Immunologic Detection of Occult Primary Cancer of the Head and Neck

1981 ◽  
Vol 89 (2) ◽  
pp. 230-234 ◽  
Author(s):  
H. Bryan Neel ◽  
Gary R. Pearson ◽  
Louis H. Weiland ◽  
William F. Taylor ◽  
Helmuth H. Goepfert
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Head And Neck ◽  
Epstein Barr Virus ◽  
Testing Procedure ◽  
Primary Cancer ◽  
Diagnostic Aid ◽  
Barr Virus ◽  
Antibody Titers ◽  
Epstein Barr ◽  
Obvious Source

Because antibody titers against Epstein-Barr virus (EBV) antigens often are elevated in patients with active undifferentiated and nonkeratinizing nasopharyngeal carcinoma, anti-EBV serologic tests have been applied as a diagnostic aid in patients who have metastatic lymphadenopathy in the neck without an obvious source. In this study of 44 patients, the serologic testing procedure proved useful in identifying six patients with nasopharyngeal carcinoma that was initially occult but was eventually confirmed by biopsy.

Application of Epstein-Barr Virus Serology to the Diagnosis and Staging of North American Patients with Nasopharyngeal Carcinoma

1983 ◽  
Vol 91 (3) ◽  
pp. 255-262 ◽  
Author(s):  
H. Bryan Neel ◽  
Gary R. Pearson ◽  
Louis H. Weiland ◽  
William F. Taylor ◽  
Helmut H. Goepfert ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
North American ◽  
Epstein Barr Virus ◽  
Geometric Mean ◽  
World Health ◽  
Barr Virus ◽  
Staging Systems ◽  
Antibody Titers ◽  
Epstein Barr

From 1978 to 1981, 151 patients with nasopharyngeal carcinoma (NPC) were enrolled in a prospective, collaborative study of North American patients, most of them white. Thirty-seven had World Health Organization (WHO) type 1 tumors, and 114 had WHO types 2 and 3 tumors. The anti-Epstein-Barr virus (EBV) profile of elevated antibody titers directed against viral capsid antigen and early antigen was seen in 85% of the patients with WHO types 2 and 3 tumors but in only 16% of the patients with WHO type 1 tumors. Geometric mean titers tended to be higher in higher stages of the disease in several staging systems. Low antibody-dependent cellular cytotoxicity at diagnosis appears to reflect a poorer prognosis, and the determination of antibody titers by this assay may prove to be useful for identifying persons in whom recurrent disease is likely to develop after conventional therapy. Anti-EBV titers can aid in diagnosis and treatment planning in patients with NPC, particularly those with occult primary NPC.

Nasopharyngeal carcinoma in american children: Epstein-Barr virus-specific antibody titers and prognosis

1982 ◽  
Vol 29 (2) ◽  
pp. 209-212 ◽  
Author(s):  
Robert F. Naegele ◽  
John Champion ◽  
Sharon Murphy ◽  
Gertrude Henle ◽  
Werner Henle
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Specific Antibody ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Virus Specific Antibody ◽  
Antibody Titers ◽  
Epstein Barr ◽  
American Children

Relationship between nasopharyngeal carcinoma and high antibody titers to epstein-barr virus-specific thymidine kinase

1989 ◽  
Vol 43 (1) ◽  
pp. 45-48 ◽  
Author(s):  
Mireille De Turenne-Tessier ◽  
Tadamasa Ooka ◽  
Alain Calender ◽  
Guy De The ◽  
Jacques Daillie
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Thymidine Kinase ◽  
Epstein Barr Virus ◽  
High Antibody ◽  
Barr Virus ◽  
Antibody Titers ◽  
Epstein Barr

Studies of Epstein-Barr Virus Antigens Using Immunoperoxidase and Immunofluorescence Techniques

1975 ◽  
Vol 33 ◽  
pp. 342-343
Author(s):  
R. Stephens ◽  
K. Traul ◽  
D. Woolf ◽  
P. Gaudreau
Keyword(s):  
Electron Microscopy ◽  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
If Technique ◽  
Infected Cells ◽  
Virus Antigens ◽  
Epstein Barr ◽  
Virus Capsid Antigen ◽  
Antigen Reactivity

A number of antigens have been found associated with persistent EBV infections of lymphoblastoid cells. Identification and localization of these antigens were principally by immunofluorescence (IF) techniques using sera from patients with nasopharyngeal carcinoma (NPC), Burkitt lymphoma (BL), and infectious mononucleosis (IM). Our study was mainly with three of the EBV related antigens, a) virus capsid antigen (VCA), b) membrane antigen (MA), and c) early antigens (EA) using immunoperoxidase (IP) techniques with electron microscopy (EM) to elucidate the sites of reactivity with EBV and EBV infected cells.Prior to labeling with horseradish peroxidase (HRP), sera from NPC, IM, and BL cases were characterized for various reactivities by the indirect IF technique. Modifications of the direct IP procedure described by Shabo and the indirect IP procedure of Leduc were made to enhance penetration of the cells and preservation of antigen reactivity.

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