Description of Atetylenchus minor n. sp. (Tylenchina: Tylenchidae) and data on two other species of the family

Nematology ◽  
2015 ◽  
Vol 17 (8) ◽  
pp. 981-994 ◽  
Author(s):  
Ali Yaghoubi ◽  
Ebrahim Pourjam ◽  
Mohammad Reza Atighi ◽  
Majid Pedram
Keyword(s):  
New Species ◽  
Posterior Probability ◽  
Bayesian Posterior Probability ◽  
Lateral Field ◽  
Phylogenetic Studies ◽  
Sister Clade ◽  
The Family ◽  
Molecular Phylogenetic ◽  
Morphological Differences ◽  
Short Body

Three species of the family Tylenchidae, including one new and two known species, are described and illustrated. Atetylenchus minor n. sp. is characterised by its short body length of 633 (558-691) μm, MB = 45 (41-46), lateral field with four crenate incisures, stylet 12.0 (11.5-12.5) μm long, without distinct basal knobs but with swollen arms of the shaft at its base, V = 56.3 (53.7-59.3), absence of lateral vulval membranes, and conical tail 57 (51-62) μm long with a rounded tip. Morphological differences of the new species with four known species of the genus, namely A. abulbosus, A. graminus, A. amiri and A. metaporus, are discussed. Molecular phylogenetic studies of the new species using 1089 bp partial sequences of 18S rDNA revealed that the new species formed a clade with an unpublished species assigned to Psilenchus in Bayesian inference (BI) with low Bayesian posterior probability (BPP). This clade forms a sister clade to a major clade containing two species of Psilenchus and several belonolaim species and genera. The same result was achieved using maximum likelihood (ML) analysis. Morphological and molecular phylogenetic studies were also performed on two other species, Malenchus labiatus and Neothada cancellata, using 28S rDNA D2-D3 sequences. In the inferred Bayesian tree, M. labiatus formed a clade with other species of Malenchus with 28S information with maximal support (1.00 BPP), while N. cancellata formed a clade with Boleodorinae, again with maximal (1.00) BPP.

Description of a new species of the genus Anguillonema Fuchs, 1938 (Nematoda: Sphaerularioidea) with an identification key to the species

2018 ◽  
Vol 93 (04) ◽  
pp. 504-512
Author(s):  
A. Yaghoubi ◽  
E. Pourjam ◽  
M. Pedram
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Thin Body ◽  
Molecular Characteristics ◽  
Lateral Field ◽  
Phylogenetic Studies ◽  
Sister Clade ◽  
Molecular Phylogenetic ◽  
Morphological Differences ◽  
18S Rdna Sequence

AbstractAnguillonema iranicum n. sp. is described and illustrated as the second species of this genus from Iran, based on morphological, morphometric and molecular characteristics. It is identified by a short, thin body, a continuous lip region, six lines on the lateral field, a short, thin stylet, a posteriorly located pharyngo-intestinal junction to excretory pore, the presence of a post-vulval uterine sac, vulval position at 89% (87.4–89.9%) of body length, an elongate conoid tail with a rounded to pointed tip and not dorsally bent, and common functional males with short spicules and lacking a bursa. Morphological differences between the new species and the three known species of the genus, namely A. amolensis, A. crenati and A. poligraphi, are discussed. Molecular phylogenetic studies of the new species using partial 18S rDNA sequence revealed that it formed a sister clade with three species of Howardula, one species of Anguillonema and one unidentified isolate. In phylogenetic analyses using partial sequences of 28S rDNA D2-D3 segment, the new species formed a clade with two isolates of Parasitylenchus. A key to identification of Anguillonema species is also presented.

