Morphological and genetic characterization of a new Armadillidium species (Oniscidea, Armadillidiidae) from Tunisia

Crustaceana ◽  
2018 ◽  
Vol 91 (2) ◽  
pp. 169-183
Author(s):  
S. Hamaied ◽  
F. Charfi-Cheikhrouha ◽  
B. M. Lombardo

Armadillidium boukorninense n. sp., a new species collected in northeastern Tunisia, is described. This species can be easily recognised by the tegument structure, the morphology of the cephalon and the high differentiation of the male pereopod 7. Its geographical distribution is limited to the northeast of Tunisia, in the maquis and garrigues habitats of sub-humid and semi-arid areas. This new species is morphologically similar to A. decorum. However, the morphological comparison between these two species showed a divergence at the cephalon, hind margin of pereon-epimeron 1, pereopod VII and pleotelson. An allozyme analysis of ten enzymatic systems (ME, PHI, EST, MPI, ACPH, GOT, IDH, PEP, PGM, MDH) confirmed this morphological differentiation. Among the eighteen loci that were analysed, differentiation in six diagnostic (Mpi, Pgm, Me, Acph-2, Pep-3, Got-2) and two partially-diagnostic loci (Idh-1, Phi),were easily recognized. Furthermore, high values of Nei’s genetic distance (Nei, 1978) () and indicated a high genetic differentiation of these two species. When taking into account these two approaches, A. boukorninense n. sp. is morphologically and genetically different from A. decorum.

Parasitology ◽  
2009 ◽  
Vol 136 (8) ◽  
pp. 875-885 ◽  
Author(s):  
L. M. McINNES ◽  
A. GILLETT ◽  
U. M. RYAN ◽  
J. AUSTEN ◽  
R. S. F. CAMPBELL ◽  
...  

SUMMARYThe morphology and genetic characterization of a new species of trypanosome infecting koalas (Phascolarctos cinereus) are described. Morphological analysis of bloodstream forms and phylogenetic analysis at the 18S rDNA and gGAPDH loci demonstrated this trypanosome species to be genetically distinct and most similar to Trypanosoma bennetti, an avian trypanosome with a genetic distance of 0·9% at the 18S rDNA and 10·7% at the gGAPDH locus. The trypanosome was detected by 18S rDNA PCR in the blood samples of 26 out of 68 (38·2%) koalas studied. The aetiological role of trypanosomes in koala disease is currently poorly defined, although infection with these parasites has been associated with severe clinical signs in a number of koalas. Based on biological and genetic characterization data, this trypanosome species infecting koalas is proposed to be a new species Trypanosome irwini n. sp.


1990 ◽  
Vol 68 (6) ◽  
pp. 1124-1131 ◽  
Author(s):  
D. Paugy ◽  
J.-F. Guégan ◽  
J.-F. Agnèse

A new species of Labeo (Cuvier, 1817) is defined and described from the Upper Niger River and Upper Senegal (Baoulé) River basins. Although it is diagnosed as a new species, there is some overlap with two other sympatric species, L. coubie and L. senegalensis, in identifying characters. This overlap is sufficient to introduce the alternative possibility that the putative new species is a hybrid. Three separate techniques were used to test the two competing hypotheses: morphological/morphometric analysis, chromosomal/enzymological analysis, and comparative parasitology. These complementary studies were carried out simultaneously but essentially independently, to minimize any scientific bias during the investigation. It was established that the three species can be distinguished by a combination of mouth morphology and meristics (notably gill raker counts). Though all three have the same chromosome number (2N = 50), the new species can be identified allelically by four homozygous loci that are not present in L. coubie and L. senegalensis. This genetic result indicates that the new species is sexually isolated from the other two. Also, calculations of Nei's genetic distance produce an index which is shorter between L. coubie and L. senegalensis than between either of these two and the prospective new species. All three species can be further separated by their specific monogenean gill-parasite complement: L. coubie (five Dogielius spp., seven Dactylogyrus spp.); L. senegalensis (two Dogielius spp., five Dactylogyrus spp.); Labeo roseopunctatus n.sp (1 Dogielius sp., one Dactylogyrus sp.). As a result of these investigations, the idea of hybridism is rejected and a formal taxonomic description of Labeo roseopunctatus n.sp. is included in this paper.


