Lectin Cytochemistry Reveals Differences between Hamster Trachea and Bronchus in the Composition of Epithelial Surface Glycoconjugates and in the Response of Secretory Cells to Neutrophil Elastase

1990 ◽  
Vol 3 (1) ◽  
pp. 61-69 ◽  
Author(s):  
Thomas G. Christensen ◽  
Raphael Breuer ◽  
Edgar C. Lucey ◽  
Linda J. Hornstra ◽  
Phillip J. Stone ◽  
...  
Keyword(s):  
Neutrophil Elastase ◽  
Secretory Cells ◽  
Epithelial Surface ◽  
Lectin Cytochemistry ◽  
Hamster Trachea

scholarly journals Histochemical structure and immunolocalisation of the hyaluronan system in the dromedary oviduct

2016 ◽  
Vol 28 (7) ◽  
pp. 936 ◽  
Author(s):  
Omnia Mohey-Elsaeed ◽  
Waleed F. A. Marei ◽  
Ali A. Fouladi-Nashta ◽  
Abdel-Aleem A. El-Saba
Keyword(s):  
Epithelial Cells ◽  
Molecular Size ◽  
Blue Staining ◽  
Secretory Cells ◽  
Alcian Blue Staining ◽  
Basal Region ◽  
Epithelial Surface ◽  
Ciliated Epithelial Cells ◽  
Oviductal Fluid ◽  
Local Modulation

We investigated the local modulation of some histochemical properties of oviducts of the dromedary (Camelus dromedarius), focusing on the immnolocalisation of hyaluronic acid (HA) synthases (HAS2 and HAS3), hyaluronidases (HYAL2 and HYAL1) and the HA receptor CD44 in the ampulla and isthmus. Abundant acidic mucopolysaccharides (glycosaminoglycans) were detected by Alcian blue staining along the luminal surface of both ciliated and non-ciliated epithelial cells (LE). Staining for HAS2 was higher in the primary epithelial folds of the ampulla compared with the isthmus, especially in secretory cells, adluminal epithelial surface and supranuclear cell domain. HAS3 staining was stronger in the LE of the isthmus than ampulla. HYAL2 was detected in the LE in the ampulla and isthmus and was more intense in the adluminal projections of secretory cells. HYAL1 was weakly detected in the LE with no difference between the ampulla and isthmus. Strong CD44 immunostaining was present in the LE of the ampulla and isthmus. CD44 staining was higher in secretory cells than in ciliated epithelial cells and was higher in the supranuclear region than the basal region of the cytoplasm. In conclusion, we provide evidence that HA synthesis and turnover occur in the camel oviduct. Differences in HAS2 and HAS3 expression suggest regional differences in the molecular size of HA secreted in oviductal fluid that may influence oviduct–gamete interaction in the camel.

scholarly journals HISTOLOGICAL VARIATIONS IN FALLOPIAN TUBE DURING VARIOUS PHASES OF OESTRUS CYCLE

2018 ◽  
Vol 26 (1) ◽  
Author(s):  
Virendra Kumar ◽  
Aditi Srivastava
Keyword(s):  
Fallopian Tube ◽  
Reproductive System ◽  
Secretory Cells ◽  
Fallopian Tubes ◽  
Epithelial Surface ◽  
Histological Changes ◽  
Oestrus Cycle ◽  
Vital Functions ◽  
Vascular Activity ◽  
Products Of Conception

Introduction: The fallopian tube is the vital part of reproductive system where the fertilization of ova takes place. It also acts as a transporting channel for passing the ova and the products of conception to the uterus. To perform these vital functions fallopian tubes u ndergo various histological va riations during va rious phases of oestrus cycle. Material & Methods: Histology of fallopian tubes of 50 female rabbits were studied by using H & E staining. Study of the cytology of vagina of the animal decided the phase of the oestrus cycle i.e. proestrus, oestrus, metaestrus and diestrus phases by Papaniculou's staining. Observations and Results: The study revealed histological changes in relation to various phases of oestrus cycle. Epithelial surface area was maximum in oestrus phase. Secretory cells were most active in metaestrus and diestrus phases. Cilliary cells were prominent in proestrus and oestrus phases. The vascular activity was found maxim um in oestrus phase

scholarly journals Histology of the light organs of Pholas dactylus (Lamellibranchia)

