Abstract IA-07: Genetic ancestry and lung cancer somatic mutations in patients of Latin American descent: Etiology and treatment implications

Background. Great success has been made in the targeting therapy of advanced non-small cell lung cancer (NSCLC). Nowadays, next generation sequencing (NGS) is acquirable and affordable in developed area of China. Using this feasible and accurate method of detecting therapeutic genes would help to select optimal treatments to extend patients survival. Here, we identified somatic mutations by NGS and analyzed the value for treatment of NSCLC in a real-world clinical setting. Methods. NGS was carried out on biopsy samples obtained from 66 advanced unresectable NSCLC patients who had not received any treatment. 23 patients received liquid biopsy after failure of first-line targeted treatment. The mutation profiling as well as associations between mutations and clinicopathological characters was analyzed. The study also assessed the values of NGS for choosing treatment options and predicting prognosis in NSCLC patients. Results. 152 somatic mutations were identified in 45 (68.18%) tissue samples. The most frequently mutated genes were EGFR (42.42%), TP53 (31.82%) and KRAS (15.15%). Specifically, the most frequent EGFR mutation subtypes were exon 19 deletion (60.71%) and L858R in exon 21 (46.43%). 83.33% mutated patients received targeted therapy. Among the adenocarcinoma cases, patients with EGFR exon 19 deletion mutation have longer overall survival (OS) than the wide-type (36.0 months versus 19.0 months p=0.046). In addition, in the smoking group, patients with EGFR exon 19 deletion mutation tended to have longer OS (38.0 months versus 16.5 months p<0.01). After the failure of first-line targeted therapy, 23 EGFR mutated patients received liquid biopsy, and the positive rate of T790M mutation in EGFR exon 20 was 47.83%. T790M positive patients have longer progression-free survival (PFS) than the others (15 months versus 9.5 months p=0.025). Conclusions. The observational study from real-world demonstrated that using NGS in routine clinical detection may be useful in guiding the therapy decisions and benefit more Chinese NSCLC patients.

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Epidermal growth factor mutation in non-small cell lung cancer: Venezuelan experience.

Journal of Clinical Oncology ◽

10.1200/jco.2013.31.15_suppl.e22060 ◽

2013 ◽

Vol 31 (15_suppl) ◽

pp. e22060-e22060

Author(s):

George Oblitas Alca ◽

Maria Belen Fuentes ◽

Juan Jose Jose Rodriguez ◽

Cristina Garcia ◽

Maria Angelina Perez ◽

...

Keyword(s):

Lung Cancer ◽

Somatic Mutations ◽

Egfr Mutation ◽

Current Treatment ◽

Male Gender ◽

Small Cell Lung ◽

Demographic Behavior ◽

Current Treatment Strategy ◽

Epidermal Growth ◽

Nsclc Patients

e22060 Background: Lung cancer represents the most common cause of death related to cancer worldwide. The current treatment strategy includes the determinations of the EGFR somatic mutations. Currently in Venezuela the frequency and the type of EGFR mutation in NSCLC patients is unknown and due to its importance in the decision of treatment, it was decided to execute this study with the objective of knowing our population’s characteristics with respect to the stage and mutational type of EGFR. Methods: In the period between November of 2010 until March of 2012, the samples of 395 patients with NSCLC diagnostic all over the country were obtained, these samples were evaluated to determine the EGFR mutation, confidence intervals of 95% were calculated based on the binomial distribution. Results: From the total of 395 patients, 80 presented an inadequate sample, 19 had no registry, 296 patients remained for the analysis. It was obtained an EGFR mutation rate of 11.5%, that was more common seen in male gender (56%), younger than 65 years (66%), ex smokers (59%), with Adenocarcinoma histology (97%). About the mutation type of the EGFR, the specific mutation of the exon 21 was found in 59% of the positive cases, and deletion of exon 19 in 38%. Conclusions: The EGFR mutation is present within the Venezuelan population and shows a particular regional demographic behavior.

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