Dynamic Ion Channel Activation Scheduling in Patch Clamp on a Chip

2004 ◽  
Vol 3 (3) ◽  
pp. 217-224 ◽  
Author(s):  
V. Krishnamurthy
Keyword(s):  
Patch Clamp ◽  
Ion Channel ◽  
Channel Activation

scholarly journals Functional Expression of TRPV1 Ion Channel in the Canine Peripheral Blood Mononuclear Cells

2021 ◽  
Vol 22 (6) ◽  
pp. 3177
Author(s):  
Joanna K. Bujak ◽  
Daria Kosmala ◽  
Kinga Majchrzak-Kuligowska ◽  
Piotr Bednarczyk
Keyword(s):  
Patch Clamp ◽  
Ion Channel ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Functional Expression ◽  
Peripheral Blood Mononuclear ◽  
Whole Cell ◽  
Whole Cell Patch Clamp ◽  
Blood Mononuclear Cells

TRPV1, known as a capsaicin receptor, is the best-described transient receptor potential (TRP) ion channel. Recently, it was shown to be expressed by non-excitable cells such as lymphocytes. However, the data regarding the functional expression of the TRPV1 channel in the immune cells are often contradictory. In the present study, we performed a phylogenetical analysis of the canine TRP ion channels, we assessed the expression of TRPV1 in the canine peripheral blood mononuclear cells (PBMC) by qPCR and Western blot, and we determined the functionality of TRPV1 by whole-cell patch-clamp recordings and calcium assay. We found high expression of TRPV2, -M2, and -M7 in the canine PBMCs, while expression of TRPV1, -V4 and, -M5 was relatively low. We confirmed that TRPV1 is expressed on the protein level in the PBMC and it localizes in the plasma membrane. The whole-cell patch-clamp recording revealed that capsaicin application caused a significant increase in the current density. Similarly, the results from the calcium assay show a dose-dependent increase in intracellular calcium level in the presence of capsaicin that was partially abolished by capsazepine. Our study confirms the expression of TRPV1 ion channel on both mRNA and protein levels in the canine PBMC and indicates that the ion channel is functional.

The influence of TRPM8 ion channel activation on immune response at different temperature conditions

2012 ◽  
Vol 37 (8) ◽  
pp. 648-653 ◽  
Author(s):  
Tamara V. Kozyreva ◽  
Galina M. Khramova ◽  
Ludmila S. Eliseeva
Keyword(s):  
Immune Response ◽  
Ion Channel ◽  
Channel Activation ◽  
Temperature Conditions

Ion channel activities of cultured rat mesangial cells

1991 ◽  
Vol 261 (5) ◽  
pp. F808-F814 ◽  
Author(s):  
H. Matsunaga ◽  
N. Yamashita ◽  
Y. Miyajima ◽  
T. Okuda ◽  
H. Chang ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Ion Channels ◽  
Patch Clamp ◽  
Ion Channel ◽  
Arginine Vasopressin ◽  
Mesangial Cells ◽  
Cation Channel ◽  
K Channel ◽  
Patch Clamp Technique ◽  
Inside Out

We used the patch-clamp technique to clarify the nature of ion channels in renal mesangial cells in culture. In the cell-attached mode most patches were silent in the absence of agonists. In some patches a 25-pS nonselective channel was observed. This 25-pS cation channel was consistently observed in inside-out patches, and it was activated by intracellular Ca2+. Excised patch experiments also revealed the existence of a 40-pS K+ channel, which was activated by intracellular Ca2+. This 40-pS K+ channel was observed infrequently in the cell-attached mode. The activities of both channels were increased by arginine vasopressin or angiotensin II, resulting from an increase in intracellular Ca2+ concentration.

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