Engineering an alginate/β-glycerophosphate/dextran injectable hydrogel-delivery for cardiac therapies after acute myocardial infarctions

2021 ◽  
Vol 11 (6) ◽  
pp. 846-853
Author(s):  
Deping Meng ◽  
Chunmei Diao ◽  
Liyan Liang
Keyword(s):  
Stem Cell ◽  
Human Mesenchymal Stem Cell ◽  
Myocardial Infarctions ◽  
Survival Ratio ◽  
Synergic Effect ◽  
Injectable Hydrogel ◽  
Mesenchymal Stem Cell Therapy ◽  
Myocardial Infraction ◽  
Regenerative Activity

Human mesenchymal stem cell therapy (hMSCs) enables the transmission of alginate (A)/dextran (D)/β- glycerophosphate (β-GP) to the human myocardials infarcts. The fabricated hMSCs-AD@β-GP exhibited pixelation rate, pore size, absorbency, and aggregation rate values of 65.3%, 146.2%, 92.0%, and 29.1% with 1%, respectively. The hMSCs-AD@β-GP produced were physiochemically examined and were found to be highly stable, verifying the usefulness of the nanomaterials for cardiac regenerations. The in vitro studies of hMSCs- AD@β-GP hydrogels have indicated that the cell survival ratio improved, and the positive findings of ejection fraction, fibrosis, and vessel density with lowered infraction size indicated that the heart regenerative activity also improved significantly after myocardial infraction. The synergic effect of the hydrogel with hMSCs-AD@β- GP may, therefore, be suitable to treat patients with severe myocardial infarctions.

Biomaterials Nano Geometry for Control of Stem Cell Differentiation

2009 ◽  
Vol 1239 ◽  
Author(s):  
Karla Brammer ◽  
Seunghan Oh ◽  
Sungho Jin
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Stem Cell Differentiation ◽  
Human Mesenchymal Stem Cell ◽  
Oxide Surface ◽  
Specific Cell ◽  
Implant Surface ◽  
Multipotent Stem Cells

AbstractTwo important goals in stem cell research are to control the cell proliferation without differentiation, and also to direct the differentiation into a specific cell lineage when desired. Recent studies indicate that the nanostructures substantially influence the stem cell behavior. It is well known that mesenchymal stem cells (MSCs) are multipotent stem cells that can differentiate into stromal lineages such as adipocyte, chondrocyte, fibroblast, myocyte, and osteoblast cell types. By examining the cellular behavior of MSCs cultured in vitro on nanostructures, some understanding of the effects that the nanostructures have on the stem cell’s response has been obtained. Here we demonstrate that TiO2 nanotubes produced by anodization on Ti implant surface can regulate human mesenchymal stem cell (hMSC) differentiation towards an osteoblast lineage in the absence of osteogenic inducing factors. Altering the dimensions of nanotubular-shaped titanium oxide surface structures independently allowed either augmented human mesenchymal stem cell (hMSC) adhesion at smaller diameter levels or a specific differentiation of hMSCs into osteoblasts using only the geometric cues. Small (˜30 nm diameter) nanotubes promoted adhesion without noticeable differentiation, while larger (˜70 - 100 nm diameter) nanotubes elicited a dramatic, ˜10 fold stem cell elongation, which induced cytoskeletal stress and selective differentiation into osteoblast-like cells, offering a promising nanotechnology-based route for novel orthopaedics-related hMSC treatments. The fact that a guided and preferential osteogenic differentiation of stem cells can be achieved using substrate nanotopography alone without using potentially toxic, differentiation-inducing chemical agents is significant, which can be useful for future development of novel and enhanced stem cell control and therapeutic implant development.

scholarly journals Transcriptomic Analysis of Human Mesenchymal Stem Cell Therapy in Incontinent Rat Injured Urethra

2020 ◽  
Vol 26 (13-14) ◽  
pp. 792-810
Author(s):  
Zhina Sadeghi ◽  
Jonathan D. Kenyon ◽  
Brian Richardson ◽  
Ahmad O. Khalifa ◽  
Michael Cartwright ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Therapy ◽  
Stem Cell Therapy ◽  
Human Mesenchymal Stem Cell ◽  
Transcriptomic Analysis ◽  
Mesenchymal Stem Cell Therapy

scholarly journals Regulation of JAM2 Expression in the Lungs of Streptozotocin-Induced Diabetic Mice and Human Pluripotent Stem Cell-Derived Alveolar Organoids

Biomedicines ◽  
2020 ◽  
Vol 8 (9) ◽  
pp. 346 ◽  
Author(s):  
Roya Rasaei ◽  
Eunbi Kim ◽  
Ji-Young Kim ◽  
Sunghun Na ◽  
Jung-Hyun Kim ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Causative Factor ◽  
Human Mesenchymal Stem Cell ◽  
Therapeutic Effects ◽  
Diabetic Mice ◽  
Human Pluripotent Stem Cell ◽  
Potential Indicator

Hyperglycemia is a causative factor in the pathogenesis of respiratory diseases, known to induce fibrosis and inflammation in the lung. However, little attention has been paid to genes related to hyperglycemic-induced lung alterations and stem cell applications for therapeutic use. In this study, our microarray data revealed significantly increased levels of junctional adhesion molecule 2 (JAM2) in the high glucose (HG)-induced transcriptional profile in human perivascular cells (hPVCs). The elevated level of JAM2 in HG-treated hPVCs was transcriptionally and epigenetically reversible when HG treatment was removed. We further investigated the expression of JAM2 using in vivo and in vitro hyperglycemic models. Our results showed significant upregulation of JAM2 in the lungs of streptozotocin (STZ)-induced diabetic mice, which was greatly suppressed by the administration of conditioned medium obtained from human mesenchymal stem cell cultures. Furthermore, JAM2 was found to be significantly upregulated in human pluripotent stem cell-derived multicellular alveolar organoids by exposure to HG. Our results suggest that JAM2 may play an important role in STZ-induced lung alterations and could be a potential indicator for predicting the therapeutic effects of stem cells and drugs in diabetic lung complications.

Effects of Hydrostatic Pressure and Transforming Growth Factor-β3 on Adult Human Mesenchymal Stem Cell Chondrogenesis In Vitro

2006 ◽  
Vol 12 (6) ◽  
pp. 1419-1428 ◽  
Author(s):  
Keita Miyanishi ◽  
Michael C.D. Trindade ◽  
Derek P. Lindsey ◽  
Gary S. Beaupré ◽  
Dennis R. Carter ◽  
...  
Keyword(s):  
Stem Cell ◽  
Growth Factor ◽  
Hydrostatic Pressure ◽  
Mesenchymal Stem Cell ◽  
Transforming Growth Factor ◽  
Human Mesenchymal Stem Cell ◽  
Adult Human
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