Nutrient Availability for Lactuca sativa Cultivated in an Amended Peatland: An Ionic Exchange Study

Few conservation strategies have been applied to cultivated peatland. This field study over one growth cycle of Lactuca sativa examined the effect of plant-based, high-C/N-ratio amendments in a real farming situation on peatland. Plant Root Simulator (PRS®) probes were used directly in the field to assess the impacts of incorporating Miscanthus x giganteus straw and Salix miyabeana chips on nutrient availability for lettuce. The results showed that lettuce yield decreased by 35% in the miscanthus straw treatment and by 14% in the willow chip treatment. In addition, the nitrogen flux rate was severely reduced during crop growth (75% reduction) and the plant N uptake index was much lower in the amended treatments than in the control. The phosphorus supply rate was also significantly lower (24% reduction) in the willow treatment. The influence of sampling zone was significant as well, with most macro-nutrients being depleted in the root zone and most micro-nutrients being mobilized. Additional work is needed to optimize the proposed conservation strategy and investigate the effects of consecutive years of soil amendment on different vegetable crops and in different types of cultivated peatlands to confirm and generalize the findings of this study. Future field studies should also explore the long-term carbon dynamics under plant-based, high-C/N-ratio amendments to determine if they can offset annual C losses.

Assessing Crop and Water Productivity Performance of Different Crops and Cropping Systems

Background: Freshwater in sufficient quantity and adequate quality is a prerequisite for human societies and natural ecosystems. To adequately feed 9.3 billion people in 2050, consumptive water use (i.e. transpired water) by all food and fodder crops needs to increase from its present estimated level of 7000 km3/year to 12,586 km3/year. However, fresh water resources are increasingly getting scarce because of increased competition among a multitude of users. Getting agriculture to perform with progressively smaller allocation of renewable water resources will remain a challenge on global scale. To meet the challenge there is an urgent need to improve the crop water productivity to ensure the sustainability of agriculture. Methods: An experiment was carried out at village Menawali, Hanumangarh, Rajasthan during both kharif and rabi seasons to assess productivity, economics, N-uptake and water use of different crops. An area of 187 ha comprising 25 farmers irrigated by common irrigation channels were selected to collect the information. The information required i.e. soil, crop management, growth, phonological, yields, water balance, N-uptake and water use efficiency of each crops were collected from 15 farmers. Bt-cotton and clusterbean of kharif and wheat and Indian mustard in rabi were prominent crops, cotton-wheat, cotton-mustard, clusterbean-wheat and clusterbean-mustard were major cropping sequences of the study region. Result: In kharif season, Bt-cotton gave higher economic yields than clusterbean and amongst rabi season crops, economic yields of wheat and mustard were 4255, 1778 kg/ha, respectively. The economic yield of cropping sequences varied from 3741-6514 kg/ha and were higher for cotton-wheat (6218 kg/ha), intermediate for clusterbean-wheat (5785 kg/ha) and lower for cotton-mustard (3741 kg/ha) and clusterbean-mustard (3308 kg/ha). The cotton-wheat (₹1181.2 mm) sequence had highest water use. Clusterbean-wheat cropping system recorded highest water productivity (16.5 kg/ha mm) followed by clusterbean-mustard (14.9 kg/ha mm). The clusterbean-mustard (₹456/ha mm) cropping sequence was most profitable and fetched highest net return followed by clusterbean-wheat (₹383/ha mm).

Current Status and Future Prospective for Nitrogen Use Efficiency in Wheat (Triticum aestivum L.)

Although essential for achieving high crop yields required for the growing population worldwide, nitrogen, (N) in large amounts, along with its inefficient use, results in environmental pollution and increased greenhouse gas (GHG) emissions. Therefore, improved nitrogen use efficiency (NUE) has a significant role to play in the development of more sustainable crop production systems. Considering that wheat is one of the major crops cultivated in the world and contributes in high amounts to the large N footprint, designing sustainable wheat crop patterns, briefly defined by us in this review as the 3 Qs (high quantity, good quality and the quintessence of natural environment health) is urgently required. There are numerous indices used to benchmark N management for a specific crop, including wheat, but the misunderstanding of their specific functions could result in an under/overestimation of crop NUE. Thus, a better understanding of N dynamics in relation to wheat N cycling can enhance a higher efficiency of N use. In this sense, the aim of our review is to provide a critical analysis on the current knowledge with respect to wheat NUE. Further, considering the key traits involved in N uptake, assimilation, distribution and utilization efficiency, as well as genetics (G), environment (E) and management (M) interactions, we suggest a series of future perspectives that can enhance a better efficiency of N in wheat.

