A new species of the genus Eudorylaimus Andrássy, 1959 (Nematoda: (Dorylaimida: Qudsianematidae) associated with Picea crassifolia in China

Zootaxa ◽  
2018 ◽  
Vol 4526 (4) ◽  
pp. 576
Author(s):  
WEN-JIA WU ◽  
CHUN-LING XU ◽  
DONG-WEI WANG ◽  
HUI XIE
Keyword(s):  
New Species ◽  
Rhizosphere Soil ◽  
Phylogenetic Analyses ◽  
Small Subunit ◽  
Neck Length ◽  
Picea Crassifolia ◽  
Body Diameter ◽  
Small Subunit Rdna ◽  
C Value ◽  
A New Species

A new species, Eudorylaimus piceae n. sp., extracted from rhizosphere soil of Picea crassifolia from Inner Mongolia, China was identified. The new species is characterized by these combined characters: body length of 1.03–1.27 mm; lip region distinctly offset; odontostyle 20–22 μm and 1.1–1.4 times the lip region diameter in length; odontophore 1.1–1.2 times the odontostyle length; basal expansion of pharynx occupying 42%–50.5% of the total neck length; genital system didelphic-amphidelphic; vulva transverse; vagina extending inwards 32%–43%; V value averaging more than 60; pars refringens vaginae with two trapezoidal sclerotizations and pars distalis vaginae weakly sclerotized; prerectum 72–107 μm long, 2.3–3.3 times the anal body diameter, and rectum 1–1.6 times the anal body diameter in length; tail conoid and bent ventrally, c’ value 1.5–1.8 and males unknown. Phylogenetic analyses based on sequences of 18S small subunit rDNA and the D2-D3 expansion region of 28S rDNA are presented. 

scholarly journals Morphology, genetic characterization and molecular phylogeny of pinworm Skrjabinema longicaudatum n. sp. (Oxyurida: Oxyuridae) from the endangered Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae)

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Yi-Fan Cao ◽  
Hui-Xia Chen ◽  
Yang Li ◽  
Dang-Wei Zhou ◽  
Shi-Long Chen ◽  
...  
Keyword(s):  
New Species ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Small Subunit ◽  
Sister Relationship ◽  
Tibetan Antelope ◽  
A New Species

Abstract Background The Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae) is an endangered species of mammal endemic to the Qinghai-Tibetan Plateau. Parasites and parasitic diseases are considered to be important threats in the conservation of the Tibetan antelope. However, our present knowledge of the composition of the parasites of the Tibetan antelope remains limited. Methods Large numbers of nematode parasites were collected from a dead Tibetan antelope. The morphology of these nematode specimens was observed using light and scanning electron microscopy. The nuclear and mitochondrial DNA sequences, i.e. small subunit ribosomal DNA (18S), large subunit ribosomal DNA (28S), internal transcribed spacer (ITS) and cytochrome c oxidase subunit 1 (cox1), were amplified and sequenced for molecular identification. Moreover, phylogenetic analyses were performed using maximum likelihood (ML) inference based on 28S and 18S + 28S + cox1 sequence data, respectively, in order to clarify the systematic status of these nematodes. Results Integrated morphological and genetic evidence reveals these nematode specimens to be a new species of pinworm Skrjabinema longicaudatum (Oxyurida: Oxyuridae). There was no intraspecific nucleotide variation between different individuals of S. longicaudatum n. sp. in the partial 18S, 28S, ITS and cox1 sequences. However, a high level of nucleotide divergence was revealed between the new species and its congeners in 28S (8.36%) and ITS (20.3–23.7%) regions, respectively. Molecular phylogenetic results suggest that the genus Skrjabinema should belong to the subfamily Oxyurinae (Oxyuroidea: Oxyuridae), instead of the subfamily Syphaciidae or Skrjabinemiinae in the traditional classification, as it formed a sister relationship to the genus Oxyuris. Conclusions A new species of pinworm Skrjabinema longicaudatum n. sp. (Oxyurida: Oxyuridae) is described. Skrjabinema longicaudatum n. sp. represents the first species of Oxyurida (pinworm) and the fourth nematode species reported from the Tibetan antelope. Our results contribute to the knowledge of the species diversity of parasites from the Tibetan antelope, and clarify the systematic position of the genus Skrjabinema.

scholarly journals Taxonomy and SSU rDNA-Based Phylogeny of Two Heterotrich Ciliates (Ciliophora, Heterotrichea) Collected From Subtropical Wetlands of China, Including the Description of a New Species, Linostomella pseudovorticella n. sp.

