scholarly journals Analytical Method Validation of Gamijakyakgamchobuja-Tang (KCHO-1, Mecasin) Preparation

2019 ◽  
Vol 2019 ◽  
pp. 1-12
Author(s):  
Tingting Wang ◽  
Seongjin Lee ◽  
Muhack Yang ◽  
Eunhye Cha ◽  
Jongwon Jang ◽  
...  
Keyword(s):  
Quality Control ◽  
Amyotrophic Lateral Sclerosis ◽  
Herbal Medicine ◽  
Analytical Method ◽  
Toxicity Test ◽  
Room Temperature ◽  
Distilled Water ◽  
Analysis Method ◽  
Analytical Method Validation ◽  
Lateral Sclerosis

Previous studies have confirmed that KCHO-1 (Mecasin) was developed to alleviate the symptoms of Amyotrophic Lateral Sclerosis (ALS). And its toxicity test has also been carried out. The aim of this study is confirming the validation and stability of concentration analysis method of the Mecasin preparations using HPLC. As a conclusion, we found that the preparations at the concentrations of 50mg/ml and 200mg/ml in sterilized distilled water were homogeneous and it was stable for 4 hours at room temperature and 7 days refrigerated condition (2~8°C). And this method for analyzing the concentration of the Mecasin preparations has been found to be suitable. This study is helpful to promote development of reliable manufacturing medicine and good researches through definitive quality control of Mecasin as complex herbal medicine, aiming to provide help for the treatment of ALS.

scholarly journals The amyotrophic lateral sclerosis 8 protein, VAP, is required for ER protein quality control

2013 ◽  
Vol 23 (8) ◽  
pp. 1975-1989 ◽  
Author(s):  
Amina Moustaqim-Barrette ◽  
Yong Q. Lin ◽  
Sreeparna Pradhan ◽  
Gregory G. Neely ◽  
Hugo J. Bellen ◽  
...  
Keyword(s):  
Quality Control ◽  
Amyotrophic Lateral Sclerosis ◽  
Protein Quality ◽  
Protein Quality Control ◽  
Lateral Sclerosis

scholarly journals Analytical Method Validation of Azadirachtin Extracted From Azadirachta Indica

2017 ◽  
Vol 2 (04) ◽  
pp. 99-106
Author(s):  
Syed Imran Ul Haq ◽  
Tribhuvan Singh ◽  
Shaik Mohammed Salman ◽  
Afifa Namreen ◽  
Anas Rasheed
Keyword(s):  
Regression Analysis ◽  
Statistical Analysis ◽  
Azadirachta Indica ◽  
Spectrophotometric Method ◽  
Drug Concentration ◽  
Analytical Method ◽  
Distilled Water ◽  
Analytical Method Validation ◽  
Accuracy And Precision

A simple UV spectrophotometric method for the determination of Azadirachtin is developed exhibiting maximum absorbance using distilled water at 542nm.The present study is based on the detection of sample drug concentration using dichloro methane as diluent. All the conditions required were optimized. Statistical analysis was carried out and results of which were satisfactory. The optical characteristics such as absorption maxima, regression analysis and correlation coefficient are obtained. Recovery studies were close to 100% that indicate the accuracy and precision of proposed method and the non-interference of the formulation excipients. All the valid parameters are summarized.

scholarly journals Traditional Korean Medicine Treatment for Patients with Wilting Disorder: A Literature Review of In Vivo Studies

2018 ◽  
Vol 2018 ◽  
pp. 1-7
Author(s):  
Sung-Jin Kim ◽  
Yeon-Cheol Park ◽  
Yong-Hyeon Baek ◽  
Byung-Kwan Seo
Keyword(s):  
Amyotrophic Lateral Sclerosis ◽  
Herbal Medicine ◽  
Myasthenia Gravis ◽  
Bee Venom ◽  
Guillain Barre Syndrome ◽  
In Vivo Studies ◽  
Traditional Korean Medicine ◽  
Korean Medicine ◽  
Lateral Sclerosis

