Dopamine in Rat Kidney Slices � Its Production and Its Histofluorescent Localization

2015 ◽  
pp. 140-144
Author(s):  
Jacqueline Hagege ◽  
Fayez Wahbe ◽  
Andreas Wiemeyer ◽  
Gabriel Richet
Keyword(s):  
Rat Kidney ◽  
Kidney Slices

Studies of calcium-45 desaturation from kidney slices in flow-through chambers

1978 ◽  
Vol 234 (1) ◽  
pp. R34-R38
Author(s):  
T. Uchikawa ◽  
A. B. Borle
Keyword(s):  
Kinetic Parameters ◽  
Rat Kidney ◽  
Kidney Cells ◽  
Tissue Slices ◽  
Kidney Slices ◽  
System A ◽  
Order Of Magnitude ◽  
Exponential Equations ◽  
Flow Through ◽  
Best Fit

This paper describes a method to measure calcium fluxes and calcium exchangeable pools in tissue slices by continuous perifusion in flow-through chambers. 45Ca desaturation from rat kidney slices can be analyzed as in an open three-compartment catenary system. A set of equations is given to calculate all the relevant kinetic parameters from the triple exponential equations which best fit the desaturation curves. The results show that the kinetic parameters obtained in kidney slices by this new method are in the same order of magnitude as those previously observed in cultured monkey kidney cells.

Inhibition of renin release from rat kidney slices by the angiotensins

1978 ◽  
Vol 235 (1) ◽  
pp. F62-F68 ◽  
Author(s):  
A. J. Naftilan ◽  
S. Oparil
Keyword(s):  
Direct Action ◽  
Rat Kidney ◽  
Sharp Decrease ◽  
Renin Release ◽  
Structural Basis ◽  
Response Curves ◽  
Amino Terminal ◽  
Kidney Slices ◽  
Angiotensin Iii ◽  
Terminal Amino

The mechanism and structural basis of the inhibition of renin release by angiotensin II (AII) were studied in rat kidney slices. Renin release was inhibited by AII and the (2–8), (3–8), (4–8), and (5–8) peptides of AII (5 X 10(-5) M). These constituent peptides of AII which share a common carboxyl terminus inhibited renin release with a sharp decrease in potency when the amino-terminal amino acid was removed. Saralasin attenuated the inhibition of renin release induced by equimolar concentrations of AII. Dose-response curves for AII and the (2–8) peptide [angiotensin III (AIII)] indicate that AII is a more potent inhibitor of renin release than is AIII. Depletion of renal norepinephrine by reserpine (10 mg/kg, i.p.) or pretreatment of slices with papaverine (1 X 10(-4) M) did not block the action of AII. The data give evidence for a direct action of AII on the juxtaglomerular cells independent of an interaction with either the sympathetic nervous system or the arteriolar baroreceptor and suggest that the intrarenal receptors that mediate AII-induced inhibition of renin release differ from AII receptors in the adrenal cortex.

Beta-1 adrenergic inhibition of kallikrein release from rat kidney cortical slices

1990 ◽  
Vol 258 (5) ◽  
pp. F1425-F1431
Author(s):  
J. P. Girolami ◽  
J. L. Bascands ◽  
P. Valet ◽  
C. Pecher ◽  
G. Cabos
Keyword(s):  
De Novo ◽  
Rat Kidney ◽  
Inhibitory Effect ◽  
Significant Inhibition ◽  
Sodium Diet ◽  
Kidney Slices ◽  
Low Sodium Diet ◽  
Rat Cortical Slices ◽  
Cortical Slices

Renal storage; release, and biosynthesis of kallikrein were studied using rat cortical slices. This model permitted the study of the direct effect of norepinephrine on the renal kallikrein system in the absence of changes in perfusion pressure. Kallikrein was measured by its kininogenase activity and its direct immunoreactive concentration. Under basal conditions, rat kidney cortical slices synthesize and release glandular kallikrein in vitro at a linear rate for up to 40 min. Kidney slices obtained from rats fed with a low-sodium diet (LS) released more kallikrein into the incubation medium than slices from rats under a normal-sodium diet (NS). Cycloheximide and incubation at 4 degrees C inhibited the release and the biosynthesis of kallikrein independently of the sodium diet. Addition of norepinephrine (NE, 10(-8)-10(-5) M) induced a similar dose-dependent inhibition of kallikrein secretion, which reached -27 +/- 8% in NS rats and -29 +/- 9% in LS rats with 10(-7) M NE. This inhibition of the secretion was associated with an increase in tissue kallikrein concentration in kidney slices from rats on both sodium diets. However, a significant inhibition of the calculated net de novo synthesis was only observed in LS rats. In both groups of animals the ratio of active to total kallikrein was unchanged. The inhibitory effect of kallikrein secretion by NE was never modified in the presence of the alpha-antagonist phentolamine (10(-6) M). In contrast the beta-antagonist propranolol (10(-6) M) prevented the inhibitory effect of 10(-7) M NE.(ABSTRACT TRUNCATED AT 250 WORDS)

