scholarly journals 3,4,3,4'-Tetrachlorobiphenyl-Induced Effects in the Rat Liver. II. Electron Microscopic Autoradiographic Localization of H-TCB

1989 ◽  
Vol 17 (4_part_2) ◽  
pp. 782-788 ◽  
Author(s):  
Stephen K. Durham ◽  
Abraham Brouwer
Keyword(s):  
Endoplasmic Reticulum ◽  
Endothelial Cell ◽  
Rat Liver ◽  
Kupffer Cell ◽  
Liver Cell ◽  
Morphologic Change ◽  
Cell Type ◽  
Sequential Order ◽  
Electron Microscopic ◽  
Morphological Alterations

Recent results (3) indicate that 200 mg 3,4,3′,4′-tetrachlorobiphenyl induces hepatomegaly accompanied by significant decreases in serum and hepatic retinoid content and hepatocyte morphologic alterations of proliferated and vesiculated endoplasmic reticulum and megamitochondria with paracrystalline inclusions. There was also an associated change in the number, size, and distribution of lipid droplets in hepatocytes and fat-storing cells. Electron microscopic autoradiographic techniques were utilized to determine the cellular and subcellular distribution of 3H-3,4,3′,4′-tetrachlorobiphenyl (3H-TCB) in the adult rat liver and determine if there is any relationship between subcellular morphologic change and radiolabel localization. Adult female WAG/Rij rats received a single intraperitoneal injection of 200 mg TCB/kg containing 1.85 mCi of 3H-TCB and were sacrificed at 1, 3, 7, and 14 days following exposure. The vast majority of 3H-TCB-derived radioactivity was located in the hepatocyte at all time points examined, ranging from 79–86% of the total number of autoradiographic grains counted over the liver cells. Sequential order of radiolabel localization per liver cell type at 1, 3, and 7 days was hepatocyte > > > Kupffer cell > fat-storing cell > endothelial cell. At day 14, the sequential order of radiolabel localization per liver cell type was hepatocyte > > > fat-storing cell > Kupffer cell > endothelial cell, which indicates that there was some shift movement of label over time. The lipid droplet, mitochondria, and endoplasmic reticulum were the subcellular structures or organelles of hepatocytes having the highest number of 3H-TCB-derived grains at all time periods examined. The predominant morphological alterations induced following TCB intoxication were observed in these organelles. The results of this study suggests that there is an association between TCB localization and morphologic change induced in mitochondria and endoplasmic reticulum of hepatocytes following TCB exposure.

scholarly journals A procedure for light and electron microscopic intracellular immunolocalization of collagen and fibronectin in rat liver.

1985 ◽  
Vol 33 (5) ◽  
pp. 407-414 ◽  
Author(s):  
B Clement ◽  
M Rissel ◽  
S Peyrol ◽  
Y Mazurier ◽  
J A Grimaud ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endoplasmic Reticulum ◽  
Rat Liver ◽  
Type Iii Collagen ◽  
Collagen Types ◽  
Electron Microscopic ◽  
Experimental Conditions ◽  
The Matrix ◽  
Matrix Components ◽  
Indirect Immunoperoxidase

Experimental conditions have been designed that permit both extracellular and intracellular immunolocalization of various collagen types and fibronectin in rat liver. The procedure involves paraformaldehyde fixation by perfusion of the organ, use of saponin as a membrane permeabilizing agent, and visualization of the matrix components by indirect immunoperoxidase. Intracellular demonstration of collagens was particularly sensitive to the composition of the fixative and the duration of fixation. Hepatocytes contained fibronectin and types I and IV collagen, whereas fat-storing and endothelial cells evidenced type III collagen in addition. All the components were specifically located in the endoplasmic reticulum and/or the Golgi apparatus.

scholarly journals Relationship between peroxisomes and endoplasmic reticulum investigated by combined catalase and glucose-6-phosphatase cytochemistry.

