scholarly journals IN VITRO NITROGEN METABOLISM AND ORGANOGENESIS OF NICOTIANA TABACUM

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1121c-1121
Author(s):  
Victoria E. Rudolph ◽  
David W. Burger

The role of N metabolism in organogenesis and growth was studied using tobacco pith callus. Callus was cultured on a solid medium containing 10 μM (1.75 mg/l) IAA and 2 μM (0.43 mg/l) kinetin for 56 days. In the growth experiment, ratios of NH4+-N to NO3--N (0:60, 20:40, 30:30, 40:20 and 60:0 mM) were supplied by (NH4)2 SO4 and KNO3. Callus and media were analyzed for inorganic N. Callus supported by 30:30 and 40:20 media removed the highest amounts of NH4+-N and NO3--N from the media and exhibited organogenesis. Final dry weight was greatest in callus supported by the 30:30 medium. In the organogenesis experiment, the transfer history of the inoculum source affected N uptake, organogenesis and growth. Inorganic N was supplied by NH4NO3 and KNO3-. The net uptake of NH4+-N and NO3--N was lower in shoot-forming than in root-forming and non-organogenic callus subculture from 7-day-old stock cultures. The final pH of the medium supporting shoot-forming callus was lowest. Growth, on a dry weight basis, was lowest in shoot-forming callus. Callus subculture from 60-day-old stock cultures formed no shoots.

2002 ◽  
Vol 138 (3) ◽  
pp. 255-260 ◽  
Author(s):  
S. J. GOKANI ◽  
V. S. THAKER

Fibres of three cotton cultivars (Gossypium hirsutum H-4, H-8 and G. arboreum G. Cot-15) were analysed for growth in terms of fibre length and dry weight and endogenous gibberellic acid (GA3) content thrice during 1997–2000, at Rajkot. The development of cotton fibre was divided into four distinct growth phases but overlap between elongation and secondary thickening was considerable which suggests that both these phases are independent of each other. During fibre elongation, GA3 content remained low and increased after a decrease in the rate of fibre elongation in all three genotypes. The long staple cultivar (H-4) showed highest endogenous GA3 content followed by the middle one (H-8) and the short staple cultivar (G. Cot-15). In in vitro studies when GA3, NAA or GA3+NAA was supplemented to the media, increase in fibre length of the short staple cultivar was maximum, followed by the middle one and the long staple cultivar. Both in vivo and in vitro findings suggest that GA3 is one of the important factors that determine fibre length.


1999 ◽  
Vol 5 (1-2) ◽  
Author(s):  
Gy. Végvári ◽  
I. Balla ◽  
Z. Kothencz

In vitro plant material of clones (Q. robur) NL 100 A (adult) and NL 100 R (rejuvenated) received from Germany (A. Meier-Dinkel, 1995) were used in these experiments. WPM medium was used for the multiplication phase. Plantlets were subcultured monthly. Differences in quality and colour of the adult and rejuvenated cultures induced us to follow and compare the changes of mineral- and chlorophyll content and dry weight during the propagation phase. Mineral and chlorophyll content as well as dry weight were measured weekly on three samples during the subculture period. In the case of propagation rates we stated, they were similar around the year, but both clones had a high peak in April. Examining the cation-content, we detected that, the plantlets had a highest quantity of several elements during the 2nd and 3rd week of subculture. The iron content was the highest in the 1st week and after that it decreased continuously. It is supposed, that the content of iron is not enough in the media. The chlorophyll content of the rejuvenated clone was higher than that of the adult one. In the rooting experiments it was stated that, after one-week cold treatment the rooting ability was the best.  


