AbstractThree new species of the genus Ablechroiulus are described from natural areas in the SE Iberian Peninsula. Ablechroiulus castaneanus sp. n. is characterised by a body length of 0.69-0.80 mm in females and 0.58-0.76 mm in males, lip region offset by a shallow constriction and with six rounded lips bearing setae, stoma 18-20 μm long, metacorpus cylindrical, vulva prominent with high lips, female tail (45-52 μm, c = 12.5-15.6, c′ = 2.5-2.7) divided into two distinct regions, phasmid at base of anterior region of tail, male tail (39-63 μm, c = 12.1-14.8, c′ = 2.6-2.7) with posterior filiform part and acute terminus, bursa leptoderan with nine pairs of papillae (1 + 2 + 1 + 2 + 3), spicules 21-25 μm long and gubernaculum 10-15 μm long. Ablechroiulus querquetulanus sp. n. is characterised by a body length of 1.2-1.5 mm in females and 1.4 mm in male, stoma 24-31 μm long, procorpus 1.5 times longer than metacorpus, female tail filiform (262-318 μm, c = 4.2-4.7, c′ = 9-11), male tail (180-228 μm, c = 6.2-7.8, c′ = 3.8-5.4) with very long posterior filiform part, bursa leptoderan with nine pairs of papillae (1 + 2 + 1 + 2 + 1 + 2) of which papillae 2-3 and 5-6 are fused at base, spicules 63-72 μm long and fused at terminus and gubernaculum 32-46 μm long. Ablechroiulus verminosus sp. n. is characterised by a body length of 0.76-1.10 mm in females and 0.83-0.97 mm in males, lip region with six rounded lips bearing three setae on each side, stoma 18-26 μm long, female tail filiform (88-167 μm, c = 6.6-8.7, c′ = 5.0-7.1), male tail (63-81 μm, c = 11.0-13.3, c′ = 2.7-3.2) with posterior filiform part, bursa leptoderan with nine pairs of papillae (1 + 2 + 1 + 2 + 3) of which papillae 5-6 are fused at base, spicules 29-42 μm long, and gubernaculum 10-24 μm long. SEM pictures are provided for A. verminosus sp. n. An identification key to species of the genus as well as a tabular compendium are also provided.