scholarly journals Pericytes Regulate Fibrinolytic Function of Brain Capillary Endothelial Cells

Stroke ◽  
2001 ◽  
Vol 32 (suppl_1) ◽  
pp. 359-359
Author(s):  
Jeong A Kim ◽  
Nam D Tran ◽  
Shur-Jen Wang ◽  
Mark Fisher
Keyword(s):  
Endothelial Cells ◽  
Human Brain ◽  
Plasminogen Activator ◽  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Regulatory Role ◽  
Brain Capillary ◽  
Brain Capillary Endothelial Cells ◽  
Brain Pericytes ◽  
Capillary Endothelial Cells

P110 Our prior work has shown that astrocytes inhibit fibrinolysis of brain capillary endothelial cells (eg, Stroke 1999:30;1671–1677). The complex cellular microenvironment at the blood-brain barrier includes pericytes, which are adjacent to and share basement membrane with brain capillary endothelial cells. We analyzed the hemostatic regulatory role of pericytes in a blood-brain barrier model consisting of human brain pericytes cultured on transwell membrane inserts with human brain capillary endothelial cells. We measured fibrinolysis proteins and function in media conditioned by 48 hour co-culture of human brain capillary endothelial cells and human brain pericytes, as well as brain capillary endothelial mono-cultures. Compared to endothelial mono-cultures, pericyte-endothelial co-cultures exhibited levels of tissue plasminogen activator (tPA) protein reduced by 50±15% (p<.05). Co-culture preparations showed 32±13% (p<.05) increase in levels of plasminogen activator inhibitor-1 (PAI-1) protein, the primary inhibitor of tPA. tPA activity of co-culture preparations was 54±17% (p<.05) of endothelial mono-culture preparations. These data demonstrate that human brain pericytes have an important hemostatic regulatory role for endothelial-dependent fibrinolysis in vitro. These findings provide further support for brain-specific hemostasis, with pericytes as well as astrocytes playing key regulatory roles.

Receptor-mediated transcytosis of transferrin through blood-brain barrier endothelial cells

1996 ◽  
Vol 270 (4) ◽  
pp. H1149-H1158 ◽  
Author(s):  
L. Descamps ◽  
M. P. Dehouck ◽  
G. Torpier ◽  
R. Cecchelli
Keyword(s):  
Endothelial Cells ◽  
Blood Brain Barrier ◽  
Bovine Brain ◽  
Brain Barrier ◽  
Double Labeling ◽  
Brain Capillary ◽  
Brain Capillary Endothelial Cells ◽  
Blood Brain ◽  
Capillary Endothelial Cells ◽  
A Cell

A cell culture model of the blood-brain barrier consisting of a coculture of bovine brain capillary endothelial cells (BBCECs) and astrocytes has been used to examine the mechanism of iron transport to the brain. Binding experiments showed that BBCECs express 35,000 high-affinity (concn at 50% receptor saturation = 11.3 +/- 2.1 nM) transferin (Tf) receptors per cell. In contrast to apo-transferrin (apoTf) we observed a specific transport of holo-transferrin (holoTf) across BBCECs. This transport was inhibited completely at low temperature. Moreover, the anti-Tf receptor antibody (OX-26) competitively inhibited holoTf uptake by BBCECs. Pulse-chase experiments demonstrated that only 10% of Tf was recycled to the luminal side of the cells, whereas the majority of Tf was transcytosed to the abluminal side; double-labeling experiments clearly demonstrated that iron crosses BBCECs bound to Tf. No intraendothelial degradation of Tf was observed, suggesting that the intraendothelial pathway through BBCECs bypasses the lysosomal compartment. These results clearly show that the iron-Tf complex is transcytosed across brain capillary endothelial cells by a receptor-mediated pathway without any degradation.

Upregulation of the Low Density Lipoprotein Receptor at the Blood-Brain Barrier: Intercommunications between Brain Capillary Endothelial Cells and Astrocytes

1987 ◽  
Vol 84 (1) ◽  
pp. 465-473
Author(s):  
Bénédicte Dehouck ◽  
Marie-Pierre Dehouck ◽  
Jean-Charles Fruchart ◽  
Roméo Cecchelli
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Blood Brain Barrier ◽  
Ldl Receptor ◽  
Receptor Expression ◽  
Brain Barrier ◽  
Brain Capillary ◽  
Ldl Receptors ◽  
Brain Capillary Endothelial Cells ◽  
Capillary Endothelial Cells