Enhanced Soybean Productivity by Inoculation With Indigenous Bradyrhizobium Strains in Agroecological Conditions of Northeast Germany

Commercial inoculants are often used to inoculate field-grown soybean in Europe. However, nodulation efficiencies in these areas are often low. To enhance biological nitrogen (N) fixation and increase domestic legume production, indigenous strains that are adapted to local conditions could be used to develop more effective inoculants. The objective of this study was to assess the ability of locally isolated Bradyrhizobium strains to enhance soybean productivity in different growing conditions of Northeast Germany. Three indigenous Bradyrhizobium isolates (GMF14, GMM36, and GEM96) were tested in combination with different soybean cultivars of different maturity groups and quality characteristics in one field trial and two greenhouse studies. The results showed a highly significant strain × cultivar interactions on nodulation response. Independent of the Bradyrhizobium strain, inoculated plants in the greenhouse showed higher nodulation, which corresponded with an increased N uptake than that in field conditions. There were significantly higher nodule numbers and nodule dry weights following GMF14 and GMM36 inoculation in well-watered soil, but only minor differences under drought conditions. Inoculation of the soybean cultivar Merlin with the strain GEM96 enhanced nodulation but did not correspond to an increased grain yield under field conditions. USDA110 was consistent in improving the grain yield of soybean cultivars Sultana and Siroca. On the other hand, GMM36 inoculation to Sultana and GEM96 inoculation to Siroca resulted in similar yields. Our results demonstrate that inoculation of locally adapted soybean cultivars with the indigenous isolates improves nodulation and yield attributes. Thus, to attain optimal symbiotic performance, the strains need to be matched with specific cultivars.

Ethephon Reduces Maize Nitrogen Uptake but Improves Nitrogen Utilization in Zea mays L.

Increasing use of plant density or/and nitrogen (N) application has been introduced to maize production in the past few decades. However, excessive planting density or/and use of fertilizer may cause reduced N use efficiency (NUE) and increased lodging risks. Ethephon application improves maize lodging resistance and has been an essential measure in maize intensive production systems associated with high plant density and N input in China. Limited information is available about the effect of ethephon on maize N use and the response to plant density under different N rates in the field. A three-year field study was conducted with two ethephon applications (0 and 90 g ha−1), four N application rates (0, 75, 150, and 225 kg N ha−1), and two plant densities (6.75 plants m−2 and 7.5 plants m−2) to evaluate the effects of ethephon on maize NUE indices (N agronomic efficiency, NAE; N recovery efficiency, NRE; N uptake efficiency, NUpE; N utilization efficiency, NUtE; partial factor productivity of N, PFPN), biomass, N concentration, grain yield and N uptake, and translocation properties. The results suggest that the application of ethephon decreased the grain yield by 1.83–5.74% due to the decrease of grain numbers and grain weight during the three experimental seasons. Meanwhile, lower biomass, NO3- and NH4+ fluxes in xylem bleeding sap, and total N uptake were observed under ethephon treatments. These resulted in lower NAE and NUpE under the ethephon treatment at a corresponding N application rate and plant density. The ethephon treatment had no significant effects on the N concentration in grains, and it decreased the N concentration in stover at the harvesting stage, while increasing the plant N concentration at the silking stage. Consequently, post-silking N remobilization was significantly increased by 14.10–32.64% under the ethephon treatment during the experimental periods. Meanwhile, NUtE significantly increased by ethephon.

