scholarly journals MECHANISM OF INTRINSIC FACTOR ACTION IN EVERTED SACS OF RAT SMALL INTESTINE*

1959 ◽  
Vol 38 (1 Pt 1-2) ◽  
pp. 102-109 ◽  
Author(s):  
Victor Herbert
Keyword(s):  
Small Intestine ◽  
Intrinsic Factor ◽  
Rat Small Intestine ◽  
Everted Sacs

scholarly journals Metabolism of ε-(γ-L-glutamyl)-L-lysine in the rat

1975 ◽  
Vol 34 (2) ◽  
pp. 291-296 ◽  
Author(s):  
G. Raczyński ◽  
M. Snochowski ◽  
S. Buraczewski
Keyword(s):  
Small Intestine ◽  
Intestinal Wall ◽  
Rat Small Intestine ◽  
Blood Proteins ◽  
Vitro Experiment ◽  
Comparative Tests ◽  
In Vitro Experiment ◽  
Body Tissues ◽  
Everted Sacs

1. A study was made of the metabolism of ɛ-(γ-L-glutamyl)-L[4, 5-3H]lysine (GL) in the rat.2. The compound was largely absorbed from the intestine and metabolized. Labelled lysine was incorporated into blood proteins.3. In an in vitro experiment with everted sacs of rat small intestine, GL passed through the intestinal wall unchanged.4. The results of comparative tests using homogenates of different body tissues indicated that the kidneys were particularly active in hydrolysing GL. Their activity was nine times greater than that of the liver and eighteen times greater than that of the small intestine.

Uptake and Reduction of Radioactive Folate by Everted Sacs of Rat Small Intestine

1973 ◽  
Vol 33 (3) ◽  
pp. 433-438 ◽  
Author(s):  
Jacob Selhub ◽  
Hanna Brin ◽  
Nathan Grossowicz
Keyword(s):  
Small Intestine ◽  
Rat Small Intestine ◽  
Everted Sacs

A comparison of the structural integrity of several commonly used preparations of rat small intestine in vitro

1987 ◽  
Vol 73 (1) ◽  
pp. 53-59 ◽  
Author(s):  
Jane A. Plumb ◽  
David Burston ◽  
Terry G. Baker ◽  
Michael L. G. Gardner
Keyword(s):  
Small Intestine ◽  
Structural Integrity ◽  
Transport Processes ◽  
Buffer System ◽  
Rat Small Intestine ◽  
Quantitative Studies ◽  
Routine Monitoring ◽  
Anaesthetized Rats ◽  
Everted Sacs

1. The structural integrities of various preparations of rat small intestine for the study of absorption in vitro have been compared after incubation or perfusion. 2. Perfused intestines removed from anaesthetized rats, and thus never deprived of a supply of oxygen, maintain their structural integrity even after perfusion for 1 h provided that a Krebs–Henseleit bicarbonate perfusate is used. However, intestines removed from freshly killed rats show severe villus disruption and oedema after perfusion for only 20 min. 3. Extensive damage to both crypts and villi is observed in everted sacs of small intestine incubated for 20 min, regardless of the buffer system used. Intestinal rings show damage at the tips of the villi after incubation for 2 min, but otherwise remain morphologically intact; this damage is progressive with time. 4. It is concluded that the exact mode of preparation of intestinal tissue is critical for preservation of structural and functional integrity and that this is especially important in quantitative studies on transport processes. Further, it is recommended that routine monitoring of the integrity of intestinal preparations in vitro is desirable and that histological assessment is an appropriate technique.

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