scholarly journals Importance of extending the use of Polymerase chain reaction in the diagnosis of Venereal syphilis in a blood transfusion center in Burkina Faso, West Africa

2014 ◽  
Vol 18 ◽  
Author(s):  
Abibou Simpore ◽  
Cyrille Bisseye ◽  
Bolni Marius Nagalo ◽  
Mahamoudou Sanou ◽  
Yacouba Nébié ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Blood Transfusion ◽  
West Africa ◽  
Burkina Faso ◽  
Chain Reaction ◽  
Transfusion Center ◽  
Blood Transfusion Center ◽  
Polymerase Chain

scholarly journals Molecular and Pathotypic Characterization of New Xanthomonas oryzae Strains from West Africa

2007 ◽  
Vol 20 (5) ◽  
pp. 534-546 ◽  
Author(s):  
Carolina Gonzalez ◽  
Boris Szurek ◽  
Charles Manceau ◽  
Thierry Mathieu ◽  
Yacouba Séré ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
West Africa ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Xanthomonas Oryzae ◽  
Polymorphism Analysis ◽  
Transcription Activator ◽  
Chain Reaction ◽  
Polymerase Chain

DNA polymorphism analysis and pathogenicity assays were used to characterize strains of Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola collected from rice leaves in West Africa. Restriction fragment length polymorphism (RFLP), repetitive sequence-based polymerase chain reaction, fluorescent amplified fragment-length polymorphism (FAFLP) analyses were assessed for molecular characterization, while pathogenicity was tested by leaf clipping and leaf infiltration. Dendrograms were generated for the data sets obtained from RFLP analysis and repetitive polymerase chain reaction suggesting that the interrelationships between strains were dependent on the technique used. In all cases, data showed that African strains of X. oryzae pv. oryzae form a group genetically distant from Asian strains. FAFLP analyses separated the X. oryzae strains into three groups with significant bootstrap values. A specific and intriguing feature of African strains of X. oryzae pv. oryzae is a reduction in the number of insertion sequence elements and transcription activator-like (avrBs3/pthA) effector genes, based on the molecular markers employed in the study. In addition, pathogenicity assays conducted with African strains of X. oryzae pv. oryzae on a series of nearly isogenic lines (NILs) identified three new races. Finally, leaf infiltration assays revealed the capacity of African strains of X. oryzae pv. oryzae to induce a nonhost hypersensitive response in Nicotiana benthamiana, in contrast with Asian X. oryzae pv. oryzae and X. oryzae pv. oryzicola strains. Our results reveal substantial differences between genomic characteristics of Asian and African strains of X. oryzae pv. oryzae.

Polymerase chain reaction as a diagnosis tool for detecting trypanosomes in naturally infected cattle in Burkina Faso

1999 ◽  
Vol 86 (2) ◽  
pp. 95-103 ◽  
Author(s):  
P Solano ◽  
J.F Michel ◽  
T Lefrançois ◽  
S de La Rocque ◽  
I Sidibé ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Burkina Faso ◽  
Infected Cattle ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Diagnosis Tool

scholarly journals Genotyping of Human Platelet Antigen-1 to -5 and -15 by Polymerase Chain Reaction with Sequence-specific Primers (PCR-SSP) and Real-time PCR in Azeri Blood Donors

2021 ◽  
Author(s):  
Seyed Ghader Azizi ◽  
Shahram Samiee ◽  
Mojgan Shaiegan ◽  
Maryam Zadsar
Keyword(s):  
Polymerase Chain Reaction ◽  
Blood Transfusion ◽  
Real Time ◽  
Real Time Pcr ◽  
Blood Donors ◽  
Human Platelet ◽  
Chain Reaction ◽  
Specific Primers ◽  
Nucleotide Mutation ◽  
Polymerase Chain

Human platelet antigens (HPAs) are glycoproteins on the platelet surface that a single nucleotide mutation in the coding region gene could lead to the variation of different HPA polymorphisms. These antigens have shown variation among different races and may trigger immune responses during blood transfusion and pregnancy. Genotyping of HPAs is useful for managing these reactions and establishing a platelet registry to decrease platelet transfusion reactions. This study aimed to compare allelic and genotype frequencies of human platelet antigens in the Azeri ethnicity by TaqMan Real-time and polymerase chain reaction with sequence-specific primers (PCR-SSP) methods. DNA was extracted from the whole blood of 100 Azeri blood donors in the Ardabil Blood Transfusion Center. Genotyping of HPA-1 to -5 and -15 was performed by TaqMan Real-time PCR, and PCR-SSP and consistency of results were evaluated. The results of PCR-SSP and TaqMan Real-time PCR showed complete consistency. The allele frequencies were 91.5% and 8.5% for HPA-1a and -1b; 88% and 12% for HPA-2a and -2b; 58% and 42 % for HPA-3a and -3b; 100% for HPA-4a; 91% and 9% for HPA-5a and -5b; 56.5% and 43.5% for HPA-15a and -15b alleles. Not incompatibility was detected in HPAs genotyping by PCR-SSP and TaqMan Real-time PCR so that real-time PCR can be used as a robust and quick method for HPA genotyping. We found differences between Azeri blood donors and previously reported HPAs alleles’ frequency in other ethnicities in the country. This fact highlights the need for a platelet registry to recruit platelet donors from different ethnicities and increase the number of donors by using faster methods.

Trypanosome characterization by polymerase chain reaction in Glossina palpalis gambiensisand G.tachinoidesfrom Burkina Faso

1996 ◽  
Vol 10 (4) ◽  
pp. 354-358 ◽  
Author(s):  
P. SOLANO ◽  
J. M. REIFENBERG ◽  
S. AMSLER-DELAFOSSE ◽  
I. KABORE ◽  
D. CUISANCE ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Burkina Faso ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Glossina Palpalis
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