2021 ◽  
Vol 12 ◽  
Author(s):  
Didi Jin ◽  
Xuetong Zhao ◽  
Tingting Ye ◽  
Jie Huang ◽  
Alan Warren ◽  
...  
Keyword(s):  
New Species ◽  
Food Web ◽  
Phylogenetic Analyses ◽  
Small Subunit ◽  
Ssu Rdna ◽  
Microbial Food Web ◽  
Model Organisms ◽  
Rdna Sequences ◽  
The Family ◽  
A New Species

The Heterotrichea Stein, 1859 are a group of ciliated protists (single-celled eukaryotes) that occur in a wide variety of aquatic habitat where they play important roles in the flow of nutrients and energy within the microbial food web. Many species are model organisms for research in cytology and regenerative biology. In the present study, the morphology and phylogeny of two heterotrich ciliates, namely, Linostomella pseudovorticella n. sp. and Peritromus kahli Villeneuve-Brachon, 1940, collected from subtropical wetlands of China, were investigated using morphological and molecular methods. L. pseudovorticella n. sp. differs from its only known congener, Linostomella vorticella Ehrenberg, 1833 Aescht in Foissner et al., 1999, by having more ciliary rows (48–67, mean about 56 vs. 26–51, mean about 42) and its small-subunit (SSU) rDNA sequence, which shows a 15-bp divergence. Although P. kahli has been reported several times in recent decades, its infraciliature has yet to be described. A redescription and improved diagnosis of this species based on a combination of previous and present data are here supplied. Phylogenetic analyses based on SSU rDNA sequences revealed that the genus Linostomella is positioned within Condylostomatidae, and Peritromidae is sister to Climacostomidae with relatively low support, and the family Spirostomidae is the root branch of the class Heterotrichea.

Morphological and molecular data on a new species of Plagiorhynchus Lühe, 1911 (Acanthocephala: Plagiorhynchidae) from the long-billed curlew (Numenius americanus) from northern Mexico

2019 ◽  
Vol 94 ◽  
Author(s):  
M. García-Varela ◽  
J.-K. Park ◽  
J.S. Hernández-Orts ◽  
C.D. Pinacho-Pinacho
Keyword(s):  
New Species ◽  
Marine Mammals ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Molecular Data ◽  
Small Subunit ◽  
Nuclear Ribosomal Dna ◽  
Northern Mexico ◽  
Numenius Americanus ◽  
A New Species

Abstract A new species of the genus Plagiorhynchus Lühe, 1911 from the intestine of the long-billed curlew (Numenius americanus) from northern Mexico is described. Plagiorhynchus (Plagiorhynchus) aznari n. sp. is morphologically distinguished from other congeneric species from the Americas by having a trunk expanded anteriorly and a cylindrical proboscis, armed with 19 longitudinal rows of hooks, with 14–15 hooks each row. Nearly complete sequences of the small subunit and large subunit of the nuclear ribosomal DNA of the new species were determined and compared with available sequences from GenBank. Phylogenetic analyses inferred from the two molecular markers consistently showed that P. (Plagiorhynchus) aznari n. sp. is closely related to P. (Plagiorhynchus) allisonae, and this clade is sister to a clade formed by P. (Prosthorhynchus) transversus and P. (Prosthorhynchus) cylindraceus from Plagiorhynchidae. The new species represents the second record of the genus in Mexico and the fourth species in the Americas. The phylogenetic relationships among the members of the order Polymorphida in this study provide significant insights into the evolution of ecological associations between parasites and their definitive hosts. Our analyses suggest that the colonization of marine mammals, fish-eating birds and waterfowl in Polymorphidae might have occurred independently, from a common ancestor of Centrorhynchidae and Plagiorhynchidae that colonized terrestrial birds and mammals.

