Detection of Wheat Lipid Transfer Protein (Tri a 14) Sensitization: Comparison between Currently Available Diagnostic Tools

2021 ◽  
pp. 1-5
Author(s):  
Clara San Bartolomé ◽  
Rosa Muñoz-Cano ◽  
Josefina Rius ◽  
Rocío Casas-Saucedo ◽  
Sara Balsells ◽  
...  
Keyword(s):  
Lipid Transfer Protein ◽  
Poor Performance ◽  
Specific Ige ◽  
Diagnostic Tools ◽  
Lipid Transfer ◽  
Prick Test ◽  
Transfer Protein ◽  
Whole Wheat ◽  
Systemic Reactions ◽  
Routine Diagnosis

<b><i>Background:</i></b> Wheat lipid transfer protein (LTP; Tri a 14) and ω5-gliadin have been described as major allergens in wheat allergy (WA) and relevant in wheat-induced anaphylaxis, frequently associated with cofactors. <b><i>Objective:</i></b> The objective of this study was to compare tools currently available in routine diagnosis to detect Tri a 14 sensitization, its clinical relevance, and cosensitization to ω5-gliadin and other LTPs. <b><i>Methods:</i></b> One hundred eighteen adults sensitized to rTri a 14 by ImmunoCAP<sup>®</sup> (cutoff ≥0.1 kU<sub>A</sub>/L) identified among 210 LTP allergic patients were included. We evaluated (1) wheat skin prick test (SPT), (2) specific IgE (sIgE) to wheat, rTri a 14, rTri a 19, peach, apple, walnut, hazelnut, and peanut LTPs using ImmunoCAP<sup>®</sup> and microarray ImmunoCAP®ISAC (cutoff ≥0.3I SU), and (3) wheat-related symptoms. <b><i>Results:</i></b> Wheat SPT and sIgE were positive in 31% and 85% of subjects, respectively. rTri a 14 by microarray was detected in 25%. Eight percent showed cosensitization to ω5-gliadin. Thirty percent referred symptoms (gastrointestinal [13%], urticaria [11%], and anaphylaxis [8%]). Cofactors (45%) were significantly associated with systemic reactions. <b><i>Conclusion:</i></b> WA due to Tri a 14 is frequently related with systemic reactions and because are frequently related to cofactors, the culprit may not be suspected. Together with the poor performance to identify Tri a 14 sensitization of the current routine diagnostic tools based on the analysis of whole wheat extract, such as wheat SPT or sIgE, there is a high risk that WA may be overlooked. Thus, when WA is suspected, sIgE Tri a 14 assessment is recommended, together with wheat and ω5-gliadin, preferably in the singleplex format, and carefully evaluated considering ≥0.1 kUA/L as a cutoff.

Performance of basophil activation test and specific IgG4 as diagnostic tools in nonspecific lipid transfer protein allergy: Antwerp‐Barcelona comparison

Allergy ◽  
2019 ◽  
Vol 75 (3) ◽  
pp. 616-624 ◽  
Author(s):  
Ine I. Decuyper ◽  
Mariona Pascal ◽  
Athina L. Van Gasse ◽  
Christel Mertens ◽  
Araceli Díaz‐Perales ◽  
...  
Keyword(s):  
Lipid Transfer Protein ◽  
Basophil Activation Test ◽  
Basophil Activation ◽  
Diagnostic Tools ◽  
Lipid Transfer ◽  
Activation Test ◽  
Transfer Protein ◽  
Specific Igg4 ◽  
Nonspecific Lipid Transfer Protein

scholarly journals Structural and Functional Characterization of Recombinant Isoforms of the Lentil Lipid Transfer Protein

Acta Naturae ◽  
2015 ◽  
Vol 7 (3) ◽  
pp. 65-73 ◽  
Author(s):  
I. V. Bogdanov ◽  
E. I. Finkina ◽  
S. V. Balandin ◽  
D. N. Melnikova ◽  
E. A. Stukacheva ◽  
...  
Keyword(s):  
Disulfide Bonds ◽  
Lipid Transfer Protein ◽  
Functional Characterization ◽  
Helical Structure ◽  
Specific Ige ◽  
Food Allergies ◽  
Lipid Transfer ◽  
E Coli ◽  
Transfer Protein

The recombinant isoforms Lc-LTP1 and Lc-LTP3 of the lentil lipid transfer protein were overexpressed in E. coli cells. It was confirmed that both proteins are stabilized by four disulfide bonds and characterized by a high proportion of the -helical structure. It was found that Lc-LTP1 and Lc-LTP3 possess antimicrobial activity and can bind fatty acids. Both isoforms have the ability to bind specific IgE from sera of patients with food allergies, which recognize similar epitopes of the major peach allergen Pru p 3. Both isoforms were shown to have immunological properties similar to those of other plant allergenic LTPs, but Lc-LTP3 displayed a less pronounced immunoreactivity.

