Bursaphelenchus antoniae sp. n. (Nematoda: Parasitaphelenchidae) associated with Hylobius sp. from Pinus pinaster in Portugal

Nematology ◽  
2006 ◽  
Vol 8 (5) ◽  
pp. 659-669 ◽  
Author(s):  
Ana Catarina Penas ◽  
Kai Metge ◽  
Manuel Mota ◽  
Vera Valadas
Keyword(s):  
New Species ◽  
Pinus Pinaster ◽  
Small Body ◽  
Petri Dish ◽  
The Body ◽  
Monilinia Fructicola ◽  
Precloacal Papilla ◽  
Lateral Field ◽  
Female Tail ◽  
18S Rdna Sequence

Abstract Bursaphelenchus antoniae sp. n. is described and illustrated. Dauer juveniles were isolated from the body of the large pine weevil, Hylobius sp., collected from maritime pine (Pinus pinaster) stumps, in Portugal. Bursaphelenchus antoniae sp. n. was reared and maintained in P. pinaster wood segments and on Petri dish cultures of the fungi Botrytis cinerea and Monilinia fructicola. The new species is characterised by a relatively small body length of ca 583 μ m (females) and 578 μ m (males), a lateral field with two incisures, presence of a small vulval flap and a conoid female tail with a rounded or pointed terminus. Males have stout spicules with a disc-like cucullus and seven caudal papillae arranged as a single midventral precloacal papilla, one precloacal pair and two postcloacal pairs. In the character of the lateral field, B. antoniae sp. n. comes close to B. abietinus, B. rainulfi and B. hylobianum, whilst spicule characters place it within the piniperdae-group sensu Ryss et al. Morphologically, B. antoniae sp. n. is closest to B. hylobianum; the spicules of these two species having flattened, wing-like, alae on the distal third of the lamina. Bursaphelenchus antoniae sp. n. is distinguished from B. hylobianum on the arrangement of the caudal papillae (two vs three pairs). ITS-RFLP profiles and the failure to hybridise support the separation of the two species. Phylogenetic analysis of the new species, based on the 18S rDNA sequence, supports the inclusion of this new species in the B. hylobianum-group sensu Braasch. Sequence analysis of the 28S rDNA D2/D3 domain did not place the new species in a definite group.

Bursaphelenchus chengi sp. n. (Nematoda: Parasitaphelenchidae) isolated at Nanjing, China, in packaging wood from Taiwan

Nematology ◽  
2008 ◽  
Vol 10 (3) ◽  
pp. 335-346 ◽  
Author(s):  
Hongmei Li ◽  
Phap Quang Trinh ◽  
Lieven Waeyenberge ◽  
Maurice Moens
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Body Size ◽  
Posterior Margin ◽  
Petri Dish ◽  
Packaging Materials ◽  
Lateral Field ◽  
Female Tail ◽  
Domain Sequence ◽  
Lateral Lines

Abstract Bursaphelenchus chengi sp. n. is described and illustrated. Dauer juveniles were isolated from imported wood packaging materials from Taiwan to Nanjing Port, China. Bursaphelenchus chengi sp. n. was reared and maintained on Petri dish cultures of the fungus Botrytis cinerea. The new species is characterised by the medium body size in both sexes, the presence of only two incisures in the lateral field and the robust and strongly curved spicules. The spicule lamina is angular distally, the rostrum digitate and the condylus rounded. The tail is arcuate with a pointed terminus. The bursa is usually truncate with the posterior margin indented in some specimens or rounded with a fine axial point. Females have a small vulval flap formed by a short extension of the cuticle of the anterior lip, and a conical tail that gradually tapers to an almost straight or slightly recurved, pointed or rounded terminus. Because of the presence of two lateral lines, similar spicule shape, tapering female tail and the presence of a small vulval flap, B. chengi sp. n. should be grouped in the abietinus-group sensu Braasch. together with B. abietinus, B. antoniae, B. hellenicus, B. hylobianum and B. rainulfi. ITS-RFLP profiles support the proposal of the new species, and phylogenetic analysis of the 28S rDNA D2/D3 domain sequence places it close to B. antoniae and other species of the abietinus-group.

