Heterodera circeae sp. n. and H. scutellariae sp. n. (Tylenchida: Heteroderidae) from Germany, with notes on the goettingiana group

Nematology ◽  
2004 ◽  
Vol 6 (3) ◽  
pp. 343-355 ◽  
Author(s):  
Sergei Subbotin ◽  
Dieter Sturhan
Keyword(s):  
New Species ◽  
Tail Length ◽  
Molecular Data ◽  
The Other ◽  
Phylogenetic Position ◽  
Lateral Field ◽  
Second Stage ◽  
Rdna Sequences ◽  
Dicotyledonous Plants ◽  
Stylet Length

Abstract Two species of cyst-forming nematodes belonging to the goettingiana group are described from woodland in Germany. Heterodera circeae sp. n., parasitising Circaea lutetiana (Onagraceae), most closely resembles H. carotae and H. cruciferae, from which it differs by larger cysts (430-720 μm) and longer fenestral length (27-54 μm) and rounded stylet knobs of the second-stage juveniles. Heterodera scutellariae sp. n., found on the labiate Scutellaria galericulata (Lamiaceae), is distinguished from the other species of this group by shorter body (358-437 μm) and tail (41-53 μm) of the second-stage juveniles. The ITS-rDNA sequences of the new species are very similar and differ from each other by only a few nucleotides. The phylogenetic position of the new species within the group is presented based on analyses of molecular data. A revised diagnosis of the goettingiana group is proposed. The species of the group have lemon-shaped, ambifenestrate and abullate (small bullae occasionally present) cysts with egg sac, long vulval slit (> 35 μm), thin vulval bridge, low semifenestrae (average fenestral length 30-45 μm), and weak underbridge. Second-stage juveniles are characterised by body length > 400 μm, tail length > 45 μ m, hyaline tail portion > 20 μm, stylet length > 20 μm, lateral field with four incisures and small phasmids. All known species of the goettingiana group exclusively parasitise dicotyledonous plants.

Soil and litter nematode diversity of Mount Hamiguitan, the Philippines, with description of Bicirronema hamiguitanense n. sp. (Rhabditida: Bicirronematidae)

Nematology ◽  
2015 ◽  
Vol 17 (3) ◽  
pp. 325-344 ◽  
Author(s):  
Xue Qing ◽  
Wim Bert ◽  
Hanne Steel ◽  
Joeseph Quisado ◽  
Irma Tandingan De Ley
Keyword(s):  
New Species ◽  
Molecular Data ◽  
The Philippines ◽  
Phylogenetic Position ◽  
Species Status ◽  
Diagnostic Features ◽  
Lateral Field ◽  
Nematode Diversity ◽  
Common Group

The nematode diversity in soil and litter was investigated on Mount Hamiguitan, the Philippines, along four eco-habitats from elevations of 75-1600 m a.s.l. A total of 155 and 467 nematodes were identified to 39 and 62 genera from litter and soil, respectively. The nematode assemblages and diversity did not show any relation to eco-habitat or elevation. Bacterivorous nematodes were the most common group (37.5%). Acrobeloides was most abundant from the soil and Aphelenchoides from the litter. Bicirronema hamiguitanense n. sp. is herein described based on morphology, morphometrics and molecular data. The new species has the following diagnostic features: a wide lateral field one-fifth of its body diam. with four incisures forming two ornamented ridges; gubernaculum with proximal thickening; spicules (35-38 μm) and gubernaculum (18-20 μm) longer than B. caledoniense; and with 37 molecular autapomorphies supporting its new species status. The phylogenetic position of the new species within Cephalobomorpha is discussed.