Paurodontella gilanica n. sp. (Nematoda: Sphaerularioidea) from Iran

Nematology ◽  
2018 ◽  
Vol 20 (5) ◽  
pp. 471-482 ◽  
Author(s):  
Ali Yaghoubi ◽  
Ebrahim Pourjam ◽  
Majid Pedram
Keyword(s):  
New Species ◽  
Posterior Probability ◽  
Small Body ◽  
Bootstrap Support ◽  
Bayesian Posterior Probability ◽  
Female Reproductive System ◽  
Lateral Field ◽  
Morphological Differences ◽  
Tail Tip ◽  
18S Rdna Sequence

Summary Paurodontella gilanica n. sp. is described and illustrated based on morphological, morphometric and molecular characters. The new species is characterised by its small body size, four lines in the lateral field, weak stylet with minute asymmetrical knobs, female reproductive system lacking a diverticulum but with small post-vulval uterine sac, elongate conoid tail with pointed, sometimes filiform, tip, males common with small spicules and cloacal bursa not reaching tail tip. Morphological differences between the new species and seven known species of the genus, namely P. asymmetrica, P. auriculata, P. balochistanica, P. minuta, P. myceliophaga, P. parapitica and P. persica, are discussed. The new species is also compared with four known species of Paurodontus having four lines in the lateral field. Using a 1123 nt long partial 18S rDNA sequence of the new species to reconstruct the phylogenetic relationships revealed that it formed a clade with members of the Sphaerulariidae and Paurodontidae. Using a 746 nt long partial sequence of the 28S rDNA D2-D3 segment revealed that P. gilanica n. sp. formed a clade with Abursanema iranicum in both Bayesian inference (BI) and maximum likelihood (ML) analyses with 0.99 Bayesian posterior probability (BPP) and 89% bootstrap support value (BS). The morphological affinities of Paurodontella and Paurodontus are discussed.

Description of Lelenchus brevislitus n. sp. (Nematoda: Tylenchidae), an example of a cryptic species from Iran and its phylogenetic relationships with other species in the family

Nematology ◽  
2016 ◽  
Vol 18 (8) ◽  
pp. 987-998 ◽  
Author(s):  
Mahya Soleymanzadeh ◽  
Majid Pedram ◽  
Ebrahim Pourjam ◽  
Sergio Álvarez-Ortega
Keyword(s):  
New Species ◽  
Cryptic Species ◽  
Molecular Data ◽  
Lateral View ◽  
Lateral Field ◽  
Rdna Sequences ◽  
Phylogenetic Studies ◽  
Sister Clade ◽  
The Family ◽  
28S Rdna Sequences

Lelenchus brevislitus n. sp. is described and illustrated based on morphological, morphometric and molecular data. The new species is characterised by having females with slender, 452-488 μm long, body lateral field vestigial as a plain simple band under scanning electron microscopy (SEM) in vulval region and absent in proximal and distal body ends, cephalic region short, ca 2 μm high, conical and truncate in anterior end in lateral view under SEM, dorso-ventrally flattened, amphidial pouches pocket-shaped and large under light microscopy (LM), their apertures short (ca 1 μm long in SEM) longitudinal sinuous slits remarkably wide near the small oral disc and significantly narrowing posteriad stylet, slender. 6.5-8.0 μm long, developed median bulb with distinct valvular plates, monodelphic-prodelphic reproductive system having a short post-vulval uterine sac (PUS), transverse vulval slit (V = 47.5-51.7) bearing small lateral membranes on each side, long filiform female tail (154-170 μm, c = 2.8-3.1, c′ = 21.5-28.3), and males unknown. The new species is considered to be a cryptic species inside the Tylenchidae. Morphological comparisons with all species of the genus are discussed. Phylogenetic studies were performed for further characterisation of the new species, and the results, using nearly full length 18S rDNA data, revealed it formed a clade with an isolate of L. leptosoma, both of which are in a sister clade with an isolate of Sakia. In a reconstructed phylogenetic tree using partial 28S rDNA sequences, the new species formed a monophyletic group, again with L. leptosoma, a sister clade to some Malenchus spp.

