Revision of the genus Dinotoperla Tillyard, 1921 (Plecoptera: Gripopterygidae) using morphological characters and molecular data: Establishes two new genera, three new species and updates the larval taxonomy

Zootaxa ◽  
2017 ◽  
Vol 4224 (1) ◽  
pp. 1
Author(s):  
JULIA H MYNOTT ◽  
PHILLIP J SUTER ◽  
GUNTHER THEISCHINGER
Keyword(s):  
New Species ◽  
Mitochondrial Gene ◽  
Life Stage ◽  
Morphological Characters ◽  
Molecular Data ◽  
Morphological Identification ◽  
New Genera ◽  
Adult Males ◽  
Larval Life ◽  
Dichotomous Key

The larval taxonomy of Australian stoneflies (Plecoptera) shows a large disparity in knowledge when compared to the adult taxonomy with many species having undescribed larval forms. The importance of stoneflies as an indicator group for monitoring aquatic ecosystems means knowledge of the larval taxonomy and the ability to identify species is essential. This study combined morphology and mitochondrial gene sequences to associate the adult and larval life-stages for species of Dinotoperla Tillyard. Morphological identification of adult males was recognised for 17 of the 35 Dinotoperla species and combining molecular data with morphology confirmed eight new adult-larval life stage associations. Further, molecular data supported the larval taxonomy for five morphospecies which remain unassociated. The combination of molecular and morphological methods enabled the larval morphology to be reassessed for the genus Dinotoperla and this has led to the establishment of two new genera, Odontoperla, gen. nov. and Oedemaperla, gen. nov., and the new species Dinotoperla aryballoi, sp. nov, D. tasmaniensis, sp. nov. and Oedemaperla shackletoni, sp. nov. as well as the new or updated descriptions of the larvae of 31 species and a comprehensive dichotomous key to these larvae. 

New species and findings of Pagastia Oliver (Diptera: Chironomidae: Diamesinae) from Central Asia, with DNA barcoding of known species of the genus

Zootaxa ◽  
2021 ◽  
Vol 4951 (3) ◽  
pp. 559-570
Author(s):  
EUGENYI A.  MAKARCHENKO ◽  
ALEXANDER A. SEMENCHENKO ◽  
DMITRY M. PALATOV
Keyword(s):  
New Species ◽  
Central Asia ◽  
Cytochrome Oxidase ◽  
Phylogenetic Trees ◽  
Morphological Characters ◽  
Molecular Data ◽  
Adult Males ◽  
Cytochrome Oxidase Subunit ◽  
Oxidase Subunit ◽  
Coi Sequences

Chironomids of the genus Pagastia Oliver (Diamesinae, Diamesini) from the mountains of Central Asia are revised using both morphological characters and molecular data. Illustrated descriptions of the adult male Pagastia (P.) caelestomontana sp. nov. from Kirgizstan and Tajikistan, P. (P.) hanseni sp. nov. from Tajikistan, and record of a finding apparently a new species P. (P.) aff. lanceolata (Tokunaga) from Tajikistan as well as an updated a key to the determination of the adult males of all known species of Pagastia are provided. A phylogenetic framework is reconstructed based on two mitochondrial genes cytochrome oxidase subunit I (COI) sequences of 34 samples belonging to 7 species of the genus Pagastia and cytochrome oxidase subunit II (COII) available for most samples. Phylogenetic trees of some known species of the genus Pagastia were reconstructed using the combined dataset and Bayesian inference (BI) and Maximum Likelihood (ML) methods. The interspecific K2P distances between seven Pagastia species including P. (P.) caelestomontana sp. nov., P. (P.) hanseni sp. nov. and undescribed P. (P.) aff. lanceolata (Tokunaga) are 6.3–13.2 which corresponding to species level. 

Morphological and molecular study of the torrent catfishes (Sisoridae: Glyptosterninae) of Bhutan including the description of five new species

Zootaxa ◽  
2018 ◽  
Vol 4476 (1) ◽  
pp. 40
Author(s):  
RYAN J. THONI ◽  
DHAN B. GURUNG
Keyword(s):  
New Species ◽  
Mitochondrial Gene ◽  
Nuclear Gene ◽  
Molecular Study ◽  
Molecular Data ◽  
Cyt B ◽  
Dichotomous Key ◽  
Morphological And Molecular Data

Torrent catfishes of the subfamily Glyptosterninae from Bhutan are examined based on morphological and molecular data. Five new species are described: Creteuchiloglanis bumdelingensis sp. nov., Exostoma mangdechhuensis sp. nov., Parachiloglanis benjii sp. nov., P. dangmechhuensis sp. nov., and P. drukyulensis sp. nov. Molecular data derived from the mitochondrial gene Cyt b and the nuclear gene RAG2 recovered relationships within Parachiloglanis and the Glyptosterninae. A dichotomous key to the Glyptosterninae of Bhutan is provided.

