A Case Report of Allergy to Peach’s Lipid Transfer Protein

Peach belongs to the Rosaceae family. One of the particularities of this allergy is the difference in clinical reactivity according to geography. We report a 4-year 8-month-old boy with well-controlled asthma and no symptoms of allergic rhinitis, that at the age of 1 year, 15 minutes after the ingestion of a fruit compote, presented urticaria with respiratory discomfort. At the age of 3 years, following ingestion of fruit salad, the child presented a few minutes later, a similar episode but of greater intensity. At the age of 4, following contact with a fruit basket, the child immediately presented a generalized reaction more severe and intense than the previous ones. Investigation of allergies to aerial allergens was negative. Skin tests for cooked and raw peach were positive for the peel and pulp. Specific-IgE was positive for Pru p 3 (5.7 KIU/l) and negative for Pru p 1. LTPs are particularly stable and resistant to proteolysis and heat. Management consisted of avoidance of peach, education of the child and family, and prescription of an emergency kit including self-injectable adrenaline. Our observation highlights the strong association between Pru p 3: peach’s Lipid Transfer Protein (LTP) positivity and the severity of allergy symptoms. We consider this case as a primary sensitization to peach’s LTP.

Download Full-text

Lipid Transfer Protein Sensitization: Risk of Anaphylaxis and Molecular Sensitization Profile in Pru p 3-Sensitized Patients

International Archives of Allergy and Immunology ◽

10.1159/000511977 ◽

2020 ◽

pp. 1-8

Author(s):

Maria Ruano-Zaragoza ◽

Maria Luisa Somoza ◽

Teodorikez Wilfox Jiménez-Rodriguez ◽

Victor Soriano-Gomis ◽

Purificación González-Delgado ◽

...

Keyword(s):

Lipid Transfer Protein ◽

Allergic Diseases ◽

Specific Ige ◽

Oral Allergy Syndrome ◽

Skin Tests ◽

Food Allergies ◽

Lipid Transfer ◽

Cross Sectional ◽

Positive Skin ◽

Pru P 3

Background: Component-resolved diagnosis reveals the IgE response to many inhaled, food, and other allergens, improving the understanding and diagnosis of allergic diseases. Objective: The aims of the study are to study the recognition of different lipid transfer proteins (LTPs) and other allergen families in a large group of people sensitized to Pru p 3 and to analyze the relationship between the clinical entities and the allergens. Methods: This cross-sectional study included a large cohort of patients with positive skin tests to peach fruit and Pru p 3 specific IgE antibodies. Respiratory and food allergy symptoms were collected, and we performed prick tests with pollen, plant food, and other allergens plus the ImmunoCAP ISAC assay. Results: Our sample consisted of 421 people with a mean age of 33.25 years (range 16–68); 54.6% were women. Clinical entities included anaphylaxis (37.1%), urticaria (67.9%), and oral allergy syndrome (59.1%). Rhinitis, rhinoconjunctivitis, and/or asthma were diagnosed in 71.8% of the participants. The most pronounced correlation existed between sensitization to Pru p 3 and to Jug r 3, Pla a 3, Ara h 9, and Cor a 8. We found a higher incidence of anaphylaxis in people with 5 or more recognized LTPs. No association was observed between inhaled and food allergies. Conclusion: Most Pru p 3-sensitized participants were sensitized to additional allergens from the same family and, to a lesser extent, to other allergens, mainly in the profilin and PR-10 protein families. Anaphylaxis occurred in more than a third of the cases evaluated, and almost three-quarters of them had respiratory symptoms. Respiratory and food allergies involving LTPs do not seem to be associated.

Download Full-text

Pru p 3, a marker allergen for lipid transfer protein sensitization also in Central Europe

Allergy ◽

10.1111/all.13151 ◽

2017 ◽

Vol 72 (9) ◽

pp. 1415-1418 ◽

Cited By ~ 25

Author(s):

N. Mothes-Luksch ◽

M. Raith ◽

G. Stingl ◽

M. Focke-Tejkl ◽

E. Razzazi-Fazeli ◽

...

Keyword(s):

Central Europe ◽

Lipid Transfer Protein ◽

Lipid Transfer ◽

Transfer Protein ◽

Protein Sensitization ◽

Pru P 3

Download Full-text

Relationship between peach lipid transfer protein specific IgE levels and hypersensitivity to non-Rosaceae vegetable foods in patients allergic to lipid transfer protein

Annals of Allergy Asthma & Immunology ◽

10.1016/s1081-1206(10)61559-1 ◽

2004 ◽

Vol 92 (2) ◽

pp. 268-272 ◽

Cited By ~ 34

Author(s):

Riccardo Asero ◽

Gianni Mistrello ◽

Daniela Roncarolo ◽

Stefano Amato

Keyword(s):

Lipid Transfer Protein ◽

Specific Ige ◽

Lipid Transfer ◽

Transfer Protein ◽

Vegetable Foods

Download Full-text

In vitro gastrointestinal digestion of the major peach allergen Pru p 3, a lipid transfer protein: Molecular characterization of the products and assessment of their IgE binding abilities

Molecular Nutrition & Food Research ◽

10.1002/mnfr.200900552 ◽

2010 ◽

Vol 54 (10) ◽

pp. 1452-1457 ◽

Cited By ~ 27

Author(s):

Valeria Cavatorta ◽

Stefano Sforza ◽

Giancarlo Aquino ◽

Gianni Galaverna ◽

Arnaldo Dossena ◽

...