2013 ◽  
Vol 5 (1) ◽  
pp. 155-175 ◽  
Author(s):  
Hou Yuxin

Abstract The Wukan Incident attracted extensive attention both in China and around the world, and has been interpreted from many different perspectives. In both the media and academia, the focus has very much been on the temporal level of the Incident. The political and legal dimensions, as well as the implications of the Incident in terms of human rights have all been pored over. However, what all of these discussions have overlooked is the role played by religious force during the Incident. The village of Wukan has a history of over four hundred years, and is deeply influenced by the religious beliefs of its people. Within both the system of religious beliefs and in everyday life in the village, the divine immortal Zhenxiu Xianweng and the religious rite of casting shengbei have a powerful influence. In times of peace, Xianweng and casting shengbei work to bestow good fortune, wealth and longevity on both the village itself, and the individuals who live there. During the Wukan Incident, they had a harmonizing influence, and helped to unify and protect the people. Looking at the specific roles played by religion throughout the Wukan Incident will not only enable us to develop a more meaningful understanding of the cultural nature and the complexity of the Incident itself, it will also enrich our understanding, on a divine level, of innovations in social management.


Lumina ◽  
2020 ◽  
Vol 14 (1) ◽  
pp. 84-96
Author(s):  
Svetlana Simakova

The goal of the present study is to demonstrate the media-aesthetic potential of infographic messages on particular cases. This can be done due to an integrated approach to the analysis of the visual content of media content. That indicates the case study method implementation as well as description and generalization. The theoretical basis of the research is represented by scientific studies of various directions. That includes the history of media and visual media culture; features of the concepts of media culture and media language, media aesthetics; infographics as a tool of media language. The empirical basis of the study is journalistic materials containing infographic content of such publications as by RIA Novosti (ria.ru), TASS (tass.ru). The examples of visual image implementation in the transmission of information — media content containing infographics — are given and analyzed. Considering media aesthetics as the formation of a sensory perception of the proposed media content, the author turns to the philosophical and aesthetic foundations of visual practices in the media and post-humanistic trends in journalism. As a result of the analysis of the theoretical and practical basis of the research, the author comes to the conclusion that today the role of the media aesthetic component of messages is most relevant. And infographics, as the connecting link of language and consciousness, is its most striking tool.


2019 ◽  
Vol 13 (1) ◽  
Author(s):  
Zakwan Khrait

Abstract Background Infertility continues to be an enigmatic and emerging problem. Although in vitro fertilization has proved to be revolutionary and immensely beneficial to many people, it is far from perfect, and many women experience recurrent in vitro fertilization failures. There can be a multitude of factors involved in recurrent in vitro fertilization failures. The aim of this report was to explore the role of hysteroscopy in determining potential causes of in vitro fertilization failure and how the relevant hysteroscopic findings can address the issue of infertility in terms of a subsequent successful in vitro fertilization. Case presentation A 37-year-old white Arab woman with a history of eight in vitro fertilization failures and one curettage performed for a blighted ovum presented to our hospital because of inability to conceive. Her past medical history was significant for hypothyroidism and positive factor V Leiden. She underwent hystero contrast sonography, which revealed a normal uterine cavity with irregular fillings in the right corner. To explore this further, hysteroscopy was performed, which showed dense adhesions in the right upper corner and first-degree adhesions in the lower half of the uterus. After undergoing adhesiolysis and a cycle of estradiol valerate and progesterone, the patient successfully conceived twins. Conclusions Hysteroscopy may play an important role before or in conjunction with assisted reproductive techniques to help infertile women and couples achieve their goals of pregnancy and live birth of a child.


1998 ◽  
Vol 89 (1) ◽  
pp. 21-33 ◽  
Author(s):  
Andrea Witcomb

Much of the discussion of the impact of electronic technologies on museums suggests that electronic technologies have been important in displacing the traditional metaphor of museums as mausoleums. This paper supports the move away from this metaphor, but suggests that this is not a recent phenomenon or entirely attributable to the impact of electronic technologies. The rhetorics currently associated with electronic technologies in museums can be better understood as part of a longer history of the relation between museums and the media. This is a history which points to the role of the media in producing a public sphere in which more democratic social relations are possible.


