The Influence of Growth Regulators on In-Vitro Culture of Rosa Hybrida

2019 ◽  
Vol 16 (7) ◽  
pp. 2990-2994
Author(s):  
Kazem Kamali Aliabad ◽  
Amir Rezaee ◽  
Farhad Homayoonfar ◽  
Elahe Zamani
Keyword(s):  
In Vitro Culture ◽  
Growth Regulators ◽  
Rosa Hybrida

scholarly journals Introduction to in vitro culture and callus initiation in Salvia hispanica L. (chia)

2019 ◽  
Vol 17 (1) ◽  
pp. 33-37
Author(s):  
A. Z. Revutskaya ◽  
A. V. Holubenko ◽  
N. V. Nuzhyna ◽  
H. O. Rudik ◽  
N. Yu. Taran
Keyword(s):  
In Vitro Culture ◽  
Growth Regulators ◽  
Nutrient Medium ◽  
Callus Growth ◽  
Basic Medium ◽  
High Concentration ◽  
Salvia Hispanica ◽  
Callus Initiation ◽  
Salvia Hispanica L

Aim. Preparation of aseptic seedlings Salvia hispanica L., callus initiation in vitro and establishment of primary explants suitable for the callus production. Methods. Seeds are sprouted on our own modification of conventional methods. The non-hormonal Murashige-Skoog agarized nutrient medium was used as basic medium for the experiments. Parts of one-month seedlings (roots, hypocotyl, cotyledon leaves) were used as explants for the use of the colza. We added growth regulators (BAP, 2,4-D) in different concentration combinations into the nutrient medium for callus initiation. Statistical processing was performed in Microsoft Office Excel. Results. Aseptic S. hispanica seedlings have been obtained. The callus growth was initiated on all types of explants, the dependence of the callus intensity on the type of explants and the growth regulators content in the nutrient medium was established. Morphogenic callus and root-regenerants have been obtained. Conclusions. Hypocotyl was the most suitable primary explant for callus growth. Seedlings, leaves and roots showed low morphogenetic capacity. The nutrient medium with an elevated 2,4-D content was the most effective for initiation of callus genesis and proliferation of non-morphogenous callus. A high concentration of 2,4-D in the medium improves S. hispanica callus growth but suppresses its morphogenic ability.Keywords: Salvia hispanica (Chia), in vitro culture, callus.

scholarly journals Growth regulators and darkness increase efficiency in in vitro culture of immature embryos from peppers

2014 ◽  
Vol 71 (6) ◽  
pp. 488-493 ◽  
Author(s):  
Juan Pablo Manzur ◽  
María de las Nieves Calvache-Asensio ◽  
Adrian Rodriguez-Burruezo
Keyword(s):  
In Vitro Culture ◽  
Growth Regulators ◽  
Immature Embryos ◽  
Increase Efficiency

scholarly journals Some preliminary results of experiments with in-vitro culture of dipterocarps.

1983 ◽  
Vol 31 (3) ◽  
pp. 233-238
Author(s):  
W.T.M. Smits ◽  
B. Struycken
Keyword(s):  
In Vitro Culture ◽  
Growth Regulators ◽  
Leaf Explants ◽  
Axillary Buds ◽  
Parent Plant ◽  
Preliminary Results ◽  
Murashige And Skoog Medium ◽  
Half Strength ◽  
Strength Medium

A study was made of the influence of light, temp., growth regulators, and sugar and macrosalt concn. on the growth and morphogenesis of leaf and axis explants of young Shorea curtisii, S. obtusa and Dipterocarpus grandiflorus. Terminal and axillary buds grew best on half strength Murashige and Skoog medium. Leaf explants formed more callus on full strength medium, when containing part of the midrib and when taken from the lower half of the leaf. More than 95% of D. grandiflorus explants were infected by a fungus apparently present in the parent plant. (Abstract retrieved from CAB Abstracts by CABI’s permission)

scholarly journals The Effect of Plant Growth Regulators on Shoot Proliferation and Rooting of Crataegus Pseudohetrophylla Pojark. Via in Vitro Culture

