scholarly journals Role of LDL receptors in the in vitro uptake and degradation of LDL in the media of rabbit thoracic aorta.

1989 ◽  
Vol 64 (5) ◽  
pp. 957-967 ◽  
Author(s):  
P A Curmi ◽  
G Renaud ◽  
L Juan ◽  
B Chiron ◽  
A Tedgui
Keyword(s):  
Thoracic Aorta ◽  
Ldl Receptors ◽  
The Media ◽  
In Vitro Uptake

On the Role of Transferrin in 67Ga Uptake by Tumor Cells

1988 ◽  
Vol 27 (04) ◽  
pp. 151-153
Author(s):  
P. Thouvenot ◽  
F. Brunotte ◽  
J. Robert ◽  
L. J. Anghileri
Keyword(s):  
Specific Binding ◽  
Subcellular Fractionation ◽  
Animal Blood ◽  
Tumor Bearing ◽  
Cell Structures ◽  
The Difference ◽  
Sarcoma Cells ◽  
In Vitro Uptake

In vitro uptake of 67Ga-citrate and 59Fe-citrate by DS sarcoma cells in the presence of tumor-bearing animal blood plasma showed a dramatic inhibition of both 67Ga and 59Fe uptakes: about ii/io of 67Ga and 1/5o of the 59Fe are taken up by the cells. Subcellular fractionation appears to indicate no specific binding to cell structures, and the difference of binding seems to be related to the transferrin chelation and transmembrane transport differences

Role of gibberellic acid in cotton fibre development

2002 ◽  
Vol 138 (3) ◽  
pp. 255-260 ◽  
Author(s):  
S. J. GOKANI ◽  
V. S. THAKER
Keyword(s):  
Gibberellic Acid ◽  
Fibre Length ◽  
Dry Weight ◽  
Cotton Fibre ◽  
Growth Phases ◽  
Fibre Development ◽  
The Media

Fibres of three cotton cultivars (Gossypium hirsutum H-4, H-8 and G. arboreum G. Cot-15) were analysed for growth in terms of fibre length and dry weight and endogenous gibberellic acid (GA3) content thrice during 1997–2000, at Rajkot. The development of cotton fibre was divided into four distinct growth phases but overlap between elongation and secondary thickening was considerable which suggests that both these phases are independent of each other. During fibre elongation, GA3 content remained low and increased after a decrease in the rate of fibre elongation in all three genotypes. The long staple cultivar (H-4) showed highest endogenous GA3 content followed by the middle one (H-8) and the short staple cultivar (G. Cot-15). In in vitro studies when GA3, NAA or GA3+NAA was supplemented to the media, increase in fibre length of the short staple cultivar was maximum, followed by the middle one and the long staple cultivar. Both in vivo and in vitro findings suggest that GA3 is one of the important factors that determine fibre length.

scholarly journals Estrogen Differentially Regulates Protein Abundance in Exosomes Released From Immortalized Kisspeptin Neurons in Vitro

2021 ◽  
Vol 5 (Supplement_1) ◽  
pp. A538-A538
Author(s):  
Teagan James ◽  
Patrick Everett Chappell
Keyword(s):  
Estrogen Receptor Alpha ◽  
Bone Matrix ◽  
Dependent Manner ◽  
Alizarin Red ◽  
Runx2 Expression ◽  
Skeletal Homeostasis ◽  
The Media ◽  
Osteogenic Factors