Keyword(s):

Molecular Characterization ◽

Lipid Transfer Protein ◽

Lipid Transfer ◽

Gastrointestinal Digestion ◽

Transfer Protein ◽

Ige Binding ◽

In Vitro Gastrointestinal Digestion ◽

Pru P 3

Download Full-text

Specific IgE Responses To Lipid Transfer Protein (LTP) And Profilin Have Interactive Effects On The Outcome Of Double-blind Placebo-controlled Food Challenges (DBPCFC) With Apple In Allergic Patients From Spain

Journal of Allergy and Clinical Immunology ◽

10.1016/j.jaci.2007.12.948 ◽

2008 ◽

Vol 121 (2) ◽

pp. S240-S240

Author(s):

M FERNANDEZRIVAS ◽

L BARREALES ◽

E GONZALEZMANCEBO ◽

L ZUIDMEER ◽

C MILLS ◽

...

Keyword(s):

Lipid Transfer Protein ◽

Specific Ige ◽

Interactive Effects ◽

Lipid Transfer ◽

Double Blind ◽

Double Blind Placebo ◽

Transfer Protein ◽

Food Challenges

Download Full-text

Structural and Functional Characterization of Recombinant Isoforms of the Lentil Lipid Transfer Protein

Acta Naturae ◽

10.32607/20758251-2015-7-3-65-73 ◽

2015 ◽

Vol 7 (3) ◽

pp. 65-73 ◽

Cited By ~ 6

Author(s):

I. V. Bogdanov ◽

E. I. Finkina ◽

S. V. Balandin ◽

D. N. Melnikova ◽

E. A. Stukacheva ◽

...

Keyword(s):

Disulfide Bonds ◽

Lipid Transfer Protein ◽

Functional Characterization ◽

Helical Structure ◽

Specific Ige ◽

Food Allergies ◽

Lipid Transfer ◽

E Coli ◽

Transfer Protein

The recombinant isoforms Lc-LTP1 and Lc-LTP3 of the lentil lipid transfer protein were overexpressed in E. coli cells. It was confirmed that both proteins are stabilized by four disulfide bonds and characterized by a high proportion of the -helical structure. It was found that Lc-LTP1 and Lc-LTP3 possess antimicrobial activity and can bind fatty acids. Both isoforms have the ability to bind specific IgE from sera of patients with food allergies, which recognize similar epitopes of the major peach allergen Pru p 3. Both isoforms were shown to have immunological properties similar to those of other plant allergenic LTPs, but Lc-LTP3 displayed a less pronounced immunoreactivity.

Download Full-text

Detection of Wheat Lipid Transfer Protein (Tri a 14) Sensitization: Comparison between Currently Available Diagnostic Tools

International Archives of Allergy and Immunology ◽

10.1159/000517963 ◽

2021 ◽

pp. 1-5

Author(s):

Clara San Bartolomé ◽

Rosa Muñoz-Cano ◽

Josefina Rius ◽

Rocío Casas-Saucedo ◽

Sara Balsells ◽

...

Keyword(s):

Lipid Transfer Protein ◽

Poor Performance ◽

Specific Ige ◽

Diagnostic Tools ◽

Lipid Transfer ◽

Prick Test ◽

Transfer Protein ◽

Whole Wheat ◽

Systemic Reactions ◽

Routine Diagnosis

Background: Wheat lipid transfer protein (LTP; Tri a 14) and ω5-gliadin have been described as major allergens in wheat allergy (WA) and relevant in wheat-induced anaphylaxis, frequently associated with cofactors. Objective: The objective of this study was to compare tools currently available in routine diagnosis to detect Tri a 14 sensitization, its clinical relevance, and cosensitization to ω5-gliadin and other LTPs. Methods: One hundred eighteen adults sensitized to rTri a 14 by ImmunoCAP® (cutoff ≥0.1 kUA/L) identified among 210 LTP allergic patients were included. We evaluated (1) wheat skin prick test (SPT), (2) specific IgE (sIgE) to wheat, rTri a 14, rTri a 19, peach, apple, walnut, hazelnut, and peanut LTPs using ImmunoCAP® and microarray ImmunoCAP®ISAC (cutoff ≥0.3I SU), and (3) wheat-related symptoms. Results: Wheat SPT and sIgE were positive in 31% and 85% of subjects, respectively. rTri a 14 by microarray was detected in 25%. Eight percent showed cosensitization to ω5-gliadin. Thirty percent referred symptoms (gastrointestinal [13%], urticaria [11%], and anaphylaxis [8%]). Cofactors (45%) were significantly associated with systemic reactions. Conclusion: WA due to Tri a 14 is frequently related with systemic reactions and because are frequently related to cofactors, the culprit may not be suspected. Together with the poor performance to identify Tri a 14 sensitization of the current routine diagnostic tools based on the analysis of whole wheat extract, such as wheat SPT or sIgE, there is a high risk that WA may be overlooked. Thus, when WA is suspected, sIgE Tri a 14 assessment is recommended, together with wheat and ω5-gliadin, preferably in the singleplex format, and carefully evaluated considering ≥0.1 kUA/L as a cutoff.

Download Full-text