Patch Clamp Recordings of Single Ion Channel Activation by Gonadotrophin-Releasing Hormone in Ovine Pituitary Gonadotrophs

1986 ◽  
Vol 43 (2) ◽  
pp. 205-219 ◽  
Author(s):  
William T. Mason ◽  
Dennis W. Waring
Keyword(s):  
Patch Clamp ◽  
Ion Channel ◽  
Channel Activation ◽  
Releasing Hormone ◽  
Gonadotrophin Releasing Hormone

scholarly journals Functional Expression of TRPV1 Ion Channel in the Canine Peripheral Blood Mononuclear Cells

2021 ◽  
Vol 22 (6) ◽  
pp. 3177
Author(s):  
Joanna K. Bujak ◽  
Daria Kosmala ◽  
Kinga Majchrzak-Kuligowska ◽  
Piotr Bednarczyk
Keyword(s):  
Patch Clamp ◽  
Ion Channel ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Functional Expression ◽  
Peripheral Blood Mononuclear ◽  
Whole Cell ◽  
Whole Cell Patch Clamp ◽  
Blood Mononuclear Cells

TRPV1, known as a capsaicin receptor, is the best-described transient receptor potential (TRP) ion channel. Recently, it was shown to be expressed by non-excitable cells such as lymphocytes. However, the data regarding the functional expression of the TRPV1 channel in the immune cells are often contradictory. In the present study, we performed a phylogenetical analysis of the canine TRP ion channels, we assessed the expression of TRPV1 in the canine peripheral blood mononuclear cells (PBMC) by qPCR and Western blot, and we determined the functionality of TRPV1 by whole-cell patch-clamp recordings and calcium assay. We found high expression of TRPV2, -M2, and -M7 in the canine PBMCs, while expression of TRPV1, -V4 and, -M5 was relatively low. We confirmed that TRPV1 is expressed on the protein level in the PBMC and it localizes in the plasma membrane. The whole-cell patch-clamp recording revealed that capsaicin application caused a significant increase in the current density. Similarly, the results from the calcium assay show a dose-dependent increase in intracellular calcium level in the presence of capsaicin that was partially abolished by capsazepine. Our study confirms the expression of TRPV1 ion channel on both mRNA and protein levels in the canine PBMC and indicates that the ion channel is functional.

The influence of TRPM8 ion channel activation on immune response at different temperature conditions

2012 ◽  
Vol 37 (8) ◽  
pp. 648-653 ◽  
Author(s):  
Tamara V. Kozyreva ◽  
Galina M. Khramova ◽  
Ludmila S. Eliseeva
Keyword(s):  
Immune Response ◽  
Ion Channel ◽  
Channel Activation ◽  
Temperature Conditions
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