Abstract P1-07-02: Mesenchymal stem cell regulated microRNAs converge on the speech gene FOXP2 and regulate breast cancer metastasis

Epithelial-mesenchymal transition (EMT) is a multistep process in which cells acquire molecular alterations such as loss of cell-cell junctions and restructuring of the cytoskeleton. There is an increasing understanding that this process may promote breast cancer progression through promotion of invasive and metastatic tumor growth. Recent observations imply that there may be a cross-talk between EMT and cancer stem cell properties, leading to enhanced tumorigenicity and the capacity to generate heterogeneous tumor cell populations. Here, we review the experimental and clinical evidence for the involvement of EMT in cancer stem cell theory, focusing on the common characteristics of this phenomenon.

Download Full-text

Effect of tetrandrine combined with arsenic trioxide on stem cells of triple negative breast cancer

10.21203/rs.2.10175/v1 ◽

2019 ◽

Author(s):

Yubo Guo ◽

Yingyi Fan ◽

Ruixian Wu ◽

Xiaohua Pei

Keyword(s):

Breast Cancer ◽

Stem Cells ◽

Flow Cytometry ◽

Stem Cell ◽

Arsenic Trioxide ◽

Triple Negative Breast Cancer ◽

Cancer Metastasis ◽

Triple Negative ◽

Breast Cancer Metastasis ◽

Blue Staining

Abstract Background Although the triple negative breast cancer is sensitive to chemotherapy, breast cancer stem cells (BCSCs) is the origin of tumor chemotherapy resistance, tumor recurrence and tumor invasion and metastasis. This study aims to examine the effect of tetrandrine combine with arsenic trioxide on BCSCs and potential mechanism of anti- triple negative breast cancer metastasis. Methods We cultured the triple negative breast cancer cell MDA-MB-231 and induced BCSCs sphere formation by serum-free medium for 5 days. In the MDA-MB-231 cell and MDA-MB-231 stem cell, we compared the ratio of CD44+/CD24- and sorted stem cells by flow cytometry, the expression of Oct4 and Sox2mRNA were by rt-PCR, invasion ability were by Transwell assay. We subsequently measured the effect of tetrandrine combine with arsenic trioxide on BCSCs proliferation by CCK8 method. The stem cell morphology observation was by trypan blue staining. Stem cell cycle and apoptosis were evaluated by flow cytometry. Western Blot was used to measure the protein levels of Hedgehog, Notch1 and PTEN signaling of BCSCs. Results The ratio of CD44+/CD24- in MDA-MB-231 stem cells was 95.0%, while MDA-MB-231 cell was 89.3%. The invasion number of MDA-MB-231 stem cell was significantly higher than that of MDA-MB-231 cells (p<0.01). Furthermore, we demonstrated that tetrandrine and arsenic trioxide could inhibit the BCSCs proliferation. Tetrandrine combine with arsenic trioxide could significantly promote the apoptosis (p<0.01) and increase the percentage of G0/G1 phase and decrease the G2/M phase (p<0.01) of BCSCs. Compared with the control group, arsenic trioxide, tetrandrine and the combined group could significantly reduce the expression of GLI1 and SMO and increase the expression of PTEN protein (P<0.05). Conclusions These findings revealed that tetrandrine combined with arsenic trioxide could suppress the proliferation and induce apoptosis of BCSCs by decreased Gli and SMO expression and increased PTEN expression. Targeting BCSCs treatment, this study provides potential therapeutic drugs against triple negative breast cancer metastasis.

Download Full-text

Abstract P1-07-30: FOXC1/FOXA1 transcriptional balance in breast cancer: From acquisition of mesenchymal and stem cell traits to occult lymph node independent breast cancer metastasis

10.1158/1538-7445.sabcs14-p1-07-30 ◽

2015 ◽

Author(s):

Partha S Ray ◽

Tor W Jensen ◽

Tania Ray ◽

Omid Gholamalamdari ◽

Connie Y Tsai ◽

...

Keyword(s):

Breast Cancer ◽

Stem Cell ◽

Lymph Node ◽

Cancer Metastasis ◽

Breast Cancer Metastasis

Download Full-text

Potential targeted therapy: The role of MicroRNAs in breast cancer metastasis via epithelial-mesenchymal transition and cancer stem cell regulation

Indonesian Journal of Pharmacology and Therapy ◽

10.22146/ijpther.1184 ◽

2021 ◽

Vol 2 (1) ◽

Author(s):

Pamungkas Bagus Satriyo

Keyword(s):

Breast Cancer ◽

Stem Cell ◽

Targeted Therapy ◽

Cancer Stem Cell ◽

Cancer Treatment ◽

Cancer Metastasis ◽

Metastatic Breast ◽

Breast Cancer Metastasis ◽

Mesenchymal Transition ◽

Junction Protein

Abstract In the advancement of breast cancer treatment, metastatic breast cancer is remaining as an incurable disease. It contributes to almost 90% of cancer-related death in breast cancer cases. Epithelial to Mesenchymal Transition (EMT) is a serial change of the epithelial cell to gain the mesenchymal-like phenotype. In cancer, the cells that undergo the EMT lose the adherent junction protein, cell polarity, and gain the invasive phenotype. Recent studies showed that the EMT induces the cancer stem cell-like phenotypes in cancer cells. These cells possess self-renewal ability, and multi-lineage differentiation capacity to generate the new bulk of tumor during cancer distant metastasis. Both EMT and cancer stem cells take responsibility in drug-resistant, and relapse cases in breast cancer. In the last decades, a new type of non-coding RNA, microRNA (miR) shows have an important role in the normal physiological and pathophysiological condition such as cancer. Recent studies revealed that the EMT is regulated by microRNAs. In this review, we discussed the microRNAs regulation on the EMT process through TGF-β, and Wnt signaling pathways in breast cancer. Understanding of microRNA regulation in EMT in breast cancer metastasis gives a chance to explore a new therapy approach to improve the prognosis of breast cancer patients. In addition, we also explored several potential approaches targeting microRNA as a new approach of cancer treatment. Keywords: breast cancer, microRNA, EMT, metastasis, targeted therapy.

Download Full-text