Check List ◽  
2009 ◽  
Vol 6 (1) ◽  
pp. 039 ◽  
Author(s):  
Maura Rejane de Araújo Mendes ◽  
Antonio Alberto Jorge Farias Castro

The Caatinga biome is located in the semi-arid region of northeastern Brazil and covers about 37 % of Piauí state. The main objective of the present study was a characterization of the Caatinga flora of the farm of Morro do Baixio, in state of Piauí, Brazil (06°51’13” S; 41°28’15” W, at 400 to 540 m above sea level) in view of the fact that very few such surveys were conducted in the state. The flora of the farm was surveyed monthly, during a year, to gather herbs, epiphytes, parasites, sub shrubs, shrubs and trees. We encountered 136 species belonging to 46 families, including a new species of Bauhinia. The richest families were Caesalpiniaceae (15 spp.), Fabaceae (11 spp.), Bignoniaceae and Mimosaceae (both with nine spp.). We observed a higher frequency of typical species from sedimentary Caatinga. However, local conditions favor the appearance of species that occur in Carrasco and Cerrado.


Phytotaxa ◽  
2016 ◽  
Vol 257 (3) ◽  
pp. 280 ◽  
Author(s):  
Hao Zhou ◽  
Si-rong Yi ◽  
Qi Gao ◽  
Jie Huang ◽  
Yu-jing Wei

Aspidistra revoluta (Asparagaceae) is described and illustrated as a new species from limestone areas in southern Chongqing Municipality, China. The new species can be distinguished from the other Aspidistra species by its unique umbrella-like pistil with large revolute stigma lobes that bent downwards and touch the base of the perigone. A detailed morphological comparison among A. revoluta, A. nanchuanensis and A. carnosa is provided. The pollen grains of A. revoluta are subspherical and inaperturate, with verrucous exine. The chromosome number is 2n = 38, and the karyotype is formulated as 2n = 22m + 6sm + 10st. The average length of chromosome complement is 4.50 μm, and the karyotype asymmetry indexes A1 and A2 are respectively 0.37±0.03 and 0.49±0.01.


Parasitology ◽  
2004 ◽  
Vol 130 (1) ◽  
pp. 31-40 ◽  
Author(s):  
D. J. MORRIS ◽  
R. S. TERRY ◽  
K. B. FERGUSON ◽  
J. E. SMITH ◽  
A. ADAMS

The development of a new species, Bacillidium vesiculoformis n. sp. (Microspora, Mrazekiidae), is described from the freshwater oligochaete Nais simplex (Oligochaeta, Naididae). Initial stages of parasite development consist of a monokaryotic merogony within a haemocyte of the intestinal blood sinus. The resulting hypertrophied haemocyte is attached to the chloragocytes of the sinus by fine cytoplasmic extensions with the sinus around the cell becoming greatly enlarged. The meronts within the haemocyte form diplokaryotic sporonts that undergo sporogenesis directly within the cytoplasm of the host cell. The infected cell becomes packed with spores and developmental stages, causing it dramatically to increase in size, eventually rupturing the oligochaete and cell. Sporogony appears to be disporoblastic. Released spores were observed to have an adhesive quality. Transmission studies conducted with mature spores failed to transmit the parasite horizontally although vertical transmission was observed. Phylogenetic analysis of the parasite demonstrated that B. vesiculoformis clustered with microsporidian parasites of bryozoa and two other microsporidians, Janacekia debaiseuxi and an unidentified Bacillidium sp.


Mycotaxon ◽  
2010 ◽  
Vol 112 (1) ◽  
pp. 55-63 ◽  
Author(s):  
Felipe Wartchow ◽  
M. Auxiliadora Q. Cavalcanti

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