Morphological and molecular characterisation of Coslenchus persicus n. sp. (Nematoda: Tylenchidae) from Iran

Nematology ◽  
2020 ◽  
pp. 1-10
Author(s):  
Manouchehr Hosseinvand ◽  
Ali Eskandari ◽  
Reza Ghaderi
Keyword(s):  
New Species ◽  
Molecular Data ◽  
Ribosomal Genes ◽  
28S Rrna ◽  
Lateral Field ◽  
Rrna Tree ◽  
Basal Bulb ◽  
Stylet Length ◽  
North Western ◽  
A New Species

Summary A new species of Coslenchus from north-western Iran is illustrated and described using morphological, morphometric and molecular data. Coslenchus persicus n. sp. is characterised by a medium-sized body of 694-810 μm, often 18 (seldom 20-22) longitudinal ridges excluding lateral field, head with four distinct annuli, stylet 9.6-10.8 μm long, pharyngeal basal bulb large and cylindrical, spermatheca offset and ovate, without sperm, vulval flaps extending for two annuli, 5-7 μm in length, tail striated with finely to bluntly rounded tip, 110-153 μm long and without males. The new species comes close to C. assamensis, C. diversus, C. japonicus, C. leiocephalus, C. maritus, C. multigyrus, C. paramaritus, C. pastor, and C. polonicus, but can be distinguished from all by differences in body length, stylet length and shape of basal bulb, post-vulval uterine sac, tail and certain morphometric data. Molecular data are provided for the characterisation of the new species using ribosomal genes (18S and 28S rRNA). In the 18S rRNA tree, the new species was placed in a clade including one isolate of Coslenchus sp. from Iran. In the reconstructed 28S rRNA phylogenetic tree, C. persicus n. sp. clustered with other known Coslenchus species.

A new entomopathogenic nematode, Steinernema hebeiense sp. n. (Rhabditida: Steinernematidae), from North China

Nematology ◽  
2006 ◽  
Vol 8 (4) ◽  
pp. 563-574 ◽  
Author(s):  
Maurice Moens ◽  
Sergei Spiridonov ◽  
Aihua Yan ◽  
Shulong Chen ◽  
Xiuhua Li
Keyword(s):  
New Species ◽  
Body Length ◽  
Entomopathogenic Nematode ◽  
Tail Length ◽  
Total Body Length ◽  
Molecular Data ◽  
Body Region ◽  
Lsu Rdna ◽  
Lateral Field ◽  
Total Body

AbstractA new species of Steinernema (Rhabditida), S. hebeiense sp. n. was isolated from sandy soil, Hebei Province, China. Diagnostic characters of infective juveniles of S. hebeiense sp. n. include total body length of 658 (610-710) μm, distance from anterior end to excretory pore of 48 (43-51) μm, tail length of 66 (63-71) μm, and E% ratio of 72 (65-80). The lateral field has eight ridges at the mid-body region (two very thin submarginal ridges, poorly discernible under light microscope, but visible in SEM); and the anterior end is slightly offset and flattened. First generation males have a body length of 1177 (1036-1450) μm, spicule length = 57 (51-63) μm, and gubernaculum length = 46 (38-50) μm. The spicules are light brown in colour and slightly curved; the manubrium is longer than wide. A tail mucron is present in second generation males. These morphometrical and morphometric features classify S. hebeiense sp. n. within the ' feltiae-kraussei-oregonense ' group. Molecular data also classify the new species within this group. Within this group, the smallest differences between the sequences of S. hebeiense sp. n. is with S. weiseri – 73 bp in the ITS rDNA and 13 bp in the studied partial sequence of the D2D3 expansion segment of LSU rDNA.

Description of Malenchus sexlineatus n. sp., new records of three known species of Malenchus Andrássy, 1968 (Nematoda: Tylenchidae) and notes on amphidial aperture development

Nematology ◽  
2016 ◽  
Vol 18 (2) ◽  
pp. 155-174 ◽  
Author(s):  
Xue Qing ◽  
Alcides Sánchez-Monge ◽  
Toon Janssen ◽  
Marjolein Couvreur ◽  
Wim Bert
Keyword(s):  
New Species ◽  
Developmental Process ◽  
Molecular Data ◽  
The Philippines ◽  
Morphological Data ◽  
Phylogenetic Position ◽  
Lateral Field ◽  
Lateral Lines ◽  
Morphological And Molecular Data ◽  
Short Body

