Paurodontella gilanica n. sp. (Nematoda: Sphaerularioidea) from Iran

Nematology ◽  
2018 ◽  
Vol 20 (5) ◽  
pp. 471-482 ◽  
Author(s):  
Ali Yaghoubi ◽  
Ebrahim Pourjam ◽  
Majid Pedram
Keyword(s):  
New Species ◽  
Posterior Probability ◽  
Small Body ◽  
Bootstrap Support ◽  
Bayesian Posterior Probability ◽  
Female Reproductive System ◽  
Lateral Field ◽  
Morphological Differences ◽  
Tail Tip ◽  
18S Rdna Sequence

Summary Paurodontella gilanica n. sp. is described and illustrated based on morphological, morphometric and molecular characters. The new species is characterised by its small body size, four lines in the lateral field, weak stylet with minute asymmetrical knobs, female reproductive system lacking a diverticulum but with small post-vulval uterine sac, elongate conoid tail with pointed, sometimes filiform, tip, males common with small spicules and cloacal bursa not reaching tail tip. Morphological differences between the new species and seven known species of the genus, namely P. asymmetrica, P. auriculata, P. balochistanica, P. minuta, P. myceliophaga, P. parapitica and P. persica, are discussed. The new species is also compared with four known species of Paurodontus having four lines in the lateral field. Using a 1123 nt long partial 18S rDNA sequence of the new species to reconstruct the phylogenetic relationships revealed that it formed a clade with members of the Sphaerulariidae and Paurodontidae. Using a 746 nt long partial sequence of the 28S rDNA D2-D3 segment revealed that P. gilanica n. sp. formed a clade with Abursanema iranicum in both Bayesian inference (BI) and maximum likelihood (ML) analyses with 0.99 Bayesian posterior probability (BPP) and 89% bootstrap support value (BS). The morphological affinities of Paurodontella and Paurodontus are discussed.

Description of Atetylenchus minor n. sp. (Tylenchina: Tylenchidae) and data on two other species of the family

Nematology ◽  
2015 ◽  
Vol 17 (8) ◽  
pp. 981-994 ◽  
Author(s):  
Ali Yaghoubi ◽  
Ebrahim Pourjam ◽  
Mohammad Reza Atighi ◽  
Majid Pedram
Keyword(s):  
New Species ◽  
Posterior Probability ◽  
Bayesian Posterior Probability ◽  
Lateral Field ◽  
Phylogenetic Studies ◽  
Sister Clade ◽  
The Family ◽  
Molecular Phylogenetic ◽  
Morphological Differences ◽  
Short Body

Three species of the family Tylenchidae, including one new and two known species, are described and illustrated. Atetylenchus minor n. sp. is characterised by its short body length of 633 (558-691) μm, MB = 45 (41-46), lateral field with four crenate incisures, stylet 12.0 (11.5-12.5) μm long, without distinct basal knobs but with swollen arms of the shaft at its base, V = 56.3 (53.7-59.3), absence of lateral vulval membranes, and conical tail 57 (51-62) μm long with a rounded tip. Morphological differences of the new species with four known species of the genus, namely A. abulbosus, A. graminus, A. amiri and A. metaporus, are discussed. Molecular phylogenetic studies of the new species using 1089 bp partial sequences of 18S rDNA revealed that the new species formed a clade with an unpublished species assigned to Psilenchus in Bayesian inference (BI) with low Bayesian posterior probability (BPP). This clade forms a sister clade to a major clade containing two species of Psilenchus and several belonolaim species and genera. The same result was achieved using maximum likelihood (ML) analysis. Morphological and molecular phylogenetic studies were also performed on two other species, Malenchus labiatus and Neothada cancellata, using 28S rDNA D2-D3 sequences. In the inferred Bayesian tree, M. labiatus formed a clade with other species of Malenchus with 28S information with maximal support (1.00 BPP), while N. cancellata formed a clade with Boleodorinae, again with maximal (1.00) BPP.