scholarly journals Integrative taxonomy reveals two new cryptic species of Hyphessobrycon Durbin, 1908 (Teleostei: Characidae) from the Maracaçumé and middle Tocantins River basins, Eastern Amazon region

2020 ◽  
Vol 723 ◽  
pp. 77-107
Author(s):  
Erick Cristofore Guimarães ◽  
Pâmella Silva De Brito ◽  
Pedro Henrique Negreiros Bragança ◽  
Jadson Pinheiro Santos ◽  
Axel Makay Katz ◽  
...  
Keyword(s):  
New Species ◽  
Mitochondrial Gene ◽  
Morphological Characters ◽  
Molecular Data ◽  
Color Pattern ◽  
Integrative Taxonomy ◽  
The Body ◽  
Dorsal Fin ◽  
Two New Species ◽  
Molecular Phylogenetic

Two new species, Hyphessobrycon frickei Guimarães, Brito, Bragança, Katz & Ottoni sp. nov. and H. geryi Guimarães, Brito, Bragança, Katz & Ottoni sp. nov., are herein described, based on seven different and independent species delimitation methods, and on molecular and morphological characters, making the hypothesis of these new species supported from an integrative taxonomy perspective. They belong to the “Rosy tetra” clade, which is mainly characterized by the presence of a dark brown or black blotch on the dorsal fin and the absence of a midlateral stripe on the body. These two new species are distinguished from the other members of this clade mainly by the arrangement, shape and color pattern of humeral and dorsal-fin spots, as well as by other characters related to scale counts and body pigmentation. The placement of the new species within the “Rosy tetra” clade was based on the combination of morphological character states mentioned above and corroborated by a molecular phylogenetic analysis using the mitochondrial gene cytochrome oxidase subunit 1. In addition, a new clade (here termed Hyphessobrycon copelandi clade) within the “Rosy tetra” clade is proposed based on molecular data, comprising H. copelandi, H. frickei sp. nov., H. geryi sp. nov. and a still undescribed species. Our results corroborate the occurrence of hidden species within the “Rosy tetra” clade, as suggested by previous studies.

scholarly journals Review of the millipede genus Orthomorpha Bollman, 1893 (Diplopoda, Polydesmida, Paradoxosomatidae) in Vietnam, with several new records and descriptions of two new species

ZooKeys ◽  
2019 ◽  
Vol 898 ◽  
pp. 121-158
Author(s):  
Natdanai Likhitrakarn ◽  
Sergei I. Golovatch ◽  
Irina Semenyuk ◽  
Boris D. Efeykin ◽  
Somsak Panha
Keyword(s):  
New Species ◽  
New Records ◽  
Mitochondrial Gene ◽  
Single Gene ◽  
Morphological Characters ◽  
Molecular Data ◽  
The Other ◽  
Current Usage ◽  
Two New Species ◽  
Morphological And Molecular Data

The genus Orthomorpha is shown to currently be represented in Vietnam by ten species or varieties, including new records of O. arboricola (Attems, 1937), O. coarctata (de Saussure, 1860), O. rotundicollis (Attems, 1937) and O. scabra Jeekel, 1964, and two new species, O. caramelsp. nov. and O. vietnamicasp. nov. A key to all eight Orthomorpha species and two varieties known to occur in Vietnam is provided. Although the morphological characters that have been traditionally used for Orthomorpha taxonomy are here considered superior to molecular ones, molecular-based phylogenetic relationships and taxon assignments within the tribe Orthomorphini are provisionally analyzed using fragments of the cytochrome c oxidase subunit I (COI) mitochondrial gene. The preferred phylograms, both rooted and unrooted, demonstrate the monophyly of the tribe Orthomorphini, but due to the special, uncertain or even controversial position of O. coarctata, which occurs closer to the genera Antheromorpha and Hylomus, the genus Orthomorpha in current usage appears to be polyphyletic. However, if O. coarctata is to be treated within the monotypic genus Asiomorpha, the monophyly of Orthomorpha becomes manifest. On the other hand, a cautious approach is followed to avoid descriptions of suspicious new taxa/species. Thus, solely because the average genetic distance between O. rodundicollis subrotundicollisvar. nov. and O. rodundicollis, as well as that between O. scabra grandisvar. nov. and O. scabra, are both found to be negligibly small, the statuses of the sympatric and closest yet morphologically different varieties are treated only as such, i.e., infrasubspecific categories. The apparent discord observed between morphological and molecular data is obviously due to only partial and single-gene topologies used, possibly also to hybridization already known to occur in some closely related and sympatric paradoxosomatid species or even genera.

