scholarly journals Dose Optimization of Calcusol™ and Calcium Oxalate Monohydrate (COM) on Primary Renal Epithelial Cells Cultures of Mice (Mus musculus)

2014 ◽  
Vol 4 (2) ◽  
pp. 101-105
Author(s):  
Ahmad Soni ◽  
Muhammad Sasmito Djati ◽  
Sri Widyarti
Keyword(s):  
Epithelial Cells ◽  
Calcium Oxalate ◽  
Mus Musculus ◽  
Calcium Oxalate Monohydrate ◽  
Dose Optimization ◽  
Renal Epithelial Cells

Kidney stone matrix proteins ameliorate calcium oxalate monohydrate induced apoptotic injury to renal epithelial cells

Life Sciences ◽  
2016 ◽  
Vol 164 ◽  
pp. 23-30 ◽  
Author(s):  
Shifa Narula ◽  
Simran Tandon ◽  
Shrawan Kumar Singh ◽  
Chanderdeep Tandon
Keyword(s):  
Epithelial Cells ◽  
Calcium Oxalate ◽  
Kidney Stone ◽  
Calcium Oxalate Monohydrate ◽  
Matrix Proteins ◽  
Renal Epithelial Cells ◽  
Stone Matrix

scholarly journals Calcium oxalate monohydrate crystals stimulate gene expression in renal epithelial cells

1995 ◽  
Vol 48 (2) ◽  
pp. 501-509 ◽  
Author(s):  
Mary S. Hammes ◽  
John C. Lieske ◽  
Shashi Pawar ◽  
Benjamin H. Spargo ◽  
F. Gary Toback
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Calcium Oxalate ◽  
Calcium Oxalate Monohydrate ◽  
Renal Epithelial Cells

scholarly journals Total flavones of Desmodium styracifolium antagonize calcium oxalate monohydrate-triggered IL-2Rβ expression in renal epithelial cells

2021 ◽  
Vol 18 (5) ◽  
pp. 1077-1081
Author(s):  
Haijie Xie ◽  
Rui Yang ◽  
Yong Xu ◽  
Chunyu Liu
Keyword(s):  
Epithelial Cells ◽  
Western Blot ◽  
Calcium Oxalate ◽  
Human Kidney ◽  
Calcium Oxalate Monohydrate ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dependent Manner ◽  
Renal Epithelial Cells ◽  
Desmodium Styracifolium ◽  
Proximal Tubular

Purpose: To explore the effect of total flavone of Desmodium styracifolium (TFDS) on calcium oxalate monohydrate (COM)-triggered IL-2Rβ expression in human kidney proximal tubular epithelial cells. Methods: Human kidney proximal tubular epithelial cell line HK-2 was treated with COM, TFDS or both. The expression of IL-2Rβ was evaluated by quantitative polymerase chain reaction (qPCR) or flow cytometry. The responsiveness of HK-2 cells to IL-2 was determined by enzyme-linked immunosorbent assay (ELISA), qPCR and western blot. The signaling mechanism underlying the effect of TFDS was studied using western blot and qPCR. The clinical relevance of IL-2Rβ to renal inflammation was investigated by re-analyzing a Gene Expression Omnibus (GEO) dataset. Results: Total flavones of Desmodium styracifolium (TFDS) antagonize COM-triggered IL-2Rβ expression in HK-2 cells, thus reducing the responsiveness of HK-2 cells to IL-2 stimulation. Mechanistically, TFDS dampens IL-2Rβ expression by preventing the activation of STAT3. The level of IL-2Rβ is positively correlated with the inflammatory status of the kidney. Conclusions: The total flavones of Desmodium styracifolium (TFDS) prevent the upregulation of IL2Rβ in renal epithelial cells upon COM stimulation in a STAT3-dependent manner.

scholarly journals Adhesion, internalization and metabolism of calcium oxalate monohydrate crystals by renal epithelial cells

1997 ◽  
Vol 52 (5) ◽  
pp. 1291-1301 ◽  
Author(s):  
John C. Lieske ◽  
Rebbecca Norris ◽  
Hewson Swift ◽  
F. Gary Toback
Keyword(s):  
Epithelial Cells ◽  
Calcium Oxalate ◽  
Calcium Oxalate Monohydrate ◽  
Renal Epithelial Cells

Adhesion of calcium oxalate monohydrate crystals to renal epithelial cells is inhibited by specific anions

1995 ◽  
Vol 268 (4) ◽  
pp. F604-F612 ◽  
Author(s):  
J. C. Lieske ◽  
R. Leonard ◽  
F. G. Toback
Keyword(s):  
Epithelial Cells ◽  
Cell Surface ◽  
Calcium Oxalate ◽  
Chondroitin Sulfate ◽  
Kidney Stones ◽  
Calcium Oxalate Monohydrate ◽  
Apical Surface ◽  
Dependent Manner ◽  
Renal Epithelial Cells ◽  
Renal Tubular Cells

Adhesion of urinary crystals to the apical surface of renal tubular cells could be a critical step in the formation of kidney stones. The interaction between renal epithelial cells (BSC-1 line) and the most common crystal in kidney stones, calcium oxalate monohydrate (COM), was studied in a tissue culture model system. COM crystals bound to the cell surface within seconds in a concentration-dependent manner to a far greater extent than did brushite, another calcium-containing crystal found in urine. Adhesion of COM crystals to cells was blocked by the polyanion, heparin. Other glycosaminoglycans including chondroitin sulfate A or B, heparan sulfate, and hyaluronic acid, but not chondroitin sulfate C, prevented binding of COM crystals. Two nonsulfated polyanions, polyglutamic acid and polyaspartic acid, also blocked adherence of COM crystals. Three molecules found in urine, nephrocalcin, uropontin, and citrate, each inhibited binding of COM crystals, whereas Tamm-Horsfall glycoprotein (THP) did not. Prior exposure of crystals but not cells to inhibitory molecules blocked adhesion, suggesting that these agents exert their effect at the crystal surface. Inhibition of crystal binding followed a linear Langmuir adsorption isotherm for each inhibitor identified, suggesting that these molecules bind to a single class of sites on the crystal that are important for adhesion to the cell surface. Inhibition of crystal adhesion by heparin was rapidly overcome by the polycation protamine, suggesting that the glycosaminoglycan regulates cell-crystal interactions in a potentially reversible manner.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Renal epithelial cells rapidly bind and internalize calcium oxalate monohydrate crystals.

1994 ◽  
Vol 91 (15) ◽  
pp. 6987-6991 ◽  
Author(s):  
J. C. Lieske ◽  
H. Swift ◽  
T. Martin ◽  
B. Patterson ◽  
F. G. Toback
Keyword(s):  
Epithelial Cells ◽  
Calcium Oxalate ◽  
Calcium Oxalate Monohydrate ◽  
Renal Epithelial Cells
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