Disposable Amperometric Immunosensor for Hepatitis B Antigen Detection Based on Multiwalled Carbon Nanotubes and Ferrocene Decorated Screen Printed Electrode

2019 ◽  
Vol 15 (5) ◽  
pp. 930-938
Author(s):  
Zhencheng Chen ◽  
Yan Bai ◽  
Feijun Zhao ◽  
Liangli Cao ◽  
Guocheng Han ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Hepatitis B ◽  
Multiwalled Carbon Nanotubes ◽  
Antigen Detection ◽  
Screen Printed Electrode ◽  
Multiwalled Carbon ◽  
Amperometric Immunosensor ◽  
Hepatitis B Antigen ◽  
Screen Printed

scholarly journals Disposable Screen-Printed Electrode Coupled with Recombinant Drosophila melanogaster Acetylcholinesterase and Multiwalled Carbon Nanotubes for Rapid Detection of Pesticides

2011 ◽  
Vol 94 (1) ◽  
pp. 307-312 ◽  
Author(s):  
Zhuzhao Tang ◽  
Haode Chen ◽  
Shiping Song ◽  
Chunhai Fan ◽  
Dabing Zhang ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Drosophila Melanogaster ◽  
Multiwalled Carbon Nanotubes ◽  
Water Samples ◽  
Rapid Detection ◽  
Sea Water ◽  
Screen Printed Electrode ◽  
Multiwalled Carbon ◽  
Alternative Means ◽  
Screen Printed

Abstract Recombinant Drosophila melanogaster acetylcholinesterase (R-DmAChE), multiwalled carbon nanotubes (MWCNTs), and Prussian blue have been combined for development of a three-electrode biosensor with more rapid responses and higher stability than in our previous study. A new disposable screen-printed electrode (SPE) was developed for rapid detection of organophosphate and carbamate pesticides. After optimization, 10 μg MWCNT and 5 μL enzyme immobilization solution consisting of 0.2% glutaraldehyde, 0.1% Nafion®, 0.2% bovine serum albumin, 0.1 g/L MWCNT, and 1.5 mU R-DmAChE were fixed on each of the R-DmAChE/MWCNT SPEs. The LOD of this biosensor was 0.5 μg/L for pesticide standards of dichlorvos (DDV) and carbofuran. The performance of this biosensor was tested for vegetable and water samples at various spiked levels, and good stability and sensitivity were found. The obtained recoveries were from 82.6 to 110.5% for DDV at levels of 0.5–5 μg/L and 73.4 to 118.4% for carbofuran at 1–10 μg/L in lake and sea water samples, demonstrating that the proposed approach is an alternative means for rapid detection of pesticide residues and contaminants in food safety and environmental monitoring.

scholarly journals First Electrochemical Sensor (Screen-Printed Carbon Electrode Modified with Carboxyl Functionalized Multiwalled Carbon Nanotubes) for Ultratrace Determination of Diclofenac

Materials ◽  
2020 ◽  
Vol 13 (3) ◽  
pp. 781 ◽  
Author(s):  
Agnieszka Sasal ◽  
Katarzyna Tyszczuk-Rotko ◽  
Magdalena Wójciak ◽  
Ireneusz Sowa
Keyword(s):  
Carbon Nanotubes ◽  
Electrochemical Sensor ◽  
Multiwalled Carbon Nanotubes ◽  
Active Surface Area ◽  
Carbon Electrode ◽  
Multiwalled Carbon ◽  
Screen Printed Carbon Electrode ◽  
Functionalized Multiwalled Carbon Nanotubes ◽  
Screen Printed

A simple, sensitive and time-saving differential-pulse adsorptive stripping voltammetric (DPAdSV) procedure using a screen-printed carbon electrode modified with carboxyl functionalized multiwalled carbon nanotubes (SPCE/MWCNTs-COOH) for the determination of diclofenac (DF) is presented. The sensor was characterized using optical profilometry, SEM, and cyclic voltammetry (CV). The use of carboxyl functionalized MWCNTs as a SPCE modifier improved the electron transfer process and the active surface area of sensor. Under optimum conditions, very sensitive results were obtained with a linear range of 0.1–10.0 nmol L−1 and a limit of detection value of 0.028 nmol L−1. The SPCE/MWCNTs-COOH also exhibited satisfactory repeatability, reproducibility, and selectivity towards potential interferences. Moreover, for the first time, the electrochemical sensor allows determining the real concentrations of DF in environmental water samples without sample pretreatment steps.

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