Electron Microscopy of Liver Biopsies in the Diagnosis of Pediatric Hepatic Disorders1

2015 ◽  
pp. 246-267 ◽  
Author(s):  
Theodore C. Iancu
Keyword(s):  
Electron Microscopy ◽  
Liver Biopsies

Contribution of electron microscopy to the pathological diagnosis in routine liver biopsies

1989 ◽  
Vol 20 (1) ◽  
pp. 45-50
Author(s):  
C. Jardon-Jeghers ◽  
M. Vivario ◽  
J.M. Rakic ◽  
N. Remacle ◽  
J. Boniver
Keyword(s):  
Electron Microscopy ◽  
Pathological Diagnosis ◽  
Liver Biopsies

Tissue and Hepatic Subcellular Distribution of Liposomes Containing Bleomycin after Intravenous Administration to Patients with Neoplasms

1976 ◽  
Vol 51 (4) ◽  
pp. 421-425 ◽  
Author(s):  
A. W. Segal ◽  
G. Gregoriadis ◽  
J. P. Lavender ◽  
D. Tarin ◽  
T. J. Peters
Keyword(s):  
Electron Microscopy ◽  
Tissue Distribution ◽  
Subcellular Distribution ◽  
The Other ◽  
Whole Body ◽  
Post Mortem ◽  
Malignant Tissue ◽  
Body Scanning ◽  
Liver Biopsies

1. Liposomes containing 111In-labelled bleomycin were injected intravenously into two patients. One patient had a hepatoma and the other had secondary adenocarcinomatous deposits in the liver. 2. The tissue distribution of 111In was determined by whole-body scanning and by measurement of the radioactivity in organs at autopsy. 3. Scans in vivo and post-mortem measurement of radioactivity indicated that liposomes accumulate predominantly in the liver, but that there is no selective uptake of liposomes by the malignant tissue. 4. The subcellular distribution of radioactivity in the liver was measured 90 min after injection by fractionation of percutaneous liver biopsies on sucrose density gradients. 5. Radioactivity within the liver was concentrated in lysosomes. 6. Electron microscopy of tissue obtained before the administration of liposomes revealed particles morphologically indistinguishable from liposomes in hepatoma cells and hepatocytes.

Hepatic Ultrastructure in Methotrexate Treated Psoriatic Patients

1977 ◽  
Vol 35 ◽  
pp. 520-521
Author(s):  
E. Horvath ◽  
K. Kovacs ◽  
R. C. Ross ◽  
N. A. Kerenyi ◽  
F. G. Saibil
Keyword(s):  
Electron Microscopy ◽  
Folic Acid ◽  
Cell Membrane ◽  
Liver Tissue ◽  
Percutaneous Biopsy ◽  
Giant Mitochondria ◽  
Folic Acid Antagonist ◽  
Zone Cell ◽  
Paracrystalline Inclusions ◽  
Liver Biopsies

Fifty liver biopsies of psoriatic patients treated with methotrexate, a folic acid antagonist, for a period from a few weeks up to 15 years have been studied. Liver tissue has been obtained by percutaneous biopsy with a Menghini needle and prepared for electron microscopy by using conventional techniques.In the hepatocytes, profound changes have been revealed in four areas: nucleus, mitochondria, ectoplasmic zone-cell membrane and desmosomes. The nuclei showed reduced electron opacity and almost complete absence of heterochromatin. Cytoplasmic invaginations and glycogen inclusions were common. Mitochondrial pleomorphism, paracrystalline inclusions and giant mitochondria were prominent findings. Compartmentalization, fission and division of mitochondria were also noted. Sequestration of mitochondria via development of cytosegresomes was observed in several cases. Detachment of desmosomes was a conspicuous finding in 25 of 50 biopsies obtained from methotrexate-treated psoriatics. In these livers many or practically all membrane sites with desmosomal specialization appeared to be pulled apart.

