Abstract A118: Optimization of end point PCR for the determination of ESR1, PGR, and ERBB2 expression level in resource-limited settings: Ethiopian context

2020 ◽  
Author(s):  
Zelalem Desalegn Woldesonbet ◽  
Martina Vetter ◽  
Meron Yohannes Nigussie ◽  
Tamrat Abebe Zeleke ◽  
Yonas Bekuretsion ◽  
...  
Keyword(s):  
Expression Level ◽  
Resource Limited Settings ◽  
End Point ◽  
Erbb2 Expression ◽  
Resource Limited

scholarly journals Evaluation of an immunoassay for determination of plasma efavirenz concentrations in resource-limited settings

2014 ◽  
Vol 17 (1) ◽  
pp. 18979 ◽  
Author(s):  
Alemseged Abdissa ◽  
Lubbe Wiesner ◽  
Helen McIlleron ◽  
Henrik Friis ◽  
Åse Bengård Andersen ◽  
...  
Keyword(s):  
Resource Limited Settings ◽  
Resource Limited

scholarly journals Determination of Serum Carbamazepine Concentration Using Dried Blood Spot Specimens for Resource Limited Settings

2016 ◽  
Vol 19 (7) ◽  
pp. A874 ◽  
Author(s):  
S Das ◽  
DH Fleming ◽  
BS Mathew ◽  
A B Winston ◽  
AT Prabhakar ◽  
...  
Keyword(s):  
Dried Blood Spot ◽  
Resource Limited Settings ◽  
Resource Limited ◽  
Carbamazepine Concentration ◽  
Blood Spot

scholarly journals Evaluation of Photosensitive Paper Coatings as Detectors for Instrumentation-Free UV Photometric Analysis Based on Photography-Based Photometry

Chemosensors ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 233
Author(s):  
Tatiana G. Choleva ◽  
Afroditi Sfakianaki ◽  
Athanasios G. Vlessidis ◽  
Dimosthenis L. Giokas
Keyword(s):  
Uv Irradiation ◽  
Resource Limited Settings ◽  
Photometric Analysis ◽  
Paper Surface ◽  
Real Samples ◽  
Paper Coatings ◽  
Iron Salts ◽  
Resource Limited ◽  
Better Than

Photography-based photometry is a technique developed to perform high throughput UV photometric analysis without instrumental detectors in resource-limited settings. Its principle relies on the illumination of a sample with UV irradiation and then capturing the transmitted irradiation on a photosensitive paper surface. Therefore, the photosensitive surface acts as a detector for the determination of the concentration of analytes in the sample. This work aims to investigate the optimum photosensitive paper coatings for capturing the transmitted UV irradiation. To this end, photosensitive coatings based on silver, iron, and dichromate salts were tested using three assays of pharmaceutical and biochemical interest. The results from both calibrations, using standard solutions and the application in real samples, show that photosensitive coatings based on iron salts provide the best results. Importantly, the detection limits and the linear range of the calibration curves were better than those obtained with standard photometry. Based on these findings, cyanotype green papers, are proposed as optimum detectors for photography-based photometry. This finding simplifies the operation of the technique enabling the fabrication of prototype readers for analytical assays performed in resource limited settings, point-of-need applications or in the field.

Determination of serum carbamazepine concentration using dried blood spot specimens for resource-limited settings

2017 ◽  
Vol 45 (2) ◽  
pp. 46-50 ◽  
Author(s):  
Saibal Das ◽  
Denise H Fleming ◽  
Binu S Mathew ◽  
Blessed Winston A ◽  
Appaswamy T Prabhakar ◽  
...  
Keyword(s):  
Dried Blood Spot ◽  
Resource Limited Settings ◽  
Resource Limited ◽  
Carbamazepine Concentration ◽  
Blood Spot

Potential predictive marker of treatment effectiveness in patients with osteopenic syndrome after surgical menopause

GYNECOLOGY ◽  
2020 ◽  
Vol 22 (4) ◽  
pp. 39-42
Author(s):  
Yansiiat Z. Zaydieva ◽  
Elena V. Kruchinina ◽  
Olga S. Gorenkova ◽  
Elena Yu. Polyakova ◽  
Elena N. Kareva ◽  
...  
Keyword(s):  
Vitamin D ◽  
Combination Therapy ◽  
Vitamin D Receptor ◽  
Surrogate Marker ◽  
Predictive Marker ◽  
Expression Level ◽  
Mineral Density ◽  
Vitamin D Receptors ◽  
Surgical Menopause

Introduction. Patients with surgical menopause have a risk for osteopenic syndrome (OS). Menopausal hormone therapy (MHT) in combination with calcium and vitamin D promotes increase in bone mineral density (BMD). The expression level of vitamin D receptor in mononuclear fraction cells (MNFC) of blood can be considered as a predictive marker of effectiveness of OS therapy. Aim. To search a molecular predictive marker of the effectiveness of OS treatment. Materials and methods. The study included 100 women aged 4055 years with a duration of surgical menopause from 12 months to 6 years. The criterion for including patients in the study was the absence of contraindications to the use of MHT. The subject of the study was the determination of BMD by dual-energy X-ray absorptiometry, polymerase chain reaction diagnostics of the level of expression of vitamin D genes, estradiol and progesterone receptors, determination of 25-OH vitamin D in the blood. Results. Analysis of 12-month OS therapy effectiveness evaluated with a surrogate marker BMD. The increase in BMD up to 34% per year was treated as absence of negative dynamics, more than 4% per year as positive one. Significant effect of combination therapy compared with MHT on BMD in patients with surgical menopause with a low baseline level of BMD (due to hypovitaminosis D) is associated with the anti-inflammatory, bone-protective effect of vitamin D. In both groups of patients not responding; to the prescribed therapy we were able to conduct a comparative analysis of expression level of the target molecules in the MNFC before the start of treatment. The efficacy of MHT and combination therapy for BMD disorders is positively associated with the expression level of vitamin D receptors in MNFC before treatment. Therefore, the vitDR mRNA level is a potential predictive marker of the effectiveness of OS treatment. The expression levels of nuclear estradiol beta receptor and membrane receptor for progesterone in MNFC before treatment showed an upward trend in women responding to therapy. Conclusion. The expression level of the vitamin D receptor in MNFC of blood is significantly lower in the group of women with no/insufficient effect on 12-month combined therapy. This indicator can be considered as a predictive marker of the effectiveness of OS therapy.

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