Community of macroparasites of the Pacific barracuda Sphyraena ensis Jordan and Gilbert, 1882 (Perciformes, Sphyraenidae) from the north coast of Peru

This study aimed to report the community of macroparasites of the Pacific barracuda Sphyraena ensis Jordan and Gilbert, 1882 (Perciformes, Sphyraenidae) from the north coast of Peru. From September to October 2019, 138 specimens of S. ensis were acquired from Caleta de Zorritos, Contralmirante Villar Province, Tumbes, on the north coast of Peru. The community of parasites found consisted of nine species of parasites including monogeneans, copepods, trematodes and nematodes. The total body length of the fish was negatively correlated with the mean intensity of infestation of Pseudochauhanea sp. The mean abundance of Pseudochauhanea sp. also showed a marked significant difference between the populations of male and female fish, being more associated with males. We provide a list of macroparasites recorded in fish of the genus Sphyraena in the Eastern Pacific Ocean. Checklist dataset published through GBIF (Doi: 10.15470/5htffh)

Community of macroparasites of the Pacific barracuda Sphyraena ensis Jordan and Gilbert, 1882 (Perciformes, Sphyraenidae) from the north coast of Peru

This study aimed to report the community of macroparasites of the Pacific barracuda Sphyraena ensis Jordan and Gilbert, 1882 (Perciformes, Sphyraenidae) from the north coast of Peru. From September to October 2019, 138 specimens of S. ensis were acquired from Caleta de Zorritos, Contralmirante Villar Province, Tumbes, on the north coast of Peru. The community of parasites found consisted of nine species of parasites including monogeneans, copepods, trematodes and nematodes. The total body length of the fish was negatively correlated with the mean intensity of infestation of Pseudochauhanea sp. The mean abundance of Pseudochauhanea sp. also showed a marked significant difference between the populations of male and female fish, being more associated with males. We provide a list of macroparasites recorded in fish of the genus Sphyraena in the Eastern Pacific Ocean. Checklist dataset published through GBIF (Doi: 10.15470/5htffh)

Sexual and developmental variations of ecto-parasitism in damselflies

The prevalence and intensity of parasitism can have different fitness costs between sexes, and across species and developmental stages. This variation could arise because of species specific sexual and developmental differences in body condition, immunity, and resistance. Theory predicts that the prevalence of parasitism will be greater in individuals with poor body condition and the intensity of parasitism will be greater in individuals with larger body size. These predictions have been tested and verified in vertebrates. In insects, however, contradictory evidence has been found in different taxa. Here, we tested these predictions on two species of Agriocnemis (Agriocnemis femina and Agriocnemis pygmaea) damselflies, which are parasitized by Arrenurus water mite ectoparasites. We measured body weight, total body length, abdomen area and thorax area of non-parasitized damselflies and found body condition varied between males and females, between immature females and mature females and between A. femina and A. pygmaea. Then, we calculated the parasite prevalence, i.e., the frequency of parasitism and intensity, i.e., the number of parasites per infected damselfly in eleven natural populations of both species. In line to our predictions, we observed greater prevalence in immature females than mature females but found no difference in parasite prevalence between males and females. Furthermore, we found that parasite load was higher in females than males and in immature females than mature females. Our result also showed that the frequency and intensity of parasitism varied between the two studied species, being higher in A. pygmaea than A. femina. Our study provides evidence that parasitism impacts sexes, developmental stages and species differentially and suggests that variation may occur due to sex, developmental stage, and species-specific resistance and tolerance mechanism.

Analisis Akustik Target Strength Penyu Hijau (Chelonia Mydas) Melalui Pengukuran Secara Terkontrol Pada Frekuensi 200 kHz

ABSTRACT Turtles are reptiles that live in the sea and are able to migrate over long distances along the Indian Ocean, Pacific Ocean and Southeast Asia. Currently the number of turtle populations in nature has greatly decreased. This population decline is caused by natural factors and human activities that endanger the population directly or indirectly. At this time the tagging set technique (ID tag code) is a method that is often used to detect the presence of turtles by attaching tags to female turtles who are landing to lay eggs on the beach or while in captivity or the discovery of the mother turtle by fishermen. One other method that can be used to detect the presence of turtles is to use the acoustic method. The purpose of this study was to analyze the characteristics of the acoustic backscattering energy of the green turtle (Chelonia mydes) and to analyze the relationship between target strength and total body length of the green turtle (Chelonia mydas). This research was conducted in the Tapak Paderi waters pond, Bengkulu City, in August-October 2020. The average target strength (TS) value for green turtles was -48.07 dB. Based on the regression equation, the value of determination (R2) obtained is 0.78. Where this value shows that the total length of the green turtle has an effect of 78% on the average target strength (TS) value, while for 22% it is caused by other factors such as body shape, environmental factors and other factors. Based on the ANOVA test, it can be concluded that there is a relationship between the total length and the value of the target strength (TS) on the green turtle or the value of the total length has an influence on the value of the target strength (TS) on the green turtle.