Fixation artifacts in percutaneous needle biopsies of liver

1993 ◽  
Vol 51 ◽  
pp. 20-21
Author(s):  
T.C. Januszewski ◽  
J.M. Harb ◽  
R.A. Komorowski
Keyword(s):  
Electron Microscopy ◽  
Standard Procedure ◽  
Light And Electron Microscopy ◽  
Significant Loss ◽  
Electron Microscopic ◽  
Initial Fixation ◽  
Cacodylate Buffer ◽  
Microscopic Studies ◽  
Block Face ◽  
Liver Biopsies

Percutaneous needle biopsy is a standard procedure for obtaining liver tissues for pathologic studies by light and electron microscopy. Tissues obtained by this procedure usually measure 1mm to 1.5 mm in diameter, and can be placed immediately at the bedside into a standard glutaraldehyde fixative for processing by the electron microscopy laboratory. Although the biopsy thickness is within the penetration range of glutaraldehyde, we have noted a significant loss of preservation in the center of most liver biopsies. The loss is apparent at light and electron microscopic levels. The purpose of this paper is to illustrate the artifact and to discuss the importance of avoiding the artifact when selecting areas of the specimen for electron microscopic studies.Secimens studied were routine liver biopsies submitted to the electron microscopy laboratory after an initial fixation of one hour in 2.5% glutaraldehyde in 0.1M cacodylate buffer. To illustrate the artifact, one-micron thick sections of the entire block face were stained with toluidine blue for light microscopic study (Figure 1).

scholarly journals Electron Microscopy of Liver Biopsies

Liver Biopsy ◽  
10.5772/20385 ◽  
2011 ◽  
Author(s):  
Theodore C. ◽  
Irena Manov
Keyword(s):  
Electron Microscopy ◽  
Liver Biopsies

Platinum Compounds as Stains for Electron Microscopy

1974 ◽  
Vol 32 ◽  
pp. 230-231
Author(s):  
S. K. Aggarwal ◽  
P. McAllister ◽  
R. W. Wagner ◽  
B. Rosenberg
Keyword(s):  
Electron Microscopy ◽  
Hela Cells ◽  
Uranyl Acetate ◽  
Sarcoma 180 ◽  
Platinum Compounds ◽  
Kb Cells ◽  
En Bloc ◽  
Human Lymphoma ◽  
Ultrathin Sections ◽  
Blue Coloration

Uranyl acetate has been used as an electron stain for en bloc staining as well as for staining ultrathin sections in conjunction with various lead stains (Fig. 1). Present studies reveal that various platinum compounds also show promise as electron stains. Certain platinum compounds have been shown to be effective anti-tumor agents. Of particular interest are the compounds with either uracil or thymine as one of the ligands (cis-Pt(II)-uracil; cis-Pt(II)-thymine). These compounds are amorphous, highly soluble in water and often exhibit an intense blue coloration. These compounds show enough electron density to be used as stains for electron microscopy. Most of the studies are based on various cell lines (human AV, cells, human lymphoma cells, KB cells, Sarcoma-180 ascites cells, chick fibroblasts and HeLa cells) while studies on tissue blocks are in progress.

Crystalloid Inclusions in Hepatocyte Mitochondria and Their Similarity to Cytoplasmic Crystalloids

1974 ◽  
Vol 32 ◽  
pp. 198-199
Author(s):  
Odell T. Minick ◽  
Hidejiro Yokoo
Keyword(s):  
Liver Disease ◽  
Alcoholic Liver Disease ◽  
Special Interest ◽  
Structural Variations ◽  
Mitochondrial Alterations ◽  
The Matrix ◽  
Crystalloid Inclusions ◽  
Liver Biopsies

Mitochondrial alterations were studied in 25 liver biopsies from patients with alcoholic liver disease. Of special interest were the morphologic resemblance of certain fine structural variations in mitochondria and crystalloid inclusions. Four types of alterations within mitochondria were found that seemed to relate to cytoplasmic crystalloids.Type 1 alteration consisted of localized groups of cristae, usually oriented in the long direction of the organelle (Fig. 1A). In this plane they appeared serrated at the periphery with blind endings in the matrix. Other sections revealed a system of equally-spaced diagonal lines lengthwise in the mitochondrion with cristae protruding from both ends (Fig. 1B). Profiles of this inclusion were not unlike tangential cuts of a crystalloid structure frequently seen in enlarged mitochondria described below.

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