Observations on Malenchus geraerti n. sp. (Rhabditida: Tylenchidae), a morphological and molecular phylogenetic study

Zootaxa ◽  
2018 ◽  
Vol 4369 (3) ◽  
pp. 406 ◽  
Author(s):  
MAJID PEDRAM ◽  
MAHYA SOLEYMANZADEH ◽  
EBRAHIM POURJAM ◽  
MAHYAR MOBASSERI
Keyword(s):  
New Species ◽  
Large Subunit ◽  
Small Subunit ◽  
Phylogenetic Study ◽  
Molecular Characteristics ◽  
Lsu Rdna ◽  
Northern Iran ◽  
Lateral Field ◽  
Molecular Phylogenetic ◽  
Short Body

Malenchus geraerti n. sp., recovered from natural regions of northern Iran, is described and illustrated based on morphological, morphometric and molecular data. The new species is characterized by having females with a short body, an anteriorly wide S-shaped amphidial opening narrowing posteriorly, cuticle with prominent annuli, lateral field a plain band with smooth margins, muscular metacorpus with well-developed valve and corresponding plates, spermatheca filled with small spheroid sperm cells, vulva sunken in body with large epiptygma and no flap, and conical tail tapering gradually to a more or less pointed tip. Males of the new species are characterized by having a short body, tylenchoid spicules, adcloacal bursa with smooth margin and tail similar to that of the female. Morphologically, the new species is similar to five known species of the genus: M. fusiformis, M. machadoi, M. pachycephalus, M. solovjovae and M. undulatus. It most closely resembles M. pachycephalus, but as a cryptic species it can be differentiated using morphological and molecular characteristics. Comparisons with the four other aforementioned species are also discussed. Molecular phylogenetic studies using partial sequences of small and large subunit ribosomal DNA fragments reveal that the new species forms a clade with the species M. neosulcus in the small subunit (SSU) rDNA, and two species of Lelenchus in the large subunit (LSU) rDNA tree. 

Pseudaphelenchus vindai n. sp. (Tylenchomorpha: Aphelenchoididae) associated with termites (Termitidae) in Barro Colorado Island, Panama

Nematology ◽  
2010 ◽  
Vol 12 (6) ◽  
pp. 905-914 ◽  
Author(s):  
Natsumi Kanzaki ◽  
Robin M. Giblin-Davis ◽  
E. Allen Herre ◽  
Rudolf H. Scheffrahn ◽  
Barbara J. Center
Keyword(s):  
New Species ◽  
Field Survey ◽  
Barro Colorado Island ◽  
Subterranean Termites ◽  
Lateral Field ◽  
Female Tail ◽  
The Family ◽  
Molecular Phylogenetic ◽  
Pharyngeal Gland ◽  
Basal Position

Abstract In 2008, a field survey of termite-associated nematodes was conducted on Barro Colorado Island, Smithsonian Tropical Research Institute (BCI, STRI), Panama. During that survey, an undescribed Pseudaphelenchus species was isolated from three species of subterranean termites, Amitermes beaumonti, Microcerotermes exiguous and Obtusitermes panamae. The nematode is described and figured herein as P. vindai n. sp. The new species is morphologically similar to its only congener, P. yukiae, i. e., these two species share a thin stylet with small and clear basal knobs, a true bursa supported by three bursal limb-like genital papillae and a nerve ring surrounding the anterior clear region of the pharyngeal gland lobe and intestine. The molecular phylogenetic analysis based upon near full length (ca 1.7 kb) SSU ribosomal DNA sequence suggested that the new species forms a well supported clade with P. yukiae, at the basal position of the family Aphelenchoididae. The new species is distinguished from P. yukiae by possessing a clear condylus and rostrum of the capitulum and arcuate calomus/lamina complex of the spicules vs no condylus and rostrum and a relatively straight calomus/lamina complex, long and tapering female tail without small mucro vs blunt with small mucro present and possession of lateral field with three incisures vs four incisures.