Gamma-Aminobutyric Acid (GABA) Promotes Growth in Zebrafish Larvae by Inducing IGF-1 Expression via GABAA and GABAB Receptors

Insulin-like growth factor-1 (IGF-1) primarily increases the release of gamma-aminobutyric acid (GABA) in neurons; moreover, it is responsible for the promotion of longitudinal growth in children and adolescents. Therefore, in this study, we investigated whether exogenous GABA supplementation activates IGF-mediated growth performance. Zebrafish larvae treated with GABA at three days post fertilization (dpf) showed a significant increase in the total body length from 6 to 12 dpf through upregulation of growth-stimulating genes, including IGF-1, growth hormone-1 (GH-1), growth hormone receptor-1 (GHR-1), and cholecystokinin A (CCKA). In particular, at 9 dpf, GABA increased total body length from 3.60 ± 0.02 to 3.79 ± 0.03, 3.89 ± 0.02, and 3.92 ± 0.04 mm at concentrations of 6.25, 12.5, and 25 mM, and the effect of GABA at 25 mM was comparable to 4 mM β-glycerophosphate (GP)-treated larvae (3.98 ± 0.02 mm). Additionally, the highest concentration of GABA (50 mM) -induced death in 50% zebrafish larvae at 12 dpf. GABA also enhanced IGF-1 expression and secretion in preosteoblast MC3T3-E1 cells, concomitant with high levels of the IGF-1 receptor gene (IGF-1R). In zebrafish larvae, the GABA-induced growth rate was remarkably decreased in the presence of an IGF-1R inhibitor, picropodophyllin (PPP), which indicates that GABA-induced IGF-1 enhances growth rate via IGF-1R. Furthermore, we investigated the effect of GABA receptors on growth performance along with IGF-1 activation. Inhibitors of GABAA and GABAB receptors, namely bicuculline and CGP 46381, respectively, considerably inhibited GABA-induced growth rate in zebrafish larvae accompanied by a marked decrease in the expression of growth-stimulating genes, including IGF-1, GH-1, GHR-1, and CCKA, but not with an inhibitor of GABAC receptor, TPMPA. Additionally, IGF-1 and IGF-1R expression was impaired in bicuculline and CGP 46381-treated MC3T3-E1 cells, but not in the cells treated with TPMPA. Furthermore, treatment with bicuculline and CGP 46381 significantly downregulated GABA-induced IGF-1 release in MC3T3-E1 cells. These data indicate that GABA stimulates IGF-1 release via GABAA and GABAB receptors and leads to growth promotion performance via IGF-1R.

First report of an egg-predator nemertean worm in crabs from the south-eastern Pacific coast: Carcinonemertes camanchaco sp. nov

Nemertean worms belonging to the genus Carcinonemertes have been tied to the collapse of crab fisheries in the northeastern Pacific Ocean. A new species is described from egg masses of two commercial crabs, Cancer porteri and Romaleon setosum, inhabiting the central-north Chilean coast. This is the first species of Carcinonemertes described from the southeastern Pacific Ocean. Total body length of Carcinonemertes camanchaco sp. nov. ranged from 2.38 to 4.93 and from 4.29 to 8.92 mm, in males and females, respectively. Among others, traits that distinguish this new species from other previously described congeneric species include: presence of two gonad rows on each side of the intestine, a simple (not decorated) mucus sheath, and a relatively wide stylet basis. Maximum likelihood and Bayesian inference phylogenetic analyses distinguished this new species from all other species of Carcinonemertes with available cox1 sequences in GenBank. Prevalence and mean (± SD) intensity of C. camanchaco sp. nov. was 24% and 2.6 (± 2.07) worms per egg mass in C. porteri and 38.1% and 3.8 (± 2.4) worms per egg mass in R. setosum. The formal description of this new species represents the first step towards the understanding of this worm's impact on the health of crab fisheries in the southeastern Pacific Ocean.

Practical guide for microscopic identification of infectious gastrointestinal nematode larvae in sheep from Sardinia, Italy, backed by molecular analysis

Background Gastrointestinal nematodes (GIN) are ubiquitous in small ruminant farming, representing a major health and production concern. Given their differences in pathogenicity and the current problems regarding anthelmintic resistance, specific diagnosis of GIN is of significant importance. At present, the most widely applied method for this entails culture and microscopic analysis of third-stage larvae, allowing for identification at least to the genus level. Overall, a variety of keys for microscopic analysis have been published, showing substantial variation. Given this fact, this study aimed to produce a practical and updated guide for the identification of infective ovine GIN larvae. Methods Using existing keys and protocols, a total of 173larvae of the most common species/genera of ovine GIN from pooled faecal samples from Sardinia (Italy) were identified and extracted, and further individual molecular identification was performed. Morphometric and morphological data as well as high-quality photographs were collected and combined to produce the final guide. Results GIN microscopically and molecularly identified during this research include Trichostrongylus spp., Teladorsagia circumcincta, Haemonchus contortus, Cooperia curticei, and Chabertia ovina. Based on microscopic analysis, 73.5% of the larvae were correctly identified. Based on sheathed tail length, 91.8% were correctly classified into their respective preliminary groups. Conclusions It is crucial for the microscopic identification of infectious GIN larvae to examine each larva in its entirety and thus to take multiple characteristics into account to obtain an accurate diagnosis. However, a preliminary classification based on sheathed tail length (resulting in three groups: A, short; B, medium; C, long) was found to be effective. Further identification within group A can be achieved based on the presence of a cranial inflexion, caudal tubercles and full body measurements (Trichostrongylus spp. < 720 µm, T. circumcincta ≥ 720 µm). Larvae within group B can be differentiated based on sheathed tail morphometry (H. contortus > 65 µm, C. curticei ≤ 65 µm), the presence of cranial refractile bodies, total body length measurements (H. contortus ≤ 790 µm, C. curticei > 790 µm) and shape of the cranial extremity. Finally, all characteristics proposed for the differentiation between Oesophagostomum spp. and C. ovina larvae (group C) were found to have considerable restrictions. Graphical abstract