Abstract Estrogen (E2) is essential for multiple physiological effects in females, ensuring maximum reproductive fitness and maintaining skeletal homeostasis. E2 has been shown to stimulate cancellous bone formation via activation of estrogen receptor alpha (ERα), an effect widely accepted to be mediated directly at bone. A recent landmark study (Herber et al., Nat Commun 2019) demonstrated bone density increases in female mice harboring ERα-deletions specifically in arcuate Kiss-1 neurons. In this study, bone from transgenic females showed higher osteoblast functioning and increases in the expression of sp7 and runx2, positing a direct neural-bone regulatory axis altered by circulating E2 acting in brain. Our laboratory has used two immortalized Kisspeptin (Kiss1)-expressing and -secreting cell lines, KTaR-1 (representative of female arcuate Kiss-1 neurons) and KTaV-3 cells (representative of female AVPV Kiss-1 neurons) as models to explore the role of Kiss-1 in multiple physiological regulatory contexts. We recently determined that factors in the media of female ARC-derived KTaR-1 cells can affect parameters of osteoblast function in vitro, including increases in sp7 and runx2 expression, and formation of bone matrix (evaluated by Alizarin Red assay). Exposure of canine osteosarcoma cells to conditioned media from KTaR-1 cells led to increases in sp7 expression in an E2-dependent manner, and 24h E2-deprivation of these neurons stimulated secretion of osteogenic factors. In this current study, we have used LCMS-MS proteomic analysis to determine the contents of exosomes isolated from Kisspeptin neurons under varying E2 exposure conditions in vitro. Preliminary results reveal ~150-170 proteins up-regulated by E2 exposure and ~200-220 proteins downregulated by E2 exposure in exosomes of both KTaR-1 and KTaV-3 Kisspeptin neurons. Estrogen-regulated Kiss-1 exosomal proteins include several candidates involved in bone remodeling (pentraxin, osteonectin, osteoclast-stimulating factor-1) and neuronal synaptic plasticity and signaling (annexins, semaphorins, connexins). Current work is exploring the effects of exposure of purified exosomes on morphology and gene expression in immortalized GnRH neurons and osteoblasts. While further study is required, initial results suggest that exosomes may represent additional cellular communication pathways utilized by Kisspeptin neurons to elicit changes in brain and bone.

scholarly journals IN VITRO NITROGEN METABOLISM AND ORGANOGENESIS OF NICOTIANA TABACUM

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1121c-1121
Author(s):  
Victoria E. Rudolph ◽  
David W. Burger
Keyword(s):  
Solid Medium ◽  
N Uptake ◽  
Dry Weight ◽  
Inorganic N ◽  
Net Uptake ◽  
History Of ◽  
N Metabolism ◽  
The Media

The role of N metabolism in organogenesis and growth was studied using tobacco pith callus. Callus was cultured on a solid medium containing 10 μM (1.75 mg/l) IAA and 2 μM (0.43 mg/l) kinetin for 56 days. In the growth experiment, ratios of NH4+-N to NO3--N (0:60, 20:40, 30:30, 40:20 and 60:0 mM) were supplied by (NH4)2 SO4 and KNO3. Callus and media were analyzed for inorganic N. Callus supported by 30:30 and 40:20 media removed the highest amounts of NH4+-N and NO3--N from the media and exhibited organogenesis. Final dry weight was greatest in callus supported by the 30:30 medium. In the organogenesis experiment, the transfer history of the inoculum source affected N uptake, organogenesis and growth. Inorganic N was supplied by NH4NO3 and KNO3-. The net uptake of NH4+-N and NO3--N was lower in shoot-forming than in root-forming and non-organogenic callus subculture from 7-day-old stock cultures. The final pH of the medium supporting shoot-forming callus was lowest. Growth, on a dry weight basis, was lowest in shoot-forming callus. Callus subculture from 60-day-old stock cultures formed no shoots.

scholarly journals Role of extracellular calcium in in vitro uptake and intraphagocytic location of macrolides

1995 ◽  
Vol 39 (8) ◽  
pp. 1676-1682 ◽  
Author(s):  
E. M. Mtairag ◽  
H. Abdelghaffar ◽  
C. Douhet ◽  
M. T. Labro
Keyword(s):  
Extracellular Calcium ◽  
In Vitro Uptake

The Role of Heparin on the Inhibition of Platelet Adhesion to Damaged Arterial Endothelial Surface

1975 ◽  
Author(s):  
E. M. Essien ◽  
R. Kinlough-Rathbone ◽  
S. Moore ◽  
J. Fraser Mustard
Keyword(s):  
Thoracic Aorta ◽  
Platelet Adhesion ◽  
Normal Pressure ◽  
In Vitro System ◽  
Endothelial Surface ◽  
Therapeutic Concepts ◽  
Rabbit Platelets ◽  
Adhesion Of Platelets