The formerly enigmatic Unidentiidae in the limelight again: a new species of the genus Unidentia from Thailand (Gastropoda: Nudibranchia)

Zootaxa ◽  
2019 ◽  
Vol 4551 (5) ◽  
pp. 556 ◽  
Author(s):  
TATIANA KORSHUNOVA ◽  
RAHUL MEHROTRA ◽  
SPENCER ARNOLD ◽  
KENNET LUNDIN ◽  
BERNARD PICTON ◽  
...  
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Morphological Data ◽  
Sea Slugs ◽  
The Family ◽  
Molecular Phylogenetic ◽  
Morphological Differences ◽  
First Time ◽  
A New Species

An integrative molecular and morphological study is presented for the family Unidentiidae. Molecular phylogenetic analyses were conducted with the inclusion of all previous and newly obtained molecular data for the family Unidentiidae Millen & Hermosillo 2012. A new species of the genus Unidentia Millen & Hermosillo 2012, U. aliciae sp. nov., is described from Thailand as part of an inventory of sea slugs at Koh Tao. All up-to-date available morphological data for the species of the genus Unidentia is for the first time summarized. Morphological differences among the different species of Unidentia are clarified showing that every species has its own distinguishable morphological traits. According to the new molecular and morphological data, the family Unidentiidae is re-confirmed as a well-supported taxon of the aeolidacean nudibranchs. The taxonomy and phylogeny of the Aeolidacea in the light of the family Unidentiidae is briefly discussed and necessity of a fine-scale and narrowly-defined taxa approach instead of a ‘‘superlumping’’ one is highlighted. 

Description of Basiria khouzestanensis n. sp. (Nematoda: Tylenchidae) from Iran and its phylogenetic relationships with other species in the family

Zootaxa ◽  
2019 ◽  
Vol 4563 (3) ◽  
pp. 482 ◽  
Author(s):  
PAYAM EISVAND ◽  
REZA FARROKHI NEJAD ◽  
SEDIGHE AZIMI
Keyword(s):  
New Species ◽  
Phylogenetic Relationships ◽  
Molecular Data ◽  
Body Contour ◽  
The Family ◽  
Molecular Phylogenetic ◽  
Basal Bulb ◽  
Unidentified Species ◽  
Tail Tip ◽  
Short Body

Basiria khouzestanensis n. sp., recovered from the rhizosphere of orange in Khouzestan Province, south-western Iran, is described and illustrated based on morphological, morphometric and molecular data. The new species is characterized by having short body length (430–635 µm), lateral fields composed of four incisures, with non areolated bands, cephalic region smooth and continuous with body contour, stylet 9–12.5 µm long, basal bulb short and pyriform to slightly cylindrical, V = 63.3 (58–65.5), spermatheca non-offset, tail elongate-conoid, tail tip with various shapes and males with 16.5 (15–18) μm long spicules. Morphologically, the new species is similar to five known species of the genus: B. babhi, B. brevia, B. graminophila, B. lauta and B. shahidi. Morphological comparisons with these species are discussed. The sequence of the D2–D3 expansion segments of 28S rDNA gene for the new species was also used for molecular phylogenetic analysis. The phylogenetic relationships of the new species in relation to representatives of the family Tylenchidae, obtained from Bayesian inference (BI) analysis of the D2–D3 sequences, are presented and discussed. The new species clusters in a subclade containing three unidentified species of the genus Basiria from Iran with high support. 

scholarly journals A pot-pourri of new species of Trypetheliaceae resulting from molecular phylogenetic studies

2016 ◽  
Vol 48 (6) ◽  
pp. 639-660 ◽  
Author(s):  
Robert LÜCKING ◽  
Matthew P. NELSEN ◽  
André APTROOT ◽  
Michel N. BENATTI ◽  
Nguyen Quoc BINH ◽  
...  
Keyword(s):  
New Species ◽  
Molecular Data ◽  
Orange Pigment ◽  
Tropical Asia ◽  
Phylogenetic Studies ◽  
The Family ◽  
Molecular Phylogenetic ◽  
Yellow Orange ◽  
Thallus Surface ◽  
Its Barcoding