Identification of the new species Comatricha macrospora and two other recently recorded species of Comatricha from China

Phytotaxa ◽  
2018 ◽  
Vol 374 (1) ◽  
pp. 71 ◽  
Author(s):  
BO ZHANG ◽  
SAIYU WANG ◽  
XIAOQI XU ◽  
TIANHAO LI ◽  
DAN DAI ◽  
...  
Keyword(s):  
New Species ◽  
Ribosomal Rna ◽  
Phylogenetic Analyses ◽  
Small Subunit ◽  
Jiangxi Province ◽  
Scanning Electron Micrographs ◽  
Rna Sequences ◽  
Ribosomal Rna Sequences ◽  
First Time ◽  
A New Species

A new species (Comatricha macrospora) has been collected from the Changbai Mountain National Nature Reserve, Jilin Province, China. Comatricha macrospora has smaller sporocarps and larger spores (about 15–18 μm in diameter) than other species of Comatricha as well as a persistent peridium at the base of the sporotheca. In addition, two newly recorded species of Comatricha—C. tenerrima (M.A. Curtis) G. Lister and C. afroalpina Rammeloo—have been documented in China for the first time, based on material collected from northeast China and the campus of East China Normal University, Fuzhou City, Jiangxi Province. Comatricha tenerrima is characterised by fusiform long-stalked sporocarps and warted pinkish brown spores (about 7–8 μm in diameter)., whereas C. afroalpina occurs on rotting logs and has spores marked by larger warts with an irregular reticulation at their base. Descriptions and scanning electron micrographs for these members of the genus Comatricha are provided. Phylogenetic analyses, based on small subunit ribosomal RNA sequences (SSUrRNA) of Comatricha and related genera, were carried out using Bayesian inference. These analyses confirmed the placement of the new species in the genus Comatricha.

Phialocephala sphaeroides sp. nov., a new species among the dark septate endophytes from a boreal wetland in Canada

2004 ◽  
Vol 82 (5) ◽  
pp. 607-617 ◽  
Author(s):  
B J Wilson ◽  
H D Addy ◽  
A Tsuneda ◽  
S Hambleton ◽  
R S Currah
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Small Subunit ◽  
Bromocresol Purple ◽  
Internal Transcribed Spacer Region ◽  
Sequence Comparisons ◽  
Yellow Colour ◽  
Prolonged Incubation ◽  
Phialocephala Fortinii ◽  
A New Species

Dark septate root endophytic fungi from plants growing on either side of an abrupt wetland–upland ecotone included isolates of Phialocephala fortinii Wang & Wilcox, Leptodontidium orchidicola Sigler & Currah, Hetero conium chaetospira (Grove) Ellis, and a hitherto undescribed fungus resembling P. fortinii. Six isolates of this species were recovered and were distinctive in (i) producing an orange–tan diffusible pigment in culture, (ii) causing a yellow colour shift on casamino acids medium containing bromocresol purple, (iii) having the ability to liquefy gelatin, and microscopically, (iv) forming hyaline conidia from phialides arranged in large spherical heads after prolonged incubation at 5 °C. First-formed or primary conidia are bullet shaped, 1–1.5 µm × 2–3 µm; subsequent conidia are spherical and 1–1.5 µm in diameter. Small subunit and internal transcribed spacer region sequence comparisons with P. fortinii and other Phialocephala species supported placing these six unique strains in a new species, Phialocephala sphaeroides B.J. Wilson sp. nov. Phylogenetic analyses also suggest that P. sphaeroides is affiliated with mollisioid taxa in the Dermateaceae. In contrast with P. fortinii, which was isolated on both sides of the ecotone, P. sphaeroides was obtained only from plants in the highly acidic, Sphagnum-dominated wetland habitat and not from the same species in the less acidic, aspen-dominated upland site.Key words: inoperculate discomycetes, phialidic anamorph, Helotiales, root endophytes, Loramycetaceae, Dermateaceae, Mollisia.

