DNA Damage–Induced BARD1 Phosphorylation Is Critical for the Inhibition of Messenger RNA Processing by BRCA1/BARD1 Complex

(R)-Goniothalamin (R-GNT) is a secondary metabolite isolated from the plants of the genus Goniothalamus. This molecule has attracted the attention of researchers because of its selective cytotoxicity against tumor cells and its ability to induce apoptosis. (S)-Goniothalamin (S-GNT) is a synthetic enantiomer of R-GNT, and its mechanism of action is largely unknown. In this study, we investigated the activity of S-GNT in a human non-small cell lung cancer NCI-H460 cells. We observed that the cells exposed to this compound exhibited cytotoxicity in a concentration-dependent manner. Based on the data obtained through the assessment of apoptosis induction in situ and the comet assay, we suggest that this cytotoxicity occurs due to the potential ability of this molecule to induce DNA damage with the consequent induction of cell death via apoptosis. A significant reduction in the messenger RNA levels of baculoviral inhibitor of apoptosis repeat-containing 5 ( BIRC5) gene that encodes the survivin protein was found. This novel finding may explain the inhibition of cell proliferation and induction of apoptosis in tumor cells caused by this compound.

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The RNA Processing Factor Y14 Participates in DNA Damage Response and Repair

iScience ◽

10.1016/j.isci.2019.03.005 ◽

2019 ◽

Vol 13 ◽

pp. 402-415 ◽

Cited By ~ 2

Author(s):

Tzu-Wei Chuang ◽

Chia-Chen Lu ◽

Chun-Hao Su ◽

Pei-Yu Wu ◽

Sarasvathi Easwvaran ◽

...

Keyword(s):

Dna Damage ◽

Dna Damage Response ◽

Rna Processing ◽

Processing Factor ◽

Damage Response

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The Interplay Between the DNA Damage Response, RNA Processing and Extracellular Vesicles

Frontiers in Oncology ◽

10.3389/fonc.2019.01538 ◽

2020 ◽

Vol 9 ◽

Author(s):

Xiangbing Meng ◽

Shujie Yang ◽

Vanessa J. A. Camp

Keyword(s):

Dna Damage ◽

Extracellular Vesicles ◽

Dna Damage Response ◽

Rna Processing ◽

Damage Response

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RNA Interference Protects Against 5-Lipoxygenase-Induced Cocarcinogen, Benzidine, Oxidation and Cytotoxicity in Human Tracheobronchial Epithelial Cells

International Journal of Toxicology ◽

10.1177/1091581814542030 ◽

2014 ◽

Vol 33 (4) ◽

pp. 297-306 ◽

Cited By ~ 2

Author(s):

Yun Huang ◽

Qingping Tan ◽

Yue Wu ◽

Hongxiang Zhu ◽

Minru Xiong ◽

...

Keyword(s):

Dna Damage ◽

Rna Interference ◽

Epithelial Cells ◽

Messenger Rna ◽

Specific Inhibitor ◽

Hairpin Rna ◽

Oxidative Activation ◽

Or Gene ◽

Rna Knockdown

Lipoxygenase (LOX)-catalyzed cooxidation of the human carcinogen benzidine (BZD) has been shown in in vitro enzyme systems. This study aimed to determine whether BZD could be activated by arachidonate 5-lipoxygenase ( ALOX5) in the human tracheobronchial epithelial cells (HBECs) using RNA interference strategy and a 5-LOX-specific inhibitor, AA861. We show that the soybean LOX catalyzed the cooxidation of BZD, generating BZD diimine. Benzidine induced expression of ALOX5 messenger RNA and 5-LOX protein in HBECs, and significantly decreased cell proliferation, but enhanced DNA damage and apoptosis in HBECs which were significantly inhibited by lentiviral-mediated small hairpin RNA-knockdown of ALOX5 and by AA861. Thus, BZD could upregulate the expression of ALOX5 in HBECs, while inhibition of the protein or gene expression or enzyme activity could prevent BZD-induced cytotoxicity and DNA damage in HBECs, which might be caused by the 5-LOX-catalyzed oxidative activation of BZD.

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Nuclear RNA-protein interactions and messenger RNA processing.

The Journal of Cell Biology ◽

10.1083/jcb.97.5.1321 ◽

1983 ◽

Vol 97 (5) ◽

pp. 1321-1326 ◽

Cited By ~ 32

Author(s):

T Pederson

Keyword(s):

Detailed Analysis ◽

Recent Work ◽

Protein Interactions ◽

Rna Processing ◽

Messenger Rna ◽

Mammalian Cells ◽

General Hypothesis ◽

Ribonucleoprotein Particles ◽

Nuclear Rna ◽

Nuclear Ribonucleoprotein

Eucaryotic messenger RNA precursors are processed in nuclear ribonucleoprotein particles (hnRNP). Here recent work on the structure of hnRNP is reviewed, with emphasis on function. Detailed analysis of a specific case, the altered assembly of hnRNP in heat-shocked Drosophila and mammalian cells, leads to a general hypothesis linking hnRNP structure and messenger RNA processing.

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