Bursaphelenchus paraburgeri sp. n. (Nematoda: Parasitaphelenchidae) in packaging wood from Malaysia

Nematology ◽  
2012 ◽  
Vol 14 (1) ◽  
pp. 39-50 ◽  
Author(s):  
Jiangling Wang ◽  
Jianfeng Gu
Keyword(s):  
New Species ◽  
Body Length ◽  
Excretory Pore ◽  
Total Body Length ◽  
Lateral View ◽  
Deciduous Trees ◽  
Lateral Field ◽  
Female Tail ◽  
Total Body

Bursaphelenchus paraburgeri sp. n., isolated in Ningbo, China, from packaging wood of deciduous trees imported from Malaysia is described. It is characterised by a slim body (a=32-37), lateral field with four lines, excretory pore located anterior to the metacorpus, vulva at 75% of total body length, presence of a thick vulval flap in lateral view, post-uterine branch about two-thirds of the vulva-anus distance long, female tail long and conical (c=15), tapering to a finely rounded or bluntly pointed terminus, spicules have only weakly developed rostrum and condylus, and cross-striped in their central part, three pairs of ventro-sublateral papillae (one pair precloacal, two pairs postcloacal just anterior to the bursal flap and adjacent to each other) and a single precloacal midventral papilla. The new species belongs to the africanus group of the genus Bursaphelenchus and is most similar to B. burgermeisteri and B. obeche, from which it can be distinguished, as well as from other Bursaphelenchus species, by morphology, ITS-RFLP patterns and sequencing results.

Bursaphelenchus koreanus sp. n. (Nematoda: Parasitaphelenchidae) in packaging wood from South Korea

Nematology ◽  
2013 ◽  
Vol 15 (5) ◽  
pp. 601-609 ◽  
Author(s):  
Jianfeng Gu ◽  
Jiangling Wang ◽  
Xianfeng Chen
Keyword(s):  
New Species ◽  
South Korea ◽  
Body Length ◽  
Excretory Pore ◽  
Total Body Length ◽  
Lateral View ◽  
Lateral Field ◽  
Female Tail ◽  
Total Body

Bursaphelenchus koreanus sp. n., isolated in Ningbo, P.R. China, from packaging wood made from Pinus sp. imported from South Korea, is described. It is characterised by a slim body (a = 28-35), lateral field with four lines, excretory pore located at level of, or slightly posterior to, median bulb, vulva at 75% of total body length, presence of a distinct vulval flap in lateral view, post-uterine branch extending for ca two-thirds of vulva-anus distance, female tail conical and ventrally bent with slightly pointed, irregular or roughened terminus (c′ = 4.1), spicules large and arcuate (27-34 μm) with pointed rostrum, cucullus visible but sometimes reduced to a small blunt extension. The new species belongs to the xylophilus group and is most similar to B. luxuriosae and B. paraluxuriosae. It is distinguished from other Bursaphelenchus species by morphology, ITS-RFLP patterns and sequencing results.

Bursaphelenchus paraluxuriosae sp. n. (Nematoda: Parasitaphelenchidae) in packaging wood from Indonesia

Nematology ◽  
2012 ◽  
Vol 14 (7) ◽  
pp. 787-798 ◽  
Author(s):  
Jianfeng Gu ◽  
Jiangling Wang ◽  
Helen Braasch ◽  
Wolfgang Burgermeister ◽  
Thomas Schröder
Keyword(s):  
New Species ◽  
Body Length ◽  
Excretory Pore ◽  
Total Body Length ◽  
Lateral View ◽  
Precloacal Papilla ◽  
Lateral Field ◽  
Female Tail ◽  
Total Body

Bursaphelenchus paraluxuriosae sp. n., isolated in Ningbo, China, from packaging wood made from Alphitonia sp. (Rhamnaceae) imported from Indonesia is described. It is characterised by a slim body (a = 29-39), lateral field with four lines, excretory pore located at level of median bulb or slightly posterior, vulva at 75% of total body length, presence of a distinct vulval flap in lateral view, post-uterine branch ca two-thirds of the vulva-anus distance, female tail conical and slightly ventrally bent with irregular or roughened terminus (c′ = 4.4), spicules large and arcuate, with pointed rostrum and a cucullus, which is typical for the xylophilus group, but is reduced to a small blunt extension, and three pairs of ventro-sublateral papillae (one pair precloacal, two pairs postcloacal just anterior to the bursal flap and adjacent to each other) and a small single precloacal papilla present. The new species belongs to the xylophilus group and is most similar to B. luxuriosae. It is distinguished from other Bursaphelenchus species by morphology, ITS-RFLP patterns and sequencing results.