scholarly journals Nickel allergy in lipid transfer protein sensitized patients: Prevalence and clinical features

2020 ◽  
Vol 34 ◽  
pp. 205873842097489
Author(s):  
Angela Rizzi ◽  
Raffaella Chini ◽  
Riccardo Inchingolo ◽  
Valentina Carusi ◽  
Franco Pandolfi ◽  
...  
Keyword(s):  
Patch Test ◽  
Clinical Features ◽  
Lipid Transfer Protein ◽  
Nickel Sulfate ◽  
Specific Ige ◽  
Lipid Transfer ◽  
Nickel Allergy ◽  
Transfer Protein ◽  
Mediterranean Countries ◽  
Allergic Contact

Nickel (Ni), the main responsible for allergic contact dermatitis worldwide, is also involved in systemic condition called “Systemic Nickel Sulfate Allergy Syndrome (SNAS).” Likewise, IgE-mediated reactivity to Lipid Transfer Protein (LTP) represents the main cause of primary food allergy in adults of Mediterranean countries. We evaluated the prevalence of SNAS in LTP allergic patients and investigated patients’ clinical features with double sensitization (LTP and Ni). A retrospective, single-center, observational study was conducted performing a complete allergological work-up including: (1) skin prick tests; (2) serum specific IgE for plant food allergens and rPru p3 (LTP); (3) patch test with 5% Ni sulfate in petrolatum. We enrolled 140 LTP allergic patients of which 36 patients (25.7% of sample) showed additional positivity to Ni patch test. Patients with double sensitization were more frequently females and reported fewer cutaneous symptoms. Higher values of sIgE for peach, apple, peanut, walnut, grain, corn, and garlic were found in LTP allergic patients, while higher values for hazelnut in the other subgroup. The prevalence of SNAS in the LTP allergic population is clinically relevant. Moreover, the clinical and immunological profiles of patients with double sensitization were different from patients monosensitized to LTP.

scholarly journals IgE Sensitization to the Nonspecific Lipid-Transfer Protein Ara h 9 and Peanut-Associated Bronchospasm

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Peter D. Arkwright ◽  
Colin W. Summers ◽  
Beverley J. Riley ◽  
Najla Alsediq ◽  
Richard S. H. Pumphrey
Keyword(s):  
Lipid Transfer Protein ◽  
Specific Ige ◽  
Rapid Onset ◽  
Research Database ◽  
Lipid Transfer ◽  
Transfer Protein ◽  
Skin Contact ◽  
Ara H 1 ◽  
History Of ◽  
Protein Components

Allergen component analysis is now available in many laboratories. The aim of this study was to examine the possible association between peanut allergen IgE components and severity of clinical reactions in patients with a history of peanut allergy. Data and sera collected from 192 patients within the Manchester Allergy Research Database and Serum Bank were used in this retrospective study. Sensitization to peanut specific IgE and Ara h 1, 2, 3, and 8 peanut IgE components, as measured by fluoroenzyme immunoassay, was not associated with anaphylaxis. In contrast, sensitization to the lipid-transfer protein Ara h 9 was significantly more prevalent in patients with peanut-associated bronchospasm (26% versus 9% of patients), even after adjusting for potential confounding effects of age, gender, and severity of concomitant chronic atopic diseases. Patients who were sensitized to Ara h 9 were more likely to have ingested rather than just have had skin contact with peanut and have a more rapid onset of symptoms. These results are consistent with observations that sensitization to heat and protease resistant lipid-transfer protein components of hazelnut, grains, and fruit is predictive of anaphylaxis.

scholarly journals A Case Report of Allergy to Peach’s Lipid Transfer Protein

2021 ◽  
pp. 22-25
Author(s):  
F. Yatribi ◽  
F. Benbrahim ◽  
N. Elhafidi ◽  
S. Benchekroun ◽  
C. Mahraoui
Keyword(s):  
Lipid Transfer Protein ◽  
Strong Association ◽  
Specific Ige ◽  
Skin Tests ◽  
Lipid Transfer ◽  
Heat Management ◽  
Transfer Protein ◽  
Primary Sensitization ◽  
The Difference ◽  
Pru P 3

