Gamma-Glutamyl Transferase: Applications in Forensic Pathology: I: Study of Blood Serum Recovered from Human Bodies

1987 ◽  
Vol 27 (3) ◽  
pp. 152-160 ◽  
Author(s):  
M. Piette ◽  
G. De Schrijver
Keyword(s):  
Body Fluid ◽  
Sampling Site ◽  
Chronic Alcoholism ◽  
Gamma Glutamyl Transferase ◽  
Post Mortem ◽  
Chronic Alcohol ◽  
Gamma Glutamyl ◽  
Liver Degeneration ◽  
Glutamyl Transferase

In order to appreciate more adequately chronic alcohol use by the deceased in the course of a medico-legal autopsy we studied the usefulness of a determination of gamma-glutamyl transferase (GGT) activity in cadaver serum. Some preliminary tests were performed to check the post-mortem stability of this enzyme, the influence of post-mortem haemolysis upon enzyme determination, the influence of the sampling site upon the enzyme activity and the suitability of vitreous humour as an alternative body fluid for GGT determination. Secondly, correlations between GGT activity and the degree of fatty liver degeneration and fibrosis (both possibly alcohol-induced tissue lesions) were searched for. Finally, a comparison was made between strictly selected groups of alcoholics and non-alcoholics. We concluded that, keeping in mind the necessary precautions to avoid typical post-mortem fallacies, a GGT determination in cadaver serum provides a useful parameter for detecting chronic alcoholism at the forensic autopsy.

Gamma-Glutamyl Transferase: Applications in Forensic Pathology II: Histochemical Study of Post-Mortem Human Liver Samples

1987 ◽  
Vol 27 (3) ◽  
pp. 161-169 ◽  
Author(s):  
M. Piette ◽  
Ch. Devos ◽  
B. De Prest
Keyword(s):  
Liver Tissue ◽  
Human Liver ◽  
False Negative ◽  
Chronic Alcoholism ◽  
Forensic Autopsy ◽  
Hepatic Tissue ◽  
Gamma Glutamyl Transferase ◽  
Post Mortem ◽  
Gamma Glutamyl ◽  
Glutamyl Transferase

In part I of this paper the role of a serum gamma-glutamyl transferase (GGT) determination as a marker of chronic alcoholism in the course of a forensic autopsy was analysed and discussed. To enhance the reliability of this GGT determination in cadaver serum, the suitability of a histochemical method for detecting chronic alcoholism by means of this enzyme in human liver tissue was analysed. As a preliminary test, the post-mortem stability of the GGT enzyme was observed by storing liver samples in vitro. Subsequently, correlations between liver-staining and the degree of fatty liver degeneration and fibrosis (both ethanol-induced lesions) were searched for. A comparison was then made between selected groups of alcoholics and non-alcoholics. Finally, the correlation between serum GGT activity and liver tissue staining was made. We conclude that, keeping in mind the possibility of both false positive and false negative results, a histochemical test for GGT in hepatic tissue is a further tool for detecting chronic alcoholism at the forensic autopsy. The combined examination of GGT in serum and in liver tissue is emphasized.

Development of Radioimmunoassay for Gamma-Glutamyl Transferase Using Pancreatic Enzyme

1983 ◽  
Vol 20 (4) ◽  
pp. 247-250 ◽  
Author(s):  
Masanobu Masuike ◽  
Michio Ogawa ◽  
Takeshi Kitahara ◽  
Atsuo Murata ◽  
Kazuhiko Matsuda ◽  
...  
Keyword(s):  
Enzyme Assay ◽  
Pancreatic Enzyme ◽  
Gamma Glutamyl Transferase ◽  
Gamma Glutamyl ◽  
Standard Curve ◽  
Normal Individuals ◽  
The Mean ◽  
Glutamyl Transferase ◽  
Dilution Curves

A radioimmunoassay (RIA) for the determination of gamma-glutamyl transferase (GGT) was developed using human pancreatic enzyme as antigen. The assay allows the determination of GGT in concentrations as low as 80 ng/ml, and it is reproducible and specific. A good parallel relation was demonstrated between the standard curve and dilution curves for serum, urine, bile, and partially purified kidney GGT. In normal individuals, the mean serum concentration of GGT determined by RIA was found to be 3·43 μg/ml (SD ± 1·20). Enzyme activity calculated from the GGT concentration measured by the radioimmunoassay using a regression equation was approximately twice as great as that determined by conventional enzyme assay.

