Precise Ultrastructural Localization of Endothelial Leukocyte Adhesion Molecule-1 Vascular Cell Adhesion Molecule-1 and Intercellular Adhesion Molecule-1 in Patients with IgA Nephropathy

Nephron ◽  
1997 ◽  
Vol 75 (1) ◽  
pp. 54-64 ◽  
Author(s):  
Takahiko Ogawa ◽  
Noriaki Yorioka ◽  
Takafumi Ito ◽  
Satoshi Ogata ◽  
Junko Kumagai ◽  
...  
Keyword(s):  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Leukocyte Adhesion ◽  
Ultrastructural Localization ◽  
Intercellular Adhesion Molecule ◽  
Vascular Cell Adhesion ◽  
Intercellular Adhesion Molecule 1 ◽  
Leukocyte Adhesion Molecule ◽  
Endothelial Leukocyte Adhesion Molecule ◽  
Cell Adhesion Molecule 1

Human monocytes bind to two cytokine-induced adhesive ligands on cultured human endothelial cells: endothelial-leukocyte adhesion molecule-1 and vascular cell adhesion molecule-1

Blood ◽  
1991 ◽  
Vol 77 (10) ◽  
pp. 2266-2271 ◽  
Author(s):  
T Carlos ◽  
N Kovach ◽  
B Schwartz ◽  
M Rosa ◽  
B Newman ◽  
...  
Keyword(s):  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Leukocyte Adhesion ◽  
Vascular Cell Adhesion ◽  
Vascular Cell ◽  
Leukocyte Adhesion Molecule ◽  
Endothelial Leukocyte Adhesion Molecule ◽  
Adhesive Proteins ◽  
Cell Adhesion Molecule 1 ◽  
Monocyte Adherence

Abstract Vascular cell adhesion molecule-1 (VCAM-1) and endothelial-leukocyte adhesion molecule-1 (ELAM-1) are adhesive proteins induced on endothelium by cytokines. We examined the contribution of these adhesive proteins to human peripheral blood monocyte adherence to endothelium using transfected Chinese hamster ovary (CHO) cells stably expressing these proteins and monoclonal antibodies (MoAbs) to ELAM-1, VCAM-1, or CD49d/CD29 (VLA-4), the leukocyte receptor for VCAM-1. Monocytes bound to CHO cells transfected with cDNA of ELAM-1 or VCAM-1. Binding to ELAM-1 was inhibited by MoAb to ELAM-1 and binding to VCAM-1 was inhibited by MoAb to VCAM-1 or the alpha-chain of very late activation antigen-4 (VLA-4) (CD49d). Additive inhibition of adherence to unstimulated human umbilical vein endothelium (HUVE) was observed when monocytes were pretreated with both MoAb to CD49d and MoAb to CD18, the common beta-chain of the leukocyte beta 2 integrin receptors. Adherence of monocytes to HUVE stimulated by recombinant human tumor necrosis factor-alpha was not reduced by MoAbs to CD18, CD49d, or ELAM- 1 when used singly, but combinations of these MoAbs produced significant inhibition. We conclude that multiple receptor-ligand systems are involved in monocyte adherence to endothelium.

scholarly journals Monocyte attachment to activated human vascular endothelium in vitro is mediated by leukocyte adhesion molecule-1 (L-selectin) under nonstatic conditions.

1992 ◽  
Vol 175 (6) ◽  
pp. 1789-1792 ◽  
Author(s):  
O Spertini ◽  
F W Luscinskas ◽  
M A Gimbrone ◽  
T F Tedder
Keyword(s):  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Cell Attachment ◽  
Leukocyte Adhesion ◽  
Vascular Cell Adhesion ◽  
Leukocyte Adhesion Molecule ◽  
Endothelial Monolayers ◽  
Endothelial Leukocyte Adhesion Molecule ◽  
Cell Adhesion Molecule 1

The receptors that mediate monocyte adhesion to cytokine-stimulated endothelial monolayers were assessed using a nonstatic (rotating) cell-attachment assay. In this system, leukocyte adhesion molecule-1 (LAM-1) (L-selectin) mediated a major portion (87 +/- 15% at 37 degrees C) of monocyte attachment to activated endothelium. mAb blocking of endothelial leukocyte adhesion molecule-1 (41% inhibition), CD18 (36%), and vascular cell adhesion molecule-1 (25%) function had lesser effects on attachment. These results suggest that LAM-1 may serve an important role in monocyte attachment to endothelium at sites of inflammation.

scholarly journals Human monocytes bind to two cytokine-induced adhesive ligands on cultured human endothelial cells: endothelial-leukocyte adhesion molecule-1 and vascular cell adhesion molecule-1

Blood ◽  
1991 ◽  
Vol 77 (10) ◽  
pp. 2266-2271
Author(s):  
T Carlos ◽  
N Kovach ◽  
B Schwartz ◽  
M Rosa ◽  
B Newman ◽  
...  
Keyword(s):  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Leukocyte Adhesion ◽  
Vascular Cell Adhesion ◽  
Vascular Cell ◽  
Leukocyte Adhesion Molecule ◽  
Endothelial Leukocyte Adhesion Molecule ◽  
Adhesive Proteins ◽  
Cell Adhesion Molecule 1 ◽  
Monocyte Adherence

Vascular cell adhesion molecule-1 (VCAM-1) and endothelial-leukocyte adhesion molecule-1 (ELAM-1) are adhesive proteins induced on endothelium by cytokines. We examined the contribution of these adhesive proteins to human peripheral blood monocyte adherence to endothelium using transfected Chinese hamster ovary (CHO) cells stably expressing these proteins and monoclonal antibodies (MoAbs) to ELAM-1, VCAM-1, or CD49d/CD29 (VLA-4), the leukocyte receptor for VCAM-1. Monocytes bound to CHO cells transfected with cDNA of ELAM-1 or VCAM-1. Binding to ELAM-1 was inhibited by MoAb to ELAM-1 and binding to VCAM-1 was inhibited by MoAb to VCAM-1 or the alpha-chain of very late activation antigen-4 (VLA-4) (CD49d). Additive inhibition of adherence to unstimulated human umbilical vein endothelium (HUVE) was observed when monocytes were pretreated with both MoAb to CD49d and MoAb to CD18, the common beta-chain of the leukocyte beta 2 integrin receptors. Adherence of monocytes to HUVE stimulated by recombinant human tumor necrosis factor-alpha was not reduced by MoAbs to CD18, CD49d, or ELAM- 1 when used singly, but combinations of these MoAbs produced significant inhibition. We conclude that multiple receptor-ligand systems are involved in monocyte adherence to endothelium.

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