Bursaphelenchus posterovulvus sp. n. (Nematoda: Parasitaphelenchidae) in packaging wood from Singapore

Nematology ◽  
2014 ◽  
Vol 16 (4) ◽  
pp. 403-410 ◽  
Author(s):  
Jianfeng Gu ◽  
Ning Wang ◽  
Jie He ◽  
Jiangling Wang ◽  
Xianfeng Chen ◽  
...  
Keyword(s):  
New Species ◽  
Body Length ◽  
Excretory Pore ◽  
Phylogenetic Analyses ◽  
Total Body Length ◽  
Precloacal Papilla ◽  
Lateral Field ◽  
Female Tail ◽  
Total Body

Bursaphelenchus posterovulvussp. n., isolated in Ningbo, P.R. China, from non-coniferous packaging wood from Singapore is described. It is characterised by a slim body (a = 24-43), lip region strongly offset, stylet with prominent and strongly sclerotised basal swellings, lateral field with four lines, excretory pore located at level of median bulb, hemizonid 30-40 μm posterior to excretory pore, vulva at 82-86% of total body length, post-uterine branch extending forcatwo-thirds of vulva-anus distance, female tail conical and short (c′ = 2.3-3.0) with a pointed terminus, spicules short (11-16 μm) and slightly arcuate, rostrum blunt and relatively short, dorsal and ventral limbs conspicuous and their distal ends separated, three pairs of ventro-sublateral papillae and a single precloacal papilla. The new species is distinguished from otherBursaphelenchusspecies by morphology, ITS-RFLP patterns and phylogenetic analyses.

Bursaphelenchus singaporensis sp. n. (Nematoda: Parasitaph-elenchidae) in packaging wood from Singapore—a new species of the B. xylophilus group

Zootaxa ◽  
2005 ◽  
Vol 988 (1) ◽  
pp. 1 ◽  
Author(s):  
JIANFENG GU ◽  
JIANCHENG ZHANG ◽  
HELEN BRAASCH ◽  
WOLFGANG BURGERMEISTER
Keyword(s):  
New Species ◽  
Body Length ◽  
Morphological Differentiation ◽  
Deciduous Trees ◽  
Lateral Field ◽  
Distal Extremity ◽  
Female Tail ◽  
Males And Females ◽  
Group B ◽  
A New Species

Bursaphelenchus singaporensis sp. n. isolated in China from packaging wood of deciduous trees, imported from Singapore is described and illustrated. This new species clearly belongs to the B. xylophilus group, having males with the typically shaped spicules with a cucullus at their distal extremity, the typical position and number of caudal papillae (three pairs and one single) and the anterior vulval lip of the females developed as a distinct flap. The new species is characterized by a body length of 792 (553–950) µm and 850 (690–961) µm of males and females, respectively, robust body (a= 34 and 31, resp.), 15–16 µm-long stylet, lateral field with four lines, long postuterine branch (averaging 102 µm) and a strongly conoid female tail (c= 20) with a finely rounded, only slightly ventrally-bent terminus, male with very strong spicules (41–48 µm long), distinct rostrum and small cucullus, and a dorso-ventral visible terminal bursa. Bursaphelenchus singaporensis sp. n. is closely related to other species of the B. xylophilus group (B. xylophilus, B. mucronatus, B. kolymensis, B. fraudulentus, B. conicaudatus, B. baujardi and B. luxuriosae) and similar to B. abruptus. The morphological differentiation is mainly based on the shape of the female tail. However, B. singaporensis sp. n. differs from all other species of the B. xylophilus group by larger spicules. The new species can be differentiated from B. abruptus, B. xylophilus, B. mucronatus, B. fraudulentus, B. conicaudatus and B. luxuriosae by means of ITS-RFLP patterns.

A new entomopathogenic nematode, Steinernema hebeiense sp. n. (Rhabditida: Steinernematidae), from North China

Nematology ◽  
2006 ◽  
Vol 8 (4) ◽  
pp. 563-574 ◽  
Author(s):  
Maurice Moens ◽  
Sergei Spiridonov ◽  
Aihua Yan ◽  
Shulong Chen ◽  
Xiuhua Li
Keyword(s):  
New Species ◽  
Body Length ◽  
Entomopathogenic Nematode ◽  
Tail Length ◽  
Total Body Length ◽  
Molecular Data ◽  
Body Region ◽  
Lsu Rdna ◽  
Lateral Field ◽  
Total Body

