scholarly journals ОПИС КЛІНІЧНОГО ВИПАДКУ ЛАЙМ-КАРДИТУ

2021 ◽  
pp. 166-172
Author(s):  
Н. І. Ярема ◽  
К. В. Миндзів ◽  
О. І. Коцюба ◽  
Д. В. Попович
Keyword(s):  
Borrelia Burgdorferi ◽  
Lyme Carditis

Мета роботи – на прикладі клінічного випадку Лайм-кардиту продемонструвати особливості діагностичного процесу з визначенням факторів ризику, клінічних і ЕКГ-змін, важливість своєчасної верифікації діагнозу і проведення етіотропної терапії. У пацієнта виявлені складні порушення ритму та провідності: AV-блокада І ст., транзиторні AV-блокада ІІ (Мобітц 2) та ІІІ ступенів, політопна екстрасистолія. Дані лабораторних обстежень пацієнта свідчили про підвищення маркерів запалення (підвищення рівнів СРП, тропоніну та ШОЕ). Для оцінки ймовірності бореліозної етіології міокардиту проведене анкетування за шкалою SILC (Suspicious Index in Lyme Carditis) – 8 балів, що вказує на високий ризик Лайм-кардиту, серологічне обстеження. Блот-аналіз підтвердив наявність антитіл IgG до Borrelia burgdorferi. Завдяки своєчасній діагностиці, в тому числі серологічній, Лайм-кардиту і призначеному відповідному етіотропному антибактеріальному лікуванню доксицикліном впродовж 21 дня, у хворого було досягнуто як суттєвого клінічного покращення, так і позитивної ЕКГ-динаміки з покращенням AV-провідності.

scholarly journals DETERMINING AN APPROPRIATE MURINE MODEL FOR STUDYING THE PATHOLOGICAL RESPONSE TO BORRELIA BURGDORFERI IN DEVELOPING LYME CARDITIS

2021 ◽  
Vol 77 (18) ◽  
pp. 652
Author(s):  
Veronica N. Harrison ◽  
Catherine Brissette ◽  
Timothy Casselli ◽  
Derick Thompson ◽  
Heidi Pecoraro ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Murine Model ◽  
Pathological Response ◽  
Lyme Carditis

scholarly journals A Case of Lyme Carditis Presenting with Atrial Fibrillation

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Peter J. Kennel ◽  
Melvin Parasram ◽  
Daniel Lu ◽  
Diane Zisa ◽  
Samuel Chung ◽  
...  
Keyword(s):  
Atrial Fibrillation ◽  
Borrelia Burgdorferi ◽  
Antibiotic Therapy ◽  
Unusual Presentation ◽  
Type I ◽  
Atrial Arrhythmias ◽  
Av Block ◽  
Lyme Carditis ◽  
Second Degree

We report a case of a 20-year-old man who presented to our institution with a new arrhythmia on a routine EKG. Serial EKG tracings revealed various abnormal rhythms such as episodes of atrial fibrillation, profound first degree AV block, and type I second degree AV block. He was found to have positive serologies for Borrelia burgdorferi. After initiation of antibiotic therapy, the atrial arrhythmias and AV block resolved. Here, we present a case of Lyme carditis presenting with atrial fibrillation, a highly unusual presentation of Lyme carditis.

scholarly journals Evaluation of Selected Borrelia burgdorferi lp54 Plasmid-Encoded Gene Products Expressed during Mammalian Infection as Antigens To Improve Serodiagnostic Testing for Early Lyme Disease

2015 ◽  
Vol 22 (11) ◽  
pp. 1176-1186 ◽  
Author(s):  
Zachary P. Weiner ◽  
Rebecca M. Crew ◽  
Kevin S. Brandt ◽  
Amy J. Ullmann ◽  
Martin E. Schriefer ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Recombinant Proteins ◽  
Erythema Migrans ◽  
Disease Patient ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Early Stages ◽  
Lyme Carditis ◽  
Mammalian Infection