Paurodontella gilanica n. sp. (Nematoda: Sphaerularioidea) from Iran

Nematology ◽  
2018 ◽  
Vol 20 (5) ◽  
pp. 471-482 ◽  
Author(s):  
Ali Yaghoubi ◽  
Ebrahim Pourjam ◽  
Majid Pedram
Keyword(s):  
New Species ◽  
Posterior Probability ◽  
Small Body ◽  
Bootstrap Support ◽  
Bayesian Posterior Probability ◽  
Female Reproductive System ◽  
Lateral Field ◽  
Morphological Differences ◽  
Tail Tip ◽  
18S Rdna Sequence

Summary Paurodontella gilanica n. sp. is described and illustrated based on morphological, morphometric and molecular characters. The new species is characterised by its small body size, four lines in the lateral field, weak stylet with minute asymmetrical knobs, female reproductive system lacking a diverticulum but with small post-vulval uterine sac, elongate conoid tail with pointed, sometimes filiform, tip, males common with small spicules and cloacal bursa not reaching tail tip. Morphological differences between the new species and seven known species of the genus, namely P. asymmetrica, P. auriculata, P. balochistanica, P. minuta, P. myceliophaga, P. parapitica and P. persica, are discussed. The new species is also compared with four known species of Paurodontus having four lines in the lateral field. Using a 1123 nt long partial 18S rDNA sequence of the new species to reconstruct the phylogenetic relationships revealed that it formed a clade with members of the Sphaerulariidae and Paurodontidae. Using a 746 nt long partial sequence of the 28S rDNA D2-D3 segment revealed that P. gilanica n. sp. formed a clade with Abursanema iranicum in both Bayesian inference (BI) and maximum likelihood (ML) analyses with 0.99 Bayesian posterior probability (BPP) and 89% bootstrap support value (BS). The morphological affinities of Paurodontella and Paurodontus are discussed.

Three new species of parasitaphelenchids, Parasitaphelenchus frontalis n. sp., P. costati n. sp. and Bursaphelenchus hirsutae n. sp. (Nematoda: Aphelenchoididae), isolated from bark beetles from Japan

Nematology ◽  
2018 ◽  
Vol 20 (10) ◽  
pp. 957-1005 ◽  
Author(s):  
Natsumi Kanzaki ◽  
Taisuke Ekino ◽  
Tatsuya Ide ◽  
Hayato Masuya ◽  
Yousuke Degawa
Keyword(s):  
New Species ◽  
Bark Beetles ◽  
Body Cavity ◽  
The Body ◽  
Molecular Characterisation ◽  
Lateral Field ◽  
Female Tail ◽  
Alnus Hirsuta ◽  
Three New Species ◽  
Zelkova Serrata

Summary Three parasitaphelenchid species, Parasitaphelenchus frontalis n. sp., P. costati n. sp. and Bursaphelenchus hirsutae n. sp., are described. Parasitaphelenchus frontalis n. sp. was isolated from the body cavity of Scolytus frontalis emerging from dead logs of Zelkova serrata collected from Kanagawa, Japan, and characterised by its four-lined lateral field in the adults and dome-shaped lip region of the parasitic juveniles. Whereas the two first-mentioned species were isolated from Alniphagus costatus, B. hirsutae n. sp. emerged from dead logs of Alnus hirsuta collected from Nagano, Japan. Parasitaphelenchus costati n. sp. and B. hirsutae n. sp. were recovered from the body cavity and the underside of the elytra of their host/carrier beetle, respectively. Parasitaphelenchus costati n. sp. is characterised by its three-lined lateral field in adults and the presence of a ventrally-directed hook on the lip region of the parasitic juvenile. Bursaphelenchus hirsutae n. sp. belongs to the eggersi group of the genus and is characterised by its three-lined lateral field, dorsally truncate condylus and pointed rostrum of males, and long, smoothly tapering and strongly ventrally recurved, female tail. Molecular characterisation is provided for all three new species and their phylogenys discussed.