A new species of the genus Aporcelinus Andrássy, 2009 (Nematoda, Dorylaimida, Aporcelaimidae) from Costa Rica

Zootaxa ◽  
2018 ◽  
Vol 4450 (4) ◽  
pp. 489 ◽  
Author(s):  
INGRID VARELA-BENAVIDES ◽  
REYES PEÑA-SANTIAGO
Keyword(s):  
New Species ◽  
Costa Rica ◽  
Neck Length ◽  
Natural Habitats ◽  
Body Diameter ◽  
A New Species

A new species of the genus Aporcelinus, A. elongicaudatus sp. n., collected from natural habitats of Costa Rica, is described and illustrated. The new species is characterized by its 0.92–1.05 mm long body, lip region offset by weak constriction and 15 µm broad, odontostyle 15–18 µm or 1.0–1.2 times the lip region diameter, neck 270–300 µm long, pharyngeal expansion 130–150 µm long or 45–55% of total neck length, uterus 33–41 µm long or 0.70–0.85 times the corresponding body diameter (but certainly longer), vulva transverse (V = 49–51), and tail conical elongated (45–71 µm, c = 14–22, c’ = 2.0–3.2) and dorsad bent. 

Filsoniana lhasanensis sp. nov. from Tibet, China

Mycotaxon ◽  
2020 ◽  
Vol 134 (4) ◽  
pp. 663-675
Author(s):  
Xue-Mei Wen ◽  
Hurnisa Shahidin ◽  
Abdulla Abbas
Keyword(s):  
New Species ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Small Subunit ◽  
Dark Orange ◽  
A New Species

A new species, Filsoniana lhasanensis, is described from Tibet, China, where it grows on rocks at altitudes 4200–4432 m. The lichen is characterised by a poorly developed or absent dark orange areolate thallus, lecanorine to zeorine orange to deep-orange aggregated apothecia, and richly branched and anastomosed paraphyses. DNA was obtained for three gene loci—nuclear internal transcribed spacer (ITS), nuclear large subunit (LSU), and mitochondrial small subunit (SSU). Phylogenetic analyses support the taxon as a new species of Filsoniana .

scholarly journals Morphology, genetic characterization and molecular phylogeny of pinworm Skrjabinema longicaudatum sp. n. (Oxyurida: Oxyuridae) from the endangered Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae)

2020 ◽  
Author(s):  
Yi-Fan Cao ◽  
Hui-Xia Chen ◽  
Yang Li ◽  
Dang-Wei Zhou ◽  
Shi-Long Chen ◽  
...  
Keyword(s):  
New Species ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Small Subunit ◽  
Sister Relationship ◽  
Tibetan Antelope ◽  
A New Species

Abstract Background: The Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae) is an endangered species of mammal endemic to the Qinghai-Tibetan Plateau. Parasites and parasitic diseases are considered to be important threats in the conservation of the Tibetan antelope. However, our present knowledge of the composition of the parasites from the Tibetan antelope remains limited. Methods: Large numbers of nematode parasites were collected from a dead Tibetan antelope. The morphology of these nematode specimens was observed using light and scanning electron microscopy. The nuclear and mitochondrial DNA sequences [i.e. small subunit ribosomal DNA (18S), large subunit ribosomal DNA (28S), internal transcribed spacer (ITS) and cytochrome c oxidase subunit 1 (cox1)] were amplified and sequenced for molecular identification. Moreover, phylogenetic analyses were performed using maximum likelihood (ML) inference based on 28S and 18S + 28S + cox1 sequence data, respectively, in order to clarify the systematic status of these nematodes.Results: Integrated morphological and genetic evidence reveals these nematode specimens to be a new species of pinworm Skrjabinema longicaudatum (Oxyurida: Oxyuridae). There was no intraspecific nucleotide variation between different individuals of S. longicaudatum sp. n. in the partial 18S, 28S, ITS and cox1 sequences. However, a high level of nucleotide divergence was revealed between the new species and its congeners in 28S (8.36%) and ITS (20.3–23.7%) regions, respectively. Molecular phylogenetic results suggest that the genus Skrjabinema should belong to the subfamily Oxyurinae (Oxyuroidea: Oxyuridae), instead of the subfamily Syphaciidae or Skrjabinemiinae in the traditional classification, as it formed a sister relationship to the genus Oxyuris. Conclusions: A new species of pinworm Skrjabinema longicaudatum sp. n. (Oxyurida: Oxyuridae) is described. Skrjabinema longicaudatum sp. n. represents the first species of Oxyurida (pinworm) and the fourth nematode species reported from the Tibetan antelope. Our results contribute to the knowledge of the species diversity of parasites from the Tibetan antelope, and clarify the systematic position of the genus Skrjabinema.