Mitochondrial DNA allows the association of life stages to facilitate species recognition and delimitation in Australian stoneflies (Plecoptera : Gripopterygidae : Newmanoperla)

2015 ◽  
Vol 29 (3) ◽  
pp. 223 ◽  
Author(s):  
Julia H. Mynott
Keyword(s):  
Aquatic Ecosystems ◽  
Species Recognition ◽  
Mitochondrial Gene ◽  
Morphological Characters ◽  
Immature Stages ◽  
Life Stages ◽  
Larval Morphology ◽  
Larval Life ◽  
Intraspecific Divergence ◽  
Dichotomous Key

The larvae of stoneflies (Plecoptera) are important indicators for monitoring aquatic ecosystems, but the immature stages of some relevant species have not been described. Here, mitochondrial gene sequences are used to associate the adult and larval life stages for species of Newmanoperla McLellan. This study finds molecular and morphological support for five species, which include the four previously described species (N. exigua, N. hackeri, N. prona and N. thoreyi) and a newly recognised species, N. theischingeri, sp. nov., which is described herein. Molecular divergences between species for the COI fragment had minimum values of 15–18% while the maximum intraspecific divergence was 6–9%, and there was no overlap between species. Morphological characters for distinguishing the larvae of the five species were observed on the femora and included variations in the type of setation present and the area of occurrence. The combination of molecular and morphological methods enabled the larval morphology to be reassessed and has led to the following outcomes: the first formal generic larval description, a newly recognised species, updated descriptions for larvae of all species of Newmanoperla and a dichotomous key to larvae.

Punctulini (Hemiptera: Cicadellidae: Deltocephalinae), a new leafhopper tribe from the Oriental region and Pacific Islands

Zootaxa ◽  
2017 ◽  
Vol 4226 (2) ◽  
pp. 229 ◽  
Author(s):  
W. DAI ◽  
J.N. ZAHNISER ◽  
C.A. VIRAKTAMATH ◽  
M.D. WEBB
Keyword(s):  
New Species ◽  
Pacific Islands ◽  
Phylogenetic Analyses ◽  
Morphological Characters ◽  
Molecular Data ◽  
New Genera ◽  
Oriental Region ◽  
New Tribe ◽  
Morphological And Molecular Data

A new tribe of the leafhopper subfamily Deltocephalinae, Punctulini tribe nov., from China, India, Thailand, and Fiji is described and includes three new genera and five new species: Punctulus gen. nov. includes P. tumidifrons sp. nov. from China, P. manipurensis from India, and P. lobatus sp. nov. from Thailand; Hirsutula gen. nov. includes H. rubrifrons sp. nov. from Thailand; and Taveunius gen. nov. includes T. megapunctatus sp. nov., from Fiji. A key to the genera of Punctulini is provided. Observations on the shared morphological characters of Punctulini with the superficially similar tribe Magnentiini, and with Vartini to which it is found to be related based on phylogenetic analyses of morphological and molecular data including newly available sequences and analyses, are made and discussed. Afrovarta Zahniser 2013 is removed from Vartini and placed in Athysanini, placement nov. 

New lineages within the Malagasy freshwater crab fauna: Agora n. gen. for Thelphusa goudoti H. Milne Edwards, 1853, and a phytotelmic new species of Malagasya Cumberlidge & Sternberg, 2002 (Brachyura: Potamonautidae: Deckeniinae)

2020 ◽  
Vol 40 (5) ◽  
pp. 584-599
Author(s):  
Neil Cumberlidge ◽  
Julia B Soma ◽  
Ellen M Leever ◽  
Savel R Daniels
Keyword(s):  
New Species ◽  
Phylogenetic Tree ◽  
Morphological Characters ◽  
Molecular Data ◽  
The Other ◽  
New Genera ◽  
Freshwater Crab ◽  
Tropical Island ◽  
Freshwater Crabs ◽  
A New Species

Abstract New molecular and morphological analyses of the relationships of 77 specimens of potamonautid freshwater crabs collected from all parts of Madagascar support the monophyly of the island’s freshwater crab fauna and verify most of the existing taxa. The phylogenetic tree identified several new lineages that represent four new genera and 10 new species, and found Foza Reed & Cumberlidge, 2006 to be paraphyletic. The present work is the first of a series of articles reporting on these discoveries that provide an improved understanding of the relationships between the endemic potamonautid species found on this tropical island. We describe a new species of Malagasya Cumberlidge & Sternberg, 2002 from a phytotelmic habitat in northeast Madagascar, and establish Agoragen. nov. to accommodate Thelphusa goudoti H. Milne Edwards, 1853. Both of these new taxa are recognized based on combinations of morphological characters and molecular data. The new species of Malagasya is distinguished from the other two species of this genus, which are illustrated here for comparative purposes. Agora goudotin. comb. is endemic to Madagascar, and is compared with and distinguished from the eight other genera of freshwater crabs occurring in that country.