In contrast to the endothelial cells in large vessels where LDL receptors are downregulated, brain capillary endothelial cells in vivo express an LDL receptor. Using a cell culture model of the blood-brain barrier consisting of a coculture of brain capillary endothelial cells and astrocytes, we observed that the capacity of endothelial cells to bind LDL is enhanced threefold when cocultured with astrocytes. We next investigated the ability of astrocytes to modulate endothelial cell LDL receptor expression. We have shown that the lipid requirement of astrocytes increases the expression of endothelial cell LDL receptors. Experiments with dialysis membranes of different pore size showed that this effect is mediated by a soluble factor(s) with relative molecular mass somewhere between 3,500 and 14,000. Substituting astrocytes with smooth muscle cells or brain endothelium with endothelium from the aorta or the adrenal cortex did not enhance the luminal LDL receptor expression on endothelial cells, demonstrating the specificity of the interactions. This factor(s) is exclusively secreted by astrocytes cocultured with brain capillary endothelial cells, but it also upregulates the LDL receptor on other cell types. This study confirms the notion that the final fine tuning of cell differentiation is under local control.

scholarly journals TNAP and EHD1 Are Over-Expressed in Bovine Brain Capillary Endothelial Cells after the Re-Induction of Blood-Brain Barrier Properties

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e48428 ◽  
Author(s):  
Barbara Deracinois ◽  
Sophie Duban-Deweer ◽  
Gwënaël Pottiez ◽  
Roméo Cecchelli ◽  
Yannis Karamanos ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Blood Brain Barrier ◽  
Barrier Properties ◽  
Bovine Brain ◽  
Brain Barrier ◽  
Brain Capillary ◽  
Brain Capillary Endothelial Cells ◽  
Blood Brain ◽  
Capillary Endothelial Cells

scholarly journals Physiological Pathway for Low-Density Lipoproteins across the Blood-Brain Barrier: Transcytosis through Brain Capillary Endothelial Cells In Vitro

Endothelium ◽  
2008 ◽  
Vol 15 (5-6) ◽  
pp. 254-264 ◽  
Author(s):  
Pietra Candela ◽  
Fabien Gosselet ◽  
Florence Miller ◽  
Valérie Buee-Scherrer ◽  
Gérard Torpier ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Blood Brain Barrier ◽  
Low Density Lipoproteins ◽  
Brain Barrier ◽  
Low Density ◽  
Brain Capillary ◽  
Brain Capillary Endothelial Cells ◽  
Blood Brain ◽  
Capillary Endothelial Cells

IL-25 expressed by brain capillary endothelial cells rescues blood-brain barrier dysfunction

2009 ◽  
Vol 65 ◽  
pp. S150
Author(s):  
Yoshifumi Sonobe ◽  
Hideyuki Takeuchi ◽  
Kunio Kataoka ◽  
Hua Li ◽  
Shijie Jin ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Barrier Dysfunction ◽  
Brain Capillary ◽  
Brain Capillary Endothelial Cells ◽  
Blood Brain ◽  
Capillary Endothelial Cells

scholarly journals Upregulation of the low density lipoprotein receptor at the blood-brain barrier: intercommunications between brain capillary endothelial cells and astrocytes.

1994 ◽  
Vol 126 (2) ◽  
pp. 465-473 ◽  
Author(s):  
B Dehouck ◽  
M P Dehouck ◽  
J C Fruchart ◽  
R Cecchelli
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Blood Brain Barrier ◽  
Ldl Receptor ◽  
Receptor Expression ◽  
Brain Barrier ◽  
Brain Capillary ◽  
Ldl Receptors ◽  
Brain Capillary Endothelial Cells ◽  
Capillary Endothelial Cells

In contrast to the endothelial cells in large vessels where LDL receptors are downregulated, brain capillary endothelial cells in vivo express an LDL receptor. Using a cell culture model of the blood-brain barrier consisting of a coculture of brain capillary endothelial cells and astrocytes, we observed that the capacity of endothelial cells to bind LDL is enhanced threefold when cocultured with astrocytes. We next investigated the ability of astrocytes to modulate endothelial cell LDL receptor expression. We have shown that the lipid requirement of astrocytes increases the expression of endothelial cell LDL receptors. Experiments with dialysis membranes of different pore size showed that this effect is mediated by a soluble factor(s) with relative molecular mass somewhere between 3,500 and 14,000. Substituting astrocytes with smooth muscle cells or brain endothelium with endothelium from the aorta or the adrenal cortex did not enhance the luminal LDL receptor expression on endothelial cells, demonstrating the specificity of the interactions. This factor(s) is exclusively secreted by astrocytes cocultured with brain capillary endothelial cells, but it also upregulates the LDL receptor on other cell types. This study confirms the notion that the final fine tuning of cell differentiation is under local control.

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