A new species, Malenchus sexlineatus n. sp., discovered from the Philippines, is described based on morphological and molecular data. The new species is unusual in the genus by having six lateral lines. Malenchus sexlineatus n. sp. is distinguished from M. williamsi, the only other species in the genus with six lateral lines (based on currently available SEM data), by a shorter body of 278 (270-288) vs 452 (425-495) μm, shorter stylet (7.0 (6.2-7.5) vs 11-12 μm), narrower annulations (0.8 (0.7-0.8) vs 1.2-1.6 μm), lateral field comprising one elevated ridge in LM vs six well-separated incisures (resembling the lateral lines in Cephalenchus) in LM, the presence of S-shaped vs straight amphidial apertures, and vulval flaps absent or only one annuli long vs distinct. By having an exceptionally short body, M. sexlineatus n. sp. comes close to M. parvus, M. bryanti and M. acarayensis. However, there are significant differences in the lateral lines, annuli width and most morphometric ratios. Three known species, namely M. exiguus, M. nanellus and M. pachycephalus, all being first records and first representative from China, are characterised by morphological data. The new species was placed in a robustly supported clade containing two other Malenchus spp. and M. exiguus. Interestingly, M. pressulus was placed in a separate, unresolved phylogenetic position. However, the phylogenetic position of these clades could not be resolved within Tylenchidae. The shapes of the amphidial aperture and fovea within Malenchus are also compared and its possible developmental process is illustrated and discussed.

A new cystoid nematode, Cryphodera japonicum n. sp. (Nematoda: Heteroderidae), from the rhizosphere of Japanese Podocarpus macrophyllus

Nematology ◽  
2020 ◽  
Vol 22 (8) ◽  
pp. 891-905
Author(s):  
Jianfeng Gu ◽  
Munawar Maria ◽  
Yiwu Fang ◽  
Honghong Wang ◽  
Kan Zhuo
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Tail Length ◽  
Coi Gene ◽  
Second Stage ◽  
Close Relationship ◽  
Podocarpus Macrophyllus ◽  
Stylet Length ◽  
A New Species ◽  
Partial 18S

Summary Cryphodera japonicum n. sp., detected at Ningbo port, China, from the rhizosphere of imported Japanese Podocarpus macrophyllus, is described. The new species is characterised by females with a globose body, protruding vulval lips, slightly concave anus-vulva profile, a stylet length of 37.2 (31.1-41.3) μm and a vulva-anus distance of 38.1 (34.7-44.1) μm. Males possess two lip annuli, a stylet length of 27 (22.5-30.6) μm long, three lines in lateral fields and a spicule 21.5 (19.1-23.0) μm long. Second-stage juveniles have bodies 543 (506-588) μm long, three lip annuli, a stylet 31.7 (29.2-34.5) μm long, three lines in the lateral fields, a conoid tail with a narrow rounded terminus, and a relatively long hyaline region occupying half of the tail length. Phylogenetic analyses based on the D2-D3 expansion segments of the 28S, ITS, partial 18S rRNA, and COI gene revealed the unique position of this species with other heteroderid nematodes, supporting its status as a new species of Cryphodera. The new species showed a close relationship with C. brinkmani.

Data on some members of the family Tylenchidae (Nematoda: Tylenchina) from Iran

Biologia ◽  
2015 ◽  
Vol 70 (10) ◽  
Author(s):  
Yousef Panahandeh ◽  
Ebrahim Pourjam ◽  
Farzad Aliramaji ◽  
Majid Pedram
Keyword(s):  
Body Length ◽  
Phylogenetic Analyses ◽  
Tail Length ◽  
Iranian Population ◽  
Lateral Field ◽  
Rdna Sequences ◽  
The Family ◽  
Molecular Phylogenetic ◽  
28S Rdna Sequences ◽  
Stylet Length

AbstractThree species belonging to the family Tylenchidae, Malenchus nanellus, M. undulatus and Tylenchus naranensis, are reported for the first time for Iran. These species are characterized based on morphological and morphometric data. The Iranian population of M. nanellus is characterized by its body length ranging from 410-485 μm, cuticle annuli 1.1- 1.5 μm wide at mid-body and lateral field with two crenate lines, starting at the mid-region of procorpus and ending near 1/3 of tail length. The population of M. undulatus is characterized by its 458-526 μm body length, cuticle coarsely annulated, annuli 1.8-2.4 μm wide at mid-body, lateral field with crenate incisures, beginning at about half of the stylet length, ending at middle of tail, head narrower than adjacent body, median bulb well developed with prominent valve plates and functional males in population. Iranian population of T. naranensis, is characterized by having a 631-774 μm body length, lateral field with four lines, outer lines crenate, a stylet length ranging from 10-11 μm and a tail of 102-131 μm long. Molecular phylogenetic analyses based on partial 28S rDNA sequences of T. naranensis revealed its close affinity with the genus Filenchus. Other Tylenchidae genera, such as Aglenchus (including the newly sequenced isolate from Iran) and Coslenchus were sister taxa and formed a well-supported clade. Malenchus exiguus, a previously reported species from Iran and sequenced in the present study, formed a monophyletic clade with other species of Malenchus and Lelenchus leptosoma.