Description of a new species of the genus Anguillonema Fuchs, 1938 (Nematoda: Sphaerularioidea) with an identification key to the species

2018 ◽  
Vol 93 (04) ◽  
pp. 504-512
Author(s):  
A. Yaghoubi ◽  
E. Pourjam ◽  
M. Pedram
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Thin Body ◽  
Molecular Characteristics ◽  
Lateral Field ◽  
Phylogenetic Studies ◽  
Sister Clade ◽  
Molecular Phylogenetic ◽  
Morphological Differences ◽  
18S Rdna Sequence

AbstractAnguillonema iranicum n. sp. is described and illustrated as the second species of this genus from Iran, based on morphological, morphometric and molecular characteristics. It is identified by a short, thin body, a continuous lip region, six lines on the lateral field, a short, thin stylet, a posteriorly located pharyngo-intestinal junction to excretory pore, the presence of a post-vulval uterine sac, vulval position at 89% (87.4–89.9%) of body length, an elongate conoid tail with a rounded to pointed tip and not dorsally bent, and common functional males with short spicules and lacking a bursa. Morphological differences between the new species and the three known species of the genus, namely A. amolensis, A. crenati and A. poligraphi, are discussed. Molecular phylogenetic studies of the new species using partial 18S rDNA sequence revealed that it formed a sister clade with three species of Howardula, one species of Anguillonema and one unidentified isolate. In phylogenetic analyses using partial sequences of 28S rDNA D2-D3 segment, the new species formed a clade with two isolates of Parasitylenchus. A key to identification of Anguillonema species is also presented.

Bursaphelenchus antoniae sp. n. (Nematoda: Parasitaphelenchidae) associated with Hylobius sp. from Pinus pinaster in Portugal

Nematology ◽  
2006 ◽  
Vol 8 (5) ◽  
pp. 659-669 ◽  
Author(s):  
Ana Catarina Penas ◽  
Kai Metge ◽  
Manuel Mota ◽  
Vera Valadas
Keyword(s):  
New Species ◽  
Pinus Pinaster ◽  
Small Body ◽  
Petri Dish ◽  
The Body ◽  
Monilinia Fructicola ◽  
Precloacal Papilla ◽  
Lateral Field ◽  
Female Tail ◽  
18S Rdna Sequence

Abstract Bursaphelenchus antoniae sp. n. is described and illustrated. Dauer juveniles were isolated from the body of the large pine weevil, Hylobius sp., collected from maritime pine (Pinus pinaster) stumps, in Portugal. Bursaphelenchus antoniae sp. n. was reared and maintained in P. pinaster wood segments and on Petri dish cultures of the fungi Botrytis cinerea and Monilinia fructicola. The new species is characterised by a relatively small body length of ca 583 μ m (females) and 578 μ m (males), a lateral field with two incisures, presence of a small vulval flap and a conoid female tail with a rounded or pointed terminus. Males have stout spicules with a disc-like cucullus and seven caudal papillae arranged as a single midventral precloacal papilla, one precloacal pair and two postcloacal pairs. In the character of the lateral field, B. antoniae sp. n. comes close to B. abietinus, B. rainulfi and B. hylobianum, whilst spicule characters place it within the piniperdae-group sensu Ryss et al. Morphologically, B. antoniae sp. n. is closest to B. hylobianum; the spicules of these two species having flattened, wing-like, alae on the distal third of the lamina. Bursaphelenchus antoniae sp. n. is distinguished from B. hylobianum on the arrangement of the caudal papillae (two vs three pairs). ITS-RFLP profiles and the failure to hybridise support the separation of the two species. Phylogenetic analysis of the new species, based on the 18S rDNA sequence, supports the inclusion of this new species in the B. hylobianum-group sensu Braasch. Sequence analysis of the 28S rDNA D2/D3 domain did not place the new species in a definite group.

Description of two Pseudaphelenchus species (Tylenchomorpha: Aphelenchoididae) associated with Asian termites and proposal of Tylaphelenchinae n. subfam.

Nematology ◽  
2014 ◽  
Vol 16 (8) ◽  
pp. 963-978 ◽  
Author(s):  
Natsumi Kanzaki ◽  
Hou-Feng Li ◽  
Yen-Chiu Lan ◽  
Robin M. Giblin-Davis
Keyword(s):  
New Species ◽  
Small Body ◽  
Morphological Characters ◽  
Small Subunit ◽  
Coptotermes Formosanus ◽  
Phylogenetic Status ◽  
Two New Species ◽  
Molecular Phylogenetic ◽  
Morphological Differences ◽  
Tail Tip