AbstractBased on separately obtained and analyzed molecular data and within the framework of a global revision of the family Trypetheliaceae, 21 new species are described, from the Neotropics and tropical Asia, in the genera Architrypethelium (1), Astrothelium (15), Bathelium (1), Nigrovothelium (1), Trypethelium (1), and Viridothelium (2), namely: Architrypethelium lauropaluanum Lücking, M. P. Nelsen & Marcelli sp. nov., differing from A. hyalinum in the perithecia immersed between coarse thallus verrucae and in the additional ascospore septa; Astrothelium aurantiacocinereum Lücking, Naksuwankul & Lumbsch sp. nov., differing from A. aeneum in the prominent, well-delimited, trypethelioid pseudostromata and the absence of pigment on the thallus surface, as well as in the barely lichenized thallus; A. carassense Lücking, M. P. Nelsen & Marcelli sp. nov., differing from A. purpurascens in orange, K+ red pseudostroma pigment and the slightly larger ascospores; A. cryptolucens Lücking, M. P. Nelsen & N. Salazar sp. nov., differing from A. carrascoense in the inspersed hymenium; A. fijiense Lücking, Naksuwankul & Lumbsch sp. nov., differing from A. cinereorosellum in the presence of lichexanthone on the well-delimited pseudostromata and in the slightly shorter ascospores; A. laevithallinum Lücking, M. P. Nelsen & Marcelli sp. nov., differing from A. endochryseum in the smooth thallus; A. leucosessile Lücking, M. P. Nelsen & Aptroot sp. nov., differing from A. phlyctaena in the conspicuous, sessile pseudostromata; A. macrostomoides Lücking, M. P. Nelsen & Benatti sp. nov., differing from A. macrostomum in the larger ascospores; A. megacrypticum Lücking, M. P. Nelsen & N. Salazar sp. nov., differing from A. longisporum in the single-spored asci and larger ascospores; A. nicaraguense Lücking, M. P. Nelsen & T. Orozco sp. nov., differing from A. gigantosporum in the smaller ascospores; A. norisianum Lücking, M. P. Nelsen & Aptroot sp. nov., differing from A. sepultum in the distinct, well-delimited pseudostromata; A. obtectum Lücking, M. P. Nelsen & Benatti sp. nov., differing from A. nigrocacuminum in the smaller ascospores; A. sordithecium Lücking, M. P. Nelsen & Marcelli sp. nov., differing from A. leucothelium in the inspersed hymenium and the absence of lichexanthone from the thallus surface outside the pseudostromata; A. subendochryseum Lücking, M. P. Nelsen & Marcelli sp. nov., differing from A. endochryseum in the absence of pigment in the pseudostromata and the lateral thallus cover of the pseudostromata; A. subinterjectum Lücking, M. P. Nelsen & Jungbluth sp. nov., differing from A. obtectum in the smaller pseudostromata and smaller ascospores, and from A. interjectum in the diffuse pseudostromata and smaller ascospores; Bathelium porinosporum Lücking, M. P. Nelsen & Gueidan sp. nov., differing from other Bathelium species in the 3-septate, euseptate ascospores; Nigrovothelium bullatum Lücking, Upreti & Lumbsch sp. nov., differing from N. tropicum in the bullate thallus; Trypethelium tolimense Lücking, Moncada & M. Gut. sp. nov., differing from T. xanthoplatystomum in the absence of a yellow-orange pigment on the pseudostromata and the K+ yellow (not K+ red) medullary pigment; Viridothelium tricolor Lücking, M. P. Nelsen & N. Salazar sp. nov., characterized by black perithecia with a lateral ostiole immersed in white pseudostromata strongly contrasting with the surrounding brown thallus, in combination with 2-spored asci and large, muriform ascospores; and V. vonkonratii Lücking, Naksuwankul & Lumbsch sp. nov., differing from V. virens in larger ascospores and mostly solitary ascomata. All species are illustrated and their taxonomy and phylogenetic relationships are discussed. ITS barcoding sequences are reported for five specimens of Bathelium porinosporum.