Morphological description and DNA barcoding of some Diamesinae (Diptera, Chironomidae) from the Severnaya Zemlya Archipelago and the Vaigach Island (Russian Arctic)

Zootaxa ◽  
2020 ◽  
Vol 4802 (3) ◽  
pp. 587-600
Author(s):  
ANDREY B. KRASHENINNIKOV ◽  
EUGENYI A. MAKARCHENKO ◽  
ALEXANDER A. SEMENCHENKO ◽  
MARIA V. GAVRILO ◽  
KRISTINA A. VSHIVKOVA
Keyword(s):  
Mitochondrial Gene ◽  
Interspecific Variation ◽  
Morphological Characters ◽  
Molecular Data ◽  
Genetic Distances ◽  
Morphological Description ◽  
Russian Arctic ◽  
Adult Males ◽  
Severnaya Zemlya ◽  
Severnaya Zemlya Archipelago

Chironomids of the Diamesinae subfamily from the Russian Arctic were studied using both morphological characters and molecular data. Adult males of Diamesa urvantsevi sp. nov., D. amplexivirilia Hansen, Arctodiamesa appendiculata (Lundström) from Severnaya Zemlya Archipelago and D. arctica (Boheman), Pseudokiefferiella sp. from Vaigach Island were described, redescribed, annotated and figured. A reference 658 bp barcode sequence from a fragment of the mitochondrial gene cytochrome oxidase I (COI) was used as a tool for species delimitation. For D. arctica (Boheman) and Pseudokiefferiella sp. close DNA barcodes from Norway were performed, which allowed to relate these specimens to the described species. Comparisons with corresponding regions of COI between each described species and close related congeneric species produced K2P genetic distances of 0.11–0.16, values well associated with interspecific variation. Phylogenetic relationships for genera Arctodiamesa Makarchenko and Pseudokiefferiella Zavřel were reconstructed for the first time. 

Three new species, two new genera, and new family Biphragmosagittidae (Сhaetognatha) from Southwest Pacific Ocean

2011 ◽  
Vol 20 (1) ◽  
pp. 161-173
Author(s):  
A.P. Kassatkina
Keyword(s):  
New Species ◽  
Type Species ◽  
Morphological Characters ◽  
The Body ◽  
New Order ◽  
New Genera ◽  
New Family ◽  
The Family ◽  
Three New Species

Resuming published and own data, a revision of classification of Chaetognatha is presented. The family Sagittidae Claus & Grobben, 1905 is given a rank of subclass, Sagittiones, characterised, in particular, by the presence of two pairs of sac-like gelatinous structures or two pairs of fins. Besides the order Aphragmophora Tokioka, 1965, it contains the new order Biphragmosagittiformes ord. nov., which is a unique group of Chaetognatha with an unusual combination of morphological characters: the transverse muscles present in both the trunk and the tail sections of the body; the seminal vesicles simple, without internal complex compartments; the presence of two pairs of lateral fins. The only family assigned to the new order, Biphragmosagittidae fam. nov., contains two genera. Diagnoses of the two new genera, Biphragmosagitta gen. nov. (type species B. tarasovi sp. nov. and B. angusticephala sp. nov.) and Biphragmofastigata gen. nov. (type species B. fastigata sp. nov.), detailed descriptions and pictures of the three new species are presented.

scholarly journals An elusive new species of gymnophthalmid lizard (Cercosaurinae, Selvasaura) from the Andes of northern Peru

2021 ◽  
Vol 5 (2) ◽  
pp. 177-187
Author(s):  
Lourdes Y. Echevarría ◽  
Pablo J. Venegas ◽  
Luis A. García-Ayachi ◽  
Pedro M. Sales Nunes
Keyword(s):  
New Species ◽  
Natural History ◽  
Phylogenetic Analyses ◽  
Morphological Characters ◽  
The Other ◽  
Adult Males ◽  
The Andes ◽  
New States ◽  
Northern Peru ◽  
A New Species

We describe a new species of Selvasaura from the montane forests of the eastern slopes of the Andes in northern Peru, based on external and hemipenial morphological characters and previous phylogenetic analyses. The new species can be differentiated from the other two Selvasaura species in having keeled dorsal scales usually flanked by longitudinal striations, in adults and juveniles; adult males with a yellow vertebral stripe bordered by broad dark brown stripes on each side and a unilobed hemipenis surrounded by the branches of the sulcus spermaticus. The description of the new species contributes information about new states of diagnostic characters of Selvasaura and natural history.

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