Review of the genus Scutylenchus Jairajpuri, 1971 (Nematoda: Tylenchida), with description of Scutylenchus dongtingensis n. sp. from rhizosphere soil of grass in China

Zootaxa ◽  
2012 ◽  
Vol 3437 (1) ◽  
pp. 32 ◽  
Author(s):  
CHUN–LING XU ◽  
HUI XIE ◽  
CHUAN–BO ZHAO ◽  
SONG–BAI ZHANG ◽  
XIU–MIN SU
Keyword(s):  
New Species ◽  
Rhizosphere Soil ◽  
Ventral View ◽  
Tail Length ◽  
Lateral View ◽  
Hunan Province ◽  
Lateral Field ◽  
Female Tail ◽  
Stylet Length ◽  
A New Species

The genus Scutylenchus Jairajpuri 1971 (Nematoda: Tylenchida) is reviewed, and a compendium of the most importantdiagnostic characters with a key to the species are given. A new species, Scutylenchus dongtingensis n. sp., is describedfrom China, extracted from rhizosphere soil around grass near Dongting Lake in Hunan province. The new species ischaracterized by having body cuticle with 16–20 longitudinal striae forming blocks; lateral field with 6 incisures forming5 bands without areolation or any other decoration; a slightly offset head with 7 striae; slender stylet 22–25μm long, withcone about three fifths of stylet length; excretory pore inconspicuous under light microscope, located in region of anteriorpart of gland bulb in lateral view; female vulva slit transverse with small epiptygma in lateral view; conspicuous dot-likephasmid located at about one third of tail length posterior to anus; female tail subcylindrical, terminus round, annulated,terminal cuticle thickened; spicule slender, well developed, 27–32μm long, usually not protruded from cloaca;gubernaculum crescent-shaped, may be protruded from cloaca; bursa conspicuous, extending over tail terminus in ventral view.

Heterodera hainanensis n. sp. (Nematoda: Heteroderinae) from bamboo in Hainan Province, China – a new cyst nematode in the Afenestrata group

Nematology ◽  
2013 ◽  
Vol 15 (3) ◽  
pp. 303-314 ◽  
Author(s):  
Kan Zhuo ◽  
Honghong Wang ◽  
Weimin Ye ◽  
Deliang Peng ◽  
Jinling Liao
Keyword(s):  
Tail Length ◽  
Cyst Nematode ◽  
Phyllostachys Pubescens ◽  
Lateral Field ◽  
Second Stage ◽  
Slit Length ◽  
Stage Juvenile ◽  
Its Rrna ◽  
Stylet Length ◽  
Rrna Sequences

Heterodera hainanensis n. sp. is described and illustrated from bamboo (Phyllostachys pubescens) based on morphology and molecular analyses of rRNA D2D3 region of LSU and ITS. This new species belongs to the Afenestrata group and is characterised by lemon-shaped cyst with prominent vulval cone and vulval cone without fenestration and bullae but with weak and furcate underbridge. The second-stage juvenile (J2) has two lip annuli, a 15.8-17.5 μm long stylet with rounded knobs sloping slightly posteriorly, three incisures in the lateral field and a conical tail with hyaline portion forming 52.3-71.1% of the tail length. Heterodera hainanensis n. sp. most closely resembles species in the Afenestrata group but is distinguished from these species by a number of characters, including cyst shape and size, the presence of an underbridge, vulval slit length, J2 stylet length, stylet knobs shape, incisure number and lip annule number. Molecular analysis reveals that this species has unique LSU D2D3 and ITS rRNA sequences and RFLP-ITS-rRNA profiles, and it is most closest to H. koreana in dendrograms inferred from both markers. A key to all species of the Afenestrata group is presented.