ABSTRACTLaboratory testing for the diagnosis of Lyme disease is performed primarily by serologic assays and is accurate for detection beyond the acute stage of the infection. Serodiagnostic assays to detect the early stages of infection, however, are limited in their sensitivity, and improvement is warranted. We analyzed a series ofBorrelia burgdorferiproteins known to be induced within feeding ticks and/or during mammalian infection for their utility as serodiagnostic markers against a comprehensive panel of Lyme disease patient serum samples. The antigens were assayed for IgM and IgG reactivity in line immunoblots and separately by enzyme-linked immunosorbent assay (ELISA), with a focus on reactivity against early Lyme disease with erythema migrans (EM), early disseminated Lyme neuroborreliosis, and early Lyme carditis patient serum samples. By IgM immunoblotting, we found that recombinant proteins BBA65, BBA70, and BBA73 reacted with early Lyme EM samples at levels comparable to those of the OspC antigen used in the current IgM blotting criteria. Additionally, these proteins reacted with serum samples from patients with early neuroborreliosis and early carditis, suggesting value in detecting early stages of this disease progression. We also found serological reactivity against recombinant proteins BBA69 and BBA73 with early-Lyme-disease samples using IgG immunoblotting and ELISA. Significantly, some samples that had been scored negative by the Centers for Disease Control and Prevention-recommended 2-tiered testing algorithm demonstrated positive reactivity to one or more of the antigens by IgM/IgG immunoblot and ELISA. These results suggest that incorporating additionalin vivo-expressed antigens into the current IgM/IgG immunoblotting tier in a recombinant protein platform assay may improve the performance of early-Lyme-disease serologic testing.

scholarly journals Borrelia burgdorferi bbk13Is Critical for Spirochete Population Expansion in the Skin during Early Infection

2019 ◽  
Vol 87 (5) ◽  
Author(s):  
George F. Aranjuez ◽  
Hunter W. Kuhn ◽  
Philip P. Adams ◽  
Mollie W. Jewett
Keyword(s):  
Borrelia Burgdorferi ◽  
Population Expansion ◽  
Targeted Mutagenesis ◽  
Lyme Arthritis ◽  
Content Type ◽  
Lyme Carditis ◽  
Inoculation Site ◽  
Full Complement ◽  
Mammalian Infection ◽  
Mouse Model Of Infection

ABSTRACTLyme disease is caused by the spirocheteBorrelia burgdorferiand is transmitted via the bite of an infected tick.B. burgdorferienters the skin, disseminates via the bloodstream, and infects various distal tissues, leading to inflammatory sequelae, such as Lyme arthritis and Lyme carditis.B. burgdorferilinear plasmid 36 (lp36) is critical for mammalian infectivity; however, the full complement of genes on lp36 that contribute to this process remains unknown. Through a targeted mutagenesis screen of the genes on lp36, we identified a novel infectivity gene of unknown function,bbk13, which encodes an immunogenic, non-surface-exposed membrane protein that is important for efficient mammalian infection. Loss ofbbk13resulted in reduced spirochete loads in distal tissues in a mouse model of infection. Through a detailed analysis ofB. burgdorferiinfection kinetics, we discovered thatbbk13is important for promoting spirochete proliferation in the skin inoculation site. The attenuated ability of Δbbk13spirochetes to proliferate in the inoculation site was followed by reduced numbers ofB. burgdorferispirochetes in the bloodstream and, ultimately, consistently reduced spirochete loads in distal tissues. Together, our data indicate thatbbk13contributes to disseminated infection by promoting spirochete proliferation in the early phase of infection in the skin. This work not only increases the understanding of the contribution of the genes on lp36 toB. burgdorferiinfection but also begins to define the genetic basis forB. burgdorferiexpansion in the skin during localized infection and highlights the influence of the early expansion of spirochetes in the skin on the outcome of infection.