Description of Stegelletina lingulata sp. n. (Nematoda, Rhabditida, Cephalobidae) from xeric environments in Iran

Zootaxa ◽  
2017 ◽  
Vol 4358 (3) ◽  
pp. 462
Author(s):  
JOAQUÍN ABOLAFIA ◽  
EBRAHIM SHOKOOHI
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
Small Body ◽  
Reproductive Systems ◽  
Lateral Field ◽  
Male Tail ◽  
Line Drawings ◽  
Female Tail ◽  
Body Diameter ◽  
A New Species

A new species belonging to the genus Stegelletina, S. lingulata sp. n., is described from xeric natural areas from Iran. It is characterized by having small body (0.51–0.69 mm in females and 0.45–0.64 mm in males), cuticle slightly tessellated especially at anterior end, lateral field with three longitudinal incisures, lips with long acute process at primary axils and three more dentate processes at secondary axils, primary axils with one triangular axillar guard process and secondary axils lacking axillar processes, labial probolae long (9–11 µm) with slender peduncle lacking adaxial expansion and with convergent smooth prongs, stoma, pharynx and reproductive systems cephaloboid, excretory pore at isthmus level, spermatheca 1.1–1.4 times, and post-vulval sac 0.9–1.2 times the corresponding body diameters in length, female rectum 0.6–0.8 times anal body diameter long, female tail conoid (35–47 µm long, c = 14.0–15.6, c' = 2.1–2.7) with fine rounded terminus, male tail conoid, curved ventrad (53–59 µm long, c = 11.0–12.0, c' = 1.9–2.5) with rounded terminus, spicules 31–33 µm long with ventrally straight manubrium and gubernaculum 14–17 µm long. Measurements and illustrations (line drawings, LM and SEM) are provided for this species. In addition, comments on the morphology of the lip region of the species of the genus Stegelletina are included. 

Bursaphelenchus parantoniae n. sp. (Tylenchina: Aphelenchoididae) found in packaging wood from Belgium

Nematology ◽  
2015 ◽  
Vol 17 (10) ◽  
pp. 1141-1152 ◽  
Author(s):  
Munawar Maria ◽  
Yiwu Fang ◽  
Jie He ◽  
Jianfeng Gu ◽  
Hongmei Li
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
Phylogenetic Analyses ◽  
Precloacal Papilla ◽  
Lateral Field ◽  
Female Tail ◽  
Molecular Characters

Bursaphelenchus parantoniaen. sp. isolated in Ningbo, China, from packaging wood made ofPinussp. imported from Belgium, is described. It is characterised by a slim body (a = 32.6-51), lateral field with two lines, excretory pore locatedcaone metacorpal length posterior to median bulb, spicules stout and arcuate with triangular rostrum and flattened cucullus, and seven caudal papillae arranged as a single midventral precloacal papilla, one adcloacal pair and two postcloacal pairs. The vagina is inclined anteriorly, a vulval flap is present, the post-uterine branch extends for more than half of the vulva-anus distance, and the female tail is conical with a hyaline region and bluntly rounded terminus. Phylogenetic analyses showed thatB. parantoniaen. sp. clusters with thehylobianum-group and appears close toB. antoniae,B. chengi,B. hylobianumandB. niphades. The new species is distinguished from these species by morphology, ITS-RFLP patterns and molecular characters.

Bursaphelenchus paraluxuriosae sp. n. (Nematoda: Parasitaphelenchidae) in packaging wood from Indonesia

Nematology ◽  
2012 ◽  
Vol 14 (7) ◽  
pp. 787-798 ◽  
Author(s):  
Jianfeng Gu ◽  
Jiangling Wang ◽  
Helen Braasch ◽  
Wolfgang Burgermeister ◽  
Thomas Schröder
Keyword(s):  
New Species ◽  
Body Length ◽  
Excretory Pore ◽  
Total Body Length ◽  
Lateral View ◽  
Precloacal Papilla ◽  
Lateral Field ◽  
Female Tail ◽  
Total Body

Bursaphelenchus paraluxuriosae sp. n., isolated in Ningbo, China, from packaging wood made from Alphitonia sp. (Rhamnaceae) imported from Indonesia is described. It is characterised by a slim body (a = 29-39), lateral field with four lines, excretory pore located at level of median bulb or slightly posterior, vulva at 75% of total body length, presence of a distinct vulval flap in lateral view, post-uterine branch ca two-thirds of the vulva-anus distance, female tail conical and slightly ventrally bent with irregular or roughened terminus (c′ = 4.4), spicules large and arcuate, with pointed rostrum and a cucullus, which is typical for the xylophilus group, but is reduced to a small blunt extension, and three pairs of ventro-sublateral papillae (one pair precloacal, two pairs postcloacal just anterior to the bursal flap and adjacent to each other) and a small single precloacal papilla present. The new species belongs to the xylophilus group and is most similar to B. luxuriosae. It is distinguished from other Bursaphelenchus species by morphology, ITS-RFLP patterns and sequencing results.