Steinernema huense sp. n., a new entomopathogenic nematode (Nematoda: Steinernematidae) from Vietnam

Nematology ◽  
2014 ◽  
Vol 16 (7) ◽  
pp. 761-775 ◽  
Author(s):  
Ke Long Phan ◽  
Zdeněk Mráček ◽  
Vladimír Půža ◽  
Jiří Nermut ◽  
Andrea Jarošová
Keyword(s):  
New Species ◽  
National Park ◽  
First Generation ◽  
Entomopathogenic Nematode ◽  
Tail Length ◽  
Symbiotic Bacterium ◽  
Molecular Data ◽  
Lateral Field ◽  
Spicule Length ◽  
Short Body

A new entomopathogenic nematode, Steinernema huense sp. n., belonging to the carpocapsae group, was recovered in Bach Ma National Park (Thua Thien Hue province) Vietnam. Steinernema huense sp. n. infective juveniles are characterised by short body length of 527 (444-571) μm, distance from anterior end to excretory pore of 43 (38-46) μm, anterior end to end of pharynx of 116 (103-129) μm; tail length of 50 (43-56) μm, D% = 37 (34-39) and E% = 85 (79-93), H% = 44 (39-52) and in having six ridges (i.e., seven lines) in the lateral field. For first generation males, the diagnostic characters include the spicule length of 67 (60-72) μm; D% = 48 (41-61); SW% = 195 (168-238) and GS% = 76 (68-81). Both generations possess a minute filamentous mucron, which can be rudimentary in the first generation, and 23 genital papillae. Spicules are finely curved, brownish and slender with a prominent rostrum. First generation females have a non-protruding, symmetrical vulva, with double flapped epiptygma. The tail of mature females is obese with a short conical tip (peg) lacking minute protuberances, and post-anal swelling not developed. The new species is further characterised by sequences of ITS and D2-D3 regions of the ribosomal DNA. According to molecular data, the symbiotic bacterium of S. huense sp. n. is closely related to Xenorhabdus stockiae.

Steinernema yirgalemense n. sp. (Rhabditida: Steinernematidae) from Ethiopia

Nematology ◽  
2004 ◽  
Vol 6 (6) ◽  
pp. 839-856 ◽  
Author(s):  
Randy Gaugler ◽  
Mekete Tesfamariam ◽  
Byron Adams ◽  
Ugur Gozel ◽  
Khuong Nguyen
Keyword(s):  
New Species ◽  
Tail Length ◽  
Molecular Data ◽  
Pairwise Distance ◽  
Infective Juvenile ◽  
Sequence Length ◽  
Field Pattern ◽  
Lateral Field ◽  
Spicule Length ◽  
Steinernema Yirgalemense

AbstractSteinernema yirgalemense, a new species based on morphological and molecular data, is described from Yirgalem, Ethiopia. This nematode belongs to the 'bicornutum-group' which includes nematodes with horn-like structures in the labial region of the infective juvenile. The new species can be recognised by the infective juvenile having a body length of 635 (578–693) μm, distance from anterior and to excretory pore of 51 (45–59) μm; tail length of 62 (57–67) μm and E% (EP/tail length × 100) of 83 (67–90). The lateral field pattern is variable from anterior to posterior; the formula for the arrangement of ridges from head to tail being: 2, 6, 8, 6, 2. The new species can be further recognised by male characters: spicule with large velum and tapering posteriorly to a point, the ratio SW (spicule length/cloacal body diameter) and GS (gubernaculum length/spicule length) and especially by the presence of 12 pairs of genital papillae plus a single midventral papilla. The 12th pair, located at the edge of the cloacal aperture, is an important character. The presence of a low epiptygma is another diagnostic character of the new species. The new species is a sister taxon to S. abbasi and is characterised by the sequence length of the ITS regions (960 bp), ITS1 (270 bp), ITS2 (284 bp) and also by the pairwise distance of both ITS and D2/D3 regions between species of nematodes in the 'bicornutum-group'.

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