Total flavones of Desmodium styracifolium antagonize calcium oxalate monohydrate-triggered IL-2Rβ expression in renal epithelial cells

Purpose: To explore the effect of total flavone of Desmodium styracifolium (TFDS) on calcium oxalate monohydrate (COM)-triggered IL-2Rβ expression in human kidney proximal tubular epithelial cells. Methods: Human kidney proximal tubular epithelial cell line HK-2 was treated with COM, TFDS or both. The expression of IL-2Rβ was evaluated by quantitative polymerase chain reaction (qPCR) or flow cytometry. The responsiveness of HK-2 cells to IL-2 was determined by enzyme-linked immunosorbent assay (ELISA), qPCR and western blot. The signaling mechanism underlying the effect of TFDS was studied using western blot and qPCR. The clinical relevance of IL-2Rβ to renal inflammation was investigated by re-analyzing a Gene Expression Omnibus (GEO) dataset. Results: Total flavones of Desmodium styracifolium (TFDS) antagonize COM-triggered IL-2Rβ expression in HK-2 cells, thus reducing the responsiveness of HK-2 cells to IL-2 stimulation. Mechanistically, TFDS dampens IL-2Rβ expression by preventing the activation of STAT3. The level of IL-2Rβ is positively correlated with the inflammatory status of the kidney. Conclusions: The total flavones of Desmodium styracifolium (TFDS) prevent the upregulation of IL2Rβ in renal epithelial cells upon COM stimulation in a STAT3-dependent manner.

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Adhesion of calcium oxalate monohydrate crystals to renal epithelial cells is inhibited by specific anions

AJP Renal Physiology ◽

10.1152/ajprenal.1995.268.4.f604 ◽

1995 ◽

Vol 268 (4) ◽

pp. F604-F612 ◽

Cited By ~ 36

Author(s):

J. C. Lieske ◽

R. Leonard ◽

F. G. Toback

Keyword(s):

Epithelial Cells ◽

Cell Surface ◽

Calcium Oxalate ◽

Chondroitin Sulfate ◽

Kidney Stones ◽

Calcium Oxalate Monohydrate ◽

Apical Surface ◽

Dependent Manner ◽

Renal Epithelial Cells ◽

Renal Tubular Cells

Adhesion of urinary crystals to the apical surface of renal tubular cells could be a critical step in the formation of kidney stones. The interaction between renal epithelial cells (BSC-1 line) and the most common crystal in kidney stones, calcium oxalate monohydrate (COM), was studied in a tissue culture model system. COM crystals bound to the cell surface within seconds in a concentration-dependent manner to a far greater extent than did brushite, another calcium-containing crystal found in urine. Adhesion of COM crystals to cells was blocked by the polyanion, heparin. Other glycosaminoglycans including chondroitin sulfate A or B, heparan sulfate, and hyaluronic acid, but not chondroitin sulfate C, prevented binding of COM crystals. Two nonsulfated polyanions, polyglutamic acid and polyaspartic acid, also blocked adherence of COM crystals. Three molecules found in urine, nephrocalcin, uropontin, and citrate, each inhibited binding of COM crystals, whereas Tamm-Horsfall glycoprotein (THP) did not. Prior exposure of crystals but not cells to inhibitory molecules blocked adhesion, suggesting that these agents exert their effect at the crystal surface. Inhibition of crystal binding followed a linear Langmuir adsorption isotherm for each inhibitor identified, suggesting that these molecules bind to a single class of sites on the crystal that are important for adhesion to the cell surface. Inhibition of crystal adhesion by heparin was rapidly overcome by the polycation protamine, suggesting that the glycosaminoglycan regulates cell-crystal interactions in a potentially reversible manner.(ABSTRACT TRUNCATED AT 250 WORDS)

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Injury Difference of the Un-Aggregated and Aggregated Calcium Oxalate Monohydrate on Human Kidney Proximal Tubular Epithelial Cells

Proceedings of the 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017) ◽

10.2991/bbe-17.2017.26 ◽

2017 ◽

Author(s):

Li-shan HUANG ◽

Da GUO ◽

Jian-ming OUYANG

Keyword(s):

Epithelial Cells ◽

Calcium Oxalate ◽

Human Kidney ◽

Calcium Oxalate Monohydrate ◽

Tubular Epithelial Cells ◽

Proximal Tubular Epithelial Cells ◽

Proximal Tubular

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Repair of Tea Polysaccharide Promotes the Endocytosis of Nanocalcium Oxalate Monohydrate by Damaged HK-2 Cells

Oxidative Medicine and Cellular Longevity ◽

10.1155/2020/2198976 ◽

2020 ◽

Vol 2020 ◽

pp. 1-12

Author(s):

Chuang-Ye Li ◽

Li Liu ◽

Yao-Wang Zhao ◽

Qian-Long Peng ◽

Xin-Yuan Sun ◽

...

Keyword(s):

Human Kidney ◽

Calcium Oxalate Monohydrate ◽

Protective Mechanism ◽

Renal Epithelial Cells ◽

Molecular Weights ◽

Lactate Dehydrogenase Release ◽

Before And After ◽

Proximal Tubular ◽

Repair Ability ◽

Tea Polysaccharides

Endocytosis is a protective mechanism of renal epithelial cells to eliminate retained crystals. This research investigated the endocytosis of 100 nm calcium oxalate monohydrate crystals in human kidney proximal tubular epithelial (HK-2) cells before and after repair by four kinds of tea polysaccharides with molecular weights (MWs) of 10.88 (TPS0), 8.16 (TPS1), 4.82 (TPS2), and 2.31 kDa (TPS3), respectively. When HK-2 cells were repaired by TPSs after oxalic acid injury, the cell viability, wound healing ability, mitochondrial membrane potential, percentage of cells with endocytosed crystals, and dissolution rate of the endocytosed crystals increased; the cell morphology recovered; and the reactive oxygen level and lactate dehydrogenase release decreased. Most of the endocytosed crystals were found in the lysosomes. The repair effects of the four TPSs were ranked in the following order: TPS2>TPS1>TPS3>TPS0. TPS2 with moderate MW presented the optimal repair ability and strongest ability to promote endocytosis.

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