A technique for the isolation of rabbit thoracic aorta in a form in which it could withstand normal pressure in an in vitro system was developed. Selected segments of the aortae from different animals were damaged mechanically. These were then flushed through rapidly with a suitable dilution of heparin from hog mucosa and the excess heparin was washed off. When such treated aortae were exposed to rabbit platelets, adhesion of platelets to such surfaces compared to non-heparinised ones was significantly depressed (P < 0.001). A similar effect was demonstrable on undamaged aortic surfaces (P < 0.006).It is suggested that heparin may interact directly and rapidly with damaged vascular surfaces and inhibit platelet adhesion to them. The significance of these results with particular reference to therapeutic concepts is discussed.

Diffusional support of arteries

1985 ◽  
Vol 248 (6) ◽  
pp. H901-H906 ◽  
Author(s):  
A. H. Werber ◽  
D. D. Heistad
Keyword(s):  
Thoracic Aorta ◽  
Abdominal Aorta ◽  
Nutritional Support ◽  
Vasa Vasorum ◽  
Relative Role ◽  
Arterial Lumen ◽  
Aortic Media ◽  
The Media ◽  
Thoracic And Abdominal

The relative role of the arterial lumen, adventitial vasa vasorum, and medial vasa vasorum in nutritional support of arteries is unclear. We have used a newly developed autoradiographic method to study diffusion of metabolically inert [14C]antipyrine into arteries to determine the relative importance of different pathways in nutritional support of arteries. [14C]antipyrine was homogenously distributed across the media of small arteries within 15 s, which indicates that diffusion into the central media was rapid. In the thoracic and abdominal aorta, levels of antipyrine were higher in the inner media (P less than 0.05) than in the middle of the media. Levels of antipyrine in outer media of the thoracic aorta (which has medial and adventitial vasa) were comparable to those observed in the inner media, but antipyrine levels were lower in outer than in inner media of the abdominal aorta (which has adventitial vasa only). Ligation of intercostal arteries, which are the source of medial vasa vasorum in the thoracic aorta, decreased diffusional support to the outer media of the thoracic aorta. We conclude that 1) diffusional support is more effective in thinner muscular arteries than in the aorta, 2) both luminal and abluminal sources of nutrition are important, particularly for the aorta, 3) vasa vasorum appear to be important for adequate diffusional support of the thoracic aortic media, and 4) medial vasa vasorum may be more effective than adventitial vasa vasorum in nourishing the aorta.

Effect of pressure and intimal damage on 131I-albumin and [14C]sucrose spaces in aorta

1987 ◽  
Vol 253 (6) ◽  
pp. H1530-H1539 ◽  
Author(s):  
A. Tedgui ◽  
M. J. Lever
Keyword(s):  
Thoracic Aorta ◽  
Distribution Volume ◽  
Fluid Flux ◽  
Effect Of Pressure ◽  
Interstitial Volume ◽  
The Media ◽  
Protein Space

To measure the distribution volume for sucrose and albumin in the media of the rabbit thoracic aorta, we studied the uptake of tracers in vitro. In most cases the tracers were applied to both luminal and adventitial surfaces at the same concentration. When transmural convection was prevented by pressurization of the arteries with air, there was a decrease in sucrose space (19% at 70 mmHg and 28% at 180 mmHg) and in albumin space (60% at 70 mmHg and 66% at 180 mmHg), compared with the respective spaces in relaxed arteries (0.42 for sucrose and 0.08 for albumin). Much smaller changes were found when intact vessels were pressurized with liquid (insignificant for sucrose, decreases of 24% at 70 mmHg and 14% at 180 mmHg for albumin). Removal of the endothelium, which increases transmural fluid flux, increased the space for sucrose (not significantly at 70 mmHg and 17% at 180 mmHg) and particularly for albumin (100% at 70 mmHg and 250% at 180 mmHg). We conclude that the interstitial volume and consequently the protein space within the media can be modified both by distending stresses applied to the vessel and by transmural fluid flux.

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