AbstractA new species of Steinernema (Rhabditida), S. hebeiense sp. n. was isolated from sandy soil, Hebei Province, China. Diagnostic characters of infective juveniles of S. hebeiense sp. n. include total body length of 658 (610-710) μm, distance from anterior end to excretory pore of 48 (43-51) μm, tail length of 66 (63-71) μm, and E% ratio of 72 (65-80). The lateral field has eight ridges at the mid-body region (two very thin submarginal ridges, poorly discernible under light microscope, but visible in SEM); and the anterior end is slightly offset and flattened. First generation males have a body length of 1177 (1036-1450) μm, spicule length = 57 (51-63) μm, and gubernaculum length = 46 (38-50) μm. The spicules are light brown in colour and slightly curved; the manubrium is longer than wide. A tail mucron is present in second generation males. These morphometrical and morphometric features classify S. hebeiense sp. n. within the ' feltiae-kraussei-oregonense ' group. Molecular data also classify the new species within this group. Within this group, the smallest differences between the sequences of S. hebeiense sp. n. is with S. weiseri – 73 bp in the ITS rDNA and 13 bp in the studied partial sequence of the D2D3 expansion segment of LSU rDNA.

Description of Aphelenchoides xui sp. n. (Nematoda: Aphelenchoididae) in packaging wood from South Africa

Nematology ◽  
2013 ◽  
Vol 15 (3) ◽  
pp. 279-289 ◽  
Author(s):  
Xuan Wang ◽  
Pei Wang ◽  
Jianfeng Gu ◽  
Jiangling Wang ◽  
Hongmei Li
Keyword(s):  
South Africa ◽  
Phylogenetic Analysis ◽  
New Species ◽  
Body Length ◽  
Excretory Pore ◽  
Lateral Field ◽  
Male Tail ◽  
Female Tail ◽  
Group 2

Aphelenchoides xui sp. n. is described and illustrated from imported packaging wood from South Africa intercepted at Ningbo Port, China. The new species has a body length of 564-820 μm (males) and 549-882 μm (females). The cuticle is weakly annulated with four lines in the lateral field. The stylet is 11-13 μm long and has small basal swellings. The excretory pore is located ca one body diam. posterior to the median bulb, or 75-100 μm from the head. Spicules are relatively large (18-23 μm) with apex and rostrum rounded and well developed and the end of the dorsal limb clearly curved ventrad like a hook. The male tail bears six (2 + 2 + 2) caudal papillae. The spermatheca is axial and oblong and contains disc-like sperm. The female tail is conical, terminating in a complicated step-like projection, usually with many tiny nodular protuberances. The new species is morphologically similar to A. arcticus, A. haguei and A. parasaprophilus in Group 2 sensu Shahina, but is distinguished by spicule shape and form of the female tail terminus. Phylogenetic analysis based on SSU and partial LSU sequences revealed that A. xui sp. n. was closest to A. varicaudatus, although some species of Laimaphelenchus and Schistonchus were also included in the same branch.

Steinernema loci sp. n. and Steinernema thanhi sp. n. (Rhabditida: Steinernematidae) from Vietnam

Nematology ◽  
2001 ◽  
Vol 3 (6) ◽  
pp. 503-514 ◽  
Author(s):  
Maurice Moens ◽  
Ngoc Chau Nguyen ◽  
Ke Long Phan
Keyword(s):  
Body Length ◽  
First Generation ◽  
Tail Length ◽  
Total Body Length ◽  
Body Width ◽  
Lateral Field ◽  
Diagnostic Characters ◽  
Spicule Length ◽  
Third Stage ◽  
Total Body

AbstractTwo new species of Steinernema (Rhabditida), Steinernema loci sp. n. and S. thanhi sp. n., were isolated from beach soil in Thanhhoa and Hatinh provinces in the northern part of Vietnam. A combination of morphological, morphometric and rDNA-RFLP features indicated the distinctness of both species from other Steinernema spp. Diagnostic characters of Steinernema loci sp. n. include: total body length (896-1072 μm), distance from anterior end to excretory pore (EP = 71-86 μm), tail length (66-83 μm), lateral field in mid-body with eight ridges (nine distinct lines) and rounded, broad and smooth anterior end of the third stage infective juvenile; first generation males were characterised by body width, spicule length and the ratio spicule length : anal body width (SW). Diagnostic characters of Steinernema thanhi sp. n. third stage infective juveniles include: total body length (720-960 μm), EP (68-84 μm), tail length (52-72 μm), lateral field in mid-body with eight ridges (nine distinct lines) and rounded, broad and smooth anterior end. Spicule length, ratio SW and arrangement of genital papillae characterised the first generation males.