Peach belongs to the Rosaceae family. One of the particularities of this allergy is the difference in clinical reactivity according to geography. We report a 4-year 8-month-old boy with well-controlled asthma and no symptoms of allergic rhinitis, that at the age of 1 year, 15 minutes after the ingestion of a fruit compote, presented urticaria with respiratory discomfort. At the age of 3 years, following ingestion of fruit salad, the child presented a few minutes later, a similar episode but of greater intensity. At the age of 4, following contact with a fruit basket, the child immediately presented a generalized reaction more severe and intense than the previous ones. Investigation of allergies to aerial allergens was negative. Skin tests for cooked and raw peach were positive for the peel and pulp. Specific-IgE was positive for Pru p 3 (5.7 KIU/l) and negative for Pru p 1. LTPs are particularly stable and resistant to proteolysis and heat. Management consisted of avoidance of peach, education of the child and family, and prescription of an emergency kit including self-injectable adrenaline. Our observation highlights the strong association between Pru p 3: peach’s Lipid Transfer Protein (LTP) positivity and the severity of allergy symptoms. We consider this case as a primary sensitization to peach’s LTP.

scholarly journals Beer, Cider, and Wine Allergy

2017 ◽  
Vol 2017 ◽  
pp. 1-4 ◽  
Author(s):  
Rhea A. Bansal ◽  
Susan Tadros ◽  
Amolak S. Bansal
Keyword(s):  
Lipid Transfer Protein ◽  
Specific Ige ◽  
Alcoholic Beverages ◽  
Food Groups ◽  
Lipid Transfer ◽  
Case Presentation ◽  
Blue Cheese ◽  
Transfer Protein ◽  
Ige Reactivity ◽  
Specific Proteins

Background. Allergy to beer is often due to specific proteins in barley and sometimes to lipid transfer protein. Allergy to wine is frequently due to a sensitivity to grape proteins. We present a rare case of allergy to beer, wine, and cider resulting from IgE reactivity to yeasts and moulds which also explained the patient’s additional sensitivity to yeast extracts and blue cheese. Case Presentation. The patient’s symptoms included throat and facial itching accompanied by mild wheeze and severe urticaria. Diagnosis of allergy to yeast was confirmed by specific IgE testing as well as that to relevant foods and beverages. The patient’s ongoing management included advice to avoid beer, wine, and other food groups containing specific yeasts, in addition to carrying a short acting nonsedating antihistamine as well as an adrenaline autoinjector. Conclusions. Cases of yeast allergy are extremely rare in medical literature but may be underrecognised and should be considered in patients presenting with reactions to alcoholic beverages and other yeast-containing products.

scholarly journals LTP Allergy Follow-Up Study: Development of Allergy to New Plant Foods 10 Years Later

Nutrients ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 2165
Author(s):  
Diana Betancor ◽  
Alicia Gomez-Lopez ◽  
Carlos Villalobos-Vilda ◽  
Emilio Nuñez-Borque ◽  
Sergio Fernández-Bravo ◽  
...  
Keyword(s):  
Food Allergy ◽  
Lipid Transfer Protein ◽  
Specific Ige ◽  
Cross Reactivity ◽  
Allergic Reactions ◽  
Plant Food ◽  
Lipid Transfer ◽  
Plant Foods ◽  
Transfer Protein

Introduction: Allergy to nonspecific lipid transfer protein (nsLTP) is the main cause of plant-food allergy in Spain. nsLTPs are widely distributed in the plant kingdom and have high cross-reactivity but extremely variable clinical expression. Little is known about the natural evolution of this allergy, which complicates management. The objective of this study was to assess the development of allergy to new plant foods in nsLTP-sensitized patients 10 years after diagnosis. Methods: One hundred fifty-one patients showing specific IgE to nsLTP determined by ISAC (Thermofisher) were included. After clinical workup (i.e., anamnesis, skin test, and challenge when needed), these patients were divided into two groups: 113 patients allergic to one or more plant food (74.5%) and 38 patients not allergic to any plant food (25.1%). Ten years later, a telephone interview was conducted to check whether patients had developed additional allergic reactions to plant foods. Results: Ten years after diagnosis, 35 of the 113 (31%) plant-food-allergic patients sensitized to nsLTP reported reactions to new, previously tolerated plant foods, mainly Rosaceae/Prunoideae fruits and nuts followed by vegetables, Rosacea/Pomoideae fruits, legumes, and cereals. Five out of 38 (13.2%) patients previously sensitized to nsLTP but without allergy to any plant food had experienced allergic reactions to some plant food: two to Rosaceae/Prunoideae fruits, two to Rosaceae/Prunoideae fruit and nuts, and one to legumes. Conclusion: Patients sensitized to nsLTP developed allergic reactions to other plant foods, mainly Rosaceae-Prunoideae fruits and nuts. This was more frequent among plant-food-allergic patients than among those who had never had plant-food allergy.

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