1981 ◽  
Vol 29 (11) ◽  
pp. 1263-1272 ◽  
Author(s):  
H Shio ◽  
P B Lazarow
Keyword(s):  
Endoplasmic Reticulum ◽  
Nuclear Envelope ◽  
Rat Liver ◽  
Reaction Products ◽  
Lead Phosphate ◽  
Electron Microscopic ◽  
Phosphatase Cytochemistry ◽  
Electron Microscopic Cytochemistry ◽  
Cellular Compartments

The theoretical advantages of electron microscopic cytochemistry were utilized to look for evidence of possible connections between peroxisomes and the endoplasmic reticulum in rat liver. Established cytochemical procedures for catalase (peroxisomes) and glucose-6-phosphatase (endoplasmic reticulum) were carried out, and evidence was sought of diffusion of reaction products between the organelles. No such diffusion was observed: lead phosphate was found in the endoplasmic reticulum and in the nuclear envelope but not in peroxisomes; oxidized diaminobenzidine (DAB) was seen only in peroxisomes. In addition, both types of cytochemistry were carried out on the same tissue. The two kinds of reaction product could be distinguished by virtue of their different electron opacities. No mixing of the two reaction products was observed. These results do not support the hypothesis that peroxisomes and endoplasmic reticulum may be connected; rather, they support the idea that the two organelles exist as separate cellular compartments.

Electron microscopic tomography of rat-liver mitochondria and their interactions with the endoplasmic reticulum

BioFactors ◽  
1998 ◽  
Vol 8 (3-4) ◽  
pp. 225-228 ◽  
Author(s):  
Carmen A. Mannella ◽  
Karolyn Buttle ◽  
Bimal K. Rath ◽  
M. Marko
Keyword(s):  
Endoplasmic Reticulum ◽  
Rat Liver ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Electron Microscopic

Amelioration of Sinusoidal Endothelial Cell Damage by Kupffer Cell Blockade during Cold Preservation of Rat Liver

1997 ◽  
Vol 72 (1) ◽  
pp. 36-48 ◽  
Author(s):  
Mototaka Niwano ◽  
Shigeki Arii ◽  
Kazunobu Monden ◽  
Satoshi Ishiguro ◽  
Toshio Nakamura ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Rat Liver ◽  
Kupffer Cell ◽  
Cell Damage ◽  
Sinusoidal Endothelial Cell ◽  
Endothelial Cell Damage ◽  
Cold Preservation

scholarly journals THE ENDOPLASMIC RETICULUM OF GASTRIC PARIETAL CELLS

1961 ◽  
Vol 11 (2) ◽  
pp. 333-347 ◽  
Author(s):  
Susumu Ito
Keyword(s):  
Endoplasmic Reticulum ◽  
Continuous System ◽  
Parietal Cells ◽  
Secretory Cells ◽  
Cell Type ◽  
Electron Microscopic ◽  
Thin Sections ◽  
Oxyntic Cell ◽  
Lower Vertebrates ◽  
Gastric Parietal Cells

An electron microscopic survey has been made of the gastric parietal or oxyntic cell of the human, cat, beaver, dog, hamster, rat, mouse, and bat, and of the corresponding cell type in two species of frog, two species of toad, and the horned lizard. A feature consistently found in the parietal cells of the mammals or their equivalent in the lower vertebrates is the agranular endoplasmic reticulum, which takes the form of branching and anastomosing small tubules approximately 200 to 500 A in diameter, sometimes expanded into flattened cisternae. In mammalian parietal cells this form of the endoplasmic reticulum is found only in limited amounts, but in the corresponding secretory cells of the amphibia and reptilia the tubular agranular reticulum is abundant. It is believed to comprise a more or less continuous system of channels, but owing to their tortuous course only short profiles are seen in thin sections. Immediately subjacent to the plasmalemma at the free surface, the cytoplasm is relatively free of organelles but is occasionally traversed by the agranular reticulum, which appears to be continuous at some points with the cell surface. The possible participation of the agranular endoplasmic reticulum in hydrochloric acid secretion is discussed.