A molecular phylogenetic approach for unravelling the taxonomic status of Basilaphelenchus persicus n. gen., n. sp. (Aphelenchoididae: Tylaphelenchinae)

Nematology ◽  
2018 ◽  
Vol 20 (6) ◽  
pp. 567-582 ◽  
Author(s):  
Majid Pedram ◽  
Natsumi Kanzaki ◽  
Robin M. Giblin-Davis ◽  
Ebrahim Pourjam
Keyword(s):  
New Genus ◽  
Small Body ◽  
Taxonomic Status ◽  
Cephalic Region ◽  
Rrna Gene ◽  
Phylogenetic Studies ◽  
Sister Clade ◽  
The Family ◽  
Lsu Rrna Gene ◽  
Molecular Phylogenetic

Summary Basilaphelenchus persicus n. gen., n. sp. is described and illustrated based on morphological and molecular phylogenetic studies. The new genus belongs to the family Aphelenchoididae and is characterised mainly by the small body size of females (297-393 μm) and males (304-331 μm), high cephalic region in both sexes, fine 5.5-7.8 μm long stylet that is barely visible in mounted specimens yet clear in fresh individuals, stylet with a linear conus and weak shaft ending in three elongate, posteriorly directed, knobs, small spherical metacorpus with posteriorly located (at 72-75% of metacorpus) weak but distinct valve, monodelphic-prodelphic reproductive system with a long post-vulval uterine sac containing large rounded sperm cells, a vulva lacking any flap apparatus, conical tail of female usually ventrally bent at tip and male tail ventrally bent as in the female, lacking the P1 supplement, but with three pairs of caudal papillae, and small arcuate spicules with rounded condylus and small pointed rostrum. The new genus is similar to both Tylaphelenchus and Pseudaphelenchus in the possession of a raised cephalic region and a small spherical metacorpus, placing it in the subfamily Tylaphelenchinae. It is also typologically similar to Aphelenchoides. Molecular phylogenetic studies using both SSU and LSU rRNA gene sequences (both partial) revealed that the new genus formed a robustly supported sister clade with Pseudaphelenchus spp. and had a basal placement to this clade and, probably, to all other aphelenchoidids. Tylaphelenchus grosmannae is transferred to the new genus as B. grosmannae n. comb. and A. christinae n. comb. (= T. christinae) is proposed. The taxonomic status of Tylaphelenchus is discussed and T. sinodendroni n. comb. (= A. sinodendroni) proposed.

Morphological and molecular data of two species of the rare genera Thada Thorne, 1941 and Tenunemellus Siddiqi, 1986 (Nematoda: Tylenchidae) from Iran

2020 ◽  
Vol 94 ◽  
Author(s):  
M. Hosseinvand ◽  
A. Eskandari ◽  
R. Ghaderi ◽  
A. Karegar
Keyword(s):  
New Species ◽  
Ribosomal Dna ◽  
Large Subunit ◽  
Populus Euphratica ◽  
Molecular Data ◽  
Small Subunit ◽  
Phylogenetic Studies ◽  
Molecular Phylogenetic ◽  
Morphological Differences ◽  
Morphological And Molecular Data

Abstract Thada populus n. sp. was found in the rhizosphere of Populus euphratica in the city of Dezful, south-western Iran. The new species is characterized by its 365–453 μm body length, cuticle with transverse annuli 0.9–1.2 μm wide, lateral fields with four lines, lip region low with one or two annuli, 7.0–8.5 μm wide at base, longitudinal or slightly sigmoid amphidial slit, delicate stylet 8.4–9.8 μm long with posteriorly directed knobs, dorsal pharyngeal gland opening at 1.0–1.5 μm posterior to the stylet knobs, non-muscular and valve-less median bulb, pyriform and offset basal bulb, almost oval spermatheca, posterior position of vulva (V = 75–79%) without lateral membrane, short post-vulval uterine sac and conical tail with finely to broadly rounded terminus. Morphological differences of the new species with those of the type species, Thada striata, are discussed. Molecular phylogenetic studies of the new species using partial sequences of small subunit ribosomal DNA revealed that the new species formed a clade with Malenchus spp., Filenchus facultativus, F. fungivorus and Filenchus sp. in Bayesian inference. Morphological and molecular phylogenetic studies were also performed on Tenunemellus indicus, the second recovered species, the latter analysis using partial sequences of large subunit ribosomal DNA. In the inferred Bayesian tree, T. indicus formed a clade with Labrys fuzhouensis, Lelenchus leptosoma from the Netherlands, Malenchus spp. and Filenchus discrepans.

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