1977 ◽  
Vol 55 (1) ◽  
pp. 161-168 ◽  
Author(s):  
Graham R. Daborn

The life history of B. mackini was studied in a large argillotrophic lake during 1970–1972. Hatching began immediately after spring thaw and was terminated by rising salinity 10 days to 2 weeks later. Growth rates reached maxima of 1 mm/day at 4 weeks of age and then declined as mean size approached 22–23 mm at 7–8 weeks. Clutch sizes varied as a function of female length. Maximum biomass of 580 mg dry weight/m2 (2700 cal/m2) was reached in late May, of which 3.9% per day was consumed by B. gigas. Source of the energy is presumed to be a bacteria – organic matter complex associated with suspended particles. Life cycle details are compared with other fairy shrimp species and the role of B. mackini in the community is discussed.


2020 ◽  
Vol 116 (5) ◽  
pp. 121-128
Author(s):  
Elena V. Kharitonova ◽  

The article deals with the peculiarities of translating mentality through language in a transforming society. The article reveals the idea of a transitive society and the psychology of transitivity. It is shown that a transitive society influences social representations and values, determines attitudes and goals. Particular attention is paid to the interaction of language and mentality in the context of globalization, when there is an increase in changes in language, including in the Internet language. The language of the people is one of the main mechanisms for transmitting the mentality, through which a special national way of thinking is formed. Numerous studies have shown that the transitivity of society, accompanied by the influence of high technologies, informatization, and virtualization, has a transformative effect on the mentality as a whole. In the history of Russia, the fundamental transformations of society associated with the revolution of 1917 also determined changes in the language in the post-revolutionary period, which were manifested in the increase in the number of jargon, abbreviations of words, and the introduction of foreign borrowings into the language. In the works of scientists of those years, the peculiarities of the influence of foreign borrowings on people's consciousness and mentality in general were analyzed. V. M. Bekhterev, N. S. Trubetskoy, A. M. Selishchev, A. A. Potebnya and others paid attention to the study of these processes. Excessive changes in language can pose a threat to the national mentality due to their impact on traditional values, their destruction and the introduction of new values in a globalized world. The role of the media as a native speaker of a new language and as a tool for influencing mass consciousness is outlined. The most intensive manipulation of the masses through language influences increases in unstable, transitional periods of society development.


2021 ◽  
Vol 23 (1) ◽  
pp. 380
Author(s):  
Sebastian Morales-Pison ◽  
Valentina Carrasco ◽  
Cristian Gutiérrez-Vera ◽  
José Miguel Reyes ◽  
Patricio Gonzalez-Hormazabal ◽  
...  

MicroRNA-423 (miR-423) is highly expressed in breast cancer (BC). Previously, our group showed that the SNP rs6505162:C>A located in the pre-miR-423 was significantly associated with increased familial BC risk in patients with a strong family history of BC. Therefore, in this study, we evaluated the functional role of rs6505162 in mammary tumorigenesis in vitro to corroborate the association of this SNP with BC risk. We found that rs6505162:C>A upregulated expression of both mature miR-423 sequences (3p and 5p). Moreover, pre-miR-423-A enhanced proliferation, and promoted cisplatin resistance in BC cell lines. We also showed that pre-miR-423-A expression decreased cisplatin-induced apoptosis, and increased BC cell migration and invasion. We propose that the rs6505162-A allele promotes miR-423 overexpression, and that the rs6505162-A allele induces BC cell proliferation, viability, chemoresistance, migration, and invasion, and decreases cell apoptosis as a consequence. We suggest that rs6505162:C>A is a functional SNP site with potential utility as a marker for early diagnosis, prognosis, and treatment efficacy monitoring in BRCA1/2-negative BC patients, as well as a possible therapeutic target.


Kurios ◽  
2021 ◽  
Vol 7 (2) ◽  
Author(s):  
Branckly Egbert Picanussa

The effectiveness of learning of Christian Religious Education at school needs some media. Song, as a part of musical activity, is one of the media that is used by Christian Religious Education teachers to teach the Christian faith effectively. The aim of this article is to affirm the important role of the song as one of the learning media of Christian Religious Education at school. This Article is written in qualitative method with literature study approach and observation as its approach. This article explains the nature and the purpose of Christian Religious Education, music in church life, music in the history of thinking and practicing of Christian Religious Education, and the use of song as media in the learning of Christian Religious Education at school. 


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