza Adamu ◽

Olayeni Stephen Olonitola ◽

Helen Ileigo Inabo ◽

Ahmad Babangida Suleiman

Keyword(s):

Drug Resistance ◽

Plasmodium Falciparum ◽

Multidrug Resistance ◽

Antimalarial Drug ◽

Artemisinin Combination Therapy ◽

Chloroquine Resistance ◽

Antimalarial Drug Resistance ◽

Antimalarial Agents ◽

Chloroquine Resistance Transporter ◽

Two Samples

Objectives: In 2018, malaria claimed an estimated 380,000 lives in African region, with Nigeria accounting for 24.0% (91,368) of malaria deaths from the region. Mutations in Plasmodium falciparum chloroquine resistance transporter (Pfcrt) and P. falciparum multidrug resistance 1 (Pfmdr-1) genes had reduced the effective use of artemisinin combination therapy through the development of resistance to these antimalarial agents. Our study set out to determine the antimalarial drug resistance polymorphisms in Pfcrt and Pfmdr-1 genes of P. falciparum isolates among patients in Kano State, Nigeria. Material and Methods: Malaria positive samples were collected across the three senatorial districts of Kano State. The samples were amplified using nested polymerase chain reaction to detect the Pfcrt and Pfmdr-1 genes. The amplicons were sequenced and bioinformatic analysis was done using CLC Sequence viewer 8.0 and BioEdit sequence alignment editor to detect the single-nucleotide polymorphisms. Results: In the Pfcrt gene, CVIET haplotype was seen in 26.2% of the samples while only two samples showed the 86Y mutation in the Pfmdr-1 gene. All the 86Y mutations and majority of the CVIET haplotypes were detected in the patients from rural settings where some of them noted that they consumed modern and traditional (herbs) antimalarial agents. One sample was observed to have the CVIET haplotype and N86Y mutation while the other five CVIET haplotypes were seen in five separate samples. A new mutation V62A was found in the Pfmdr-1 gene as observed in one of the sample. Conclusion: It is imperative to ensure the rational use of the right antimalarial agents and employ continuous resistance surveillance/mapping to ensure synergy in malaria containment and elimination strategies.

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Faculty Opinions recommendation of Antimalarial drug resistance in Africa: the calm before the storm?

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.736337650.793573187 ◽

2020 ◽

Author(s):

Toshihiro Horii ◽

Nirianne Palacpac

Keyword(s):

Drug Resistance ◽

Antimalarial Drug ◽

Antimalarial Drug Resistance

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Antimalarial drug resistance: the pace quickens

Journal of Antimicrobial Chemotherapy ◽

10.1093/jac/30.5.571 ◽

1992 ◽

Vol 30 (5) ◽

pp. 571-585 ◽

Cited By ~ 157

Author(s):

N. J. White

Keyword(s):

Drug Resistance ◽

Antimalarial Drug ◽

Antimalarial Drug Resistance

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Changing Molecular Markers of Antimalarial Drug Sensitivity across Uganda

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.01818-18 ◽

2018 ◽

Vol 63 (3) ◽

Cited By ~ 5

Author(s):

Victor Asua ◽

Joanna Vinden ◽

Melissa D. Conrad ◽

Jennifer Legac ◽

Simon P. Kigozi ◽

...

Keyword(s):

Antimalarial Drug ◽

Drug Sensitivity ◽

Artemisinin Resistance ◽

Wild Type ◽

National Treatment ◽

Antimalarial Drug Resistance ◽

Content Type ◽

Increased Sensitivity ◽

Low Prevalence ◽

Poor Efficacy

ABSTRACT The potential spread of antimalarial drug resistance to Africa, in particular for artemisinins and key partner drugs, is a major concern. We surveyed Plasmodium falciparum genetic markers associated with drug sensitivity on 3 occasions at ∼6-month intervals in 2016 and 2017 at 10 sites representing a range of epidemiological settings in Uganda. For putative drug transporters, we found continued evolution toward wild-type sequences associated with increased sensitivity to chloroquine. For pfcrt K76T, by 2017 the prevalence of the wild type was >60% at all sites and >90% at 6 sites. For the pfmdr1 N86Y and D1246Y alleles, wild type prevalence ranged from 80 to 100%. We found low prevalence of K13 propeller domain mutations, which are associated with artemisinin resistance in Asia, but one mutation previously identified in northern Uganda, 675V, was seen in 2.0% of samples, including 5.5% of those from the 3 northernmost sites. Amplification of the pfmdr1 and plasmepsin2 genes, associated elsewhere with decreased sensitivity to lumefantrine and piperaquine, respectively, was seen in <1% of samples. For the antifolate targets pfdhfr and pfdhps, 5 mutations previously associated with resistance were very common, and the pfdhfr 164L and pfdhps 581G mutations associated with higher-level resistance were seen at multiple sites, although prevalence did not clearly increase over time. Overall, changes were consistent with the selective pressure of the national treatment regimen, artemether-lumefantrine, with increased sensitivity to chloroquine, and with poor efficacy of antifolates. Strong evidence for resistance to artemisinins was not seen. Continued surveillance of markers that predict antimalarial drug sensitivity is warranted.

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