Two new Pseudaphelenchus species were discovered from subterranean and arboreal termites from the East Asian subtropics. Pseudaphelenchus sui n. sp. isolated from Coptotermes formosanus was collected from Miyako Isl., Okinawa, Japan, and P. scheffrahni n. sp. was isolated from Nasutitermes takasagoensis collected from the Kenting National Park in Taiwan. The two new species and P. vindai, previously described from Panamanian termites, are close to each other, and are not easily distinguished morphologically, i.e., these three are almost ‘cryptic species’. However, they can be distinguished based on minor morphological differences, including shape of male bursa (clearest in P. vindai, vague in P. sui n. sp. and intermediate in P. scheffrahni n. sp.), male tail tip (P. sui n. sp. often have a small mucron but the others do not), and in the character of the female tail (the two new species have stronger ventral curvature than P. vindai, and further, P. sui n. sp. has clear annulation at distal part, while the other species do not). Molecular phylogenetic analysis based upon near-full-length sequences of the small subunit of the ribosomal RNA gene suggested that Pseudaphelenchus and Tylaphelenchus form a strongly-supported clade at the base of the family Aphelenchoididae, and that Tylaphelenchus is included in Pseudaphelenchus as an inner clade. Therefore, based on their phylogenetic status and common morphological characters, e.g., small body and spherical median bulb, a subfamily, Tylaphelenchinae n. subfam. is proposed to embrace these two genera. In addition, Ruehmaphelenchus ipidicola n. comb. (= Tylaphelenchus ipidicola) is proposed.

Description of Ruehmaphelenchus juliae n. sp. (Tylenchina: Aphelenchoididae) isolated from an ambrosia beetle, Xylosandrus crassiusculus (Motschulsky), from South Florida

Nematology ◽  
2015 ◽  
Vol 17 (6) ◽  
pp. 639-653 ◽  
Author(s):  
Natsumi Kanzaki ◽  
Robin M. Giblin-Davis ◽  
Rafael Gonzalez ◽  
Rita Duncan ◽  
Daniel Carrillo
Keyword(s):  
New Species ◽  
South Florida ◽  
Ambrosia Beetle ◽  
Internal Transcribed Spacer Region ◽  
Lateral Field ◽  
Mitochondrial Coi ◽  
Morphological Differences ◽  
Red Bay ◽  
Tail Tip ◽  
Southern Florida

During a survey of nematode associates of ambrosia beetles from dead and dying red bay and avocado trees affected by the laurel wilt epidemic in southern Florida, a Ruehmaphelenchus species was isolated from the non-native ambrosia beetle, Xylosandrus crassiusculus. The new species is characterised by its possession of an oral disc at the stoma opening, three lines in the lateral field, male spicule with clear dorsal and ventral limbs connected by elongated triangular cuticle, thin membrane-like tissue and cuticular bridge-like structure, conical tail with pointed tip of males and conical tail with digitate mucro of females. The new species is very similar to four previously described species: R. asiaticus, R. digitulus, R. thailandae and R. sirisus, and can be distinguished only by some minor morphological differences in male tail characters, i.e., spicule morphology, position of genital papillae and tail tip shape, and morphometric values. However, the new species is phylogenetically unique, i.e., it is the basal taxon of the Ruehmaphelenchus clade and close to Bursaphelenchus spp. Ruehmaphelenchus juliae n. sp. is therefore proposed based on its morphological diagnostic characters and molecular sequences of near-full-length of SSU, internal transcribed spacer region, D1, D2 and D3 expansion segments of LSU ribosomal RNA and partial mitochondrial COI genes.

Description of Ditylenchus acantholimonis n. sp. (Rhabditida: Anguinidae) from Iran, a morphological and molecular phylogenetic study

Nematology ◽  
2021 ◽  
pp. 1-10
Author(s):  
Samira Aliverdi ◽  
Ebrahim Pourjam ◽  
Majid Pedram
Keyword(s):  
New Species ◽  
Rhizosphere Soil ◽  
Phylogenetic Analyses ◽  
Phylogenetic Study ◽  
Pharyngeal Bulb ◽  
Lateral Field ◽  
Molecular Phylogenetic Study ◽  
Molecular Phylogenetic ◽  
Golestan Province ◽  
Tail Tip

Summary Ditylenchus acantholimonis n. sp. is described based on morphological, morphometric and molecular characters. It was isolated from the rhizosphere soil of Acantholimon sp. in Golestan province, Iran, and is mainly characterised by having four lines in the lateral field, a pyriform to bottle-shaped offset pharyngeal bulb, post-vulval uterine sac 36.6-56.1% of the vulva to anus distance long, and a subcylindrical to conical tail with widely rounded tip. It is further characterised by short to medium-sized females, 480-617 μm long, with a fine stylet having small rounded knobs, V = 80.8-83.6, c = 11.0-13.8, c′ = 3.3-4.6, and males with 16.0-17.0 μm long spicules. The new species was morphologically compared with six species having four lines in their lateral field, rounded tail tip and comparable morphometric data namely: D. dipsacoideus, D. emus, D. exilis, D. paraparvus, D. sturhani, and D. solani. It was also compared with two species, D. ferepolitor and D. angustus, forming a maximally supported clade in the 18S tree. The phylogenetic analyses using the maximal number of Anguinidae and several Sphaerularioidea genera based upon partial 18S and 28S rDNA D2-D3 sequences revealed that Ditylenchus is polyphyletic. In the 18S tree, the new species formed a clade with D. ferepolitor (KJ636374) and D. angustus (AJ966483); in the 28S tree it formed a poorly supported clade with D. phyllobios (KT192618) and Ditylenchus sp. (MG865719).