Grass–Legume Mixtures Show Potential to Increase Above- and Belowground Biomass Production for Andean Forage-Based Fallows

Soils of the Andean highlands are under threat from cropping system intensification. Improved forage-based fallows offer great promise to address this issue, but research is needed to better understand the potential of species mixtures vs. monocultures to support multiple farmer objectives, especially forage production and soil conservation. We used a pot study to quantify above- and belowground biomass production as well as the total N uptake of grass–legume pairs between five grasses: (1) oat (Avena sativa), (2) ryegrass (Lolium multiflorum), (3) festulolium (Lolium × Festuca genera), (4) brome grass (Bromus catharticus), and (5) orchard grass (Dactylis glomerata), and four legumes: (1) vetch (Vicia dasycarpa), (2) red clover (Trifolium pratense), (3) black medic (Medicago lupulina), and (4) alfalfa (Medicago sativa) relative to the performance of each species in monoculture within two soils from the central Peruvian Andes. Grass–legume bicultures demonstrated significant overyielding, producing 65% and 28% more total dry biomass and total N uptake on average than monocultures. Aboveground biomass of bicultures was significantly influenced by the species of legume present, while belowground biomass was more affected by the grass species in the mixture. When evaluating the growth of each species separately, our findings indicate that overyielding was driven more by the enhanced growth of grasses relative to legumes. Our findings indicate that combining key functional groups (e.g., grass and legume, annual and perennial) offers great promise for developing improved fallows for supporting soil health and productivity in Andean agroecosystems.

Regulation of nitrogen balance and yield on greenhouse eggplant under biochar addition in Mollisol

Maintaining nitrogen (N) balance and inhibiting N leaching loss in the soil-crop system is crucial to maintaining yield and reducing the environmental pollution. This study investigated the effects of soil NO₃⁻-N content and accumulation, eggplant yield, N leaching and balance response to biochar addition, including regular fertilisation and irrigation (W + F), biochar addition with regular fertilisation and irrigation (W + F + B), and biochar addition with 20% fertilisation and irrigation reduction (0.8W + 0.8F + B) treatments. Compared with W + F, W + F + B and 0.8W + 0.8F + B increased soil NO₃⁻-N content in 0–40 cm and soil NO₃⁻-N accumulation in 0–20 cm, and raised harvest index, N surplus and balance. Simultaneously, 0.8W + 0.8F + B compared to W + F enhanced N use efficiency and N partial factor productivity, conversely, it decreased N dry matter production efficiency, N surplus and balance. Stepwise regression analysis demonstrated that the effect of NO₃⁻-N leaching lasted in 60 cm under biochar addition in the first year, and lasted in 20 cm without biochar application in the next year. Altogether, biochar addition with 20% fertilisation and irrigation reduction is the most suitable management strategy to decrease N surplus and leaching, and maintain eggplant N uptake in a two-year cycle system on greenhouse vegetables in Mollisols.

Transcriptional Landscape of Ectomycorrhizal Fungi and Their Host Provides Insight into N Uptake from Forest Soil

Although EMF are well known for their role in supporting tree N nutrition, the molecular mechanisms underlying N flux from the soil solution into the host through the ectomycorrhizal pathway remain widely unknown. Furthermore, ammonium and nitrate availability in the soil solution is subject to frequent oscillations that create a dynamic environment for the tree roots and associated microbes during N acquisition.

Optimization of Nitrogen Demand in Vegetables by Different Impacts on Autotrophic and Heterotrophic Nitrification

Aims The understanding of the interactions between N transformations and N uptake by plants in greenhouse soils with large N accumulation is still not clear. The aim is to understand the plant- soil interactions (vegetables) on N transformations with respect to N supply. Methods 15N tracing studies were conducted in two greenhouse soils to simultaneously quantify soil gross N transformation and plant N uptake rates using the Ntraceplant tool. Results There were significant feedbacks between vegetable N uptake and soil gross N transformation rates, whether soil N accumulation occurred or not. Plant NO3– uptake rates (UNO3) were higher than the NH4+ uptake rates (UNH4), which is consistent with the NO3–-preference of the vegetable plants studied. While UNH4 was still responsible for 6-49% of total N uptake rates, significantly negative relationships between UNH4 and NH4+ immobilization rate and autotrophic nitrification rate (ONH4) were observed. ONH4 was significantly inhibited in the presence of plants and decreased with time. ONH4 (1.11 mg N kg-1 d-1) was much lower than UNO3 (8.29 mg N kg-1 d-1) in the presence of plants. However, heterotrophic nitrification rate (ONrec), which ranged from 0.10 to 8.11 mg N kg-1 d-1 was significantly stimulated and was responsible for 5-97% of NO3– production in all plant treatments, providing additional NO3– to meet N requirements of plants and microorganisms.Conclusions The management of organic N fertilizers should be improved to stimulate inorganic N production via heterotrophic nitrification in greenhouse cultivation.