1960 ◽  
Vol 39 (1) ◽  
pp. 109-115 ◽  
Author(s):  
J. A. C. Nicol
Keyword(s):  
Outer Part ◽  
Mantle Cavity ◽  
Surface Epithelium ◽  
Secretory Cells ◽  
Epithelial Surface ◽  
Secretory Cycle ◽  
Light Organs ◽  
Glandular Cells ◽  
Mucus Cells ◽  
Pyriform Cells

The piddock, Pholas dactylus L., gives off a luminous secretion when irritated. The luminous glands which produce the secretion are two longitudinal stripes in the exhalant siphon, a pair of triangular organs in the mantle cavity near the base of the siphon, and a stripe around the ventral rim of the mantle (Panceri, 1872).The histology of the light-organs has been described several times. A light-organ is covered by a simple columnar ciliated epithelium, below which are many glandular cells, which discharge through the surface epithelium. The outer part of the glandular layer consists of a mass of large mucus cells. Deeper lies a second glandular region containing large cells with long necks that extend to the external epithelial surface. Dubois (1892, 1914, 1928) believed that the photogenic tissue was made up of two kinds of secretory cells; these were the superficial ciliated cells, which possessed glandular bases (fixed secretory cells); and deeper lying glandular cells derived from clasmatocytes (migratory secretory cells). Rawitz (1891) clearly distinguished a mucous from an underlying photogenic layer. The latter, according to Förster (1914), contains pyriform cells with long necks. He believed that he could distinguish a secretory cycle in the photogenic cells. Exhausted cells at the beginning of the cycle possessed an alveolar cytoplasm; granules began to appear in the cytoplasm; the granules increased in number and stained intensely with iron haematoxylin. Those photogenic cells which were filled with granules were in the active secretory state. Transitional stages between the inactive (or depleted) cells and the active (granular) cells were rare.

Further Observations on the Secretory Cells in the Optic Tentacles of Helix, with Special Reference to the Results of Vital Staining

1964 ◽  
Vol s3-105 (69) ◽  
pp. 61-71
Author(s):  
NANCY J. LANE
Keyword(s):  
Special Reference ◽  
Calcium Salt ◽  
Vital Staining ◽  
Neutral Red ◽  
Nuclear Bodies ◽  
Secretory Cells ◽  
Oval Cells ◽  
Epithelial Surface ◽  
Janus Green ◽  
Inner Surface

When the collar cells or the lateral cells in the tentacles of Helix aspersa are stained with neutral red and other vital dyes, uptake of the dyes occurs chiefly in the lipoidal β-bodies. Janus green B and dahlia are taken up by the mitochondria. In vitally-coloured preparations, no ‘peri-nuclear bodies’ are present in the collar and lateral oval cells, such as were previously found in these cells after post-osmication for 14 days. Fixed preparations of similar cells, post-osmicated for different periods, indicate that the peri-nuclear dictyosomes are artifacts due to over-impregnation with osmium. In addition to the lateral oval and lateral processed cells, which lie at intervals along the inner surface of the dermo-muscular sheath in Helix, unicellular calcium glands are found, lying immediately beneath the epithelial surface; these are far more numerous in H. pomatia than in H. aspersa. They contain granules of a calcium salt suspended in a matrix of mucopolysaccharide, and apparently produce the whitish mucoid secretion that is extruded on to the surface of the tentacles when the animal is disturbed. There is no evidence to suggest that the lateral cells are concerned in the production of this mucoid secretion.

scholarly journals Anti-neutrophil elastase defense of the normal human respiratory epithelial surface provided by the secretory leukoprotease inhibitor.

1991 ◽  
Vol 87 (2) ◽  
pp. 482-488 ◽  
Author(s):  
C Vogelmeier ◽  
R C Hubbard ◽  
G A Fells ◽  
H P Schnebli ◽  
R C Thompson ◽  
...  
Keyword(s):  
Neutrophil Elastase ◽  
Epithelial Surface ◽  
Normal Human ◽  
Respiratory Epithelial

Resistance of Hamster Bronchiolar Epithelium to Neutrophil Elastase: Investigation by Cell Surface Lectin Cytochemistry

1992 ◽  
Vol 18 (1) ◽  
pp. 115-129 ◽  
Author(s):  
Thomas G. Christensen ◽  
Raphael Breuer ◽  
Christopher E. Haddad ◽  
Edgar C. Lucey ◽  
Phillip J. Stone ◽  
...  
Keyword(s):  
Cell Surface ◽  
Neutrophil Elastase ◽  
Lectin Cytochemistry ◽  
Bronchiolar Epithelium

scholarly journals Histochemical methods for characterizing secretory and cell surface sialoglycoconjugates.