scholarly journals Borrelia burgdorferi infection induces long-term memory-like responses in macrophages with tissue-wide consequences in the heart

PLoS Biology ◽  
2021 ◽  
Vol 19 (1) ◽  
pp. e3001062
Author(s):  
Diego Barriales ◽  
Itziar Martín-Ruiz ◽  
Ana Carreras-González ◽  
Marta Montesinos-Robledo ◽  
Mikel Azkargorta ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Inflammatory Responses ◽  
Long Term Memory ◽  
Term Memory ◽  
Lyme Carditis ◽  
Extracutaneous Manifestation ◽  
Live Bacteria

Lyme carditis is an extracutaneous manifestation of Lyme disease characterized by episodes of atrioventricular block of varying degrees and additional, less reported cardiomyopathies. The molecular changes associated with the response to Borrelia burgdorferi over the course of infection are poorly understood. Here, we identify broad transcriptomic and proteomic changes in the heart during infection that reveal a profound down-regulation of mitochondrial components. We also describe the long-term functional modulation of macrophages exposed to live bacteria, characterized by an augmented glycolytic output, increased spirochetal binding and internalization, and reduced inflammatory responses. In vitro, glycolysis inhibition reduces the production of tumor necrosis factor (TNF) by memory macrophages, whereas in vivo, it produces the reversion of the memory phenotype, the recovery of tissue mitochondrial components, and decreased inflammation and spirochetal burdens. These results show that B. burgdorferi induces long-term, memory-like responses in macrophages with tissue-wide consequences that are amenable to be manipulated in vivo.

Een sportieve vijftiger met brutale syncope ten gevolge van Lyme carditis

2021 ◽  
Author(s):  
C. BAESTAENS ◽  
S. HELLEMANS
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Antibiotic Therapy ◽  
Medical History ◽  
Permanent Pacemaker ◽  
Serological Testing ◽  
Conduction Disturbances ◽  
Lyme Carditis ◽  
Life Threatening ◽  
Life Threatening Complication

A sportive man in his 50's from Philadelphia with a brutal syncope from Lyme carditis In the case of a syncope at a young age in a patient with no medical history - especially if he or she comes from endemic regions - Lyme disease should be excluded through serological testing. Lyme carditis is a potentially life-threatening complication after infection with the bacterium Borrelia burgdorferi. This is shown in the discussed case of a 51-year-old sportive man who registered for a consultation after a brutal syncope. Under antibiotic therapy, the severe conduction disturbances progressively cleared up and a permanent pacemaker could be avoided.

New ultrastructural findings about Borrelia burgdorferi by cryofixation, negative staining, thin sectioning and scanning techniques

1990 ◽  
Vol 48 (3) ◽  
pp. 582-583
Author(s):  
S. F. Hayes ◽  
M. D. Corwin ◽  
T. G. Schwan ◽  
D. W. Dorward ◽  
W. Burgdorfer
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Structural Characterization ◽  
Negative Staining ◽  
Low Speed ◽  
Thin Sectioning ◽  
Ultrastructural Findings

Characterization of Borrelia burgdorferi strains by means of negative staining EM has become an integral part of many studies related to the biology of the Lyme disease organism. However, relying solely upon negative staining to compare new isolates with prototype B31 or other borreliae is often unsatisfactory. To obtain more satisfactory results, we have relied upon a correlative approach encompassing a variety EM techniques, i.e., scanning for topographical features and cryotomy, negative staining and thin sectioning to provide a more complete structural characterization of B. burgdorferi.For characterization, isolates of B. burgdorferi were cultured in BSK II media from which they were removed by low speed centrifugation. The sedimented borrelia were carefully resuspended in stabilizing buffer so as to preserve their features for scanning and negative staining. Alternatively, others were prepared for conventional thin sectioning and for cryotomy using modified procedures. For thin sectioning, the fixative described by Ito, et al.

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