Effect of D2O in vivo and in vitro on uptake of p-aminohippurate by rat kidney slices

1959 ◽  
Vol 197 (5) ◽  
pp. 1128-1130 ◽  
Author(s):  
John F. Thomson ◽  
Florence J. Klipfel
Keyword(s):  
Oxygen Consumption ◽  
Rat Kidney ◽  
Body Water ◽  
The Body ◽  
Kidney Slices

Kidney slices from rats in which one-third of the body water was replaced by D2O showed no impairment of the capacity to accumulate PAH, despite the moribund condition of the animals. In vitro, 100% D2O inhibited PAH uptake by 65%, oxygen consumption by only 15%, during a 1-hour incubation of kidney slices; very little PAH was taken up after the first 30 minutes.

Freezing point depression of rat kidney slices during water diuresis and antidiuresis

1960 ◽  
Vol 199 (5) ◽  
pp. 915-918 ◽  
Author(s):  
George A. Bray
Keyword(s):  
Pressure Gradient ◽  
Osmotic Pressure ◽  
Freezing Point ◽  
Rat Kidney ◽  
Freezing Point Depression ◽  
Water Diuresis ◽  
Outer Medulla ◽  
Kidney Slices ◽  
Outer Stripe ◽  
Osmotic Pressure Gradient

The freezing point depression of slices of rat kidney removed during water diuresis or antidiuresis has been investigated with a microcryoscopic method. The osmotic pressure gradient in the inner medulla first demonstrated by Wirz has been confirmed. The inner medulla was found to be hypertonic to plasma during water diuresis. Hypotonic tubules were present throughout the cortex and outer stripe of the outer medulla.

scholarly journals HISTOCHEMICAL STUDIES ON THE UPTAKE OF HORSERADISH PEROXIDASE BY RAT KIDNEY SLICES

1965 ◽  
Vol 27 (2) ◽  
pp. 305-312 ◽  
Author(s):  
A. T. Miller ◽  
D. M. Hale ◽  
K. D. Alexander
Keyword(s):  
Horseradish Peroxidase ◽  
Intracellular Transport ◽  
Rat Kidney ◽  
Kidney Slices ◽  
Metabolic Conditions ◽  
Peroxidase Uptake ◽  
Free Media ◽  
Energy Dependent ◽  
Convoluted Tubules ◽  
Proximal Convoluted Tubules

When rat kidney slices were incubated in the presence of horseradish peroxidase, there was an energy-dependent uptake of the protein by the cells of the kidney tubules. The uptake was greatest in the proximal convoluted tubules and in the thick ascending limbs of the loops of Henle; it was abolished by cold, anoxia, 2,4-dinitrophenol, and fluoroacetate, and was more readily depressed by unfavorable metabolic conditions in the proximal convoluted tubules than in the thick ascending limbs. Protein uptake was inhibited when the kidney slices were incubated in electrolyte-free media. In sodium chloride solutions, uptake was reduced as sodium was progressively replaced by choline, and ouabain inhibited uptake in the proximal convoluted tubules, but not in the thick ascending limbs. To a limited extent, lithium could replace sodium in the incubation medium with no depression of peroxidase uptake. These results suggest that a sodium-stimulated, ouabain-sensitive ATPase may be involved in the uptake of protein by cells of the kidney tubule. The intracellular transport of peroxidase in cells of the proximal convoluted tubules was abolished by cold, anoxia, and 2,4-dinitrophenol, but it was not affected by concentrations of ouabain which inhibited the uptake of the protein.

Organic mercurials and net movements of potassium in rat kidney slices

1967 ◽  
Vol 213 (5) ◽  
pp. 1209-1217 ◽  
Author(s):  
FJ Bowman ◽  
EJ Landon
Keyword(s):  
Rat Kidney ◽  
Kidney Slices ◽  
Organic Mercurials

Ammonia production and pathways of glutamine utilization in rat kidney slices

1976 ◽  
Vol 444 (3) ◽  
pp. 644-652 ◽  
Author(s):  
T.C. Welbourne ◽  
D. Francoeur ◽  
G. Thornley-Brown ◽  
C.J. Welbourne
Keyword(s):  
Rat Kidney ◽  
Ammonia Production ◽  
Kidney Slices
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