atmospheric plasma
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2024 ◽  
Vol 84 ◽  
Author(s):  
F. E. P. Diógenes ◽  
S. R. C. Nascimento ◽  
C. Alves Junior ◽  
E. P. Paiva ◽  
S. B. Torres ◽  
...  

Abstract This study aimed to evaluate the effect of atmospheric plasma application on the inactivation of fungi on the surface of Erythrina velutina seeds and on isolated fungal colonies. Two experiments were conducted using a completely randomized design. First, plasma was applied to the surface of the seeds using helium gas and atmospheric plasma for 3, 6, and 9 min in addition to the control (untreated seeds), constituting seven treatments with five repetitions each. In the second experiment, Petri dishes containing the inoculum of different fungi were treated with atmospheric air plasma for 3, 6, and 9 min (Air-3, Air-6, and Air-9) and were compared with untreated fungi in Petri dishes without treatment (control), totaling four treatments and five repetitions each. We found that the application of atmospheric air plasma to E. velutina seeds for 9 min had an antimicrobial effect on the fungi Aspergillus niger, Aspergillus flavus, Fusarium sp., Brachysporium sp., and Rhizopus sp. The formation of fungal colonies isolated from E. velutina seeds was also inhibited by 3 min of exposure to atmospheric air plasma, except for A. niger, whose inhibition occurred after 6 min of exposure to atmospheric plasma.


2022 ◽  
Vol 141 ◽  
pp. 106405
Author(s):  
Pavol Ďurina ◽  
Tomáš Plecenik ◽  
Tomáš Roch ◽  
Veronika Medvecká ◽  
Martin Truchlý ◽  
...  

2022 ◽  
Vol 141 ◽  
pp. 107411
Author(s):  
Q. Dong ◽  
J. Tan ◽  
R. Huang ◽  
H.L. Wang ◽  
P. Song ◽  
...  

2022 ◽  
Vol 11 ◽  
Author(s):  
Christos A. Aggelopoulos ◽  
Anna-Maria Christodoulou ◽  
Myrsini Tachliabouri ◽  
Stauros Meropoulis ◽  
Maria-Elpida Christopoulou ◽  
...  

Breast cancer exists in multiple subtypes some of which still lack a targeted and effective therapy. Cold atmospheric plasma (CAP) has been proposed as an emerging anti-cancer treatment modality. In this study, we investigated the effects of direct and indirect CAP treatment driven by the advantageous nanosecond pulsed discharge on breast cancer cells of different malignant phenotypes and estrogen receptor (ER) status, a major factor in the prognosis and therapeutic management of breast cancer. The main CAP reactive species in liquid (i.e. H2O2, NO2−/NO3−) and gas phase were determined as a function of plasma operational parameters (i.e. treatment time, pulse voltage and frequency), while pre-treatment with the ROS scavenger NAC revealed the impact of ROS in the treatment. CAP treatment induced intense phenotypic changes and apoptosis in both ER+ and ER- cells, which is associated with the mitochondrial pathway as evidenced by the increased Bax/Bcl-2 ratio and cleavage of PARP-1. Interestingly, CAP significantly reduced CD44 protein expression (a major cancer stem cell marker and matrix receptor), while differentially affected the expression of proteases and inflammatory mediators. Collectively, the findings of the present study suggest that CAP suppresses breast cancer cell growth and regulates several effectors of the tumor microenvironment and thus it could represent an efficient therapeutic approach for distinct breast cancer subtypes.


2022 ◽  
Vol 1 (1) ◽  
pp. 41-48
Author(s):  
Anvar Kadirmetov ◽  
Dmitrii Popov ◽  
Stepan Agarkov

The microstructure and phase composition of the coating obtained by plasma spraying of FeCoCrAlTiCuMo powder in an equiatomic ratio of components have been investigated. The results showed the possibility of creating a multicomponent single-phase solid solution by plasma spraying and the expediency of studying it.


2022 ◽  
Vol 11 (2) ◽  
pp. 345-353
Author(s):  
Meiqi Dai ◽  
Xuemei Song ◽  
Chucheng Lin ◽  
Ziwei Liu ◽  
Wei Zheng ◽  
...  

AbstractYttria-stabilized zirconia (YSZ) coatings and Al2O3-YSZ coatings were prepared by atmospheric plasma spraying (APS). Their microstructural changes during thermal cycling were investigated via scanning electron microscopy (SEM) equipped with electron backscatter diffraction (EBSD) and X-ray diffraction (XRD). It was found that the microstructure and microstructure changes of the two coatings were different, including crystallinity, grain orientation, phase, and phase transition. These differences are closely related to the thermal cycle life of the coatings. There is a relationship between crystallinity and crack size. Changes in grain orientation are related to microscopic strain and cracks. Phase transition is the direct cause of coating failure. In this study, the relationship between the changes in the coating microstructure and the thermal cycle life is discussed in detail. The failure mechanism of the coating was comprehensively analyzed from a microscopic perspective.


