Inhibitory Effects of Euphorbia tirucalli Lineu (Euphorbiaceae) Diluted Latex on Human and Canine Melanoma Cells

Euphorbia tirucalli Lineu (Euphorbiaceae) is a tropical and subtropical ornamental and toxic plant. E. tirucalli produces a latex that is commonly used to treat neoplasms. This study aimed to evaluate the effects of diluted E. tirucalli latex (DETL) on human (SK-MEL-28) and canine (CBMY) melanoma cells. SK-MEL-28 (3 × 103 cells/well) and CBMY (6 × 103 cells/well) were cultivated in 96-well plates. The cells were treated with 50 μl/well of dilutions (1/2, 1/4, 1/8, 1/16, 1/32, 1/64, 1/128, 1/256, and 1/512) of a standard solution containing 1 mg/mL of the E. tirucalli latex (ETL) in DMEM. Control group cells received 50 μl/well of DMEM. After 24, 48, and 72 h of treatment, cell viability was assessed by the MTT assay. There was a significant decrease in viability at 48 and 72 hours after treatment for human melanoma cells and at 24, 48, and 72 hours for canine cells, mainly in higher dilutions of ETL. Human melanoma cells presented a typical U shape curve, characteristic of hormesis. To our knowledge, this is the first study showing inhibitory effects of DETL on canine melanoma cells. Therefore, DETL is a potentially new antineoplastic drug.

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Cold atmospheric plasma generated reactive species aided inhibitory effects on human melanoma cells: an in vitro and in silico study

Scientific Reports ◽

10.1038/s41598-020-60356-0 ◽

2020 ◽

Vol 10 (1) ◽

Cited By ~ 4

Author(s):

Dharmendra Kumar Yadav ◽

Manish Adhikari ◽

Surendra Kumar ◽

Bhagirath Ghimire ◽

Ihn Han ◽

...

Keyword(s):

In Silico ◽

Melanoma Cells ◽

Reactive Species ◽

Human Melanoma ◽

Atmospheric Plasma ◽

Inhibitory Effects ◽

Cold Atmospheric Plasma ◽

Human Melanoma Cells ◽

In Silico Study

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Inhibitory Effects of α-Arbutin on Melanin Synthesis in Cultured Human Melanoma Cells and a Three-Dimensional Human Skin Model

Biological and Pharmaceutical Bulletin ◽

10.1248/bpb.27.510 ◽

2004 ◽

Vol 27 (4) ◽

pp. 510-514 ◽

Cited By ~ 80

Author(s):

Kazuhisa Sugimoto ◽

Takahisa Nishimura ◽

Koji Nomura ◽

Kenji Sugimoto ◽

Takashi Kuriki

Keyword(s):

Human Skin ◽

Three Dimensional ◽

Melanoma Cells ◽

Human Melanoma ◽

Melanin Synthesis ◽

Inhibitory Effects ◽

Skin Model ◽

Human Melanoma Cells

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Analysis of in vitro activity of high dilutions of Euphorbia tirucalli L. in human melanoma cells

International Journal of High Dilution Research - ISSN 1982-6206 ◽

10.51910/ijhdr.v10i36.504 ◽

2021 ◽

Vol 10 (36) ◽

pp. 183-193

Author(s):

Renata Macedo dos Reis Januário Da Silva ◽

DulcinÃƒÆ’Ã‚Â©ia Furtado Teixeira ◽

AndrÃƒÆ’Ã‚Â© Luiz Franco Sampaio ◽

Teresa Cristina Andrade Leitao Aguiar

Keyword(s):

Melanoma Cells ◽

Human Melanoma ◽

Maximal Increase ◽

Inhibition Of Proliferation ◽

Popular Medicine ◽

Euphorbia Tirucalli ◽

Homeopathic Medicine ◽

Human Melanoma Cells ◽

Mother Tincture

Aveloz (firestick cactus; Euphorbia tirucalli L.) belongs to Euphorbiaceae family, characterized by the production of a toxic latex that has corrosive effects on the skin and mucous membranes. Continual topic use of the latex is recommended by popular medicine to treat warts, and epitheliomas. To validate this indication, ultra diluted latex and homeopathic medicine Euphorbia tirucalli were tested in vitro on the proliferation of melanoma cells. Ultra diluted latex was prepared in homeopathic dilutions 5cH, 15cH and 30cH by dilution and agitation (trituration for solid and sucussion for liquid phases) using 70Ãƒâ€šÃ‚Âº GL (Gay Lussac) ethylic alcohol (70Ãƒâ€šÃ‚Âº GL EtOH 70Ãƒâ€šÃ‚ÂºGL) as inert medium according to the guidelines in FarmacopÃƒÆ’Ã‚Â©ia Homeopática Brasileira (FHB). Homeopathic medicine Euphorbia tirucalli was prepared from mother-tincture according to the centesimal Hahnemannian method. Solutions 0.5% and 5% of 70Ãƒâ€šÃ‚ÂºGL EtOH were succussed and used as control. Human melanoma cells were cultured, treated and monitored by method MTT for 24 to 72 hours. It was observed that 0.5% 70Ãƒâ€šÃ‚ÂºGL EtOH solution had little or no effect on the proliferation of melanoma cells (5.1% maximal inhibition in dilution 30cH). Positive correlation was observed in most groups between inhibition of proliferation and diluted preparations, maximal increase (9%) was seen in with 5% latex. Moreover, mother-tincture proved to be more active than latex; treatment with 0.5% solution of latex 30cH exhibited 19.7% inhibition, whereas treatment with 0.5% solution of Euphorbia tirucalli 30cH exhibited 32.1% inhibition of cell proliferation (p

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