Synergism between platelets and leukocytes in inducing endothelial cell apoptosis in the cold ischemic rat liver: a Kupffer cell mediated injury

2001 ◽  
Vol 15 (7) ◽  
pp. 1230-1232 ◽  
Author(s):  
David Sindram ◽  
Robert J. Porte ◽  
Maureane R. Hoffman ◽  
Rex C. Bentley ◽  
Pierre-Alain Clavien
Keyword(s):  
Endothelial Cell ◽  
Rat Liver ◽  
Kupffer Cell ◽  
Cell Apoptosis ◽  
Endothelial Cell Apoptosis

Electron Microscopic Characterization of Insect Hemocytes

1968 ◽  
Vol 26 ◽  
pp. 68-69 ◽  
Author(s):  
Gertraude Wittig
Keyword(s):  
Endoplasmic Reticulum ◽  
Amorphous Material ◽  
Cell Type ◽  
Electron Microscopic ◽  
Ultrathin Sections ◽  
The Subject ◽  
Insect Hemocytes ◽  
Spherical Cells ◽  
Army Worm

The fine structure of insect hemocytes has been the subject of very few investigations. In particular, the hemocytes of Lepidoptera have received almost no attention. The study presented here was carried out on the armyworm, Pseudaletia unipuncta. Hemocytes of the larva were fixed 2 to 4 days after molt to the sixth instar and studied in ultrathin sections.Microplasmatocytes (Fig. 1) were the most important phagocytes of army-worm hemolymph. They were relatively small, spherical cells with a small, round or lobed nucleus. Distensions of the perinuclear cisterna (p) were frequent and sometimes continuous with the rough endoplasmic reticulum (e). The latter formed greatly distended cisternae which almost filled the whole cytoplasm. The cisternae contained an amorphous material which appeared to be condensed in certain sacs (at e). Mitochondria (m) were rare, and they had tubular cristae. Up to four Golgi complexes (g) were identified in a microplasmatocyte section. Structured granules (sg) were specific for this cell type. Microfibrils (f) traversed the whole cytoplasm but were most frequent around the nucleus (N) and under the cell membrane.

Intact endoplasmic reticulum and albumin synthesis in rat liver cell suspensions

1971 ◽  
Vol 67 (1) ◽  
pp. 27-32 ◽  
Author(s):  
K. Weigand ◽  
M. Müller ◽  
J. Urban ◽  
G. Schreiber
Keyword(s):  
Endoplasmic Reticulum ◽  
Rat Liver ◽  
Liver Cell ◽  
Cell Suspensions ◽  
Albumin Synthesis

A Rapidly Sedimenting Fraction of Rat Liver Endoplasmic Reticulum

1973 ◽  
Vol 13 (2) ◽  
pp. 447-459 ◽  
Author(s):  
J. A. LEWIS ◽  
J. R. TATA
Keyword(s):  
Endoplasmic Reticulum ◽  
Rat Liver ◽  
Microsomal Fraction ◽  
Close Association ◽  
Balance Sheet ◽  
Subcellular Fractionation ◽  
Electron Microscopic ◽  
Microsomal Preparation ◽  
Low Speed ◽  
Microscopic Studies

Balance-sheet experiments carried out to account for the distribution of endoplasmic reticulum fragments during subcellular fractionation of rat liver showed that a large proportion of these fragments are present in the pellets of low-speed centrifugation. Using glucose-6-phosphatase and RNA as markers we found that approximately 50% of the fragments of endoplasmic reticulum sedimented in the pellet of a 640-g centrifugation, 10% in that of a 6000-g centrifugation and 35% in the pellet of a 105000-g centrifugation. Starvation of the animals before use did not alter this distribution, nor did the use of more vigorous homogenization conditions. We have developed a procedure for removing nuclei and erythrocytes from the material sedimenting at 640g to give a fraction (rapidly sedimenting ER fraction or RS-ER) similar to the standard microsomal preparation. Centrifugation of this RS-ER fraction over 1.3 M sucrose yields subfractions of high and low RNA content analogous to the rough and smooth microsomal fractions. Electron-microscopic studies showed that, whereas the rough microsomal fraction consisted of ribosome-studded vesicles of varying size and content density, the rough RS-ER fraction contained a mixture of mitochondria and double lamellar membranes with ribosomes attached. These double lamellar membranes closely resemble the endoplasmic reticulum of intact rat liver. The double lamellar membranes are frequently observed grouped in stacks and in close association with the mitochondria. The significance of the association between endoplasmic reticulum and mitochondria of the RS-ER fraction and the relation between it and the standard microsomal preparation are discussed.

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