Wilting disorder is an abnormal condition characterized by weakness and paralysis of the upper and lower extremities. Pathogenesis and treatment target of the disorder are unclear; hence, allopathic treatment is generally used to relieve the symptoms. To investigate the treatment mechanism and effect of Traditional Korean Medicine (TKM) in patients with wilting disorder, we reviewed in vivo studies that focused on the effect of TKM on the main symptoms of wilting disorder and treatment of the diseases that can cause these symptoms. We electronically searched the PubMed, Cochrane, and CNKI (China National Knowledge Infrastructure) databases using the following search terms: (weakness OR motor function disorder) (myasthenia gravis OR Guillain-Barre syndrome OR amyotrophic lateral sclerosis OR paralysis OR polymyositis OR muscular dystrophy) AND (herbal medicine OR acupuncture OR bee-venom OR pharmacoacupuncture OR electro-acupuncture OR moxibustion). We selected 11 studies that demonstrated the effect of TKM treatment on the main symptoms of wilting disorder. In these studies, inducted models of amyotrophic lateral sclerosis, myasthenia gravis, Duchenne muscular atrophy, polymyositis, and Guillain-Barre syndrome were used. With regard to treatment, herbal medicine was used in five studies, and acupuncture and bee-venom pharmacoacupuncture were used in three studies each. Future research is needed to determine the effectiveness of TKM treatment in patients with diseases that can cause the main symptoms of wilting disorder.

scholarly journals Western Faculty Profile: Dr. Martin L. Duennwald

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Soojie Hong
Keyword(s):  
Quality Control ◽  
Amyotrophic Lateral Sclerosis ◽  
Protein Misfolding ◽  
Protein Quality ◽  
Assistant Professor ◽  
Cellular Protein ◽  
Independent Research ◽  
Biomedical Research Institute ◽  
Control Protein ◽  
Lateral Sclerosis

Dr. Martin L. Duennwald is a researcher and assistant professor at Western University. After conducting independent research at the Boston Biomedical Research Institute, he came to Western University in 2012 where he started the Duennwald Lab. His lab focuses on cellular protein quality control, protein misfolding and their pathological consequences in neurodegenerative diseases such as Huntington’s disease, Parkinson’s disease, and Amyotrophic Lateral Sclerosis (ALS).

scholarly journals Hochu-Ekki-To Improves Motor Function in an Amyotrophic Lateral Sclerosis Animal Model

Nutrients ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 2644 ◽  
Author(s):  
Mudan Cai ◽  
Eun Jin Yang
Keyword(s):  
Skeletal Muscle ◽  
Spinal Cord ◽  
Amyotrophic Lateral Sclerosis ◽  
Immune System ◽  
Herbal Medicine ◽  
Transgenic Mice ◽  
Motor Neuron ◽  
Motor Function ◽  
Muscle Paralysis ◽  
Lateral Sclerosis

Hochu-ekki-to (Bojungikgi-Tang (BJIGT) in Korea; Bu-Zhong-Yi-Qi Tang in Chinese), a traditional herbal prescription, has been widely used in Asia. Hochu-ekki-to (HET) is used to enhance the immune system in respiratory disorders, improve the nutritional status associated with chronic diseases, enhance the mucosal immune system, and improve learning and memory. Amyotrophic lateral sclerosis (ALS) is pathologically characterized by motor neuron cell death and muscle paralysis, and is an adult-onset motor neuron disease. Several pathological mechanisms of ALS have been reported by clinical and in vitro/in vivo studies using ALS models. However, the underlying mechanisms remain elusive, and the critical pathological target needs to be identified before effective drugs can be developed for patients with ALS. Since ALS is a disease involving both motor neuron death and skeletal muscle paralysis, suitable therapy with optimal treatment effects would involve a motor neuron target combined with a skeletal muscle target. Herbal medicine is effective for complex diseases because it consists of multiple components for multiple targets. Therefore, we investigated the effect of the herbal medicine HET on motor function and survival in hSOD1G93A transgenic mice. HET was orally administered once a day for 6 weeks from the age of 2 months (the pre-symptomatic stage) of hSOD1G93A transgenic mice. We used the rota-rod test and foot printing test to examine motor activity, and Western blotting and H&E staining for evaluation of the effects of HET in the gastrocnemius muscle and lumbar (L4–5) spinal cord of mice. We found that HET treatment dramatically inhibited inflammation and oxidative stress both in the spinal cord and gastrocnemius of hSOD1G93A transgenic mice. Furthermore, HET treatment improved motor function and extended the survival of hSOD1G93A transgenic mice. Our findings suggest that HET treatment may modulate the immune reaction in muscles and neurons to delay disease progression in a model of ALS.

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