Bursaphelenchus posterovulvus sp. n. (Nematoda: Parasitaphelenchidae) in packaging wood from Singapore

Nematology ◽  
2014 ◽  
Vol 16 (4) ◽  
pp. 403-410 ◽  
Author(s):  
Jianfeng Gu ◽  
Ning Wang ◽  
Jie He ◽  
Jiangling Wang ◽  
Xianfeng Chen ◽  
...  
Keyword(s):  
New Species ◽  
Body Length ◽  
Excretory Pore ◽  
Phylogenetic Analyses ◽  
Total Body Length ◽  
Precloacal Papilla ◽  
Lateral Field ◽  
Female Tail ◽  
Total Body

Bursaphelenchus posterovulvussp. n., isolated in Ningbo, P.R. China, from non-coniferous packaging wood from Singapore is described. It is characterised by a slim body (a = 24-43), lip region strongly offset, stylet with prominent and strongly sclerotised basal swellings, lateral field with four lines, excretory pore located at level of median bulb, hemizonid 30-40 μm posterior to excretory pore, vulva at 82-86% of total body length, post-uterine branch extending forcatwo-thirds of vulva-anus distance, female tail conical and short (c′ = 2.3-3.0) with a pointed terminus, spicules short (11-16 μm) and slightly arcuate, rostrum blunt and relatively short, dorsal and ventral limbs conspicuous and their distal ends separated, three pairs of ventro-sublateral papillae and a single precloacal papilla. The new species is distinguished from otherBursaphelenchusspecies by morphology, ITS-RFLP patterns and phylogenetic analyses.

Bursaphelenchus paraleoni n. sp. (Tylenchina: Aphelenchoididae) intercepted in Loblolly pine logs imported into China from the USA

Nematology ◽  
2021 ◽  
pp. 1-11
Author(s):  
Jianfeng Gu ◽  
Yiwu Fang ◽  
Lele Liu ◽  
Xinxin Ma ◽  
Xiaoling Lu
Keyword(s):  
New Species ◽  
Pinus Taeda ◽  
Loblolly Pine ◽  
Precloacal Papilla ◽  
Long Tail ◽  
Lateral Field ◽  
The Usa ◽  
Males And Females ◽  
A New Species ◽  
Huge Variation

Summary Bursaphelenchus paraleoni n. sp. was isolated from Loblolly pine, Pinus taeda, logs from the USA. The new species is characterised by a lateral field with three lines, 13-16 μm long stylet with small basal swellings, and the excretory pore located slightly posterior to the nerve ring. The male spicules are mitten-shaped, the lamina smoothly tapering towards the distal tip. The condylus is broadly rounded, with a small dorsally bent hook, rostrum triangular or conical with finely rounded tip, and cucullus absent. One midventral precloacal papilla, one adcloacal pair and two postcloacal pairs of genital papillae are present. The bursal flap is long and spade-like with the posterior margin truncate or irregular. Females have a small vulval flap 4-6 μm long, a post-vulval uterine sac extending for 34-49% of the vulva to anus distance, a very long tail with slight ventral curvature, mostly filiform and with a finely rounded terminus, occasionally bluntly rounded. Huge variation in the tails of males and females were observed in a culture. It is similar to species of B. eidmanni, B. leoni, and B. silvestris in the leoni-group but detailed phylogenetic analysis based on 18S, ITS and D2-D3 28S sequences confirmed it as a new species.