Materials ◽  
2022 ◽  
Vol 15 (2) ◽  
pp. 496
Author(s):  
Lisa Krautwald ◽  
Ralf Smeets ◽  
Carolin Stolzer ◽  
Rico Rutkowski ◽  
Linna Guo ◽  
...  

The influence of UV light and non-thermal plasma on the osseointegration of yttria-stabilized zirconia implants (Y-TZP) comparing the two methods is unclear. The aim of this study was to show the influence of these methods on the osseointegration of dental zirconia implants in an animal model. A total of 54 implants were either untreated, treated with UV light (UV), or non-thermal oxygen plasma for 12 min and inserted into the parietal bones of six domestic pigs. The animals were sacrificed after a healing interval of two, four, and nine weeks. The degree of osseointegration was determined using histomorphometric determination of bone-to-implant contact values (BIC) and the bone-to-implant contact values within the retentive parts of the implants (BAFO). BIC values decreased in all groups after four weeks of healing and re-increased after nine weeks in all groups. BAFO increased significantly over time in all groups. However, there were no statistically significant differences in BIC and BAFO values between the control group and the test groups and over time. Clinical studies may follow to confirm the influence of cold plasma and UV light on the healing and survival of zirconia implants.


2022 ◽  
Author(s):  
Jonas Hiller ◽  
Bernd Stratmann ◽  
Jürgen Timm ◽  
Tania‐Cristina Costea ◽  
Diethelm Tschoepe

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Osvaldo Daniel Cortázar ◽  
Ana Megía-Macías ◽  
Sandra Moreno ◽  
Alejandro Brun ◽  
Eduardo Gómez-Casado

AbstractCold Atmospheric Plasma (CAP) and Plasma Activated Media (PAM) are effective against bacteria, fungi, cancer cells, and viruses because they can deliver Reactive Oxygen and Nitrogen Species (RONS) on a living tissue with negligible damage on health cells. The antiviral activity of CAP against SARS-CoV-2 is being investigated, however, the same but of PAM has not been explored despite its potential. In the present study, the capability of Plasma Activated Media (PAM) to inactivate SARS-CoV-2 and PR8 H1N1 influenza virus with negligible damage on healthy cells is demonstrated. PAM acted by both virus detaching and diminished replication. Furthermore, the treatment of A549 lung cells at different times with buffered PAM did not induce interleukin 8 expression, showing that PAM did not induce inflammation. These results open a new research field by using PAM to the development novel treatments for COVID-19, influenza, and other respiratory diseases.


2022 ◽  
Vol 12 (2) ◽  
pp. 590
Author(s):  
Bogdan Caba ◽  
Ioannis Gardikiotis ◽  
Ionut Topala ◽  
Ilarion Mihaila ◽  
Cosmin Teodor Mihai ◽  
...  

The evolution of reconstructive methods for defects of the human body cannot yet replace the use of flap surgery. Research is still preoccupied with the ideal techniques for offering the best chances of survival of the flaps. In our study, we investigated the effects of cold atmospheric plasma (CAP), N-nitro-L-arginine methyl ester (L-NAME), and platelet-rich plasma (PRP) injectable solutions on flap survival using an in vivo model. Twenty-four Wistar rats (four groups) had the McFarlane flap raised and CAP, L-NAME, and PRP substances tested through a single dose subcutaneous injection. The control group had only a saline solution injected. To the best of our knowledge, this is the first study that evaluated a CAP activated solution through injection on flaps. The flap survival rate was determined by clinical examination (photography documented), hematology, thermography, and anatomopathological tests. The image digital analysis performed on the flaps showed that the necrosis area (control—49.64%) was significantly lower for the groups with the three investigated solutions: CAP (14.47%), L-NAME (18.2%), and PRP (23.85%). Thermography exploration revealed less ischemia than the control group on the CAP, L-NAME, and PRP groups as well. Anatomopathological data noted the best degree of angiogenesis on the CAP group, with similar findings on the L-NAME and PRP treated flaps. The blood work did not indicate infection or a strong inflammatory process in any of the subjects. Overall, the study shows that the CAP activated solution has a similar (better) impact on the necrosis rate (compared with other solutions with known effects) when injected on the modified dorsal rat skin flap, and on top of that it can be obtained fast, in unlimited quantities, non-invasively, and through a standardized process.


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