scholarly journals Dynamic Changes of Urine Proteome in Rat Models Inoculated with Two Different Hepatoma Cell Lines

2021 ◽  
Vol 2021 ◽  
pp. 1-20
Author(s):  
Yameng Zhang ◽  
Yufei Gao ◽  
Jing Wei ◽  
Youhe Gao
Keyword(s):  
Cell Lines ◽  
Hepatoma Cell ◽  
Tumor Model ◽  
Differentially Expressed ◽  
Urinary Proteins ◽  
Protein Patterns ◽  
Rat Models ◽  
Urine Proteome ◽  
Hepatoma Cell Lines ◽  
Histological Results

Urine can accumulate systemic changes with no mechanism to be stable, which may reflect early changes associated with physiological or pathophysiological processes. To explore the potential value of the urine proteome, two rat models were established by intrahepatic injection of two different hepatoma cell lines, CBRH-7919 and RH-35. Urine samples were collected and analyzed. Compared with controls, the two models exhibited different numbers and types of differentially expressed urinary proteins despite having similar histological results. The results were compared with the urine proteome of a Walker 256 (W-256) liver tumor model. The differentially expressed urinary protein patterns in the three models were different. These findings demonstrate that changes in the urine proteomes of the two models can be detected at early stages and that the patterns of differentially expressed urinary proteins can differ even when the histological results are similar. Urinary proteins have potential utility for distinguishing among different tumor cells grown in the same organ.

scholarly journals Multiplexed Proteomic Approach for Identification of Serum Biomarkers in Hepatocellular Carcinoma Patients with Normal AFP

2020 ◽  
Vol 9 (2) ◽  
pp. 323
Author(s):  
Young-Sun Lee ◽  
Eunjung Ko ◽  
Eileen L. Yoon ◽  
Young Kul Jung ◽  
Ji Hoon Kim ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Cirrhosis ◽  
Cell Lines ◽  
Cellular Proliferation ◽  
Hepatoma Cell ◽  
Multiple Reaction Monitoring ◽  
Serum Levels ◽  
Human Hepatoma Cell ◽  
Hepatoma Cell Lines ◽  
Proteomic Approach

Alpha fetoprotein (AFP) has been used as a serologic indicator of hepatocellular carcinoma (HCC). We aimed to identify an HCC-specific serum biomarker for diagnosis using a multiplexed proteomic technique in HCC patients with normal AFP levels. A total of 152 patients were included from Guro Hospital, Korea University. Among 267 identified proteins, 28 and 86 proteins showed at least a two-fold elevation or reduction in expression, respectively. Multiple reaction monitoring (MRM) analysis of 41 proteins revealed 10 proteins were differentially expressed in patients with liver cirrhosis and HCC patients with normal AFP. A combination of tripartite motif22 (Trim22), seprase, and bone morphogenetic protein1 had an area under receiver operating characteristic of 0.957 for HCC diagnosis. Real-time PCR and western blot analysis of the paired tumor/non-tumor liver tissue in HCC revealed a reduced expression of Trim22 in the tumor tissue. Also, serum levels of Trim22 were significantly reduced in HCC patients with normal AFP compared to those with liver cirrhosis (p = 0.032). Inhibition of Trim22 increased cellular proliferation in human hepatoma cell lines, whereas overexpression of Trim22 decreased cellular proliferation in hepatoma cell lines. In conclusion, the combination of three serum markers improved the chance of diagnosing HCC. MRM-based quantification of the serum protein in patients with normal AFP provides the potential for early diagnosis of HCC.

scholarly journals The Limonoids TS3 and Rubescin E Induce Apoptosis in Human Hepatoma Cell Lines and Interfere with NF-κB Signaling

PLoS ONE ◽  
2016 ◽  
Vol 11 (8) ◽  
pp. e0160843 ◽  
Author(s):  
Nicole Lange ◽  
Armelle Tsamo Tontsa ◽  
Claudia Wegscheid ◽  
Pierre Mkounga ◽  
Augustin Ephrem Nkengfack ◽  
...  
Keyword(s):  
Cell Lines ◽  
Hepatoma Cell ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Hepatoma Cell Lines

Significant decrease in telomerase activity during differentiation of human hepatoma cell lines

1998 ◽  
Vol 114 ◽  
pp. A1309 ◽  
Author(s):  
M. Nakamura ◽  
H. Saito ◽  
H. Ebinuma ◽  
M. Takahashi ◽  
F. Kaneko ◽  
...  
Keyword(s):  
Cell Lines ◽  
Hepatoma Cell ◽  
Telomerase Activity ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Hepatoma Cell Lines

Expression of HIP/PAP mRNA in Human Hepatoma Cell Lines

2006 ◽  
Vol 963 (1) ◽  
pp. 53-58 ◽  
Author(s):  
M. CERVELLO ◽  
L. GIANNITRAPANI ◽  
M. ROSA ◽  
M. NOTARBARTOLO ◽  
N. D'ALESSANDRO ◽  
...  
Keyword(s):  
Cell Lines ◽  
Hepatoma Cell ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Hepatoma Cell Lines
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