scholarly journals Effect of Warm Acupuncture Combined with Bone Marrow Mesenchymal Stem Cells Transplantation on Cartilage Tissue in Rabbit Knee Osteoarthritis

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Jun-wei Liu ◽  
Yong-li Wu ◽  
Wei Wei ◽  
Yan-ling Zhang ◽  
Di Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Knee Joint ◽  
Knee Osteoarthritis ◽  
Cartilage Tissue ◽  
Blue Staining ◽  
Chondrocyte Apoptosis ◽  
Rabbit Knee ◽  
Marrow Mesenchymal Stem Cells

The current study was designed to investigate the effect and underlying mechanism of warm acupuncture combined with bone marrow mesenchymal stem cells (BMSC) transplantation on cartilage tissue injury in rabbit knee osteoarthritis (KOA). In the study, 50 rabbits were randomly divided into 5 groups: blank group, KOA group, warm acupuncture group, BMSCs group, and warm acupuncture combined with BMSCs group. After warm acupuncture combined with BMSCs, the Modified Lequesne MG knee joint assessment scale was used to evaluate the degree of knee joint behavior, the Taiping Peng method generally observed the histomorphology changes of KOA rabbit cartilage, and hematoxylin-eosin staining, safranin O green staining, and toluidine blue staining were conducted to evaluate the extent of cartilage tissue pathology. Furthermore, transmission electron microscopy and TUNEL staining were used to observe cell apoptosis, and immunohistochemistry and qPCR analysis were used to detect the expression of apoptosis-related proteins and mRNA. Results showed that administration of warm acupuncture combined with BMSCs recovered the joint function and significantly decreased Lequesne MG score. The degree of cartilage tissue pathological damage has been improved, cartilage ultrastructure degeneration has recovered, peripheral blood vessels have mild edema, blood supply has gradually recovered, and even small amounts of red blood cells have appeared. In addition, warm acupuncture combined with BMSCs treatment suppressed chondrocyte apoptosis in rabbits with knee osteoarthritis by reduced TUNEL-positive chondrocytes and simultaneously reversed the mRNA expression of Bax, Bcl-2, and Caspase-3. These results indicate that warm acupuncture combined with BMSCs transplantation has a potential protective effect on rabbit KOA, which may be mediated by inhibiting chondrocyte apoptosis.

Treatment of Knee Osteoarthritis With Allogeneic Bone Marrow Mesenchymal Stem Cells

2015 ◽  
Vol 99 (8) ◽  
pp. 1681-1690 ◽  
Author(s):  
Aurelio Vega ◽  
Miguel Angel Martín-Ferrero ◽  
Francisco Del Canto ◽  
Mercedes Alberca ◽  
Veronica García ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Knee Osteoarthritis ◽  
Allogeneic Bone Marrow ◽  
Allogeneic Bone ◽  
Marrow Mesenchymal Stem Cells

ALK5 transfection of bone marrow mesenchymal stem cells to repair osteoarthritis of knee joint

2018 ◽  
Vol 1 (2) ◽  
pp. 135-145 ◽  
Author(s):  
Danna Cao ◽  
Liang Ma ◽  
Xiaodong Han ◽  
Lingqing Dong ◽  
Mengfei Yu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Knee Joint ◽  
Osteoarthritis Of Knee ◽  
Marrow Mesenchymal Stem Cells

scholarly journals Flt3 ligand expanded bone marrow mesenchymal stem cells for cartilage tissue engineering

Cell Research ◽  
2008 ◽  
Vol 18 (S1) ◽  
pp. S56-S56 ◽  
Author(s):  
Chang M Guo ◽  
Kelly YW Chan ◽  
Philip Cheang ◽  
Kam M Hui ◽  
Ivy AW Ho ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Flt3 Ligand ◽  
Marrow Mesenchymal Stem Cells

Overexpressing Runx2 of BMSCs Improves the Repairment of knee Cartilage Defects

2020 ◽  
Vol 20 (5) ◽  
pp. 395-404
Author(s):  
Jing Hu ◽  
Wen-Zhong Zou ◽  
Ling Li ◽  
Zheng-shuai Shi ◽  
Xiang-Zhong Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Articular Cartilage ◽  
Cartilage Repair ◽  
Joint Fluid ◽  
Cartilage Tissue ◽  
Cell Group ◽  
Knee Cartilage ◽  
Marrow Mesenchymal Stem Cells

Background: Recruitment of gene modifying bone marrow mesenchymal stem cells (BMSCs) has been considered an alternative to single-cell injection in articular cartilage repair. Purpose: This study aimed to investigate whether the effect of runt-related transcription factor 2(Runx2) overexpression bone marrow mesenchymal stem cells in vivo could improve the quality of repaired tissue of a knee cartilage defect in a rabbit model. Methods: Thirty-two New Zealand rabbits were randomly divided into four groups. The blank group (Con) did not receive anything, the model group (Mo) was administered saline, the simple stem cell group (MSCs) received MSCs injection, and the Runx2 transfection group (R-MSCs) received Runx2 overexpression MSCs injection. After adapting to the environment for a week, a 5 mm diameter cylindrical osteochondral defect was created in the center of the medial femoral condyle. Cell and saline injections were performed in the first and third weeks after surgery. The cartilage repair was evaluated by macroscopically and microscopically at 4 and 8 weeks. Results: Macroscopically, defects were filled and surfaces were smoother in the MSCs groups than in the Mo group at 4th week. Microscopically, the R-MSCs group showed coloration similar to surrounding normal articular cartilage tissue at 8 weeks in masson trichrome staining. The COL-II, SOX9, and Aggrecan mRNA expressions of MSCs were enhanced at 4 weeks compared with R-MSCs, then the expression reduced at 8 weeks, but was still higher than Mo group level (P<0.05). The western blot examination revealed that the COL-IIand SOX9 expression of MSCs was higher than R-MSCs at 4 weeks, then the expression reduced at 8 weeks, but was still higher than the Mo level (P<0.05). The IL-1β content in the joint fluid also revealed that cartilage repair with R-MSCs was better than that with MSCs at 8 weeks (P<0.05). Conclusions: The R-MSCs group showed cellular morphology and arrangement similar to surrounding normal articular cartilage tissue, and Runx2 overexpression of MSCs resulted in overall superior cartilage repair as compared with MSCs at 8 weeks.

scholarly journals Over-Confluence of expanded bone marrow mesenchymal stem cells ameliorates their chondrogenic capacity in 3D cartilage tissue engineering

2020 ◽  
Author(s):  
Damien Tucker ◽  
Karen Still ◽  
Ashley Blom ◽  
Anthony P. Hollander ◽  
Wael Kafienah
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Perceived Risk ◽  
Cartilage Tissue Engineering ◽  
Culture Technique ◽  
Cartilage Tissue ◽  
Differentiation Potential ◽  
Marrow Mesenchymal Stem Cells

ABSTRACTCartilage tissue engineering using bone marrow-derived mesenchymal stem cells (BM-MSCs) is a growing technology for the repair of joint defects. Culturing BM-MSCs to over confluence has historically been avoided due to perceived risk to cell viability, growth inhibition and differentiation potential. Here we show that a simple change in culture practice, based on mimicking the condensation phase during embryonic cartilage development, results in biochemically and histologically superior cartilage tissue engineered constructs. Whole transcriptome analysis of the condensing cells revealed a phenotype associated with early commitment to chondrogenic precursors. This simple adjustment to the common stem cell culture technique would impact the quality of all cartilage tissue engineering modalities utilising these cells.

Sign in / Sign up

Export Citation Format

Share Document