1985 ◽  
Vol 33 (5) ◽  
pp. 427-438 ◽  
Author(s):  
B A Schulte ◽  
S S Spicer
Keyword(s):  
Sialic Acid ◽  
Periodate Oxidation ◽  
Acinar Cells ◽  
Pancreatic Acinar Cells ◽  
Surface Epithelium ◽  
Secretory Cells ◽  
Sublingual Gland ◽  
Mucous Cells ◽  
Acetylneuraminic Acid ◽  
Hamster Trachea

Paraffin sections of trachea, sublingual gland, and pancreas from rats, mice, and hamsters were stained with peanut agglutinin (PNA) or Dolichos biflorus agglutinin (DBA) conjugated to horseradish peroxidase before or after enzymatic removal of sialic acid. Adjacent sections were oxidized with periodate prior to incubation with sialidase and staining with PNA and DBA. PNA binding demonstrated terminal beta-galactose in secretions, at the basolateral plasmalemma of mouse tracheal serous cells, in or at the surface of zymogen granules, and at the apical and basolateral surface of mouse and hamster pancreatic acinar cells. Sialidase digestion revealed PNA binding, demonstrative of penultimate beta-galactose, in secretions of mucous cells in tracheal and sublingual glands and at the apical glycocalyx of ciliated and secretory cells in the tracheal surface epithelium of all the rodents studied. Sialidase also imparted PNA affinity to endothelium in all three species and to secretions and the basolateral plasmalemma of tracheal serous cells and pancreatic acinar cells in the rat. Periodate oxidation blocked the enzymatic removal of N-acetylneuraminic acid as judged by prevention of staining with the sialidase-PNA procedure. Sites in which periodate prevented sialidase-PNA staining included pancreatic islet cells and at the luminal glycocalyx of ciliated and secretory cells in tracheal surface epithelium in all three rodents, most sublingual mucous cells in the hamster, pancreatic acinar cells in the rat, and endothelium, except that of the rat. Glycoconjugate in other sites remained positive with the periodate-sialidase-PNA sequence. Resistance to periodate was interpreted as evidence for the presence of terminal sialic acid with an O-acetylated polyhydroxyl side chain. DBA binding demonstrated terminal alpha-N-acetylgalactosamine in the secretion of all mucous cells in the hamster trachea and 50-90% of those in the rat, secretion and the basolateral plasmalemma of all glandular serous cells in the mouse trachea, at the apical surface of most secretory cells lining the lumen of the rat and hamster trachea, and cilia of 5-10% of ciliated cells in the rat trachea. Periodate oxidation and sialidase digestion demonstrated N-acetylneuraminic acid and penultimate alpha-N-acetylgalactosamine in cilia in the mouse trachea and sialic acid containing O-acetylated polyhydroxyl side chains subtended by N-acetylgalactosamine in the secretion of all mucous cells in the rat and hamster trachea and of 80-90% of mucous cells in the hamster sublingual gland.(ABSTRACT TRUNCATED AT 400 WORDS)

The ultrastructure of acinar cells in the external orbital gland of young and old male rats

1978 ◽  
Vol 36 (2) ◽  
pp. 276-277
Author(s):  
R. Carriere
Keyword(s):  
Young Adulthood ◽  
Acinar Cells ◽  
Cell Number ◽  
Male Rats ◽  
Polyploid Cell ◽  
Secretory Cells ◽  
Age Changes ◽  
Albino Rat ◽  
Golgi Membranes ◽  
Diploid Cells

The external orbital gland of the albino rat exhibits both sexual dimorphism and histological age changes. In males, many cells attain a remarkable degree of polyploidy and an increase of polyploid cell number constitutes the major age change until young adulthood. The acini of young adults have a small lumen and are composed of tall serous cells. Subsequently, many acini acquire a larger lumen with an irregular outline while numerous vacuoles accumulate throughout the secretory cells. At the same time, vesicular acini with a large lumen surrounded by pale-staining low cuboidal diploid cells begin to appear and their number increases throughout old age. The fine structure of external orbital glands from both sexes has been explored and in considering acinar cells from males, emphasis was given to the form of the Golgi membranes and to nuclear infoldings of cytoplasmic constituents.

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