Nematodes of the order Dorylaimida from Romania. The genus Enchodelus Thorne, 1939. 3. Species with rounded tail and long odontostyle

Nematology ◽  
2010 ◽  
Vol 12 (4) ◽  
pp. 609-618 ◽  
Author(s):  
Marcel Ciobanu ◽  
Iuliana Popovici ◽  
Pablo Guerrero ◽  
Reyes Peña-Santiago
Keyword(s):  
Body Length ◽  
Additional Data ◽  
Cell Mass ◽  
Total Body Length ◽  
Genital System ◽  
Neck Length ◽  
Female Tail ◽  
Female Genital System ◽  
Total Body ◽  
Female Genital

Abstract Three species of the genus Enchodelus, one new and two known, from natural areas in Romania are studied. Enchodelus carpaticus sp. n. is distinguished by its body 1.59-1.87 mm long, lip region offset by a marked depression and 17-20 μm diam., odontostyle 39.5-47 μm long and 2.1-2.5 lip region diam. or 2.3-2.8% of total body length, odontophore 42-51 μm long and with distinct basal flanges, neck 336-388 μm long, pharyngeal expansion 136-167 μm long or 39-45% of total neck length, presence of dorsal cell mass near cardia, female genital system amphidelphic, uterus tripartite and 144-195 μm long or 2.2-2.9 times the corresponding body diam., pars refringens vaginae with two trapezoidal sclerotisations, V = 42-50, and female tail short and rounded (21-29 μm long, c = 55-87, c′ = 0.5-0.7). Additional data, including LM pictures, are presented for E. macrodorus and E. saxifragae.

Ditylenchus sarvarae sp. n. (Tylenchina: Anguinidae) from Iran

Zootaxa ◽  
2018 ◽  
Vol 4399 (2) ◽  
pp. 197 ◽  
Author(s):  
EBRAHIM SHOKOOHI ◽  
FAHIME IRANPOUR ◽  
VLADA PENEVA ◽  
MILKA ELSHISHKA ◽  
HENDRIKA FOURIE ◽  
...  
Keyword(s):  
New Species ◽  
Body Length ◽  
Molecular Study ◽  
28S Rdna ◽  
The Other ◽  
Separate Group ◽  
Soil Nematodes ◽  
Lateral Field ◽  
Female Tail ◽  
A New Species

During a survey of soil nematodes in Iran, a new species of Ditylenchus Filipjev, 1936 was discovered. Ditylenchus sarvarae sp. n. is characterised by its body length (1.0–1.4 mm), lateral field with seven incisures and without areolation, long postuterine sac (1.8–2.8 times the corresponding body diameter) and conical female tail with pointed tip (68–89 μm long, c = 13.7–18.2, c' = 4.2–5.1). Male specimens have conical tails (64–70 μm long, c = 14.5–16.7, c' = 4.0–4.5), spicules (22–26 μm long) and gubernaculum (8–10 μm long). Measurements and illustrations are provided for this new species. A molecular study of the 28S rDNA region of D. sarvarae sp. n. demonstrates that the Iranian species belongs to a separate group compared with the other molecularly characterized species of genus Ditylenchus. 

Bursaphelenchus pterocarpi n. sp. (Tylenchina: Aphelenchoididae) found in Pterocarpus sp. imported into China from Ghana

Nematology ◽  
2019 ◽  
Vol 21 (7) ◽  
pp. 725-737
Author(s):  
Jianfeng Gu ◽  
Yiwu Fang ◽  
Lele Liu ◽  
Majid Pedram ◽  
Hongmei Li
Keyword(s):  
New Species ◽  
Body Length ◽  
Excretory Pore ◽  
Reproductive System ◽  
Female Body ◽  
Nerve Ring ◽  
Wild Type ◽  
Lateral Field ◽  
Group Species ◽  
Partial 18S

Summary Bursaphelenchus pterocarpi n. sp. isolated from Pterocarpus sp. logs imported from Ghana to Ningbo, China, is described and illustrated. The new species is characterised by a female body length of 630-946 μm, lateral field with four lines, a short 12.6-13.9 μm long stylet with small basal swellings, excretory pore located posterior to nerve ring, outstretched reproductive system with amoeboid sperm inside the spermatheca, a well-developed vulval flap, tail conical and straight with a 1.9-4.8 μm long mucron at the tip, and lacking males in both the wild type and fungal-cultured populations. In a concatenated tree, reconstructed on the basis of the partial 18S, full length ITS and partial 28S rDNA D2-D3 sequences, the new species occupied a basal placement to the clade of xylophilus-group and africanus-group species.

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