A New Approach to Three-dimensional Reconstructed Imaging of Hormone-secreting Cells and Their Microvessel Environments in Rat Pituitary Glands by Confocal Laser Scanning Microscopy

There has been considerable interest in the relationship between hormone-secreting endocrine cells and their microvessels in human pituitary gland. However, microcirculatory networks have rarely been studied in three dimensions (3D). This study was designed to visualize and to reveal the relationship between hormone-secreting endocrine cells and their microvessel environment in 3D, using rat pituitary glands under various (hyper/hypo) experimental conditions by confocal laser scanning microscopy (CLSM). Female adult Wistar rats were used after bilateral adrenalectomy or ACTH administration for 2 weeks. Clear 3D reconstructed images of ACTH cells, the microvessel network and counterstained nuclei were obtained at a maximal focus depth of 1 mm by CLSM without any background noise. In the hyperfunctional state, slender cytoplasmic processes of hypertrophic stellate ACTH cells frequently extended to the microvessels. In the hypofunctional state, ACTH cells appeared atrophic and round with scanty cytoplasm, and cytoplasmic adhesions to microvessel network patterns were inconspicuous. Therefore, 3D reconstructed imaging by CLSM is a useful technique with which to investigate the microvessel environment of hormone-secreting cells and has the potential to reveal dynamic hormone-secreting pathways.

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Application of confocal laser scanning microscopy (CLSM) to visualize prolactin (PRL) and PRLmRNA in the normal and estrogen-treated rat pituitary glands using non-fluorescentprobes

Microscopy Research and Technique ◽

10.1002/(sici)1097-0029(19971015)39:2<157::aid-jemt7>3.0.co;2-i ◽

1997 ◽

Vol 39 (2) ◽

pp. 157-167 ◽

Cited By ~ 21

Author(s):

Johbu Itoh ◽

Naoko Sanno ◽

Akira Matsuno ◽

Yoshiko Itoh ◽

Keiichi Watanabe ◽

...

Keyword(s):

Confocal Laser Scanning Microscopy ◽

Laser Scanning ◽

Laser Scanning Microscopy ◽

Confocal Laser Scanning ◽

Scanning Microscopy ◽

Confocal Laser ◽

Rat Pituitary ◽

Pituitary Glands

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Shrinkage of Tracheid Cells With Desorption Visualized by Confocal Laser Scanning Microscopy

IAWA Journal ◽

10.1163/22941932-90001615 ◽

2007 ◽

Vol 28 (1) ◽

pp. 29-37 ◽

Cited By ~ 16

Author(s):

Hiroki Sakagami ◽

Junji Matsumura ◽

Kazuyuki Oda

Keyword(s):

Confocal Laser Scanning Microscopy ◽

Cell Shape ◽

Laser Scanning ◽

Pinus Densiflora ◽

Laser Scanning Microscopy ◽

Confocal Laser Scanning ◽

Scanning Microscopy ◽

Confocal Laser ◽

Environment Chamber ◽

The Relationship

Confocal laser scanning microscopy (CLSM) was applied as a new method of visualizing the shrinkage of wood and its anisotropy. Control of relative humidity and temperature in a specialized environment chamber made it possible to acquire transverse images of tracheids of Akamatsu (Pinus densiflora) from the saturated condition to the dried condition. The shrinkage of tracheid cells was also determined by measuring the tangential diameter of tracheid and lumen, the radial diameter of tracheid and lumen, and the thickness of tangential and radial walls. Moreover, this technique makes it possible to discuss the relationship between moisture content and tracheid cell shape. We found the CLSM technique to be an effective method for visualizing shrinkage of tracheid cells with desorption.

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Embryological development of the freshwater crab Esanthelphusa nani (Naiyanetr, 1984) (Brachyura: Gecarcinucidae) using confocal laser scanning microscopy

Journal of Crustacean Biology ◽

10.1093/jcbiol/ruaa002 ◽

2020 ◽

Vol 40 (2) ◽

pp. 162-171

Author(s):

Rachata Maneein ◽

Camille Martinand-Mari ◽

Julien Claude ◽

Jirarach Kitana ◽

Noppadon Kitana

Keyword(s):

Confocal Laser Scanning Microscopy ◽

Laser Scanning ◽

Laser Scanning Microscopy ◽

Confocal Laser Scanning ◽

Scanning Microscopy ◽

Confocal Laser ◽

Embryological Development ◽

The Relationship ◽

Evolutionary And Developmental Biology ◽

Potential Area

Abstract We investigated the embryological development of Esanthelphusa nani (Naiyanetr, 1984), a common rice-field crab in northern Thailand, using confocal laser scanning microscopy. The development of E. nani can be completed in eggs within 12 d resulting in a hatchling stage corresponding to the megalopa stage. Pre-organogenetic stages were characterized by a superficial cleavage, including egg cleavage, egg blastula, and egg gastrula. Organogenesis stages were identified by the appearances of appendages and was divided into egg nauplius, egg zoea, and egg megalopa. Crabs metamorphose to juveniles after hatching. By providing new comparative data, our study shed some new light on the relationship between environment, phylogeny, and development, opening a potential area of research from the perspective of ecological, evolutionary, and developmental biology.

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