Prognostic Value of Bone Marrow Acid Phosphatase in Prostatic Carcinoma

During the design and synthesis of new chemotherapeutic agents for prostatic carcinoma based on phosphorylated agents which might be enzyme-activated to cytotoxicity, phosphorylcholine, [(CH3)3+NCH2CH2OPO3Ca]Cl-, has been indicated to be a very specific substrate for prostatic acid phosphatase (PAP). This phenomenon has led to the development of specific histochemical and ultracytochemical methods for PAP using modifications of the Gomori lead method for acid phosphatase. Comparative histochemical results in prostate and kidney of the rat have been published earlier with phosphorylcholine (PC) and β-glycerophosphate (βGP). We now report the ultracytochemical results.Minced tissues were fixed in 3% glutaraldehyde-0.1 M phosphate buffered (pH 7.4) for 1.5 hr and rinsed overnight in several changes of 0.05 M phosphate buffer (pH 7.0) containing 7.5% sucrose. Tissues were incubated 30 min to 2 hr in Gomori acid phosphatase medium (2) containing 0.1 M substrate, either PC or βGP.

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Disseminated tumor cells in the bone marrow of patients with operable primary breast cancer: prognostic value and tumor phenotype

Geburtshilfe und Frauenheilkunde ◽

10.1055/s-0034-1388345 ◽

2014 ◽

Vol 74 (S 01) ◽

Author(s):

S Stefanovic ◽

I Diel ◽

P Sinn ◽

C Sohn ◽

F Schuetz ◽

...

Keyword(s):

Breast Cancer ◽

Bone Marrow ◽

Tumor Cells ◽

Primary Breast Cancer ◽

Prognostic Value ◽

Disseminated Tumor Cells ◽

Tumor Phenotype

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Congenital neutropenia: neutrophil proliferation with abnormal maturation

Blood ◽

10.1182/blood.v46.5.723.723 ◽

1975 ◽

Vol 46 (5) ◽

pp. 723-734 ◽

Cited By ~ 41

Author(s):

RT Parmley ◽

M Ogawa ◽

CP Jr Darby ◽

SS Spicer

Keyword(s):

Bone Marrow ◽

Acid Phosphatase ◽

Colony Stimulating Factor ◽

Peripheral Blood Leukocytes ◽

Congenital Neutropenia ◽

Blood Leukocytes ◽

Specific Granules ◽

Primary Granules ◽

Stimulating Factor ◽

Myelin Figures

Abstract A child with congenital neutropenia was studied using bone marrow culture and ultrastructural and cytochemical techniques. The patient's marrow cells formed a large number of granulocytic colonies of normal size in culture, and her peripheral blood leukocytes produced adequate colony-stimulating factor. No serum inhibitors were identified. The patient's promyelocytes from direct marrow and culture appeared normal in ultrastructure, and primary granules, contained peroxidase and acid phosphatase activity. Myelocytes and rare segmented neutrophils from direct marrow specimens demonstrated atypical notched nuclei, myelin figures in Golgi lamellae and primary (azurophilic) granules, and no identifiable secondary (specific) granules. These data indicate an intrinsic neutrophil defect which allows normal proliferation of precursor cells, but results in abnormal granulogenesis and apparent inability to form secondary granules.

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PHOSPHODIESTERASE AND ACID PHOSPHATASE ACTIVITIES OF AVIAN BONE MARROW CELLS

Canadian Journal of Biochemistry ◽

10.1139/o65-146 ◽

1965 ◽

Vol 43 (8) ◽

pp. 1319-1328 ◽

Cited By ~ 2

Author(s):

Gerald Kingsley Bristow ◽

Esther W. Yamada

Keyword(s):

Bone Marrow ◽

Acid Phosphatase ◽

Bone Marrow Cells ◽

Intracellular Distribution ◽

Specific Substrate ◽

Magnesium Ions ◽

Differential Centrifugation ◽

Alkaline Ph ◽

Ph Optimum ◽

Rna And Dna

Avian bone marrow has been found to contain a phosphodiesterase as well as an acid phosphatase. Some properties of these enzymes have been described. Because the phosphodiesterase of this tissue has an alkaline pH optimum, is activated by magnesium ions, and acts on the specific substrate p-nitrophenyl thymidine 5′-phosphate, it is probably a phosphodiesterase I such as is present in snake venom and other tissues.The intracellular distribution of these two enzymes in normal and regenerating bone marrow was studied. Subcellular fractions were prepared by differential centrifugation or by centrifugation through sucrose density gradients. The RNA and DNA content of each fraction was determined. By the methods used no differences in the properties or intracellular distribution of the two enzymes in normal and regenerating bone marrow were found.

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The Role of the Radioimmunoassay for Prostatic Acid Phosphatase in Prostatic Carcinoma

Urologic Clinics of North America ◽

10.1016/s0094-0143(21)00153-1 ◽

1980 ◽

Vol 7 (3) ◽

pp. 645-652 ◽

Cited By ~ 1

Author(s):

Andrew W. Bruce ◽

Donald E. Mahan ◽

William D. Belville

Keyword(s):

Acid Phosphatase ◽

Prostatic Carcinoma ◽

Prostatic Acid Phosphatase

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