Description of Tylencholaimus discus sp. n. (Nematoda, Dorylaimida, Tylencholaimidae) from Iran

Zootaxa ◽  
2019 ◽  
Vol 4551 (3) ◽  
pp. 379
Author(s):  
BEHROUZ GOLHASAN ◽  
RAMIN HEYDARI ◽  
REYES PEÑA-SANTIAGO
Keyword(s):  
New Species ◽  
Anterior Region ◽  
Genital System ◽  
Neck Length ◽  
Natural Areas ◽  
Posterior Branch ◽  
Body Diameter ◽  
Female Genital System ◽  
A New Species ◽  
Female Genital

A new species of the genus Tylencholaimus, collected in natural areas of Iran, is described and illustrated, including SEM observations. Tylencholaimus discus sp. n. is characterized by its 0.65–0.76 mm long body, lip region offset by weak constriction and 7–7.5 µm broad with a conspicuous perioral disc, odontostyle 6–7 µm long, odontophore 9–10.5 µm long and visibly flanged rather than knobbed, neck 230–259 µm long with anterior region weakly muscular and basal expansion 98–125 µm long or occupying 42–49% of total neck length, female genital system mono-prodelphic, posterior branch reduced to a uterine sac 2–20 µm long or much shorter (0.1–0.6 times) than body diameter but very occasionally consisting of uterus, sphincter and a small terminal mass all together measuring 35–40 µm long, V = 65–74, tail short and rounded (13–17 µm, c = 41–57, c’ = 0.6–0.8), and male unknown. 

scholarly journals Morphology, genetic characterization and molecular phylogeny of pinworm Skrjabinema longicaudatum sp. n. (Oxyurida: Oxyuridae) from the endangered Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae)

2020 ◽  
Author(s):  
Yi-Fan Cao ◽  
Hui-Xia Chen ◽  
Yang Li ◽  
Dang-Wei Zhou ◽  
Shi-Long Chen ◽  
...  
Keyword(s):  
New Species ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Small Subunit ◽  
Sister Relationship ◽  
Tibetan Antelope ◽  
A New Species

Abstract Background: The Tibetan antelope Pantholops hodgsonii (Abel) (Artiodactyla: Bovidae) is an endangered species of mammal endemic to the Qinghai-Tibetan Plateau. Parasites and parasitic diseases are considered to be important threats in the conservation of the Tibetan antelope. However, our present knowledge of the composition of the parasites from the Tibetan antelope remains limited. Methods: Large numbers of nematode parasites were collected from a dead Tibetan antelope. The morphology of these nematode specimens was observed using light and scanning electron microscopy. The nuclear and mitochondrial DNA sequences [i.e. small subunit ribosomal DNA (18S), large subunit ribosomal DNA (28S), internal transcribed spacer (ITS) and cytochrome c oxidase subunit 1 (cox1)] were amplified and sequenced for molecular identification. Moreover, phylogenetic analyses were performed using maximum likelihood (ML) inference based on 28S and 18S + 28S + cox1 sequence data, respectively, in order to clarify the systematic status of these nematodes.Results: Integrated morphological and genetic evidence reveals these nematode specimens to be a new species of pinworm Skrjabinema longicaudatum (Oxyurida: Oxyuridae). There was no intraspecific nucleotide variation between different individuals of S. longicaudatum sp. n. in the partial 18S, 28S, ITS and cox1 sequences. However, a high level of nucleotide divergence was revealed between the new species and its congeners in 28S (8.36%) and ITS (20.3–23.7%) regions, respectively. Molecular phylogenetic results suggest that the genus Skrjabinema should belong to the subfamily Oxyurinae (Oxyuroidea: Oxyuridae), instead of the subfamily Syphaciidae or Skrjabinemiinae in the traditional classification, as it formed a sister relationship to the genus Oxyuris. Conclusions: A new species of pinworm Skrjabinema longicaudatum sp. n. (Oxyurida: Oxyuridae) is described. Skrjabinema longicaudatum sp. n. represents the first species of Oxyurida (pinworm) and the fourth nematode species reported from the Tibetan antelope. Our results contribute to the knowledge of the species diversity of parasites from the Tibetan antelope, and clarify the systematic position of the genus Skrjabinema.

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