Aldose Reductase Deficiency Reduced Vascular Changes in Neonatal Mouse Retina in Oxygen-Induced Retinopathy

2012 ◽  
Vol 53 (9) ◽  
pp. 5698 ◽  
Author(s):  
Zhong Jie Fu ◽  
Suk-Yee Li ◽  
Norbert Kociok ◽  
David Wong ◽  
Sookja K. Chung ◽  
...  
Keyword(s):  
Aldose Reductase ◽  
Neonatal Mouse ◽  
Mouse Retina ◽  
Vascular Changes ◽  
Reductase Deficiency ◽  
Oxygen Induced Retinopathy

scholarly journals Aldose reductase deficiency protects sugar-induced lens opacification in rats

2011 ◽  
Vol 191 (1-3) ◽  
pp. 346-350 ◽  
Author(s):  
Aramati B.M. Reddy ◽  
Ravinder Tammali ◽  
Rakesh Mishra ◽  
Shriram Srivastava ◽  
Satish K. Srivastava ◽  
...  
Keyword(s):  
Aldose Reductase ◽  
Reductase Deficiency ◽  
Lens Opacification

Impaired pericyte recruitment and abnormal retinal angiogenesis as a result of angiopoietin-2 overexpression

2007 ◽  
Vol 97 (01) ◽  
pp. 99-108 ◽  
Author(s):  
Yuxi Feng ◽  
Franziska vom Hagen ◽  
Frederick Pfister ◽  
Snezana Djokic ◽  
Sigrid Hoffmann ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Transgenic Animals ◽  
Mouse Retina ◽  
Vascular Density ◽  
Wild Type ◽  
Transgenic Mouse Line ◽  
Oxygen Induced Retinopathy ◽  
Genes Encoding ◽  
Angiopoietin 2 ◽  
The Impact

SummaryAngiopoietin-2 (Ang2) is among the relevant growth factors induced by hypoxia and plays an important role in the initiation of retinal neovascularizations. Ang2 is also involved in incipient diabetic retinopathy, as it may cause pericyte loss. To investigate the impact of Ang2 on developmental and hypoxia-induced angiogenesis, we used a transgenic mouse line overexpressing human Ang2 in the mouse retina. Transgenic mice displayed a reduced coverage of capillaries with pericytes (-14 %; p<0.01) and a 46% increase of vascular density of the capillary network at postnatal day 10 compared to wild type mice. In the model of oxygen-induced retinopathy (OIR), Ang2 overexpression resulted in enhanced preretinal (+103%) and intraretinal neovascularization (+29%). Newly formed intraretinal vessels in OIR were also pericyte-deficient (-26 %; p<0.01). The total expression of Ang2 in transgenic mice was seven-fold, compared with wild type controls. Ang2 modulated expression of genes encoding VEGF (+65%) and Ang1 (+79%) in transgenic animals. These data suggest that Ang2 is involved in pericyte recruitment, and modulates intraretinal, and preretinal vessel formation in the eye under physiological and pathological conditions.

scholarly journals Imaging Retinal Vascular Changes in the Mouse Model of Oxygen-Induced Retinopathy

2012 ◽  
Vol 1 (2) ◽  
pp. 5 ◽  
Author(s):  
João M. Furtado ◽  
Michael H. Davies ◽  
Dongseok Choi ◽  
Andreas K. Lauer ◽  
Binoy Appukuttan ◽  
...  
Keyword(s):  
Mouse Model ◽  
Vascular Changes ◽  
Oxygen Induced Retinopathy

Deep Learning–Based Detection of Endothelial Tip Cells in the Oxygen-Induced Retinopathy Model

2020 ◽  
pp. 019262332097296
Author(s):  
Igor Zingman ◽  
Nina Zippel ◽  
Gerald Birk ◽  
Sebastian Eder ◽  
Leo Thomas ◽  
...  
Keyword(s):  
Data Augmentation ◽  
Neuronal Damage ◽  
Mouse Retina ◽  
Cell Detection ◽  
Single Shot ◽  
Training Procedure ◽  
Oxygen Induced Retinopathy ◽  
Detection Of Objects ◽  
Proliferative Retinopathies ◽  
Tip Cells

Proliferative retinopathies, such as diabetic retinopathy and retinopathy of prematurity, are leading causes of vision impairment. A common feature is a loss of retinal capillary vessels resulting in hypoxia and neuronal damage. The oxygen-induced retinopathy model is widely used to study revascularization of an ischemic area in the mouse retina. The presence of endothelial tip cells indicates vascular recovery; however, their quantification relies on manual counting in microscopy images of retinal flat mount preparations. Recent advances in deep neural networks (DNNs) allow the automation of such tasks. We demonstrate a workflow for detection of tip cells in retinal images using the DNN-based Single Shot Detector (SSD). The SSD was designed for detection of objects in natural images. We adapt the SSD architecture and training procedure to the tip cell detection task and retrain the DNN using labeled tip cells in images of fluorescently stained retina flat mounts. Transferring knowledge from the pretrained DNN and extensive data augmentation reduced the amount of required labeled data. Our system shows a performance comparable to the human level, while providing highly consistent results. Therefore, such a system can automate counting of tip cells, a readout frequently used in retinopathy research, thereby reducing routine work for biomedical experts.

scholarly journals Tropisms of AAV for Subretinal Delivery to the Neonatal Mouse Retina and Its Application for In Vivo Rescue of Developmental Photoreceptor Disorders

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e54146 ◽  
Author(s):  
Satoshi Watanabe ◽  
Rikako Sanuki ◽  
Shinji Ueno ◽  
Toshiyuki Koyasu ◽  
Toshiaki Hasegawa ◽  
...  
Keyword(s):  
Neonatal Mouse ◽  
Mouse Retina

scholarly journals Angiopoietin-2 Deficiency Decelerates Age-Dependent Vascular Changes in the Mouse Retina

2008 ◽  
Vol 21 (1-3) ◽  
pp. 129-136 ◽  
Author(s):  
Yuxi Feng ◽  
Frederick Pfister ◽  
Kay Schreiter ◽  
Yumei Wang ◽  
Oliver Stock ◽  
...  
Keyword(s):  
Mouse Retina ◽  
Vascular Changes ◽  
Age Dependent ◽  
Angiopoietin 2

A reliable method for organ culture of neonatal mouse retina with long-term survival

1999 ◽  
Vol 87 (1) ◽  
pp. 57-65 ◽  
Author(s):  
Judith Mosinger Ogilvie ◽  
Judith D. Speck ◽  
Jaclynn M. Lett ◽  
Timothy T. Fleming
Keyword(s):  
Organ Culture ◽  
Reliable Method ◽  
Neonatal Mouse ◽  
Mouse Retina ◽  
Term Survival ◽  
Long Term Survival
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