Bursaphelenchus rufipennis n. sp. (Nematoda: Parasitaphelenchinae) and redescription of Ektaphelenchus obtusus (Nematoda: Ektaphelenchinae), associates from nematangia on the hind wings of Dendroctonus rufipennis (Coleoptera: Scolytidae)

Nematology ◽  
2008 ◽  
Vol 10 (6) ◽  
pp. 925-955 ◽  
Author(s):  
Natsumi Kanzaki ◽  
Robin M. Giblin-Davis ◽  
Yasmin J. Cardoza ◽  
Weimin Ye ◽  
Kenneth F. Raffa ◽  
...  
Keyword(s):  
New Species ◽  
Morphological Characters ◽  
Morphological Examination ◽  
Dendroctonus Rufipennis ◽  
Lateral Field ◽  
The Past ◽  
Female Tail ◽  
Molecular Phylogenetic ◽  
Voucher Specimens ◽  
Tail Tip

Abstract Two species of aphelench, Bursaphelenchus rufipennis n. sp. and Ektaphelenchus obtusus, were isolated from the 'nematangia', cocoon-like structures found at the base of the hind wings of Dendroctonus rufipennis. The nematangia contained adult females of E. obtusus and the dauer juveniles of B. rufipennis n. sp. Only B. rufipennis n. sp. could be cultured on Monilinia fructicola on LGPDA (lactic acid-treated, glycerol-supplemented, potato dextrose agar). The new species of Bursaphelenchus is described and figured and some additional morphological characters are ascribed to E. obtusus, E. josephi, E. sandiaensis, E. smaelus (= E. prolobos) and E. terebranus after examination of type and/or voucher specimens. Bursaphelenchus rufipennis n. sp. has an adult body length of ca 500-1000 μm, medium a ratios (ca 25-38 for females and ca 30-40 for males), b ratios of ca 8-13 (female) and 7-11 (male), c ratios of ca 15-22 (female and male), c′ ratios of ca 3-4 (female) and ca 2-3 (male), and is characterised by three incisures in the lateral field, mitten-shaped spicules and a conical female tail that curves ventrally and possesses a variable tail tip. The new species is morphologically closest to B. corneolus, B. curvicaudatus, B. gerberae, B. paracorneolus and B. talonus. Morphological examination of type and/or voucher specimens of five Ektaphelenchus species revealed coarse transverse body annulation and three pairs of male caudal papillae (except for the two species where males are not described). Clear typological differences were observed among these five Ektaphelenchus species in the structure of the lip region, presence/absence of stylet knobs and male spicule morphology. Although these characters have not been consistently documented in the past, they may be diagnostic for species in the genus. Molecular phylogenetic analysis based on SSU and D2/D3 LSU sequences revealed that B. rufipennis n. sp. was closest to B. paracorneolus and that E. obtusus was closest to species of Ektaphelenchoides and a Cryptaphelenchus sp.

Steinernema ethiopiense sp. n. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Ethiopia

Nematology ◽  
2012 ◽  
Vol 14 (6) ◽  
pp. 741-757 ◽  
Author(s):  
Tewodros Tamiru ◽  
Tewodros Tamiru ◽  
Lieven Waeyenberge ◽  
Tewodros Tamiru ◽  
Lieven Waeyenberge ◽  
...  
Keyword(s):  
New Species ◽  
Phylogenetic Trees ◽  
First Generation ◽  
Second Generation ◽  
Dna Analysis ◽  
Galleria Mellonella ◽  
Entomopathogenic Nematode ◽  
Its Region ◽  
Lateral Field ◽  
Tail Tip

Three isolates (Dero-1, Dero-8 and Mosisa-1) of a new entomopathogenic nematode, S. ethiopiense sp. n., were isolated by baiting soil samples from the Mendi area, Western Wollega, Ethiopia, with last instar wax moth larvae Galleria mellonella. Infective juveniles of S. ethiopiense sp. n. have a body length of 898 (768-1010) μm, a maximum of eight identical ridges (i.e., nine lines) in the lateral field, excretory pore located at mid-pharynx, hyaline layer occupying approximately half of the tail and c′ = 3.2. First generation males lack a caudal mucron, whereas second generation males possess a short spine-like mucron. The spicules are slightly arcuate, golden-brown in colour and have an ellipsoid or oblong manubrium. First generation females lack a postanal swelling and have a minute protuberance on the tail tip whereas second generation females have a postanal swelling and protruding vulva. Based on the morphology, morphometrics and DNA analysis, the new species belongs to the glaseri group. The closest relative species is the afro-tropical S. karii recorded from Kenya. The BLAST analysis of the ITS region of the rDNA revealed a similarity of 93% with S. karii, supporting the validity of S. ethiopiense sp. n. as a new species. In the phylogenetic trees the new species groups together only with S. karii (bootstrap value of 100%), but is also separated from S. karii by a bootstrap value of 100% or 70%.