2015 ◽  
Vol 5 (17) ◽  
pp. 85-95
Author(s):  
F. Ahmadloo ◽  
M. Tabari Kouchaksaraei ◽  
P. Azadi ◽  
A. Hamidi ◽  
E. Beiramizadeh ◽  
...  
Keyword(s):  
Plant Growth ◽  
In Vitro Culture ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Shoot Proliferation

scholarly journals In vitro morphogenesis of different mangabeira (Hancornia speciosa gomes) varieties from the savanna of the Goiás

2018 ◽  
Vol 11 (5) ◽  
pp. 82-88
Author(s):  
M. C. Vieira ◽  
R. M. Oliviera ◽  
J. B. A. França ◽  
C. C. P. Oliveira ◽  
E. R. B. Souza
Keyword(s):  
In Vitro Culture ◽  
Growth Regulators ◽  
Genetic Improvement ◽  
Fruit Tree ◽  
The Other ◽  
In Vitro Germination ◽  
In Vitro Morphogenesis ◽  
Federal Institute ◽  
Hancornia Speciosa

Hancornia speciosa is a fruit tree that is in the process of domestication. This time the propagation in vitro when well developed can promote conditions for the production of quality seedlings for this fruit. In vitro culture technologies are of great importance for conservation programs of genetic resources and genetic improvement of mangabeira. The objective of this study is to establish a methodology the micropropagation of Hancornia speciosa, Gomes, of three varieties (cuyabensis, gardneri and pubescens) by in vitro germination of seeds under different growth regulators. Fruits were collected at School of Agronomy of the Federal University of Goiás, transported to the Laboratory of Biotechnology, Federal Institute Campus Urutaí - Goiás, inoculated for germination in test tubes containing MS growth medium with 50% of salts concentrations, with five different concentrations of IBA. It was observed that the in vitro germination of cuyabensis, gardneri, and pubescens were 66.00 %, 64.05 % and 76 %, respectively. The beginning of germination occurred from three days for gardneri variety, six days for pubescens variety, and eigh day for cuyabensis variety, extending to the 16 days for gardneri variety, and 28 days for the other varieties. Explants of different varieties differ in their manifestations when cultured in vitro. The aseptic method was effective for the control of fungal agents

scholarly journals EFFICIENT in vitro PROPAGATION OF Amaranthus viridis L. USING NODE EXPLANTS

2020 ◽  
Vol 19 (4) ◽  
pp. 41-51
Author(s):  
Tour Jan ◽  
Ikram Ullah ◽  
Bilal Muhammad ◽  
_ Tariq ◽  
Ali Mansoor ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Ammonium Nitrate ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Dark Green

Hyperhydricity is a frequently problem in plants during in vitro culture and affected micropropagation ofplants. To develop an efficient in vitro regenerated system without hyperdydricity, we demonstrated the effectof different disinfected agents (mercuric chlorite and hypochlorite), growth regulators, their concentrationsand combinations, Agar, pH, ammonium nitrate (NH4NO3) and number of subcultures. Mercuric chlorite at0.07% and exposing time (9–10 min) was appropriate for hygienic culture. The shoots induced by Benzyladnine(BA) alone or in combination with α-Naphthaleneacetic acid (NAA) exhibited maximum multiplicationwith symptoms of hyperhydricity than those induced by Kinetin alone or in combination with NAA. Hyperhydricitywas also reduced by increasing the concentration of agar, pH and elimination of NH4NO3 from themacroelements of Murashig and Skoog (MS) medium. Repeated subcultures affected both multiplication andhyperhydricity. The multiplication of shoots increased from parental culture up to 5th subculture and thereafterdeclined in 6th subculture. Although shoot hyperhydricity were observed from 1st subculture (19%) andthen increased up to 85% in 6th subculture. This increased in hyperhydricity could be due to the remaininginfluence of hormones. In shoots of 5th subculture the content of chlorophyll (dark green) were higher thanshoots of 6th subculture.

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