scholarly journals Functional characterization of the biogenic amine transporters on human macrophages

JCI Insight ◽  
2022 ◽  
Author(s):  
Phillip M. Mackie ◽  
Adithya Gopinath ◽  
Dominic M. Montas ◽  
Alyssa Nielsen ◽  
Aidan Smith ◽  
...  
Keyword(s):  
Biogenic Amine ◽  
Functional Characterization ◽  
Human Macrophages ◽  
Biogenic Amine Transporters

Transcriptional regulation and functional characterization of the oxysterol/EBI2 system in primary human macrophages

2014 ◽  
Vol 446 (3) ◽  
pp. 663-668 ◽  
Author(s):  
Inga Preuss ◽  
Marie-Gabrielle Ludwig ◽  
Birgit Baumgarten ◽  
Frederic Bassilana ◽  
Francois Gessier ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Functional Characterization ◽  
Human Macrophages

Studies of the biogenic amine transporters. VII. Characterization of a novel cocaine binding site identified with [125I]RTI-55 in membranes prepared from human, monkey and guinea pig caudate

Synapse ◽  
1998 ◽  
Vol 28 (4) ◽  
pp. 322-338 ◽  
Author(s):  
Richard B. Rothman ◽  
Mayme L. Silverthorn ◽  
John R. Glowa ◽  
Dorota Matecka ◽  
Kenner C. Rice ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Binding Site ◽  
Biogenic Amine ◽  
Biogenic Amine Transporters

scholarly journals Functional characterization of the biogenic amine transporter system on human macrophages

2021 ◽  
Author(s):  
Phillip M Mackie ◽  
Adithya Gopinath ◽  
Dominic M Montas ◽  
Alyssa Nielsen ◽  
Rachel Nolan ◽  
...  
Keyword(s):  
Biogenic Amine ◽  
Functional Characterization ◽  
Human Monocyte ◽  
Paracrine Signaling ◽  
Macrophage Response ◽  
Reverse Transport ◽  
The Central Nervous System ◽  
Health And Disease

AbstractMonocyte-derived macrophages are key players in tissue homeostasis and disease regulated by a variety of signaling molecules. Recent literature has highlighted the ability for biogenic amines to regulate macrophage functions, but the mechanisms governing biogenic amine signaling on and around immune cells remains nebulous. In the central nervous system, biogenic amine transporters are regarded as the master regulators of neurotransmitter signaling. While we and others have shown macrophages express these transporters, relatively little is known of their function on these cells. To address these knowledge gaps, we interrogated the function of norepinephrine (NET) and dopamine (DAT) transporters on human monocyte-derived macrophages. We found that both NET and DAT are present and can uptake substrate from the extracellular space at baseline. Not only was DAT expressed in cultured macrophages, but it was also detected in a subset of intestinal macrophages in situ. Surprisingly, we discovered a NET-independent, DAT-mediated immuno-modulatory mechanism in response to lipopolysaccharide (LPS). LPS induced reverse transport of dopamine through DAT, engaging autocrine/paracrine signaling loop that regulated the macrophage response. Removing this signaling loop enhanced the pro-inflammatory response to LPS. Finally, we found that this DAT-immune axis was disrupted in disease. Collectively, our data introduce a novel role for DAT in the regulation of innate immunity during health and disease.

Studies of the biogenic amine transporters. 10. Characterization of a novel cocaine binding site in brain membranes prepared from dopamine transporter knockout mice

Synapse ◽  
2002 ◽  
Vol 44 (2) ◽  
pp. 94-105 ◽  
Author(s):  
Richard B. Rothman ◽  
F. Ivy Carroll ◽  
Marisela Morales ◽  
Daniel L. Rowley ◽  
Kenner C. Rice ◽  
...  
Keyword(s):  
Binding Site ◽  
Dopamine Transporter ◽  
Biogenic Amine ◽  
Knockout Mice ◽  
Brain Membranes ◽  
Biogenic Amine Transporters

Functional characterization of human brown adipose tissue metabolism

2020 ◽  
Vol 477 (7) ◽  
pp. 1261-1286 ◽  
Author(s):  
Marie Anne Richard ◽  
Hannah Pallubinsky ◽  
Denis P. Blondin
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Functional Characterization ◽  
Human Infants ◽  
Positron Emission ◽  
Brown Adipose ◽  
Treatment Of Obesity ◽  
And Function

Brown adipose tissue (BAT) has long been described according to its histological features as a multilocular, lipid-containing tissue, light brown in color, that is also responsive to the cold and found especially in hibernating mammals and human infants. Its presence in both hibernators and human infants, combined with its function as a heat-generating organ, raised many questions about its role in humans. Early characterizations of the tissue in humans focused on its progressive atrophy with age and its apparent importance for cold-exposed workers. However, the use of positron emission tomography (PET) with the glucose tracer [18F]fluorodeoxyglucose ([18F]FDG) made it possible to begin characterizing the possible function of BAT in adult humans, and whether it could play a role in the prevention or treatment of obesity and type 2 diabetes (T2D). This review focuses on the in vivo functional characterization of human BAT, the methodological approaches applied to examine these features and addresses critical gaps that remain in moving the field forward. Specifically, we describe the anatomical and biomolecular features of human BAT, the modalities and applications of non-invasive tools such as PET and magnetic resonance imaging coupled with spectroscopy (MRI/MRS) to study BAT morphology and function in vivo, and finally describe the functional characteristics of human BAT that have only been possible through the development and application of such tools.

Identification and Functional Characterization of CYP2D6*10 and CYP2D6*15 in the Uighur population

2010 ◽  
Vol 34 (8) ◽  
pp. S34-S34
Author(s):  
SiFan XU ◽  
XuCong Man ◽  
ShuChun Li
Keyword(s):  
Functional Characterization

Identification and functional characterization of a homozygous, inactivating CaSR mutation in a patient with rickets

2013 ◽  
Vol 121 (03) ◽  
Author(s):  
D Szczawinska ◽  
B Mayr ◽  
S Letz ◽  
R Rus ◽  
D Schnabel ◽  
...  
Keyword(s):  
Functional Characterization
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