Bursaphelenchus eproctatus n. sp. (Nematoda: Parasitaphelenchidae) isolated from dead Japanese black pine, Pinus thunbergii Pars

Nematology ◽  
2008 ◽  
Vol 10 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Rina Sriwati ◽  
Natsumi Kanzaki ◽  
Long Ke Phan ◽  
Kazuyoshi Futai
Keyword(s):  
New Species ◽  
Dorsal Surface ◽  
Pinus Thunbergii ◽  
Medium Size ◽  
Black Pine ◽  
Lateral Field ◽  
Male Ratio ◽  
Female Tail ◽  
Japanese Black Pine ◽  
Tail Tip

Abstract Bursaphelenchus eproctatus n. sp. is described and figured from a dead Japanese black pine, Pinus thunbergii. The new species has a body length of 639 (580-714) μm in the female and 545 (550-595) μm in the male, ratio a = 35.9 (32.6-41.9) in the female and 33.8 (31.5-35.1) in the male, and c and c′ ratios = 14.2 (13.3-15.0) and 2.7 (2.3-3.0), respectively, in the male. A functional rectum and anus are absent, the intestine ending as a blind sac in the female posterior region. The female stylet = 18.8-21.8 μm long and the male stylet = 14.9-19.8 μm, the median bulb is elongate-oval and there are three lines in the lateral field. The spicules are of medium size, 19.8 (18.8-20.8) μm long, and arcuate with a terminal cucullus; there are two pairs of male caudal papillae and the female tail is tapered with a rounded terminus and a clearly annulated dorsal surface near the tail tip. Based upon spicule morphology the new species belongs to the B. piniperdae-group sensu Ryss and is close to B. hunanensis, B. minutus and B. lini. However, the new species is distinguished from these three species by a number of morphological features and morphometric values.

Morphological and molecular characterisation of Aphelenchoides primadentus n. sp. (Nematoda: Aphelenchoididae) from northern Iran

Nematology ◽  
2018 ◽  
Vol 20 (2) ◽  
pp. 97-109 ◽  
Author(s):  
Mahyar Mobasseri ◽  
Ebrahim Pourjam ◽  
Majid Pedram
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Small Subunit ◽  
Natural Forests ◽  
Basic Difference ◽  
Northern Iran ◽  
Lateral Field ◽  
Tail Tip ◽  
Genomic Regions ◽  
Species Being

Aphelenchoides primadentusn. sp. is described and illustrated based on its morphological, morphometric and molecular characters. It was recovered from natural forests of northern Iran in association with mosses. The new species is characterised by its body length of 502-613μm in females and 461-564μm in males, presence of three lines in the lateral fields, a low lip region slightly offset by a shallow depression, 11.1-13.8μm long stylet in females, its conus being longer than the shaft (m = 53.0-68.5) and bearing well-developed, rounded basal knobs, the excretory pore located atcaone body diam. posterior to the metacorpus, a conical tail with a multipapillate terminus or with a single, warty, wide mucron-like structure at the tip, and males with 19-22μm long arcuate spicules that lack a well-developed condylus and rostrum. The new species was morphologically compared with species of the genus having three lines in the lateral field and a multipapillate tail tip or tail with warty mucron, namelyA. iranicusandA. heidelbergi. Compared toA. ensete,A. huntensisandA. gorganensis, three species with a multipapillate tail tip or warty mucron at the tail tip, the new species has a basic difference in the number of lines in the lateral field and, compared to two species,A. composticolaandA. petersi, which have three lines in the lateral field and a similar overall morphology, the new species has basic differences in tail tip morphology. The results of phylogenetic analyses using partial sequences of ribosomal small subunit RNA (SSU) and mitochondrial cytochrome c oxidase 1 (COImtDNA) genes revealed there is currently no sequenced species of the genus phylogenetically close to the new species being sequenced for these two genomic and non-genomic regions. The new species forms a clade withA. ritzemabosiin SSU, and three other species,A. besseyi,A. ritzemabosiandA. fujianensis, in theCOItree.

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