Bursaphelenchus rufipennis n. sp. (Nematoda: Parasitaphelenchinae) and redescription of Ektaphelenchus obtusus (Nematoda: Ektaphelenchinae), associates from nematangia on the hind wings of Dendroctonus rufipennis (Coleoptera: Scolytidae)

Nematology ◽  
2008 ◽  
Vol 10 (6) ◽  
pp. 925-955 ◽  
Author(s):  
Natsumi Kanzaki ◽  
Robin M. Giblin-Davis ◽  
Yasmin J. Cardoza ◽  
Weimin Ye ◽  
Kenneth F. Raffa ◽  
...  
Keyword(s):  
New Species ◽  
Morphological Characters ◽  
Morphological Examination ◽  
Dendroctonus Rufipennis ◽  
Lateral Field ◽  
The Past ◽  
Female Tail ◽  
Molecular Phylogenetic ◽  
Voucher Specimens ◽  
Tail Tip

Abstract Two species of aphelench, Bursaphelenchus rufipennis n. sp. and Ektaphelenchus obtusus, were isolated from the 'nematangia', cocoon-like structures found at the base of the hind wings of Dendroctonus rufipennis. The nematangia contained adult females of E. obtusus and the dauer juveniles of B. rufipennis n. sp. Only B. rufipennis n. sp. could be cultured on Monilinia fructicola on LGPDA (lactic acid-treated, glycerol-supplemented, potato dextrose agar). The new species of Bursaphelenchus is described and figured and some additional morphological characters are ascribed to E. obtusus, E. josephi, E. sandiaensis, E. smaelus (= E. prolobos) and E. terebranus after examination of type and/or voucher specimens. Bursaphelenchus rufipennis n. sp. has an adult body length of ca 500-1000 μm, medium a ratios (ca 25-38 for females and ca 30-40 for males), b ratios of ca 8-13 (female) and 7-11 (male), c ratios of ca 15-22 (female and male), c′ ratios of ca 3-4 (female) and ca 2-3 (male), and is characterised by three incisures in the lateral field, mitten-shaped spicules and a conical female tail that curves ventrally and possesses a variable tail tip. The new species is morphologically closest to B. corneolus, B. curvicaudatus, B. gerberae, B. paracorneolus and B. talonus. Morphological examination of type and/or voucher specimens of five Ektaphelenchus species revealed coarse transverse body annulation and three pairs of male caudal papillae (except for the two species where males are not described). Clear typological differences were observed among these five Ektaphelenchus species in the structure of the lip region, presence/absence of stylet knobs and male spicule morphology. Although these characters have not been consistently documented in the past, they may be diagnostic for species in the genus. Molecular phylogenetic analysis based on SSU and D2/D3 LSU sequences revealed that B. rufipennis n. sp. was closest to B. paracorneolus and that E. obtusus was closest to species of Ektaphelenchoides and a Cryptaphelenchus sp.

Description of Ektaphelenchus masseyi n. sp. (Rhabditida: Ektaphelenchinae) found in dead wood, from Iran

Nematology ◽  
2020 ◽  
pp. 1-12
Author(s):  
Fariba Heydari ◽  
Majid Pedram
Keyword(s):  
New Species ◽  
Large Subunit ◽  
The Body ◽  
Body Region ◽  
Lsu Rdna ◽  
Similar Species ◽  
Natural Forests ◽  
Lateral Field ◽  
Rotten Wood ◽  
Lateral Lines

Summary Ektaphelenchus masseyi n. sp. is described and illustrated. The new species was isolated from dead/rotten wood collected from natural forests in north Iran. It is characterised by 501-758 μm long females having three lines in lateral field, more or less continuous cephalic region separated from the body only by a shallow depression, relatively short tripartite stylet (total length 13-14 μm) lacking basal knobs or swellings, excretory pore at base of metacorpus or slightly anterior to it, post-vulval uterine sac 24-32 μm long, rectum and anus absent, posterior body region conical with finely rounded terminus, and males rare, with a dorsally convex and ventrally flat tail with a small mucron at terminus, 13-15 μm long spicules, and seven caudal papillae. The new species was morphologically compared with typologically similar species of Ektaphelenchus having three lines in the lateral field, namely E. joyceae, E. macrobulbosus, E. taiwanensis, and E. winteri. It was further compared with E. olitorius and E. prolobos having a similar female posterior body end shape and with an unknown number of incisures in the lateral field. It was also compared with three species currently placed under Ektaphelenchoides, namely E. andrassyi and E. poinari with three lines in the lateral field, and E. compsi with obscure lateral lines. The molecular phylogenetic relationships of the new species with other ektaphelenchid species were investigated using partial small, and large subunit ribosomal RNA gene (SSU and LSU rDNA D2-D3) sequences using the maximal number of currently sequenced ektaphelenchid and seinurid taxa.

Sign in / Sign up

Export Citation Format

Share Document