The micro-determination of serum gamma-glutamyl transferase

1994 ◽  
Vol 14 (2) ◽  
pp. 81-84
Author(s):  
Zhen Hong-tao ◽  
Tan Shen-wei ◽  
Cai Xi-lu ◽  
He Shan-shu
Keyword(s):  
Gamma Glutamyl Transferase ◽  
Gamma Glutamyl ◽  
Glutamyl Transferase

scholarly journals Impact of the nature of anticoagulant on the determination of gamma glutamyl transferase activity in blood

2018 ◽  
Vol 2 (2) ◽  
Author(s):  
Mustapha ZENDJABIL ◽  
Ismahene RIKAI ◽  
Ghizlene BENHAMED
Keyword(s):  
Transferase Activity ◽  
Gamma Glutamyl Transferase ◽  
Consommation D’Alcool ◽  
Gamma Glutamyl ◽  
Diamine Tetraacetate ◽  
Glutamyl Transferase

Introduction - La gamma-glutamyl transférase (GGT) est couramment utilisée comme marqueur très sensible des maladies hépatobiliaires, de la consommation d’alcool et plus récemment, comme biomarqueur prédictif d’événements cardiovasculaires. Dans la pratique actuelle, les laboratoires mesurent l’activité de la GGT sur un tube contenant de l’héparinate de lithium. Sauf qu’il n’y a aucune preuve que l’activité de la GGT puisse être déterminée de manière fiable avec cet anticoagulant. Le but de cette étude est d’évaluer l’impact de l’anticoagulant sur la détermination de l’activité de la GGT au niveau sanguin. Matériel et méthodes - Des échantillons sanguins de 30 patients ont été recueillis sur trois types de tubes: un tube sec, un tube contenant de l’héparinate de lithium et un autre contenant de l’éthylène diamine tétraacétate de dipotassium (K2-EDTA). L’analyse statistique comprend des études de corrélation, l’analyse de Bland Altman, ainsi que la comparaison des biais avec la limite de changement analytique (LCA) et la valeur de changement de référence (VCR). Résultats - Les résultats obtenus sur les plasmas héparinés sont globalement surestimés comparativement au sérum (p = 0.001). Tandis que les résultats obtenus sur les plasmas EDTA sont plus comparables à ceux du sérum (p = 0.356). En prenant le tube sec comme référence, le biais calculé pour les échantillons de plasmas héparinés (+ 49.55%) est plus élevé que la LCA (5.24%) et la VCR (37.52%). En ce qui concerne le K2-EDTA, ce biais (-13.40%) est inférieur à la VCR, mais supérieur à la LCA. Conclusion - Nos résultats montrent que la détermination de l’activité de la GGT doit être effectuée sur des échantillons sériques de préférence. Si jamais du plasma devait être utilisé, nous recommandons d’utiliser l’EDTA comme anticoagulant.

Dynamic Changes of Serum Gamma-Glutamyl Transferase in Chronic Alcoholism

Enzyme ◽  
1979 ◽  
Vol 24 (4) ◽  
pp. 217-223 ◽  
Author(s):  
Fedora Horner ◽  
J.A. Kellen ◽  
E. Klinstone ◽  
N. Maharaj ◽  
A. Malkin
Keyword(s):  
Chronic Alcoholism ◽  
Gamma Glutamyl Transferase ◽  
Dynamic Changes ◽  
Gamma Glutamyl ◽  
Glutamyl Transferase
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