Catostylus tagi (Class: Scyphozoa, Order: Discomedusae, Suborder: Rhizostomida, Family: Catostylidae) life cycle and first insight into its ecology

Jellyfish proliferations, which are conspicuous and natural events, cause blooms that may lead to severe consequences for anthropogenic activities and ecosystem structure and functioning. Although research during the last decade has focused on factors influencing the different jellyfish life stages, few species currently have their full life cycle known. In this context, we describe for the first time the developmental stages in the life cycle of Catostylus tagi, from planula to young medusa, reared in the laboratory. The species displays the typical Rhizostomida metagenetic life cycle. Mature scyphistomae display 16 tentacles and a total body length of 1.5 ± 0.2 mm. Only podocyst production and strobilation were observed. Strobilation, occurring continuously under laboratory conditions, was mainly polydisc. The eight-rayed typical ephyrae, with a total body diameter of 2.4 ± 0.4 mm at detachment, showed development typical of the Rhizostomida. As a first step in studying this species’ ecology, we also present preliminary assessments of: (i) the influence of different temperature and salinity regimes on planulae survival, settlement and metamorphosis and (ii) the effect of temperature and diet on asexual reproduction. The results showed a high tolerance of planulae to a wide range of salinities (15‰ to 25‰), while polyp development was significantly faster at higher temperature (20–25 °C). Strobilation onset was 2–3 times faster at 20 °C (10.6 ± 5.4 to 15 ± 6.6 day at various tested diet) than at 15 °C (32.2 ± 3 day). Feeding was a key factor as unfed polyps never underwent strobilation during the trial. Finally, we present the spatial and seasonal distribution of C. tagi in the Tagus estuary (Portugal) in 2019, showing its occurrence throughout the year (except in April), with most observations recorded on the northern shoreline. As C. tagi shows the ability to form blooms and a wide tolerance for temperature and salinity (for planulae and medusae stage), it is essential to understand its life cycle.

Effects of stocking density on growth and skin quality of grower Nile crocodiles (Crocodylus niloticus)

Intensive Nile crocodile (Crocodylus niloticus) farming operates with considerable variation in housing and stocking density. In this study, current commercial stocking densities for crocodilians were investigated using 261 grower-phase crocodiles (15 months old, average total body length 94.5 cm, and average weight 2.7 kg). Low (2.60 m2 per crocodile), medium (1.24 m2 per crocodile), and high (0.41 m2 per crocodile) stocking densities were tested. Growth, morphometric measures, Fulton’s condition scores and skin qualities were assessed over a six-month (May - November 2017) period. High stocking density had no adverse effects on the growth of grower Nile crocodiles. Crocodiles stocked at medium and high densities outperformed those that were stocked at low density in Fulton’s body condition scores, change in body condition from the start to the end of the trial, and feed conversion efficiencies. However, the high and, to a lesser extent, the medium stocking densities resulted in lower skin quality scores compared with those in the low-density treatment because of teeth marks from more aggressive behaviour. The results indicated that the medium pen density treatment is closer to the ideal than either the high or low stocking density groups. Stocking densities that provide 0.41 m2 per crocodile or less should be avoided because of lower skin quality scores, which weigh more heavily than growth and feed efficiency responses in the financial viability of commercial crocodile farming in typical South African production systems.

Use of otolith morphology and morphometry for species discrimination of megrims Lepidorhombus spp. in the Central Eastern Adriatic Sea

Sagittal otolith morphology and otolith shape of two megrim species, Lepidorhombus boscii and Lepidorhombus whiffiagonis, were compared using descriptive morphological characters and Wavelet shape coefficients. Differences in otolith shape were examined by linear discriminant analysis (LDA). The first discriminant axis explained 66.6% of the variation between the groups, and the second axis 28.5%. Otolith classification based on LDA showed that in 79.5% of cases an otolith can be correctly classified based only on its shape. Relationships between otolith morphometric parameters (length and width) and fish size (total length) were described by fitting simple linear regression models. For L. boscii the highest positive correlation was found between right otolith width and total body length (R2 = 0.82), and for L. whiffiagonis between left otolith length and total body length (R2 = 0.39).