Adenylate Energy Charge: A Method for the Determination of Viable Cell Mass in Dental Plaque Samples

1979 ◽  
Vol 58 (4_suppl) ◽  
pp. 2192-2197 ◽  
Author(s):  
Christopher W. Kemp
Keyword(s):  
Computer Program ◽  
Dental Plaque ◽  
Energy Charge ◽  
Cell Mass ◽  
Viable Cell ◽  
Viable Count ◽  
Adenylate Energy Charge ◽  
Interactive Computer Program ◽  
Biochemical Function

The biochemical function, adenylate energy charge (AEC), correlates with the viable count of S. mutans. AEC may be used to estimate the percent viable fraction of bacteria in dental plaque samples. An interactive computer program designed to process the AEC data is described.

Appendix: An Interactive Computer Program for the Computation of Adenylate Energy Charge

1979 ◽  
Vol 25 (9) ◽  
pp. 1653-1654 ◽  
Author(s):  
Christopher W Kemp ◽  
Stanley A Robrish ◽  
William H Bowen
Keyword(s):  
Computer Program ◽  
Energy Charge ◽  
Adenylate Energy Charge ◽  
Interactive Computer Program

Viable and total cell masses in dental plaque as measured by bioluminescence methods.

1979 ◽  
Vol 25 (9) ◽  
pp. 1649-1653 ◽  
Author(s):  
S A Robrish ◽  
C W Kemp ◽  
D E Chopp ◽  
W H Bowen
Keyword(s):  
Pure Culture ◽  
Dental Plaque ◽  
Adenine Nucleotide ◽  
Energy Charge ◽  
Total Cell ◽  
Small Samples ◽  
Adenylate Energy Charge ◽  
Duration Of Treatment ◽  
Nucleotide Content

Abstract Bioluminescence methods have been applied to the measurement of the viable and total cell masses of small samples of dental plaque. The total adenine nucleotide content of dental plaque samples and of a pure culture of bacteria was determined and the adenylate energy charge calculated from this. When a pure culture of bacteria was killed with heat, the adenylate energy charge decreased exponentially with duration of treatment and corresponded with a decrease in the count of viable organisms.

Adenosine Triphosphate Content of Human Dental Plaque as a Measure of Viable Cell Mass

1980 ◽  
Vol 14 (5) ◽  
pp. 265-268 ◽  
Author(s):  
W. Distler ◽  
A. Kröncke ◽  
G. Maurer
Keyword(s):  
Adenosine Triphosphate ◽  
Dental Plaque ◽  
Cell Mass ◽  
Viable Cell

scholarly journals Determination of Adenylate Nucleotides in Amphipod Gammarus fossarum by Ion-Pair Reverse Phase Liquid Chromatography: Possibilities of Positive Pressure Micro-Solid Phase Extraction

Separations ◽  
2021 ◽  
Vol 8 (2) ◽  
pp. 20
Author(s):  
Zuzana Redžović ◽  
Marijana Erk ◽  
Ema Svetličić ◽  
Lucija Dončević ◽  
Sanja Gottstein ◽  
...  
Keyword(s):  
Critical Role ◽  
Energy Charge ◽  
Adenosine Monophosphate ◽  
Ion Pair ◽  
Metabolic Energy ◽  
Positive Pressure ◽  
Adenylate Energy Charge ◽  
Reverse Phase ◽  
Gammarus Fossarum

Adenine nucleotides—adenosine monophosphate, diphosphate, and triphosphate—are of utmost importance to all living organisms, where they play a critical role in the energy metabolism and are tied to allosteric regulation in various regulatory enzymes. Adenylate energy charge represents the precise relationship between the concentrations of adenosine monophosphate, diphosphate, and triphosphate and indicates the amount of metabolic energy available to an organism. The experimental conditions of adenylate extraction in freshwater amphipod Gammarus fossarum are reported here for the first time and are crucial for the qualitative and quantitative determination of adenylate nucleotides using efficient and sensitive ion-pair reverse phase LC. It was shown that amphipod calcified exoskeleton impeded the neutralization of homogenate. The highest adenylate yield was obtained by homogenization in perchloric acid and subsequent addition of potassium hydroxide and phosphate buffer to achieve a pH around 11. This method enables separation and accurate detection of adenylates. Our study provides new insight into the complexity of adenylate extraction and quantification that is crucial for the application of adenylate energy charge as a confident physiological measure of environmental stress and as a health index of G. fossarum.

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